Saccharomyces cerevisiae-based system for studying clustered DNA damages

DNA-damaging agents can induce clustered lesions or multiply damaged sites (MDSs) on the same or opposing DNA strands. In the latter, attempts to repair MDS can generate closely opposed single-strand break intermediates that may convert non-lethal or mutagenic base damage into double-strand breaks (DSBs). We constructed a diploid S. cerevisiae yeast strain with a chromosomal context targeted by integrative DNA fragments carrying different damages to determine whether closely opposed base damages are converted to DSBs following the outcomes of the homologous recombination repair pathway. As a model of MDS, we studied clustered uracil DNA damages with a known location and a defined distance separating the lesions. The system we describe might well be extended to assessing the repair of MDSs with different compositions, and to most of the complex DNA lesions induced by physical and chemical agents

Geosciences Roadmap for Research Infrastructures 2025 - 2028 by the Swiss Geosciences Community

This roadmap is the product of a grassroots effort by the Swiss Geosciences community. It is the first of its kind, outlining an integrated approach to research facilities for the Swiss Geosciences. It spans the planning period 2025-2028. Swiss Geoscience is by its nature leading or highly in-volved in research on many of the major national and global challenges facing society such as climate change and meteorological extreme events, environmental pol-lution, mass movements (land- and rock-slides), earth-quakes and seismic hazards, global volcanic hazards, and energy and other natural resources. It is essential to under- stand the fundamentals of the whole Earth system to pro-vide scientific guidelines to politicians, stakeholders and society for these pressing issues. Here, we strive to gain efficiency and synergies through an integrative approach to the Earth sciences. The research activities of indivi- dual branches in geosciences were merged under the roof of the 'Integrated Swiss Geosciences'. The goal is to facilitate multidisciplinary synergies and to bundle efforts for large research infrastructural (RI) requirements, which will re-sult in better use of resources by merging sectorial acti- vities under four pillars. These pillars represent the four key RIs to be developed in a synergistic way to improve our understanding of whole-system processes and me- chanisms governing the geospheres and the interactions among their components. At the same time, the roadmap provides for the required transition to an infrastructure adhering to FAIR (findable, accessible, interoperable, and reusable) data principles by 2028.The geosciences as a whole do not primarily profit from a single large-scale research infrastructure investment, but they see their highest scientific potential for ground-break-ing new findings in joining forces in establishing state-of-the-art RI by bringing together diverse expertise for the benefit of the entire geosciences community. Hence, the recommendation of the geoscientific community to policy makers is to establish an integrative RI to support the ne- cessary breadth of geosciences in their endeavor to ad-dress the Earth system across the breadth of both temporal and spatial scales. It is also imperative to include suffi-cient and adequately qualified personnel in all large RIs. This is best achieved by fostering centers of excellence in atmospheric, environmental, surface processes, and deep Earth projects, under the roof of the 'Integrated Swiss Geosciences'. This will provide support to Swiss geo-sciences to maintain their long standing and internatio- nally well-recognized tradition of observation, monitor-ing, modelling and understanding of geosciences process-es in mountainous environments such as the Alps and beyond

Evidence from flank ridges for long-term diminishing movements of the Slumgullion landslide, Hinsdale County, Colorado

The Slumgullion landslide, in southwestern Colorado, is a complex phenomenon consisting of an active landslide currently moving on the upper-middle part of an older, larger, and inactive landslide. It is bounded for most of its length by flank ridges that are linear ridges of landslide material that have formed during movement along the lateral boundaries of the landslide. Flank ridges formed by deformation of landslide material, as well as formed by deposition of landslide material onto the adjacent ground surface, were recognized on the inactive Slumgullion landslide. Flank ridges are the best preserved features in the deposits of the inactive Slumgullion landslide. They extend continuously for several hundred meters, following the landslide boundaries. The several generations of flank ridges were mapped on aerial photographs and later field checked; observation of their main geomorphic, pedologic, and sedimentologic characters helped in reconstructing the chronology of their formation. An attempt was also made to correlate the identified sets of flank ridges with the known history of movement of the Slumgullion landslide. Maps and profiles were used to estimate previous width and thickness of the landslide at the time of formation of the identified sets of flank ridges. Comparison of these values with the present width and thickness of the Slumgullion landslide showed a thinning and narrowing trend, which will eventually result in inactivity of the presently active part of the landslide, unless a new amount of material is available from the source area

Raw material choices and material characterization of the 3rd and 2nd millennium BC pottery from the Petit‐Chasseur necropolis: Insights into the megalith‐erecting society of the Upper Rhône Valley, Switzerland

Owing to its well‐preserved and long‐lasting archaeological record, the necropolis of Petit‐Chasseur in the Upper Rhône Valley (3100–1600 BC) showcases the economic, social, and ideological changes of 3rd and 2nd millennium BC Europe excellently. An in‐depth investigation of pottery artifacts was carried out using multiple spectroscopic and microscopic techniques. Nine types of ceramic fabrics were identified based on the variety of temper and natural inclusions; however, the mineralogy and phase chemistry of the ceramic matrix showed the paste to be primarily illitic or muscovitic, irrespective of the inclusion type. Muscovitic clays were likely procured from the fluvioglacial, glaciolacustrine, colluvial, and till sediment abundantly available at higher altitudes of the Upper Rhône Valley, whereas illitic clays were acquired from pedogenized loess horizons or the Rhône River alluvium. Different raw material choices and paste preparation practices suggest distinct ceramic traditions that likely existed in the valley during the 3rd and 2nd millennia BC. This, along with the hypothesized provenance of the raw material, is likely in favor of various prehistoric communities gathering at the megalithic necropolis from close and distant parts of the valley using the Petit‐Chasseur site as a place of assembly