Actividad antioxidante del gel a base de extracto de Origanum vulgare ¿Importante para la salud bucal? Estudio preliminar

Objective: To determine the antioxidant activity of the hydroalcoholic extract of Origanum vulgare through DPPH, ABTS and FRAP essays. Material and Methods: The sample collected from Origanum vulgare was dried, reduced in size and placed to macerate 500 grams of dry sample in 1000 mL of 97% ethanol for one week. After time, the macerate was processed through a filter and concentrated in an oven. The formulations were carried out and the dilutions were analyzed with the antioxidant essays. Results: DPPH Method, the 25% gel showed an IC50 of 98.485 mg / mL equivalent to the dilution of 78.789% and for the Trolox® standard, it presented IC50 of 2.48 µg / mL. ABTS Method, the 25% gel formulation presented IC50 of 3.687 mg / mL equivalent to a dilution of 77.75% and for the Trolox® standard it presented IC50 of 2.99 µg / mL, a difference from the other formulations. The results evidenced a relation between the percentage of inhibition and concentration of the samples with an accepted correlation (R2) for the gels at 25%, 50%, 75% of extract of Origanum vulgare and Trolox® of 0.9972; 0.9987 and 0.9986 respectively. FRAP Method, with fast action during the 4 minutes, being 125 mg of extract contained in the gel 25% equivalent to 4mg of Trolox®. Conclusions: It was determined that the antioxidant activity equivalent to Trolox®, with antioxidant assays, with the best average free radical uptake power (Trolox / mg) of extract was present in the gel of Extract of Origanum vulgare 25%.Objetivo: Determinar la actividad antioxidante del extracto hidroalcohólico de Origanum vulgare a través de 03 métodos de ensayo DPPH, ABTS y FRAP. Material y métodos: La muestra recolectada de Origanum vulgare se secó, redujo el tamaño y se colocó a macerar 500 gramos de muestra seca en 1000 mL de etanol 97% durante una semana. Pasado el tiempo se procedió a filtrar el macerado y se concentró en estufa. Se procedió a realizar las formulaciones. Estas diluciones, se sometieron a los análisis antioxidantes. Resultados: Método DPPH, el gel al 25% mostró un IC50 de 98,485 mg/mL equivalente a la dilución del 78,789% y para el caso del estándar Trolox® presentó un IC50 de 2,48 µg/mL. Método ABTS, la formulación de gel al 25% presentó un IC50 de 3,687 mg/mL equivalente a una dilución de 77,75% y para el estándar Trolox® presentó un IC50 de 2,99 µg/mL, a diferencia de las otras formulaciones. Se evidenció relación entre el porcentaje de inhibición y concentración de las muestras con una correlación aceptada (R2) para geles al 25%, 50%, 75% de extracto de Origanum vulgare y Trolox® de 0,9972; 0,9987 y 0,9986 respectivamente. Método FRAP, observó acción rápida durante los 4 minutos, siendo 125 mg de extracto contenido en el gel de 25% equivalente a 4mg de Trolox®. Conclusiones: Se determinó la actividad antioxidante equivalente al Trolox®, mediante análisis antioxidante, con mejor poder de captación de radical libre promedio (Trolox/mg) de extracto y estuvo presente en el gel a base de Extracto de Origanum vulgare al 25%

Caveolin-1-Enhanced Motility and Focal Adhesion Turnover Require Tyrosine-14 but Not Accumulation to the Rear in Metastatic Cancer Cells

Caveolin-1 is known to promote cell migration, and increased caveolin-1 expression is associated with tumor progression and metastasis. In fibroblasts, caveolin-1 polarization and phosphorylation of tyrosine-14 are essential to promote migration. However, the role of caveolin-1 in migration of metastatic cells remains poorly defined. Here, caveolin-1 participation in metastatic cell migration was evaluated by shRNA targeting of endogenous caveolin-1 in MDA-MB-231 human breast cancer cells and ectopic expression in B16-F10 mouse melanoma cells. Depletion of caveolin-1 in MDA-MB-231 cells reduced, while expression in B16-F10 cells promoted migration, polarization and focal adhesion turnover in a sequence of events that involved phosphorylation of tyrosine-14 and Rac-1 activation. In B16-F10 cells, expression of a non-phosphorylatable tyrosine-14 to phenylalanine mutant failed to recapitulate the effects observed with wild-type caveolin-1. Alternatively, treatment of MDA-MB-231 cells with the Src family kinase inhibitor PP2 reduced caveolin-1 phosphorylation on tyrosine-14 and cell migration. Surprisingly, unlike for fibroblasts, caveolin-1 polarization and re-localization to the trailing edge were not observed in migrating metastatic cells. Thus, expression and phosphorylation, but not polarization of caveolin-1 favor the highly mobile phenotype of metastatic cells

To Help or Not to Help? Prosocial Behavior, Its Association With Well-Being, and Predictors of Prosocial Behavior During the Coronavirus Disease Pandemic

Funding Information: This work was supported by grants from the Swiss National Science Foundation awarded to ATG (PP00P1_ 163716/1 and PP00P1_190082). The funder provided support in the form of salaries for authors (EH and ATG) but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: Copyright © 2022 Haller, Lubenko, Presti, Squatrito, Constantinou, Nicolaou, Papacostas, Aydın, Chong, Chien, Cheng, Ruiz, García-Martín, Obando-Posada, Segura-Vargas, Vasiliou, McHugh, Höfer, Baban, Dias Neto, da Silva, Monestès, Alvarez-Galvez, Paez-Blarrina, Montesinos, Valdivia-Salas, Ori, Kleszcz, Lappalainen, Ivanović, Gosar, Dionne, Merwin, Karekla, Kassianos and Gloster.The coronavirus disease (COVID-19) pandemic fundamentally disrupted humans’ social life and behavior. Public health measures may have inadvertently impacted how people care for each other. This study investigated prosocial behavior, its association well-being, and predictors of prosocial behavior during the first COVID-19 pandemic lockdown and sought to understand whether region-specific differences exist. Participants (N = 9,496) from eight regions clustering multiple countries around the world responded to a cross-sectional online-survey investigating the psychological consequences of the first upsurge of lockdowns in spring 2020. Prosocial behavior was reported to occur frequently. Multiple regression analyses showed that prosocial behavior was associated with better well-being consistently across regions. With regard to predictors of prosocial behavior, high levels of perceived social support were most strongly associated with prosocial behavior, followed by high levels of perceived stress, positive affect and psychological flexibility. Sociodemographic and psychosocial predictors of prosocial behavior were similar across regions.publishersversionPeer reviewe

Varietal description of two genotypes of manzano chili pepper (Capsicum pubescens Ruiz & Pav.)

Objective: the objective of this research work was to obtain the varietal description of two varieties of chile manzano in Las Montañas in the center of Veracruz, México. Design/methodology/approach: the varietal characterization module was established under greenhouse conditions at the Centro de Bachillerato Tecnológico Agropecuario No. 99 in the municipality of Coscomatepec de Bravo. The recorded descriptors were in accordance with the International of Plant Genetic Resources Institute for Capsicum and the Graphic Handbook for Variety Description of manzano hot pepper. The plants were characterized from the seedling to the adult plant. The agronomic management of the crop was carried out in accordance with the manual for the production of manzano hot pepper in Las Montañas of the state of Veracruz. Results: all qualitative descriptors were constant for the two varieties MEXUVNE1-15-C2 and MEXUVCU1-16-C2 from seedling to fruiting; in contrast, there were dissimilarities in plant height, stem, leaf, flower, fruit and seed dimensions. Limitations of the study/implications: the pandemic caused by COVID-19 was the main limitation so that some descriptors were not recorded in a timely manner as indicated in the Graphic Handbook. Findings/conclusions: both varieties are very similar; however, the greatest distinction was in the quantitative type descriptors such as: plant height, fruit length, fruit diameter and number of seeds.Objective: The objective of this research study was to obtain the varietal description of two varieties of manzano chili pepper in Las Montañas region, in central Veracruz, Mexico. Design/methodology/approach: The varietal characterization module was established under greenhouse conditions. The markers recorded were in accordance with the International Plant Genetic Resources Institute for Capsicum and the Graphic Handbook for Variety Description of manzano chili pepper. The plants were characterized from seedling in greenhouse to adult plant. The agronomic management of the crop was carried out in accordance with the manual for the production of manzano chili pepper in Las Montañas, state of Veracruz. Results: All qualitative markers were constant for the two varieties, MEXUVNE1-15-C2 and MEXUVCU1-16-C2, from seedling to fruit setting. In contrast, there were dissimilarities in plant height, and stem, leaf, flower, fruit and seed dimensions. Study limitations/implications: The pandemic caused by COVID-19 was the main limitation, resulting in some markers not being recorded in a timely manner as indicated in the Graphic Handbook. Findings/conclusions: Both varieties are very similar; however, the greatest distinction was in the quantitative markers, such as: plant height, fruit length, fruit diameter and number of seeds

Frequency and characteristics of familial melanoma in Spain: The FAM-GEM-1 Study

Similar to that observed in other countries, familial melanoma accounts for 6.6% of melanoma diagnoses in Spain. Although no differences in the multivariate analysis were found, some better prognosis factors, such as Breslow index, seem more frequent in familial melanoma, which reflect a better early detection marker and/or a different biological behavior

Reporte de atención clínica integral

El Proyecto de Aplicación Profesional en Atención Clínica Interdisciplinaria tuvo como objetivo general ofrecer servicios de atención y orientación profesional a los consultantes que la solicitaron para encontrar respuesta a los diferentes motivos de consulta psicológica y nutricional. Se les brindó el acompañamiento psicológico y nutricional a niños, adolescentes, adultos y familias en los siguientes escenarios: el Centro de Atención Psicológica, Centros de Desarrollo Comunitario del DIF Zapopan como DIF Arenales Tapatíos, DIF Santa María del Pueblito, DIF Santa Ana Tepetitlán Centro Polanco, Bufete Jurídico Clínica Ignacio Ellacuría y Clínica Nutricia. El proyecto ofreció principalmente espacios de atención en modalidad presencial y algunos en línea, donde se atendieron problemáticas tales como: consecuencias tras vivir la Pandemia de Covid-19, diferentes tipos de violencia, TCAS, problemas relacionales, depresión, ideación suicida, situaciones postraumáticas, y complicaciones en el desarrollo de una vida saludable tanto en los aspectos nutricionales como en el acceso a la justicia. Asimismo, se describen los múltiples aprendizajes tanto personales como profesionales, a través de las experiencias e intervenciones a lo largo del Proyecto de Aplicación Profesional.ITESO, A.C

Co-occurrence of mutations in NF1 and other susceptibility genes in pheochromocytoma and paraganglioma

IntroductionThe percentage of patients diagnosed with pheochromocytoma and paraganglioma (altogether PPGL) carrying known germline mutations in one of the over fifteen susceptibility genes identified to date has dramatically increased during the last two decades, accounting for up to 35-40% of PPGL patients. Moreover, the application of NGS to the diagnosis of PPGL detects unexpected co-occurrences of pathogenic allelic variants in different susceptibility genes.MethodsHerein we uncover several cases with dual mutations in NF1 and other PPGL genes by targeted sequencing. We studied the molecular characteristics of the tumours with co-occurrent mutations, using omic tools to gain insight into the role of these events in tumour development.ResultsAmongst 23 patients carrying germline NF1 mutations, targeted sequencing revealed additional pathogenic germline variants in DLST (n=1) and MDH2 (n=2), and two somatic mutations in H3-3A and PRKAR1A. Three additional patients, with somatic mutations in NF1 were found carrying germline pathogenic mutations in SDHB or DLST, and a somatic truncating mutation in ATRX. Two of the cases with dual germline mutations showed multiple pheochromocytomas or extra-adrenal paragangliomas - an extremely rare clinical finding in NF1 patients. Transcriptional and methylation profiling and metabolite assessment showed an “intermediate signature” to suggest that both variants had a pathological role in tumour development.DiscussionIn conclusion, mutations affecting genes involved in different pathways (pseudohypoxic and receptor tyrosine kinase signalling) co-occurring in the same patient could provide a selective advantage for the development of PPGL, and explain the variable expressivity and incomplete penetrance observed in some patients

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Socio-demographic analysis of status and level of nutrition and physical activity in two schools in Granada (Spain).

Objectives: Currently, nutritional habits and the lifestyle of Spanish schoolchildren have undergone important changes. This study aimed to analyze the sociodemographic characteristics, eating habits and physical activity of schoolchildren of two different schools and determine possible differences in nutritional status and body composition, as well as nutrition and physical activity level of school children. Methods: Cross-sectional study of 114 schoolchildren between 8 and 13 years old, from Granada, Spain. An assessment of the nutritional status was performed by anthropometry and an analysis of body composition by bioelectrical impedance. Two questionnaires were used, one ad hoc about sociodemographic and nutritional data and the Krece-Plus test to assess nutritional status and physical activity. Results: There are no noteworthy significant differences in the nutritional habits of schoolchildren in both centers, most performed five daily meals. There are significant differences in the use of transport to go to school; schoolchildren of the Albayzín attend to school walking everyday, with a higher prevalence of low weight and ideal fat percentage lower than recommended. On the other hand, the schoolchildren in the center of the capital have low regular physical activity and nutritional status. There are sociodemographic differences between the two populations of students, both comply with the recommendations on the number of daily intakes. Conclusion: Students who reside in the center of Granada are more sedentary than their counterparts in the Albayzín and have a higher prevalence of overweight and obesity. Students of the Albayzín have better nutrition and physical activity level

Panorama sanitario del cultivo de la soja - Campaña 2004/2005

Como ha venido sucediendo en las últimas 4 campañas en la mayor parte del área sojera de Tucumán y zonas de influencia, el ambiente durante el ciclo 2004/05 volvió a estar nuevamente dominado por prolongados períodos de sequía y temperaturas elevadas.  Esta situación trajo aparejado no sólo momentos de estrés para los cultivos sino también un menor desarrollo de las enfermedades.  A diferencia de años anteriores, en la campaña 2004/05 el déficit hídrico fue generalizado en intensidad en las diferentes zonas de la provincia.  A pesar de ello se observó la presencia diferencial de las enfermedades, observándose menores niveles de enfermedades en las zonas de mayor aptitud para el cultivo.  En general, la sanidad de los cultivos durante el ciclo fue muy buena.  En el presente trabajo se presentan los resultados del monitoreo de enfermedades de soja, llevados a cabo por la Sección Fitopatología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC) durante la campaña 2004/05.  Se incluyen los datos de las detecciones de roya de la soja, efectuadas en el marco del Programa Nacional de Roya de la Soja, del cual la EEAOC es institución fundadora.Fil: Ploper, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Gonzalez, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Galvez, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Zamorano, María A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Soldini, Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentin