Implementation and validation of an extended Schnerr-Sauer cavitation model for non-isothermal flows in OpenFOAM

Abstract In the present work cavitation in liquid hydrogen and nitrogen was investigated by using the open source toolbox OpenFOAM. Simulations were performed by means of a mass transfer model, based on the homogeneous mixture approach in combination with the Volume of Fluid (VOF) method for the reconstruction the liquid-vapor interface. Two additional transport equations were considered, i.e. the liquid volume fraction advection and the temperature equation. The implementation of an extended Schnerr- Sauer model allowed for the introduction of the thermal effects in terms of latent heat release/absorption and convective heat transfer inside the liquid-vapor interface. A set of Antoine-like equations relate the saturation conditions to the local conditions

Mode decomposition methods for the analysis of cavitating flows in turbomachinery

Abstract The present work is aimed at the characterization of the cavitating flow regimes by applying the coupled POD/DMD technique to the vapor volume fraction field. The proposed approach provided an improved spatio-temporal-frequency description of the flow, based on the detection of the most energetic flow structures with information about their shape and size, and their decomposition into wave patterns oscillating with specific frequency and decay rate. The novel technique was applied to numerical results concerning the bubble cavitation and the supercavitation regimes of 2D water flows around a NACA hydrofoil at ambient temperature. Numerical simulations were performed by using a homogenous mixture model equipped with an extended Schnerr-Sauer cavitation model, in combination with a Volume of Fluid (VOF) interface tracking method. The proposed approached provided a better characterization of the unsteady cavitating flow, and allowed for a deeper insight about the dynamics of the vapor cavity, especially in cases involving the more chaotic regime of supercavitation. In particular, POD results figured out the most energetic coherent vapor structures associated to each cavitation regime: the first mode highlighted the main sheet cavity which grew on the hydrofoil up to detached, the second mode pointed out the cavitating/condensating doublet structures and the third mode figured out the smaller structures owning less energy but a higher frequency content. DMD modes performed a decomposition of the coherent structures detected by means of the POD analysis, into a subset of vapor pattern periodically evolving with a single frequency and a characteristic decay rate. Furthermore, results showed that the supercavitating flow structures owned characteristic frequencies which ranged from 5 to 26 Hz, while the less intensive bubble cavitation regime was characterized by frequencies ranging from 15 to 42 Hz

Modeling viscous effects on boundary layer of rarefied gas flows inside micronozzles in the slip regime condition

Abstract The present work provided a numerical investigation of the supersonic flow of rarefied gas into a planar micronozzle characterized by small depth and long divergent section. 2D and 3D computational fluid dynamics (CFD) computations were performed using the continuum Navier-Stokes equations in combination with partial slip conditions at walls, based on a the establishment of the slip regime related to a Knudsen number ranging between 1 x 10-3 and 1 x 10-1. Different partial slip conditions were considered, i.e. the ideal case of pure slip conditions and the full viscous case with Maxwellian slip conditions on sidewalls and planar walls, as well as the case of Maxwellian slip just on sidewalls. The Maxwell slip model was set with a tangential accommodation coefficient equal (TMAC) to 0.8. Comparisons were based on the estimation of the global performance of the micronozzle in terms of thrust force, specific impulse, discharge coefficient and Isp-efficiency. It resulted that when the nozzle depth was neglected, 3D simulations led to the same solution obtained by means of 2D computations inside the micronozzle. The boundary layer thicknesses experienced a linear growth on the sidewalls, and the viscous losses produced a reduction of the performance of about the 95%. Significant differences were found in the prediction of the jet plume, which took the typical bell-shape form in cases involving 2D computations, yet 3D simulations estimated a plume characterized by the succession of oblique shock waves and expansion fan waves. Instead, when the nozzle depth was considered, 3D simulations underlined a completely different behavior of the flow because of the establishment of the nozzle blockage and a viscous heating. The performance suffered an intense degradation of about the 47%, and the analysis of the jet plume highlighted the formation of the Mach disk followed by the typical diamond-shaped subsonic recirculation region

Mems vaporazing liquid microthruster: A comprehensive review

none4The interest in developing efficient nano and pico-satellites has grown in the last 20 years. Secondary propulsion systems capable of serving specific maneuvers are an essential part of these small satellites. In particular, Micro-Electro-Mechanical Systems (MEMS) Vaporizing Liquid Micro-thrusters (VLM), using water as a propellant, represent today a smart choice in terms of simplicity and cost. In this paper, we first propose a review of the international literature focused on MEMS VLM development, reviewing the different geometries and heating solutions proposed in the liter-ature. Then, we focus on a critical aspect of these micro thrusters: the presence of unstable phenom-ena. In particular, the boiling instabilities and reverse channel flow substantially impact the MEMS VLMs’ performance and limit their applicability. Finally, we review the research focused on the passive and active control of the boiling instabilities, based on VLM geometry optimization and active heating strategies, respectively. Today, these ones represent the two principal research axes followed by the scientific community to mitigate the drawbacks linked to the use of MEMS VLMs.openFontanarosa D.; Francioso L.; De Giorgi M.G.; Vetrano M.R.Fontanarosa, D.; Francioso, L.; De Giorgi, M. G.; Vetrano, M. R

Expression profiling of miRNA-145 and miRNA-338 in serum and sputum of patients with COPD, asthma, and asthmaâCOPD overlap syndrome phenotype

Background and objectives: A new phenotype with overlapping characteristics between asthma and chronic obstructive pulmonary disease (COPD) called asthmaâCOPD overlap syndrome (ACOS) is emerging among inflammation diseases. To date, there is no agreement on specific criteria to define this syndrome, and the current guidelines are insufficient to classify the analogy and differences between overlap and COPD or asthma phenotypes. It would be necessary to identify new biomarkers able to identify these diseases clearly. Thus, the aim of this study was to identify a serum and supernatant of sputum microRNA (miRNA) expression profile of miRNA-145 and miRNA-338 in patients with asthma (n=13), COPD (n=31), and ACOS (n=8) and controls (n=7). Methods: The expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). For statistical analysis, the ANOVA test, KruskalâWallis test, MannâWhitney U-test, and Spearmanâs rank correlation were used. Results: The main finding of this work is that the expression of miRNA-338 is higher in the supernatant of different obstructive diseases than in peripheral blood, while miRNA-145 is higher only in the supernatant of asthma patients. The expression of both selected miRNAs is higher in the supernatant of asthma and COPD patients than in controls. Conclusion: Differences in sputum miRNA expression profile were observed between patients with ACOS and asthma or COPD, which underline the potential role of miRNA as a biomarker that is able to discriminate patients with ACOS, asthma, and COPD

Numerical investigation of the performance of Contra-Rotating Propellers for a Remotely Piloted Aerial Vehicle

Abstract The present work aims at the numerical prediction of the performance of a Contra-Rotating Propellers (CRP) system for a Remotely Piloted Aerial Vehicles (RPAV). The CRP system was compared with an equivalent counter-rotating propellers configuration which was set by considering two eccentric propellers which were rotating at the same speed. Each contra-rotating test case was built by varying the pitch angle of blades of the rear propeller, while the front propeller preserved the original reconstructed geometry. Several pitch configurations and angular velocities of the rear propeller was simulated. Comparisons showed an improvement of the propulsive efficiency of the contra-rotating configuration in case of larger pitch angles combined with slower angular velocities of the rear propeller

The emerging role of the RBM20 and PTBP1 ribonucleoproteins in heart development and cardiovascular diseases

Alternative splicing is a regulatory mechanism essential for cell differentiation and tissue organization. More than 90% of human genes are regulated by alternative splicing events, which participate in cell fate determination. The general mechanisms of splicing events are well known, whereas only recently have deep-sequencing, high throughput analyses and animal models provided novel information on the network of functionally coordinated, tissue-specific, alternatively spliced exons. Heart development and cardiac tissue differentiation require thoroughly regulated splicing events. The ribonucleoprotein RBM20 is a key regulator of the alternative splicing events required for functional and structural heart properties, such as the expression of TTN isoforms. Recently, the polypyrimidine tract-binding protein PTBP1 has been demonstrated to participate with RBM20 in regulating splicing events. In this review, we summarize the updated knowledge relative to RBM20 and PTBP1 structure and molecular function; their role in alternative splicing mechanisms involved in the heart development and function; RBM20 mutations associated with idiopathic dilated cardiovascular disease (DCM); and the consequences of RBM20-altered expression or dysfunction. Furthermore, we discuss the possible application of targeting RBM20 in new approaches in heart therapies

Molecular and lifestyle factors modulating obesity disease

Obesity adversely affects bone health by means of multiple mechanisms, e.g., alterations in bone-regulating hormones, inflammation, and oxidative stress. Substantial evidence supports the relationship between adiposity and bone disorders in overweight/obese individuals. It is well known that the balance between mutually exclusive differentiation of progenitor cells into osteoblasts or adipocytes is controlled by different agents, including growth factors, hormones, genetic and epigenetic factors. Furthermore, an association between vitamin D deficiency and obesity has been reported. On the other hand, regular physical activity plays a key role in weight control, in the reduction of obesity-associated risks and promotes osteogenesis. The aim of this review is to highlight relevant cellular and molecular aspects for over-weight containment. In this context, the modulation of progenitor cells during differentiation as well as the role of epigenetics and microbiota in obesity disease will be discussed. Furthermore, lifestyle changes including an optimized diet as well as targeted physical activity will be suggested as strategies for the treatment of obesity disease

Signalment, Immunological and Parasitological Status and Clinicopathological Findings of Leishmania-Seropositive Apparently Healthy Dogs

Canine leishmaniosis caused by Leishmania infantum is a disease with a wide range of clinical manifestations. Epidemiological serosurveys performed in Europe often lack a thorough assessment of clinical health status of studied dogs. The aim of this study was to evaluate signalment, immunological and parasitological status and clinicopathological findings of L. infantum-seropositive apparently healthy dogs (n = 212) living in endemic areas. Routine laboratory tests, endpoint inhouse ELISA to quantify the anti-Leishmania antibodies, blood Leishmania qPCR and IFN-ELISA were performed. All dogs enrolled were L. infantum-seropositive and were classified as healthy (n = 105) or sick (n = 107) according to LeishVet guidelines. The sick group presented a higher proportion of medium to high antibody levels and positive qPCR and lower IFN-concentration compared to the healthy group. Sick dogs were mostly classified in LeishVet stage IIa. Biochemical alterations (98%) were the most common clinicopathological findings, with fewer urinary tract (46%) and hematological (40%) alterations. Apparently healthy L. infantum-seropositive dogs can be classified between truly healthy dogs and sick dogs with clinicopathological findings. Sick dogs presented medium to high seropositivity and parasitemia and low IFN- concentrations, and their most common clinicopathological abnormalities were serum protein alterations followed by proteinuria and lymphopenia

Advanced cellular models for rare disease study: exploring neural, muscle and skeletal organoids

Organoids are self-organized, three-dimensional structures derived from stem cells that can mimic the structure and physiology of human organs. Patient-specific induced pluripotent stem cells (iPSCs) and 3D organoid model systems allow cells to be analyzed in a controlled environment to simulate the characteristics of a given disease by modeling the underlying pathophysiology. The recent development of 3D cell models has offered the scientific community an exceptionally valuable tool in the study of rare diseases, overcoming the limited availability of biological samples and the limitations of animal models. This review provides an overview of iPSC models and genetic engineering techniques used to develop organoids. In particular, some of the models applied to the study of rare neuronal, muscular and skeletal diseases are described. Furthermore, the limitations and potential of developing new therapeutic approaches are discussed