Hierarchical factor structure of the Intolerance of Uncertainty Scale short form (IUS-12) in the Italian version

Despite widespread use, few translations are available for the Intolerance of Uncertainty Scale short form (IUS-12) as well as limited research on its psychometric properties in Italy. Moreover, recent evidence has suggested a multifaceted hierarchical structure for this scale. We compared the two-factor model to second-order and bi-factor models, in which a General IU factor was posited with two more narrow factors: Prospective IU and Inhibitory IU. Models were tested on a pooled dataset of students (N = 609) taking the IUS-12 alone or with other IUS-27 items. The bi-factor model fitted the sample data better than alternative models. The general factor accounted for 80% of the item variance. Presentation mode did not impact scalar invariance. Convergent validity with neuroticism, need for closure, and the uncertainty response scale was high for the total score. As such, scoring the IUS-12 total score is recommended in clinical research and assessmen

Applications of Engineering 4.0 to Improve the Safety of Metalworking Operators: The Ansaldo Energia Case

The paper describes how, on behalf of Ansaldo Energia Spa, a multidisciplinary team developed a methodology based on Industry 4.0 technologies, an approach that allows rescue teams to quickly intervene in the event of a mandown in isolated areas of the plant, where the unfortunate person would risk being found with significant delay and onsequent problems for his physical well-being. Under the supervision of the team, a highly specialized supplier created a suitable hardware and software device to achieve this outcome. Such a device can immediately warn rescue crews in real time as soon as an incident occurs, as well as geo-locate the man on the ground with exceptional precision. Once developed, the approach was standardized in a set of sequential and generic procedures in order to make it adaptable to any sort of firm, construction site, or workshop where a man-down event may happen. The methodology is set up as a real toolkit to protect operators from severe damage that can result from long waits for rescue teams, whenever operators experience negative events for their safety being them exogenous (fainting illnesses, heart attacks, epileptic attacks, strokes, etc.) or endogenous (accidents in the workplace)

Proline synthesis in developing microspores is required for pollen development and fertility

Background: In many plants, the amino acid proline is strongly accumulated in pollen and disruption of proline synthesis caused abortion of microspore development in Arabidopsis. So far, it was unclear whether local biosynthesis or transport of proline determines the success of fertile pollen development. Results: We analyzed the expression pattern of the proline biosynthetic genes PYRROLINE-5-CARBOXYLATE SYNTHETASE 1 & 2 (P5CS1 & 2) in Arabidopsis anthers and both isoforms were strongly expressed in developing microspores and pollen grains but only inconsistently in surrounding sporophytic tissues. We introduced in a p5cs1/p5cs1 p5cs2/P5CS2 mutant background an additional copy of P5CS2 under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter, the tapetum-specific LIPID TRANSFER PROTEIN 12 (Ltp12) promoter or the pollen-specific At5g17340 promoter to determine in which site proline biosynthesis can restore the fertility of proline-deficient microspores. The specificity of these promoters was confirmed by β-glucuronidase (GUS) analysis, and by direct proline measurement in pollen grains and stage-9/10 anthers. Expression of P5CS2 under control of the At5g17340 promoter fully rescued proline content and normal morphology and fertility of mutant pollen. In contrast, expression of P5CS2 driven by either the Ltp12 or CaMV35S promoter caused only partial restoration of pollen development with little effect on pollen fertility. Conclusions: Overall, our results indicate that proline transport is not able to fulfill the demand of the cells of the male germ line. Pollen development and fertility depend on local proline biosynthesis during late stages of microspore development and in mature pollen grains

RSM approach for stochastic sensitivity analysis of the economicsustainability of a methanol production plant using renewable energy sources

This study aims at investigating the economic viability, at the pre-feasibility level, of a 5 MW electrolyserbase-methanol production plant, coupled with a PV power plant. The Authors investigated the impact ofdifferent parameters, such as the PV plant size, the electrical energy cost and the components capitalcosts on the methanol production cost and on the system economic viability. It was also analyzed theminimum recommended sale price of the methanol in order to assure an adequate time frame for thereturn of the investment, considering a different combination of the investigated parameters.An economic sensitivity analysis, based on the RSM approach, was performed in order to define themost promising economic conditions under which the plant can be considered a profitable investment interms of ARR. A guide for an economically viable plant design, allowing for the identification of the mostsuitable combination of the economic parameters, was proposed as a kind of\u201cmaps of existence\u201d. For thereference case, the Methanol Production Cost (MPC) resulted around 324V/ton and the minimummethanol sale price to achieve a PBP of 10 years. The sensitivity analysis identified the cost of electricityand the capital cost of the electrolyser as the most affecting parameters for the system economic viability.In terms of ARR, the methanol price represents the most significant factor. Considering a methanol saleprice ranging between 400 and 1200eur/ton, the ARR varied from 5% (20 year of PBP) to 20% (5years ofPBP). From the environmental point of view, it is worth underling that the methanol production planthere proposed allows to recycle about 5800 tons of CO2per year and to avoid the consumption of about5.2 MNm3of NG per year (compared to the traditional production)

Erythrocyte's aging in microgravity highlights how environmental stimuli shape metabolism and morphology

The determination of the function of cells in zero-gravity conditions is a subject of interest in many different research fields. Due to their metabolic unicity, the characterization of the behaviour of erythrocytes maintained in prolonged microgravity conditions is of particular importance. Here, we used a 3D-clinostat to assess the microgravity-induced modifications of the structure and function of these cells, by investigating how they translate these peculiar mechanical stimuli into modifications, with potential clinical interest, of the biochemical pathways and the aging processes. We compared the erythrocyte's structural parameters and selected metabolic indicators that are characteristic of the aging in microgravity and standard static incubation conditions. The results suggest that, at first, human erythrocytes react to external stimuli by adapting their metabolic patterns and the rate of consumption of the cell resources. On longer timeframes, the cells translate even small differences in the environment mechanical solicitations into structural and morphologic features, leading to distinctive morphological patterns of agin

Muscle activations during functional tasks in individuals with chronic ankle instability: a systematic review of electromyographical studies

Background: It has been reported that individuals with chronic ankle instability (CAI) show motor control ab-normalities. The study of muscle activations by means of surface electromyography (sEMG) plays a key role in understanding some of the features of movement abnormalities. Research question: Do common sEMG activation abnormalities and strategies exists across different functional movements? Methods: Literature review was conducted on PubMed, Web-of-Science and Cochrane databases. Studies pub-lished between 2000 and 2020 that assessed muscle activations by means of sEMG during any type of functional task in individuals with CAI, and used healthy individuals as controls, were included. Methodological quality was assessed using the modified Downs&Black checklist. Since the methodologies of different studies were hetero-geneous, no meta-analysis was conducted. Results: A total of 63 articles investigating muscle activations during gait, running, responses to perturbations, landing and hopping, cutting and turning; single-limb stance, star excursion balance task, forward lunges, ball- kicking, y-balance test and single-limb squatting were considered. Individuals with CAI showed a delayed activation of the peroneus longus in response to sudden inversion perturbations, in transitions between double- and single-limb stance, and in landing on unstable surfaces. Apparently, while walking on ground there are no differences between CAI and controls, walking on a treadmill increases the variability of muscles activations, probably as a “safety strategy” to avoid ankle inversion. An abnormal activation of the tibialis anterior was observed during a number of tasks. Finally, hip/spine muscles were activated before ankle muscles in CAI compared to controls. Conclusion: Though the methodology of the studies herein considered is heterogeneous, this review shows that the peroneal and tibialis anterior muscles have an abnormal activation in CAI individuals. These individuals also show a proximal muscle activation strategy during the performance of balance challenging tasks. Future studies should investigate whole-body muscle activation abnormalities in CAI individuals

Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles

We exploited a variety of mouse models to assess the roles of JP45-CASQ1 (CASQ, calsequestrin) and JP45-CASQ2 on calcium entry in slow twitch muscles. In flexor digitorum brevis (FDB) fibers isolated from JP45-CASQ1-CASQ2 triple KO mice, calcium transients induced by tetanic stimulation rely on calcium entry via La3+- and nifedipine-sensitive calcium channels. The comparison of excitation-coupled calcium entry (ECCE) between FDB fibers from WT, JP45KO, CASQ1KO, CASQ2KO, JP45-CASQ1 double KO, JP45-CASQ2 double KO, and JP45-CASQ1-CASQ2 triple KO shows that ECCE enhancement requires ablation of both CASQs and JP45. Calcium entry activated by ablation of both JP45-CASQ1 and JP45-CASQ2 complexes supports tetanic force development in slow twitch soleus muscles. In addition, we show that CASQs interact with JP45 at Ca2+ concentrations similar to those present in the lumen of the sarcoplasmic reticulum at rest, whereas Ca2+ concentrations similar to those present in the SR lumen after depolarization-induced calcium release cause the dissociation of JP45 from CASQs. Our results show that the complex JP45-CASQs is a negative regulator of ECCE and that tetanic force development in slow twitch muscles is supported by the dynamic interaction between JP45 and CASQs

A correlational analysis of the relationships among intolerance of uncertainty, anxiety sensitivity, subjective sleep quality, and insomnia symptoms

In this study, we used structural equation modeling to investigate the interplay among Intolerance of Uncertainty (IU), Anxiety Sensitivity (AS), and sleep problems. Three hundred undergraduate students completed the Intolerance of Uncertainty Scale, the Intolerance of Uncertainty Inventory, the Anxiety Sensitivity Index, the Beck Depression Inventory, the State-Trait Anxiety Inventory, the Pittsburgh Sleep Quality Index and the Insomnia Severity Index. 68% and 40% of the students reported poor sleep quality or sub-threshold insomnia problems, respectively. Depression and anxiety levels were above the cut-off for about one-fourth of the participants. Structural equation modeling revealed that IU was strongly associated with AS, in turn influencing both insomnia severity and sleep quality via depression and anxiety. Significant indirect effects revealed that an anxious pathway was more strongly associated with insomnia severity, while a depression pathway was more relevant for worsening the quality of sleep. We discussed the results in the frameworks of cognitive models of insomnia. Viewing AS and IU as antecedents of sleep problems and assigning to AS a pivotal role, our study suggested indications for clinical interventions on a population at risk for sleep disorders