Assessing interactions, predicting function, and increasing degradation potential of a PAH-degrading bacterial consortium by effect of an inoculant strain

A natural phenanthrene-degrading consortium CON was inoculated with an exogenous strain Sphingobium sp. (ex Sp. paucimobilis) 20006FA yielding the consortium called I-CON, in order to study ecological interactions into the bacterial community. DGGE and proteomic profiles and analyses by HTS (High-Throughput Sequencing) technologies demonstrated inoculant establishment and changes on CON composition. Inoculation increased degradation efficiency in I-CON and prevented intermediate HNA accumulation. This could be explained not only by the inoculation, but also by enrichment in Achromobacter genus at expense of a decrease in Klebsiella genus. After inoculation, cooperation between Sphingobium and Achromobacter genera were improved, thereby, some competition could have been generated, and as a consequence, species in minor proportion (cheaters), as Inquilinus sp. and Luteibacter sp., were not detected. Sequences of Sphingobium (corresponding to the inoculated strain) did not vary. PICRUSt predicted a network with bacterial phylotypes connected with enzymes, showing functional redundancy in the phenanthrene pathway, with exception of the first enzymes biphenyl-2,3-diol 1,2-dioxygenase and protocatechuate 4,5-dioxygenase that were only encoded in Sphingobium sp. This is the first report where a natural consortium that has been characterized by HTS technologies is inoculated with an exogenous strain in order to study competitiveness and interactions.Fil: Macchi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Vega Vela, Nelson E.. Pontificia Universidad Javeriana; Colombia. Universidad de Bogotá Jorge Tadeo Lozano; ColombiaFil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin

Diseño definitivo de la central hidroeléctrica El Altar

En el presente trabajo se realizó el diseño de la central hidroeléctrica “El Altar” aprovechando la topografía y el caudal del río Puela que está ubicado en la parroquia El Altar, cantón Penipe, provincia de Chimborazo, para generar 10 MW de potencia eléctrica. Se realiza una investigación de los conceptos básicos relacionados con la generación hidroeléctrica, parámetros de diseño y se muestra una descripción de los elementos. Se presenta un análisis estadístico para determinar el caudal de diseño. Consecutivamente se realizan los cálculos para determinar las dimensiones de los elementos civiles y mecánicos empleados, basados en datos tomados en el sector, permitiendo establecer la captación con una bocatoma lateral, la conducción debido a la topografía de la zona estará compuesta de tubería, hasta llegar al tanque de carga que alimenta a dos tramos en paralelo de tubería de presión para llegar a las unidades generadoras ubicadas en la casa de máquinas. Posteriormente se selecciona el equipo electro mecánico y se propone la construcción de un patio de transmisión. Considerando la importancia del efecto positivo y negativo se realiza un estudio de impacto ambiental, se presenta el análisis de presupuestos y costos del proyecto. Se verifica el cumplimiento de los objetivos de la tesis, proponiendo que la energía generada sea traslada hasta la subestación más cercana y se confirma la necesidad de un trabajo conjunto entre las empresas públicas y privadas para lograr la implementación de este proyecto.The design of the hydroelectric power plant "El Altar" was carried out in this work, thanks to the topography and the flow of the river Puela which is located in the parish El Altar, Penipe canton, Chimborazo province, to generate 10 MW of electrical power. An investigation of the basic concepts related to hydroelectric generation, design parameters and a description of the elements was performed. In addition a statistical analysis was also presented to determine the design flow. Consecutively calculations were executed to determine the dimensions of the civil and mechanical elements used, based on data collected in the sector, thus allowing to establish the catchment with a lateral intake, driving due to the topography of the area will consist of pipe up to coming to the load tank which feeds two sections in parallel of pressure pipe to reach the generating units located in the machinery house. Subsequently the electro mechanical equipment was selected, and it was suggested the construction of a transmission yard. An environmental impact study was conducted considering the importance of the positive and negative effect, and the analysis of budgets and project costs was presented. the fulfillment of the objectives of the thesis was verified by proposing that the energy generated is transferred to the nearest substation and the need for a joint effort between public and private companies to archive the implementation of this project was confirmed

Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics

Two synthetic bacterial consortia (SC) composed of bacterial strains Sphingobium sp. (AM), Klebsiella aerogenes (B), Pseudomonas sp. (Bc-h and T), Burkholderia sp. (Bk) and Inquilinus limosus (Inq) isolated from a natural phenanthrene (PHN)-degrading consortium (CON) were developed and evaluated as an alternative approach to PHN biodegradation in bioremediation processes. A metabolic network showing the potential role of strains was reconstructed by in silico study of the six genomes and classification of dioxygenase enzymes using RHObase and AromaDeg databases. Network analysis suggested that AM and Bk were responsible for PHN initial attack, while Inq, B, T and Bc-h would degrade PHN metabolites. The predicted roles were further confirmed by physiological, RT-qPCR and metaproteomic assays. SC-1 with AM as the sole PHN degrader was the most efficient. The ecological roles inferred in this study can be applied to optimize the design of bacterial consortia and tackle the biodegradation of complex environmental pollutants.EEA RafaelaFil: Macchi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Nieto, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Vega-Vela, Nelson E. Pontificia Universidad Javeriana; Colombia. Universidad de Bogotá Jorge Tadeo Lozano; ColombiaFil: Junca, Howard. Microbiomas Foundation. Division Ecogenomics & Holobionts. RG Microbial Ecology: Metabolism, Genomics & Evolution; ColombiaFil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; ArgentinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Morelli, Irma S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; ArgentinaFil: Coppotelli, Bibiana M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin

Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics

Two synthetic bacterial consortia (SC) composed of bacterial strains Sphingobium sp. (AM), Klebsiella aerogenes (B), Pseudomonas sp. (Bc-h and T), Burkholderia sp. (Bk) and Inquilinus limosus (Inq) isolated from a natural phenanthrene (PHN)-degrading consortium (CON) were developed and evaluated as an alternative approach to PHN biodegradation in bioremediation processes. A metabolic network showing the potential role of strains was reconstructed by in silico study of the six genomes and classification of dioxygenase enzymes using RHObase and AromaDeg databases. Network analysis suggested that AM and Bk were responsible for PHN initial attack, while Inq, B, T and Bc-h would degrade PHN metabolites. The predicted roles were further confirmed by physiological, RT-qPCR and metaproteomic assays. SC-1 with AM as the sole PHN degrader was the most efficient. The ecological roles inferred in this study can be applied to optimize the design of bacterial consortia and tackle the biodegradation of complex environmental pollutants.Centro de Investigación y Desarrollo en Fermentaciones Industriale

The Genome of Nectria haematococca: Contribution of Supernumerary Chromosomes to Gene Expansion

The ascomycetous fungus Nectria haematococca, (asexual name Fusarium solani), is a member of a group of >50 species known as the “Fusarium solani species complex”. Members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. The current research analyzed the most extensively studied member of this complex, N. haematococca mating population VI (MPVI). Several genes controlling the ability of individual isolates of this species to colonize specific habitats are located on supernumerary chromosomes. Optical mapping revealed that the sequenced isolate has 17 chromosomes ranging from 530 kb to 6.52 Mb and that the physical size of the genome, 54.43 Mb, and the number of predicted genes, 15,707, are among the largest reported for ascomycetes. Two classes of genes have contributed to gene expansion: specific genes that are not found in other fungi including its closest sequenced relative, Fusarium graminearum; and genes that commonly occur as single copies in other fungi but are present as multiple copies in N. haematococca MPVI. Some of these additional genes appear to have resulted from gene duplication events, while others may have been acquired through horizontal gene transfer. The supernumerary nature of three chromosomes, 14, 15, and 17, was confirmed by their absence in pulsed field gel electrophoresis experiments of some isolates and by demonstrating that these isolates lacked chromosome-specific sequences found on the ends of these chromosomes. These supernumerary chromosomes contain more repeat sequences, are enriched in unique and duplicated genes, and have a lower G+C content in comparison to the other chromosomes. Although the origin(s) of the extra genes and the supernumerary chromosomes is not known, the gene expansion and its large genome size are consistent with this species' diverse range of habitats. Furthermore, the presence of unique genes on supernumerary chromosomes might account for individual isolates having different environmental niches

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Análisis de los hurtos en Colombia durante el año 2017 mediante los modelos de regresión lineal múltiple y la regresión ponderada geográficamente

Abstract According to information from the crime observatory of the National Police of Colombia, thefts from people and of cell phones have shown an upward trend since 2003 (Norza, Peñalosa and Rodríguez, 2017). This trend motivated the carrying out of the current study to analyze the relationship between socioeconomic factors and theft in the different municipalities of Colombia during 2017, through the use of multiple linear regression models and geographically weighted regression using secondary information sources segregated to municipal level. It was validated that variables enrolled in higher education institutions per thousand people, budget per capita allocated by the general system of participations and the category of the municipality account for 69,5% of the variability of the logarithm of theft from individuals and of cellphones in 532 municipalities using a globally estimated multiple linear regression model and 50,16% using the geographically weighted regression model omitting in the latter the category of the municipality. In this model there were slight variations in the coefficients at the municipal level, reflecting that the social and economic heterogeneity has effects on indicators of theft nationwide.Resumo Segundo informação proveniente do observatório do delito da Polícia Nacional da Colômbia, os furtos a pessoas e de celulares têm apresentado uma tendência de aumento desde o ano 2003 (Norza, Peñalosa y Rodríguez, 2017). Esta tendência motivou a realização do presente estudo para analisar a relação entre os fatores socioeconómicos e o furto em os diferentes municípios da Colômbia durante o ano 2017, mediante o uso de modelos de regressão linear múltipla e regressão geograficamente ponderada utilizando fontes de informação secundária segregada a nível municipal. Constatou-se que as variáveis matriculadas em instituições de ensino superior por cada mil pessoas, orçamento per capita atribuído pelo sistema geral de participações e a categoria do município explicam o 69,5% da variabilidade do logaritmo do furto a pessoas e de celulares em 532 municípios mediante um modelo de regressão linear múltipla estimado globalmente e o 50,16% utilizando o modelo de regressão ponderada geograficamente omitindo neste último a categoria do município. Neste modelo houve ligeiras variações nos coeficientes a nível municipal, o que reflete que a heterogeneidade social e económica tem efeitos nos indicadores de furto a nível nacional.Resumen Según información procedente del observatorio del delito de la Policía Nacional de Colombia, los hurtos a personas y de celulares han presentado una tendencia al alza desde el año 2003 (Norza, Peñalosa y Rodríguez, 2017). Esta tendencia motivó la realización del presente estudio para analizar la relación entre los factores socioeconómicos y el hurto en los diferentes municipios de Colombia durante el año 2017, mediante el uso de modelos de regresión lineal múltiple y regresión geográficamente ponderada utilizando fuentes de información secundaria segregada a nivel municipal. Se constató que las variables matriculados en instituciones de educación superior por cada mil personas, presupuesto per cápita asignado por el sistema general de participaciones y la categoría del municipio explican el 69,5% de la variabilidad del logaritmo del hurto a personas y de celulares en 532 municipios mediante un modelo de regresión lineal múltiple estimado globalmente y el 50,16% utilizando el modelo de regresión ponderada geográficamente omitiendo en este último la categoría del municipio. En este modelo hubo ligeras variaciones en los coeficientes a nivel municipal, lo que refleja que la heterogeneidad social y económica tiene efectos en los indicadores de hurto a nivel nacional

Intersectin 1 contributes to phenotypes in vivo: implications for Down Syndrome

Intersectin 1 (ITSN1) is a chromosome 21 (HSA21) gene product encoding a multidomain scaffold protein that functions in endocytosis, signal transduction and is implicated in Down Syndrome, Alzheimer’s Disease, and potentially other neurodegenerative diseases through activation of c-Jun N-terminal kinase (JNK). We report for the first time that ITSN1 proteins are elevated in Down Syndrome individuals of varying ages. However, ITSN1 levels decreased in aged Down Syndrome cases with Alzheimer’s Disease-like neuropathology. Analysis of a novel ITSN1 transgenic mouse reveals that ITSN1 overexpression results in a sex-dependent decrease in locomotor activity. This study reveals a link between overexpression of specific ITSN1 isoforms and behavioral phenotypes and has implications for human neurodegenerative diseases such as Down Syndrome and Alzheimer’s Disease

Determination of bone maturation according to the cervical vertebrae morphology, sex, and facial biotype of children and adolescents from Córdoba, Argentina

Fil: Damiani, Patricia María. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra integral niños y adolescentes "A"; Argentina.Fil: Rugani, Marta Leonor. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra integral niños y adolescentes A; Argentina.Fil: Rubial, María Cristina. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Rugani, Nelson Livio Jesús. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Prostodoncia IV B; Argentina.Fil: Pavani Carrillo, Jorge José. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Ortodoncia A; Argentina.Fil: Albornoz, Cristina Inés. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Goldenberg, Rosa Mariela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Melano, Marianela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Calvimonte, Cesar Andrés. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Diagnóstico por Imágenes A; Argentina.La predicción del crecimiento del complejo cráneo facial nos permite conocer el grado de maduración ósea con el fin de determinar un plan de tratamiento acorde al período en que se encuentra el paciente y elegir la terapéutica adecuada: ortopedia, ortodoncia, y/o cirugía ortognática. Existen estudios sobre vértebras cervicales que permiten valorar el grado de maduración ósea a través de la telerradiografía lateral de cráneo, aunque no se han encontrado investigaciones con estas características en nuestra población.Hipótesis: correlación entre la morfología de las vertebras cervicales y el pico máximo de crecimiento. Objetivo general: minimizar el grado de radiación a la que se expone niños y adolescentes cuando se pretende determinar la maduración osea, mediante la morfología de las vertebras cervicales a través de la telerradiografía rutinariamente evaluada, obviando el uso de la radiografía carpal.During the process of growth and development, a series of events occur with more or less regularity and similarity in all children from birth through adulthood. Carpal X-ray showed a large number of secondary ossification centers, considered “Indicators of maturity”, located in the hand, wrist, and distal epiphyses of the ulna and radius. Currently, morphological changes in the cervical vertebrae are considered indicators of bone maturation. Objective: in order to minimize radiation in children and adolescents when determining the degree of bone maturation, carpal radiography was replaced by lateral tele radiography of the skull, which is normally used, also checking the age of maturation in our population. When deciding the treatment plan, this study allowed us to determine the method to use for the resolution of the clinical case: orthopedics, orthodontics, or both at the same time. Materials and methods: a cross-sectional study without patient follow-up. Latera X-rays of the skull, orthopantomography and carpus were analyzed from 318 children and adolescents of both sexes aged 10 to16 years of age with permanent teeth in both dental arches, with/without the presence of the 2nd molar. The facial biotype was determined by the Björk-Jarabak cephalogram. Results: there were no significant variations between the mean ages in the different facial biotypes, where lower mean values were observed in males with a dolichofacial biotype and meso biotype with a tendency to brachyfacial. In girls, it was observed that most of them were significantly related (p<0-05) with the exception of mesofacial biotype between chronological ages with vertebral bone ages and vertebral bone ages with carpal and dental bones. While in males, there was an exception in the dolichofacial biotype in all variables, being only significant among the variables: chronological ages with carpal bones and vertebral ages with carpal bones. Therefore, we can conclude that there is a high correlation between vertebral, carpal, and dental bone ages in both sexes and facial biotypes, except in girls with mesofacial biotype.Fil: Damiani, Patricia María. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra integral niños y adolescentes "A"; Argentina.Fil: Rugani, Marta Leonor. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra integral niños y adolescentes A; Argentina.Fil: Rubial, María Cristina. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Rugani, Nelson Livio Jesús. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Prostodoncia IV B; Argentina.Fil: Pavani Carrillo, Jorge José. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Ortodoncia A; Argentina.Fil: Albornoz, Cristina Inés. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Goldenberg, Rosa Mariela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Melano, Marianela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Ortodoncía A; Argentina.Fil: Calvimonte, Cesar Andrés. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Diagnóstico por Imágenes A; Argentina.Otras Ciencias de la Salu