165 research outputs found
Alteration of pathogenicity-linked life-history traits by resistance of its host Solanum tuberosum impacts sexual reproduction of the plant pathogenic oomycete Phytophthora infestans.
International audienceAlthough sexual reproduction implies a cost, it represents an evolutionary advantage for the adaptation and survival of facultative sexual pathogens. Understanding the maintenance of sex in pathogens requires to analyse how host resistance will impact their sexual reproduction through the alteration of their life-history traits. We explored this experimentally using potato (Solanum tuberosum) and one of its pathogens, the heterothallic oomycete Phytophthora infestans. Sexual reproduction was highest on hosts favouring asexual multiplication of the pathogen, suggesting similar nutritional requirements for both sexual and asexual sporulation. Sexual reproduction was also highest on hosts decreasing the latent period, probably because of a trade-off between growth and reproduction. Distinguishing host effects on each pathogenic trait remains however uneasy, as most life-history traits linked to pathogenicity were not independent of each other. We argue that sexual reproduction of P. infestans is an adaptation to survive when the host is susceptible and rapidly destroyed
A clinimetric study of lateroplusion measure by Verticam for patients recovering from a stroke
The challenge of comparing pollen-based quantitative vegetation reconstructions with outputs from vegetation models – a European perspective
We compare Holocene tree cover changes in Europe derived from a transient
Earth system model simulation (Max Planck Institute Earth System
Model – MPI-ESM1.2, including the land surface and
dynamic vegetation model JSBACH) with high-spatial-resolution time slice
simulations performed in the dynamic vegetation model LPJ-GUESS (Lund–Potsdam–Jena General Ecosystem Simulator) and
pollen-based quantitative reconstructions of tree cover based on the REVEALS (Regional Estimates of Vegetation Abundance from Large
Sites) model. The dynamic vegetation models and REVEALS agree with respect to the general temporal trends in tree cover for most parts of Europe, with a large tree cover during the mid-Holocene and a substantially smaller tree cover closer to the present time. However, the decrease in tree cover in REVEALS starts much earlier than in the models, indicating much earlier anthropogenic deforestation than the prescribed land use in the models. While LPJ-GUESS generally overestimates tree cover compared to the reconstructions, MPI-ESM indicates lower percentages of tree cover than REVEALS, particularly in central Europe and the British Isles. A comparison of the simulated climate with chironomid-based climate reconstructions reveals that model–data mismatches in tree cover are in most cases not driven by biases in the climate. Instead, sensitivity experiments indicate that the model results strongly depend on the tuning of the models regarding natural disturbance regimes (e.g. fire and wind throw). The frequency and strength of disturbances are – like most of the parameters in the vegetation models – static and calibrated to modern conditions. However, these parameter values may not be valid for past climate and vegetation states totally different from today's. In particular, the mid-Holocene natural forests were probably more stable and less sensitive to disturbances than present-day forests that are heavily altered by human interventions. Our analysis highlights the fact that such model settings are inappropriate for paleo-simulations and complicate model–data comparisons with additional challenges. Moreover, our study suggests that land use is the main driver of forest decline in Europe during the mid-Holocene and late Holocene.</p
Modeling past human-induced vegetation change is a challenge – the case of Europe
Differences between pollen-based reconstructions and dynamic vegetation simulations of past vegetation change in Europe over the last seven millennia are interpreted as being due primarily to land-use change. Incorporating land use in climate and dynamic vegetation models requires new approaches
Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism
Small GTPases play a critical role in membrane traffic. Among them, Arf6 mediates transport to and from the plasma membrane, as well as phosphoinositide signalling and cholesterol homeostasis. Here we delineate the molecular basis for the link between Arf6 and cholesterol homeostasis using an inducible knockout (KO) model of mouse embryonic fibroblasts (MEFs). We find that accumulation of free cholesterol in the late endosomes/lysosomes of Arf6 KO MEFs results from mistrafficking of Niemann–Pick type C protein NPC2, a cargo of the cation-independent mannose-6-phosphate receptor (CI-M6PR). This is caused by a selective increase in an endosomal pool of phosphatidylinositol-4-phosphate (PI4P) and a perturbation of retromer, which controls the retrograde transport of CI-M6PR via sorting nexins, including the PI4P effector SNX6. Finally, reducing PI4P levels in KO MEFs through independent mechanisms rescues aberrant retromer tubulation and cholesterol mistrafficking. Our study highlights a phosphoinositide-based mechanism for control of cholesterol distribution via retromer
Tectonic interleaving along the Main Central Thrust, Sikkim Himalaya
Geochemical and geochronological analyses provide quantitative evidence about the origin, development and motion along ductile faults, where kinematic structures have been overprinted. The Main Central Thrust is a key structure in the Himalaya that accommodated substantial amounts of the India–Asia convergence. This structure juxtaposes two isotopically distinct rock packages across a zone of ductile deformation. Structural analysis, whole-rock Nd isotopes, and U–Pb zircon geochronology reveal that the hanging wall is characterized by detrital zircon peaks at c. 800–1000 Ma, 1500–1700 Ma and 2300–2500 Ma and an εNd(0) signature of –18.3 to –12.1, and is intruded by c. 800 Ma and c. 500–600 Ma granites. In contrast, the footwall has a prominent detrital zircon peak at c. 1800–1900 Ma, with older populations spanning 1900–3600 Ma, and an εNd(0) signature of –27.7 to –23.4, intruded by c. 1830 Ma granites. The data reveal a c. 5 km thick zone of tectonic imbrication, where isotopically out-of-sequence packages are interleaved. The rocks became imbricated as the once proximal and distal rocks of the Indian margin were juxtaposed by Cenozoic movement along the Main Central Thrust. Geochronological and isotopic characterization allows for correlation along the Himalayan orogen and could be applied to other cryptic ductile shear zones
Europe's lost forests: a pollen-based synthesis for the last 11,000 years
8000 years ago, prior to Neolithic agriculture, Europe was mostly a wooded continent. Since then, its forest cover has been progressively fragmented, so that today it covers less than half of Europe’s land area, in many cases having been cleared to make way for fields and pasture-land. Establishing the origin of Europe’s current, more open land-cover mosaic requires a long-term perspective, for which pollen analysis offers a key tool. In this study we utilise and compare three numerical approaches to transforming pollen data into past forest cover, drawing on >1000 14C-dated site records. All reconstructions highlight the different histories of the mixed temperate and the northern boreal forests, with the former declining progressively since ~6000 years ago, linked to forest clearance for agriculture in later prehistory (especially in northwest Europe) and early historic times (e.g. in north central Europe). In contrast, extensive human impact on the needle-leaf forests of northern Europe only becomes detectable in the last two millennia and has left a larger area of forest in place. Forest loss has been a dominant feature of Europe’s landscape ecology in the second half of the current interglacial, with consequences for carbon cycling, ecosystem functioning and biodiversity
Excess Synaptojanin 1 Contributes to Place Cell Dysfunction and Memory Deficits in the Aging Hippocampus in Three Types of Alzheimer’s Disease
The phosphoinositide phosphatase synaptojanin 1 (SYNJ1) is a key regulator of synaptic function. We first tested whether SYNJ1 contributes to phenotypic variations in familial Alzheimer’s disease (FAD) and show that SYNJ1 polymorphisms are associated with age of onset in both early- and late-onset human FAD cohorts. We then interrogated whether SYNJ1 levels could directly affect memory. We show that increased SYNJ1 levels in autopsy brains from adults with Down syndrome (DS/AD) are inversely correlated with synaptophysin levels, a direct readout of synaptic integrity. We further report age-dependent cognitive decline in a mouse model overexpressing murine Synj1 to the levels observed in human sporadic AD, triggered through hippocampal hyperexcitability and defects in the spatial reproducibility of place fields. Taken together, our findings suggest that SYNJ1 contributes to memory deficits in the aging hippocampus in all forms of AD
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