Survival of Hepatitis A Virus (HAV), Poliovirus 1, and F-Specific Coliphages in Disposable Diapers and Landfill Leachates

Fecally soiled disposable diapers are often placed in solid waste landfills for disposal purposes. However, the public health impact that these disposals methods have on humans that come in contact with fecally soiled disposable diapers or on the environment is not fully known. Fecally soiled disposable diapers and leachates were seeded with hepatitis A virus (HAV), poliovirus 1 and F-specific (F+) coliphages and incubated aerobically and/or anaerobically at 5, 25 and 40 degrees Celsius. Samples were taken at specific intervals over a 90-day period and were assayed for surviving viruses. In some cases, viruses survived >90 days (the duration of the experiment) in fecal suspensions and solid waste landfill leachates, especially at low temperatures. In both fecally soiled diapers and leachates all viruses survived longer at lower temperatures, with 1 log[10] inactivation in 30 days at 5° Celsius under all conditions. At 40° celsius, viruses were reduced to nondetectable levels (>4 log[10] reduction) in 10-30 days in diapers and in as little as 3 days in leachates. In some cases, viruses were inactivated more rapidly under anaerobic conditions rather than aerobic conditions. Also, the relative survival of individual viruses within leachate samples was not consistent. HAV had the greatest survival rate in leachate 1 at 5 and 25°C and in leachate 2 at 25°C. Poliovirus survived the best in the other leachate conditions: 40°C in both leachate 1 and 2 and at 5°C in leachate 2. Although virus survival in leachates did not display any general trend according to virus type, comparisons of virus survival in fecal suspensions versus sanitary landfill leachates indicates that viruses survive longer in fecal suspensions by as much as 7-fold. Under most conditions, persistence of F-specific coliphages, HAV and poliovirus was similar, thus suggesting that these phages may be useful enteric virus indicators.Master of Science in Public Healt

Fusion approach for remotely sensed mapping of agriculture (FARMA):A scalable open source method for land cover monitoring using data fusion

The increasing availability of very-high resolution (VHR; &lt;2 m) imagery has the potential to enable agricultural monitoring at increased resolution and cadence, particularly when used in combination with widely available moderate-resolution imagery. However, scaling limitations exist at the regional level due to big data volumes and processing constraints. Here, we demonstrate the Fusion Approach for Remotely Sensed Mapping of Agriculture (FARMA), using a suite of open source software capable of efficiently characterizing time-series field-scale statistics across large geographical areas at VHR resolution. We provide distinct implementation examples in Vietnam and Senegal to demonstrate the approach using WorldView VHR optical, Sentinel-1 Synthetic Aperture Radar, and Sentinel-2 and Sentinel-3 optical imagery. This distributed software is open source and entirely scalable, enabling large area mapping even with modest computing power. FARMA provides the ability to extract and monitor sub-hectare fields with multisensor raster signals, which previously could only be achieved at scale with large computational resources. Implementing FARMA could enhance predictive yield models by delineating boundaries and tracking productivity of smallholder fields, enabling more precise food security observations in low and lower-middle income countries.</p

Threshold neutral pion photoproduction off the tri-nucleon to O(q^4)

We calculate electromagnetic neutral pion production off tri-nucleon bound states (3H, 3He) at threshold in chiral nuclear effective field theory to fourth order in the standard heavy baryon counting. We show that the fourth order two-nucleon corrections to the S-wave multipoles at threshold are very small. This implies that a precise measurement of the S-wave cross section for neutral pion production off 3He allows for a stringent test of the chiral perturbation theory prediction for the S-wave electric multipole E_{0+}^{pi0 n}.Comment: 17 pages, 5 figures, title changed, final version to appear in EPJA. arXiv admin note: substantial text overlap with arXiv:1103.340

Quinoa Phenotyping Methodologies: An International Consensus

Quinoa is a crop originating in the Andes but grown more widely and with the genetic potential for significant further expansion. Due to the phenotypic plasticity of quinoa, varieties need to be assessed across years and multiple locations. To improve comparability among field trials across the globe and to facilitate collaborations, components of the trials need to be kept consistent, including the type and methods of data collected. Here, an internationally open-access framework for phenotyping a wide range of quinoa features is proposed to facilitate the systematic agronomic, physiological and genetic characterization of quinoa for crop adaptation and improvement. Mature plant phenotyping is a central aspect of this paper, including detailed descriptions and the provision of phenotyping cards to facilitate consistency in data collection. High-throughput methods for multi-temporal phenotyping based on remote sensing technologies are described. Tools for higher-throughput post-harvest phenotyping of seeds are presented. A guideline for approaching quinoa field trials including the collection of environmental data and designing layouts with statistical robustness is suggested. To move towards developing resources for quinoa in line with major cereal crops, a database was created. The Quinoa Germinate Platform will serve as a central repository of data for quinoa researchers globally.EEA FamailláFil: Stanschewski, Clara S. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Rey, Elodie. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Fiene, Gabriele. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Craine, Evan B. Washington State University. Department of Crop and Soil Sciences; Estados UnidosFil: Wellman, Gordon. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Melino, Vanessa J. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Patiranage, Dilan S.R. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia SauditaFil: Patiranage, Dilan S.R. Christian-Albrechts-University of Kiel. Plant Breeding Institute; AlemaniaFil: Johansen, Kasper. King Abdullah University of Science and Technology. Water Desalination and Reuse Center; Arabia SauditaFil: Schmöckel, Sandra M. University of Hohenheim. Institute of Crop Science. Department Physiology of Yield Stability; AlemaniaFil: Erazzu, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Tester, Mark. King Abdullah University of Science and Technology. Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division; Arabia Saudit

The Solution Space of the Unitary Matrix Model String Equation and the Sato Grassmannian

The space of all solutions to the string equation of the symmetric unitary one-matrix model is determined. It is shown that the string equation is equivalent to simple conditions on points $V_1$ and $V_2$ in the big cell \Gr of the Sato Grassmannian $Gr$. This is a consequence of a well-defined continuum limit in which the string equation has the simple form \lb \cp ,\cq_- \rb =\hbox{\rm 1}, with \cp and \cq_- $2\times 2$ matrices of differential operators. These conditions on $V_1$ and $V_2$ yield a simple system of first order differential equations whose analysis determines the space of all solutions to the string equation. This geometric formulation leads directly to the Virasoro constraints \L_n\,(n\geq 0), where \L_n annihilate the two modified-KdV \t-functions whose product gives the partition function of the Unitary Matrix Model.Comment: 21 page

Adherence to cancer screening guidelines in Australian survivors of allogeneic blood and marrow transplantation (BMT)

Allogeneic Blood and Marrow Transplant (BMT) survivors are at high risk of secondary cancers. Although current guidelines endorse survivors following Country-specific general population screening recommendations to mitigate this risk, little is known about cancer screening adherence in Australian BMT survivors. We conducted a cross-sectional survey of 441 BMT survivors who were >1 year post transplant, to explore rates of screening for secondary cancers and to identify barriers to cancer screening recommendations. Survey instruments included the Sydney Post-BMT Survey, FACT-BMT, DASS 21, The Chronic Graft versus Host Disease (GVHD) Activity Assessment–Patient Self-Report (Form B), the Lee Chronic GVHD Symptom Scale, Fear of Cancer Recurrence Scale, and The Post Traumatic Growth Inventory. Fifty-seven percent of respondents were male, median age 54 years, and 40% were >6 years post-BMT. Rates of cancer screening adherence were as follows: cervical 63.4%, breast 53.3%, skin 52.4%, and bowel 32.3%. Older BMT survivors and those >2 years post transplant were more likely to undergo cancer screening. Improved quality of life was associated with screening for skin, breast, and cervical cancer. Fear of cancer recurrence negatively impacted on cervical screening. For those who had not undergone screening, the majority reported not being advised to do so by their treatment team. This study is the largest and most comprehensive to date exploring cancer screening adherence in BMT survivors in Australia. These data provide the basis for health service reform to better meet the needs of BMT survivors and provide evidence to support counseling and education of both patients and professionals. Keywords: Australia, blood and marrow transplant (BMT), cancer screening, late effects, secondary cancers, survivorsfunded by the New South Wales Agency for Clinical Innovation, Blood and Marrow Transplant Network and supported by the Northern Blood Research Centr

Adherence to cancer screening guidelines in Australian survivors of allogeneic blood and marrow transplantation (BMT)

Allogeneic Blood and Marrow Transplant (BMT) survivors are at high risk of secondary cancers. Although current guidelines endorse survivors following Country-specific general population screening recommendations to mitigate this risk, little is known about cancer screening adherence in Australian BMT survivors. We conducted a cross-sectional survey of 441 BMT survivors who were >1 year post transplant, to explore rates of screening for secondary cancers and to identify barriers to cancer screening recommendations. Survey instruments included the Sydney Post-BMT Survey, FACT-BMT, DASS 21, The Chronic Graft versus Host Disease (GVHD) Activity Assessment–Patient Self-Report (Form B), the Lee Chronic GVHD Symptom Scale, Fear of Cancer Recurrence Scale, and The Post Traumatic Growth Inventory. Fifty-seven percent of respondents were male, median age 54 years, and 40% were >6 years post-BMT. Rates of cancer screening adherence were as follows: cervical 63.4%, breast 53.3%, skin 52.4%, and bowel 32.3%. Older BMT survivors and those >2 years post transplant were more likely to undergo cancer screening. Improved quality of life was associated with screening for skin, breast, and cervical cancer. Fear of cancer recurrence negatively impacted on cervical screening. For those who had not undergone screening, the majority reported not being advised to do so by their treatment team. This study is the largest and most comprehensive to date exploring cancer screening adherence in BMT survivors in Australia. These data provide the basis for health service reform to better meet the needs of BMT survivors and provide evidence to support counseling and education of both patients and professionals. Keywords: Australia, blood and marrow transplant (BMT), cancer screening, late effects, secondary cancers, survivorsfunded by the New South Wales Agency for Clinical Innovation, Blood and Marrow Transplant Network and supported by the Northern Blood Research Centr

Constraint methods for determining pathways and free energy of activated processes

Activated processes from chemical reactions up to conformational transitions of large biomolecules are hampered by barriers which are overcome only by the input of some free energy of activation. Hence, the characteristic and rate-determining barrier regions are not sufficiently sampled by usual simulation techniques. Constraints on a reaction coordinate r have turned out to be a suitable means to explore difficult pathways without changing potential function, energy or temperature. For a dense sequence of values of r, the corresponding sequence of simulations provides a pathway for the process. As only one coordinate among thousands is fixed during each simulation, the pathway essentially reflects the system's internal dynamics. From mean forces the free energy profile can be calculated to obtain reaction rates and insight in the reaction mechanism. In the last decade, theoretical tools and computing capacity have been developed to a degree where simulations give impressive qualitative insight in the processes at quantitative agreement with experiments. Here, we give an introduction to reaction pathways and coordinates, and develop the theory of free energy as the potential of mean force. We clarify the connection between mean force and constraint force which is the central quantity evaluated, and discuss the mass metric tensor correction. Well-behaved coordinates without tensor correction are considered. We discuss the theoretical background and practical implementation on the example of the reaction coordinate of targeted molecular dynamics simulation. Finally, we compare applications of constraint methods and other techniques developed for the same purpose, and discuss the limits of the approach

Enhanced terrestrial carbon export from East Antarctica during the early Eocene

Terrestrial organic carbon (TerrOC) acts as an important CO2 sink when transported via rivers to the ocean and sequestered in coastal marine sediments. This mechanism might help to modulate atmospheric CO2 levels over short- and long timescales (103 to 106 years), but its importance during past warm climates remains unknown. Here we use terrestrial biomarkers preserved in coastal marine sediment samples from Wilkes Land, East Antarctica (~67°S) to quantify TerrOC burial during the early Eocene (~54.4 to 51.5 Ma). Terrestrial biomarker distributions indicate the delivery of plant-, soil- and peat-derived organic carbon (OC) into the marine realm. Mass accumulation rates of plant- (long-chain n-alkane) and soil-derived (hopane) biomarkers dramatically increase between the earliest Eocene (~54 Ma) and the early Eocene Climatic Optimum (EECO; ~53 Ma). This coincides with increased OC mass accumulation rates and indicates enhanced TerrOC burial during the EECO. Leaf wax δ 2H values indicate that the EECO was characterised by wetter conditions relative to the earliest Eocene, suggesting that hydroclimate exerts a first-order control on TerrOC export. Our results indicate that TerrOC burial in coastal marine sediments UOB Open could have acted as an important negative feedback mechanism during the early Eocene, but also during other warm climate intervals

α/β-Hydrolase Domain 6 Deletion Induces Adipose Browning and Prevents Obesity and Type 2 Diabetes

SummarySuppression of α/β-domain hydrolase-6 (ABHD6), a monoacylglycerol (MAG) hydrolase, promotes glucose-stimulated insulin secretion by pancreatic β cells. We report here that high-fat-diet-fed ABHD6-KO mice show modestly reduced food intake, decreased body weight gain and glycemia, improved glucose tolerance and insulin sensitivity, and enhanced locomotor activity. ABHD6-KO mice also show increased energy expenditure, cold-induced thermogenesis, brown adipose UCP1 expression, fatty acid oxidation, and white adipose browning. Adipose browning and cold-induced thermogenesis are replicated by the ABHD6 inhibitor WWL70 and by antisense oligonucleotides targeting ABHD6. Our evidence suggests that one mechanism by which the lipolysis derived 1-MAG signals intrinsic and cell-autonomous adipose browning is via PPARα and PPARγ activation, and that ABHD6 regulates adipose browning by controlling signal competent 1-MAG levels. Thus, ABHD6 regulates energy homeostasis, brown adipose function, and white adipose browning and is a potential therapeutic target for obesity and type 2 diabetes