Comorbidities of patients in tiotropium clinical trials : comparison with observational studies of patients with chronic obstructive pulmonary disease

Acknowledgments The authors are fully responsible for all content and editorial decisions made, were involved at all stages of manuscript development, and have approved the final version for publication. Editorial assistance, supported financially by Boehringer Ingelheim and Pfizer, was provided by Godfrey Lisk of PAREXEL International during the preparation of this manuscript. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited.Peer reviewedPublisher PD

Slow Synaptic Inhibition in Nucleus HVc of the Adult Zebra Finch

Nervous systems process information over a broad range of time scales and thus need corresponding cellular mechanisms spanning that range. In the avian song system, long integration times are likely necessary to process auditory feedback of the bird’s own vocalizations. For example, in nucleus HVc, a center that contains both auditory and premotor neurons and that is thought to act as a gateway for auditory information into the song system, slow inhibitory mechanisms appear to play an important role in the processing of auditory information. These long-lasting processes include inhibitory potentials thought to shape auditory selectivity and a vocalization-induced inhibition of auditory responses lasting several seconds. To investigate the possible cellular mechanisms of these long-lasting inhibitory processes, we have made intracellular recordings from HVc neurons in slices of adult zebra finch brains and have stimulated extracellularly within HVc. A brief, high-frequency train of stimuli (50 pulses at 100 Hz) could elicit a hyperpolarizing response that lasted 2–20 sec. The slow hyperpolarization (SH) could still be elicited in the presence of glutamate receptor blockers, suggesting that it does not require polysynaptic excitation. Three major components contribute to this activity-induced SH: a long-lasting GABA_B receptor-mediated IPSP, a slow afterhyperpolarization requiring action potentials but not Ca²⁺ influx, and a long-lasting IPSP, the neurotransmitter and receptor of which remain unidentified. These three slow hyperpolarizing events are well placed to contribute to the observed inhibition of HVc neurons after singing and could shape auditory feedback during song learning

Исследования функциональности и состояния защитных сооружений ГО г. Юрги

Immunogenetics and cellular immunology of bacterial infectious disease

Разработка и исследование асинхронного электропривода с наблюдателем состояния

Выпускная квалификационная работа 109 с., 35 рис., 18 табл., 47 источников, 5 прил. Объектом исследования является дискретная математическая модель наблюдателя состояния полного порядка асинхронного двигателя. Цель работы – Разработка и исследование асинхронного электропривода с наблюдателем состояния В процессе исследования проводилось имитационное моделирование разработанной дискретной математической модели асинхронного двигателя и разработанной дискретной математической модели наблюдателя состояния полного порядкаFinal qualifying work 109 p., 35 fig., 18 tab., 47 sources, 5 adj. The object of research is a discrete mathematical model of the observer status of full order of the induction motor. Objective - Development and research of the asynchronous electric drive with observer status The study was conducted simulations developed discrete mathematical model of the induction motor and the developed mathematical model of discrete observer of full order stat

The Search for Low-mass Companions of B Stars in the Carina Nebula Cluster Trumpler 16

We have developed lists of likely B3--A0 stars (called "late B" stars) in the young cluster Trumpler 16. The following criteria were used: location within 3' of Eta Car, an appropriate V and B-V combination, and proper motion (where available). Color and magnitude cuts have been made assuming an E(B-V) =0.55 mag +/- 0.1, which is a good approximation close to the center of Trumpler 16. These lists have been cross-correlated with X-ray sources found in the Chandra Carina Complex Project (CCCP). Previous studies have shown that only very rarely (if at all) do late main sequence B stars produce X-rays. We present evidence that the X-ray detected sources are binaries with low-mass companions, since stars less massive than 1.4 Msun are strong X-ray sources at the age of the cluster. Both the median X-ray energies and X-ray luminosities of these sources are in good agreement with values for typical low-mass coronal X-ray sources. We find that 39% of the late B stars based on a list with proper motions have low-mass companions. Similarly, 32% of a sample without proper motions have low-mass companions. We discuss the X-ray detection completeness. These results on low-mass companions of intermediate mass stars are complementary to spectroscopic and interferometric results, and probe new parameter space of low mass companions at all separations. They do not support a steeply rising distribution of mass ratios to low masses for intermediate-mass (5 Msun) primaries, such as would be found by random pairing from the Initial Mass Function.Comment: Accepted for the ApJS Special Issue on the Chandra Carina Complex Project (CCCP), scheduled for publication in May 2011. All 16 CCCP Special Issue papers are available at http://cochise.astro.psu.edu/Carina_public/special_issue.html through 2011 at leas

Using Both Sides of Your Brain: The Case for Rapid Interhemispheric Switching

Individual brain hemispheres are often specialized for specific aspects of a behavior. How both sides of the brain coordinate their output to produce a perfectly seamless behavior is not known. Songbirds appear to achieve this by rapidly switching back and forth between hemispheres

Resonance fluorescence of a trapped three-level atom

We investigate theoretically the spectrum of resonance fluorescence of a harmonically trapped atom, whose internal transitions are $\Lambda$--shaped and driven at two-photon resonance by a pair of lasers, which cool the center--of--mass motion. For this configuration, photons are scattered only due to the mechanical effects of the quantum interaction between light and atom. We study the spectrum of emission in the final stage of laser--cooling, when the atomic center-of-mass dynamics is quantum mechanical and the size of the wave packet is much smaller than the laser wavelength (Lamb--Dicke limit). We use the spectral decomposition of the Liouville operator of the master equation for the atomic density matrix and apply second order perturbation theory. We find that the spectrum of resonance fluorescence is composed by two narrow sidebands -- the Stokes and anti-Stokes components of the scattered light -- while all other signals are in general orders of magnitude smaller. For very low temperatures, however, the Mollow--type inelastic component of the spectrum becomes visible. This exhibits novel features which allow further insight into the quantum dynamics of the system. We provide a physical model that interprets our results and discuss how one can recover temperature and cooling rate of the atom from the spectrum. The behaviour of the considered system is compared with the resonance fluorescence of a trapped atom whose internal transition consists of two-levels.Comment: 11 pages, 4 Figure

Loss of Roquin induces early death and immune deregulation but not autoimmunity

Deletion of Roquin in T or B cells, or in the entire hematopoietic system of mice, alters immune homeostasis but does not result in autoimmunity

Plant-RRBS, a bisulfite and next-generation sequencing-based methylome profiling method enriching for coverage of cytosine positions

Background: Cytosine methylation in plant genomes is important for the regulation of gene transcription and transposon activity. Genome-wide methylomes are studied upon mutation of the DNA methyltransferases, adaptation to environmental stresses or during development. However, from basic biology to breeding programs, there is a need to monitor multiple samples to determine transgenerational methylation inheritance or differential cytosine methylation. Methylome data obtained by sodium hydrogen sulfite (bisulfite)-conversion and next-generation sequencing (NGS) provide genome- wide information on cytosine methylation. However, a profiling method that detects cytosine methylation state dispersed over the genome would allow high-throughput analysis of multiple plant samples with distinct epigenetic signatures. We use specific restriction endonucleases to enrich for cytosine coverage in a bisulfite and NGS-based profiling method, which was compared to whole-genome bisulfite sequencing of the same plant material. Methods: We established an effective methylome profiling method in plants, termed plant-reduced representation bisulfite sequencing (plant-RRBS), using optimized double restriction endonuclease digestion, fragment end repair, adapter ligation, followed by bisulfite conversion, PCR amplification and NGS. We report a performant laboratory protocol and a straightforward bioinformatics data analysis pipeline for plant-RRBS, applicable for any reference-sequenced plant species. Results: As a proof of concept, methylome profiling was performed using an Oryza sativa ssp. indica pure breeding line and a derived epigenetically altered line (epiline). Plant-RRBS detects methylation levels at tens of millions of cytosine positions deduced from bisulfite conversion in multiple samples. To evaluate the method, the coverage of cytosine positions, the intra-line similarity and the differential cytosine methylation levels between the pure breeding line and the epiline were determined. Plant-RRBS reproducibly covers commonly up to one fourth of the cytosine positions in the rice genome when using MspI-DpnII within a group of five biological replicates of a line. The method predominantly detects cytosine methylation in putative promoter regions and not-annotated regions in rice. Conclusions: Plant-RRBS offers high-throughput and broad, genome- dispersed methylation detection by effective read number generation obtained from reproducibly covered genome fractions using optimized endonuclease combinations, facilitating comparative analyses of multi-sample studies for cytosine methylation and transgenerational stability in experimental material and plant breeding populations