RHOA (ras homolog gene family, member A)

Review on RHOA, with data on DNA/RNA, on the protein encoded and where the gene is implicated

Physiotherapy for treatment of lumbopelvic pain during pregnancy: systematic review

Introducción. Más de dos tercios de las mujeres embarazadas experimentan dolor lumbar y casi un quinto dolor pélvico. Objetivo. Revisar la evidencia empírica de los tratamientos de fisioterapia para el dolor lumbopélvico en el embarazo y comprobar su eficacia. Estrategia de búsqueda y selección de estudios. La búsqueda se realizó en las siguientes bases de datos: Pubmed, PEDro, Cochrane y LILACS. Se seleccionaron 14 ensayos clínicos aleatorios de acuerdo a los criterios de inclusión. Síntesis y análisis de los resultados. El ejercicio terapéutico, la terapia manual, el vendaje neuromuscular, la relajación muscular progresiva y el TENS fueron las intervenciones escogidas por los autores. Todos los estudios reportaron una reducción en la intensidad del dolor, pero no todos fueron estadísticamente significativos. El tratamiento con ejercicio es el más utilizado por los autores. Conclusiones. El ejercicio físico es eficaz para aliviar el dolor lumbopélvico en el embarazo y mejora la calidad de vida. Otras técnicas como el vendaje, la electroterapia y la terapia manual, también efectivas, pero menos estudiadas, podrían ser utilizadas como complementarias

Downregulation of microRNA-199b predicts unfavorable prognosis and emerges as a novel therapeutic target which contributes to PP2A inhibition in metastatic colorectal cancer

The tumor suppressor microRNA-199b (miR-199b) is a negative SET regulator associated with poor outcome in some human cancers. However, its expression levels as well as potential biological and clinical significance in colorectal cancer (CRC) remain completely unexplored. The PP2A inhibitor SET has shown promising therapeutic and clinical implications in metastatic CRC (mCRC) but the molecular mechanisms underlying SET deregulation are currently unknown. We show here miR-199b downregulation in 4 out of 5 CRC SET-overexpressing cell lines and its inverse correlation with SET overexpression in CRC patients. Moreover, miR-199b led to PP2A activation through a direct SET inhibition, impaired cell viability and enhanced oxaliplatin sensitivity in CRC cells. MiR-199b was found downregulated in 25% of cases, and associated with lymph metastasis (p = 0.049), presence of synchronous metastasis at diagnosis (p = 0.026) and SET overexpression (p < 0.001). Furthermore, low miR-199b levels determined shorter overall (p < 0.001), progression-free survival (p = 0.003) and predicted clinical benefit to oxaliplatin treatment. The miR-199b prognostic impact was particularly evident in both younger and KRAS wild-type subgroups. Multivariate analyses confirmed its independent prognostic impact. Altogether, our results show that miR-199b is a tumor suppressor whose downregulation independently determines worse outcome and emerges as a potential contributing mechanism to inhibit PP2A via SET overexpression in a subgroup of mCRC patient

Overlap at the molecular and immunohistochemical levels between angioimmunoblastic T-cell lymphoma and a subgroup of peripheral T-cell lymphomas without specific morphological features

The overlap of morphology and immunophenotype between angioimmunoblastic T-cell lymphoma (AITL) and other nodal peripheral T-cell lymphomas (n-PTCLs) is a matter of current interest whose clinical relevance and pathogenic background have not been fully established. We studied a series of 98 n-PTCL samples (comprising 57 AITL and 41 PTCL-NOS) with five TFH antibodies (CD10, BCL-6, PD-1, CXCL13, ICOS), looked for mutations in five of the genes most frequently mutated in AITL (TET2, DNMT3A, IDH2, RHOA and PLCG1) using the Next-Generation-Sequencing Ion Torrent platform, and measured the correlations of these characteristics with morphology and clinical features. The percentage of mutations in the RHOA and TET2 genes was similar (23.5% of cases). PLCG1 was mutated in 14.3%, IDH2 in 11.2% and DNMT3A in 7.1% of cases, respectively. In the complete series, mutations in RHOA gene were associated with the presence of mutations in IDH2, TET2 and DNMT3A (p < 0.001, p = 0.043, and p = 0.029, respectively). Fourteen cases featured RHOA mutations without TET2 mutations. A close relationship was found between the presence of these mutations and a TFH-phenotype in AITL and PTCL-NOS patients. Interestingly, BCL-6 expression was the only TFH marker differentially expressed between AITL and PTCL-NOS cases. There were many fewer mutated cases than there were cases with a TFH phenotype. Overall, these data suggest alternative ways by which neoplastic T-cells overexpress these proteins. On the other hand, no clinical or survival differences were found between any of the recognized subgroups of patients with respect to their immunohistochemistry or mutational profile.This work was supported by grants from the Instituto de Salud Carlos III, from the Ministerio de Economía, Industria y Competitividad (RTICC RD06/0020/0107, RD12/0036/0060, PI 12/1682, PT13/0010/0007, PI16/ 01294, SAF2013-47416-R, CIBERONC-ISCIII, PIE15/ 0081, ISCIII-MINECO AES-FEDER (Plan Estatal I+D+I 2013–2016): PI14/00221, PIE14/0064, PIE15/0081 and PIE16/01294)) and the Asociación Española Contra el Cáncer, Spain. JG-R is a recipient of an iPFIS predoctoral fellowship (IFI14/00003) from ISCIII-MINECO-AESFEDER (Plan Estatal I+D+I 2013–2016). MSB was supported by a Miguel Servet contract (CP11/00018) from the ISCIII-MINECO-AES-FEDER (Plan Nacional I+D+I 2008–2011), and currently holds a Miguel Servet II contract (CPII16/00024), supported by ISCIII-MINECOAES- FEDER (Plan Estatal I+D+I 2013–2016) and the Fundación de Investigación Biomédica Puerta de Hierro.S

Testing a fully autonomous robotic salesman in real scenarios

Over the past decades, the number of robots deployed in museums, trade shows and exhibitions have grown steadily. This new application domain has become a key research topic in the robotics community. Therefore, new robots are designed to interact with people in these domains, using natural and intuitive channels. Visual perception and speech processing have to be considered for these robots, as they should be able to detect people in their environment, recognize their degree of accessibility and engage them in social conversations. They also need to safely navigate around dynamic, uncontrolled environments. They must be equipped with planning and learning components, that allow them to adapt to different scenarios. Finally, they must attract the attention of the people, be kind and safe to interact with. In this paper, we describe our experience with Gualzru, a salesman robot endowed with the cognitive architecture RoboCog. This architecture synchronizes all previous processes in a social robot, using a common inner representation as the core of the system. The robot has been tested in crowded, public daily life environments, where it interacted with people that had never seen it before nor had a clue about its functionality. Experimental results presented in this paper demonstrate the capabilities of the robot and its limitations in these real scenarios, and define future improvement actions.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The cognitive architecture of a robotic salesman

This paper describes a robotics cognitive architecture for social robots named CORTEX. This architecture integrates di fferent levels of abstraction (from basic geometry to high-level predicates) into a unique Deep Space Representation (DSR) that diff erent agents interface. These agents update the contents of the DSR with new data from the outer world, and execute, plan and design behaviours. The design of CORTEX as an unified deep representation allows to fit both the subsymbolic processing and exibility requirements of robot control. In this paper a first implementation of CORTEX has been integrated into Gualzru, a robotic salesman, and tested in real scenarios. Results show that this cognitive architecture allows this robot to adequately execute its use case, and that it has a promising adaptability to achieve new tasks and be used in new scenarios.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Percepts symbols or Action symbols? Generalizing how all modules interact within a software architecture for cognitive robotics

Robots require a close coupling of perception and action. Cognitive robots go beyond this to require a further coupling with cognition. From the perspective of robotics, this coupling generally emphasizes a tightly integrated perceptuomotor system, which is then loosely connected to some limited form of cognitive system such as a planner. At the other end, from the perspective of automated planning, the emphasis is on a highly functional system that, taken to its extreme, calls perceptual and motor modules as independent functions. This paper proposes to join both perspectives through a unique representation where the responses of all modules on the software architecture (percepts or actions) are grounded using the same set of symbols. This allows to generalize the signal-to-symbol divide that separates classic perceptuomotor and automated planning systems, being the result a software architecture where all software modules interact using the same tokens.This paper has been partially supported by the Spanish Ministerio de Economía y Competitividad TIN2015-65686-C5 and FEDER funds and by the FP7 EU project ECHORD++ grant 601116 (CLARK project)

Tumor P70S6K hyperactivation is inversely associated with tumor-infiltrating lymphocytes in triple-negative breast cancer

Purpose: Triple-negative breast cancer (TNBC) is characterized by large heterogeneity and relative lack of available targeted therapies. To find therapeutic strategies for distinct patients with TNBC, several approaches have been used for TNBC clustering, including recently immune and phosphoproteomic patterns. Based on 70-kDa ribosomal protein S6 kinase (P70S6K)-TNBC clustering, the current study explores the immune profiling in TNBC tumors. Methods: Stromal tumor-infiltrating lymphocytes (sTILs) were evaluated in human TNBC tumor samples. Furthermore, immunohistochemistry staining for CD8, CD4, Foxp3, and CD20 was performed in tissue microarrays (TMA) sections. Results: Histological analysis showed decreased sTILs, CD20+ cells, and CD8+/CD4+ ratio in high phosphorylated P70S6K (p-P70S6K) tumors. Moreover, p-P70S6K score was directly correlated with CD4+ and Foxp3+ T cells, while it was inversely correlated with CD8+/CD4+ and CD8+/Foxp3+ ratios. Conclusion: sTIL infiltration and lymphocyte profiling vary in the context of hyperactivation of P70S6K in TNBC tumorsThe project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 893597. RC is a recipient of the ISCIII grants: PI17/01865 and PI20/01458. MQF is a recipient of the following Grants: AES-PI19/00454 funded by the ISCIII and co-funded by the European Regional Development Fund (ERDF) and B2017/BMD3733 (Immunothercan-CM)—Call for Coordinated Research Groups from Madrid Region—Madrid Regional Government—ERDF funds. The study was also funded by CRIS Contra el Cancer Foundatio

Perceptions or Actions? Grounding How Agents Interact Within a Software Architecture for Cognitive Robotics

One of the aims of cognitive robotics is to endow robots with the ability to plan solutions for complex goals and then to enact those plans. Additionally, robots should react properly upon encountering unexpected changes in their environment that are not part of their planned course of actions. This requires a close coupling between deliberative and reactive control flows. From the perspective of robotics, this coupling generally entails a tightly integrated perceptuomotor system, which is then loosely connected to some specific form of deliberative system such as a planner. From the high-level perspective of automated planning, the emphasis is on a highly functional system that, taken to its extreme, calls perceptual and motor modules as services when required. This paper proposes to join the perceptual and acting perspectives via a unique representation where the responses of all software modules in the architecture are generalized using the same set of tokens. The proposed representation integrates symbolic and metric information. The proposed approach has been successfully tested in CLARC, a robot that performs Comprehensive Geriatric Assessments of elderly patients. The robot was favourably appraised in a survey conducted to assess its behaviour. For instance, using a 5-point Likert scale from 1 (strongly disagree) to 5 (strongly agree), patients reported an average of 4.86 when asked if they felt confident during the interaction with the robot. This paper proposes a mechanism for bringing the perceptual and acting perspectives closer within a distributed robotics architecture. The idea is built on top of the blackboard model and scene graphs. The modules in our proposal communicate using a short-term memory, writing the perceptual information they need to share with other agents and accessing the information they need for determining the next goals to address