36 research outputs found
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Two distinct microbial communities revealed in the sponge Cinachyrella
Marine sponges are vital components of benthic and coral reef ecosystems, providing shelter and nutrition for many organisms. In addition, sponges act as an essential carbon and nutrient link between the pelagic and benthic environment by filtering large quantities of seawater. Many sponge species harbor a diverse microbial community (including Archaea, Bacteria and Eukaryotes), which can constitute up to 50% of the sponge biomass. Sponges of the genus Cinachyrella are common in Caribbean and Floridian reefs and their archaeal and bacterial microbiomes were explored here using 16S rRNA gene tag pyrosequencing. Cinachyrella specimens and seawater samples were collected from the same South Florida reef at two different times of year. In total, 639 OTUs (12 archaeal and 627 bacterial) belonging to 2 archaeal and 21 bacterial phyla were detected in the sponges. Based on their microbiomes, the six sponge samples formed two distinct groups, namely sponge group 1 (SG1) with lower diversity (Shannon-Wiener index: 3.73 ± 0.22) and SG2 with higher diversity (Shannon-Wiener index: 5.95 ± 0.25). Hosts’ 28S rRNA gene sequences further confirmed that the sponge specimens were composed of two taxa closely related to Cinachyrella kuekenthalli. Both sponge groups were dominated by Proteobacteria, but Alphaproteobacteria were significantly more abundant in SG1. SG2 harbored many bacterial phyla (>1% of sequences) present in low abundance or below detection limits (<0.07%) in SG1 including: Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospirae, PAUC34f, Poribacteria, and Verrucomicrobia. Furthermore, SG1 and SG2 only had 95 OTUs in common, representing 30.5 and 22.4% of SG1 and SG2’s total OTUs, respectively. These results suggest that the sponge host may exert a pivotal influence on the nature and structure of the microbial community and may only be marginally affected by external environment parameters.This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Frontiers Research Foundation. The published article can be found at: http://www.frontiersin.org/Microbiology.Keywords: diversity, 16S rRNA, marine sponge, microbiome, pyrosequencing, archaea, symbiont
Age-related shift in LTD is dependent on neuronal adenosine A(2A) receptors interplay with mGluR5 and NMDA receptors
Synaptic dysfunction plays a central role in Alzheimer's disease (AD), since it drives the cognitive decline. An association between a polymorphism of the adenosine A2A receptor (A2AR) encoding gene-ADORA2A, and hippocampal volume in AD patients was recently described. In this study, we explore the synaptic function of A2AR in age-related conditions. We report, for the first time, a significant overexpression of A2AR in hippocampal neurons of aged humans, which is aggravated in AD patients. A similar profile of A2AR overexpression in rats was sufficient to drive age-like memory impairments in young animals and to uncover a hippocampal LTD-to-LTP shift. This was accompanied by increased NMDA receptor gating, dependent on mGluR5 and linked to enhanced Ca(2+) influx. We confirmed the same plasticity shift in memory-impaired aged rats and APP/PS1 mice modeling AD, which was rescued upon A2AR blockade. This A2AR/mGluR5/NMDAR interaction might prove a suitable alternative for regulating aberrant mGluR5/NMDAR signaling in AD without disrupting their constitutive activity
Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling
Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal coverage along a given chromosome and between chromosomes. The identity at the DNA level between the metagenomes and the cultured genome was very high (96.3% identical bases for the three larger chromosomes over a 360 kbp alignment). At least two to three different genotypes seemed to be present in each natural sample based on read mapping to Bathycoccus RCC1105 genome
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Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton
Enhanced Survival of Escherichia coli in Subtropical Beach Sand and Implications for Water Quality Managers
Several health problems such as gastroenteritis, upper respiratory infections and skin, eye, and ear infections can be associated with swimming in polluted waters. In order to assess potential health risks, authorities regularly monitor the quality of recreational waters by analyzing for indicator organisms. Total coliforms, fecal coliforms, enterococci and E. coli, naturally found in the intestinal flora of warmblooded animals, are amongst the most frequently used bacterial indicators for sewage pollution. These organisms are thought to be representative of more virulent pathogens and their densities in the water are indicative of the amount of sewage contamination present. Current guidelines for beaches in the United States recommend routine testing of beach water samples, but risks associated with bacterial numbers in the sand have never been considered. This is surprising because many beachgoers spend most of their time around the sand.
As part of an EPA-funded study to investigate microorganisms on the beach, levels of E. coli in the sand and the water were recorded over a lO-month period at two different beaches in South Florida. Densities were consistently higher in the dry sand relative to the wet sand and sand had more bacteria than water. Mesocosm experiments conducted in the laboratory showed that, regardless of conditions, E. coli levels significantly increased within 24 h to 48 h in sterile sand but died when added to sterile seawater. Because of the high sand counts, the potential for bacteria to wash out of sand was studied at Hollywood Beach. Results showed that beach bacteria that grow in situ and wash out into the water influence the densities of E. coli in the water. This has consequences for water managers assessing beach quality. Future protocols for sampling might include water samples collected well away from the swash zone.
Attempts were made to isolate a eukaryotic pathogen, Entamoeba histolytica, from wet sand because previous work had shown that sand samples were occasionally positive for E. histolytica using a commercially available test kit. Even although Entamoeba-like organisms were present, ELISA test revealed that these were not E. histolytica.
Preliminary experiments were conducted using alternative methods for assessing fecal pollution. The levels of two types of coliphage, somatic and F-specific phage, were determined during the monthly samplings. The numbers did not generally correlate with the levels of E. coli or enterococci. Polymerase Chain Reaction, targeting pathogenic genes of E. coli, was used to screen E. coli colonies from wet sand, dry sand and sewage. Because of the surprisingly high numbers of positive results in the dry sand, further investigation will be needed to determine the significance of the results
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ThurberRebeccaMicrobiologyTwoDistinctMicrobial_SupplementaryMaterials.zip
Marine sponges are vital components of benthic and coral reef ecosystems, providing
shelter and nutrition for many organisms. In addition, sponges act as an essential carbon
and nutrient link between the pelagic and benthic environment by filtering large quantities
of seawater. Many sponge species harbor a diverse microbial community (including
Archaea, Bacteria and Eukaryotes), which can constitute up to 50% of the sponge
biomass. Sponges of the genus Cinachyrella are common in Caribbean and Floridian
reefs and their archaeal and bacterial microbiomes were explored here using 16S rRNA
gene tag pyrosequencing. Cinachyrella specimens and seawater samples were collected
from the same South Florida reef at two different times of year. In total, 639 OTUs
(12 archaeal and 627 bacterial) belonging to 2 archaeal and 21 bacterial phyla were
detected in the sponges. Based on their microbiomes, the six sponge samples formed
two distinct groups, namely sponge group 1 (SG1) with lower diversity (Shannon-Wiener
index: 3.73 ± 0.22) and SG2 with higher diversity (Shannon-Wiener index: 5.95 ± 0.25). Hosts’ 28S rRNA gene sequences further confirmed that the sponge specimens
were composed of two taxa closely related to Cinachyrella kuekenthalli. Both sponge
groups were dominated by Proteobacteria, but Alphaproteobacteria were significantly
more abundant in SG1. SG2 harbored many bacterial phyla (>1% of sequences) present
in low abundance or below detection limits (<0.07%) in SG1 including: Acidobacteria,
Chloroflexi, Gemmatimonadetes, Nitrospirae, PAUC34f, Poribacteria, and Verrucomicrobia.
Furthermore, SG1 and SG2 only had 95 OTUs in common, representing 30.5 and 22.4%
of SG1 and SG2’s total OTUs, respectively. These results suggest that the sponge host
may exert a pivotal influence on the nature and structure of the microbial community and
may only be marginally affected by external environment parameters.Keywords: symbionts, pyrosequencing, archaea, microbiome, 16S rRNA, marine sponge, diversit
Responses of the picoprasinophyte Micromonas commoda to light and ultraviolet stress
International audienceMicromonas is a unicellular marine green alga that thrives from tropical to polar ecosystems. We investigated the growth and cellular characteristics of acclimated mid-exponential phase Micromonas commoda RCC299 over multiple light levels and over the diel cycle (14:10 hour light:dark). We also exposed the light:dark acclimated M. commoda to experimental shifts from moderate to high light (HL), and to HL plus ultraviolet radiation (HL+UV), 4.5 hours into the light period. Cellular responses of this prasinophyte were quantified by flow cytometry and changes in gene expression by qPCR and RNA-seq. While proxies for chlorophyll a content and cell size exhibited similar diel variations in HL and controls, with progressive increases during day and decreases at night, both parameters sharply decreased after the HL+UV shift. Two distinct transcriptional responses were observed among chloroplast genes in the light shift experiments: i) expression of transcription and translation-related genes decreased over the time course, and this transition occurred earlier in treatments than controls; ii) expression of several photosystem I and II genes increased in HL relative to controls, as did the growth rate within the same diel period. However, expression of these genes decreased in HL+UV, likely as a photoprotective mechanism. RNA-seq also revealed two genes in the chloroplast genome, ycf2-like and ycf1-like, that had not previously been reported. The latter encodes the second largest chloroplast protein in Micromonas and has weak homology to plant Ycf1, an essential component of the plant protein translocon. Analysis of several nuclear genes showed that the expression of LHCSR2, which is involved in non-photochemical quenching, and five light-harvesting-like genes, increased 30 to >50-fold in HL+UV, but was largely unchanged in HL and controls. Under HL alone, a gene encoding a novel nitrite reductase fusion protein (NIRFU) increased, possibly reflecting enhanced N-assimilation under the 625 μmol photons m-2 s-1 supplied in the HL treatment. NIRFU’s domain structure suggests it may have more efficient electron transfer than plant NIR proteins. Our analyses indicate that Micromonas can readily respond to abrupt environmental changes, such that strong photoinhibition was provoked by combined exposure to HL and UV, but a ca. 6-fold increase in light was stimulatory