Lysine and Arginine Reduce the Effects of Cerebral Ischemic Insults and Inhibit Glutamate-Induced Neuronal Activity in Rats

Intravenous administration of arginine was shown to be protective against cerebral ischemic insults via nitric oxide production and possibly via additional mechanisms. The present study aimed at evaluating the neuroprotective effects of oral administration of lysine (a basic amino acid), arginine, and their combination on ischemic insults (cerebral edema and infarction) and hemispheric brain swelling induced by transient middle cerebral artery occlusion/reperfusion in rats. Magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining were performed 2 days after ischemia induction. In control animals, the major edematous areas were observed in the cerebral cortex and striatum. The volumes associated with cortical edema were significantly reduced by lysine (2.0 g/kg), arginine (0.6 g/kg), or their combined administration (0.6 g/kg each). Protective effects of these amino acids on infarction were comparable to the inhibitory effects on edema formation. Interestingly, these amino acids, even at low dose (0.6 g/kg), were effective to reduce hemispheric brain swelling. Additionally, the effects of in vivo microiontophoretic (juxtaneuronal) applications of these amino acids on glutamate-evoked neuronal activity in the ventromedial hypothalamus were investigated in awake rats. Glutamate-induced neuronal activity was robustly inhibited by microiontophoretic applications of lysine or arginine onto neuronal membranes. Taken together, our results demonstrate the neuroprotective effects of oral ingestion of lysine and arginine against ischemic insults (cerebral edema and infarction), especially in the cerebral cortex, and suggest that suppression of glutamate-induced neuronal activity might be the primary mechanism associated with these neuroprotective effects

A Study of community nursing Practice of basic nursing educaition curriculum - Analysis of practice repord by student nurses -

地域看護実習は、地域における看護の役割を理解することを主なねらいとして、保健所で実習を行っているが、保健所実習が地域看護実習の目標を達成しているかは否かを、学生の実習記録を分析し検討した。80％以上の学生が体験している実習内容は、家庭訪問、集団検診、共同作業所、健康相談等である。実習目標の記載状況は「実習項目別実習記録」及び実習全体のまとめ「総括」の記載内容から抽出し分析した。目標記載率70％以上であった実習項目は、実習目標①（地域の人々の生活及び健康問題の理解）は共同作業所、グループ指導、家庭訪問であり、目標②（保健サービスの実態の理解）では家庭訪問であった。また「総括」では目標②の記載率が高く、目標③（地域の看護活動の実態を学ぶ）の記載率67％と予測に反して高くなかった。今後地域看護、継続看護に関する講義のあり方を含めて、地域看護実習の位置づけを再検討する必要がある。Practice reports at the health center for 1 week written by 152 student nurses were analysed to know wether they achieved their goal of the course for community nursing or not. More than 80% students attended at home visits, mass health examination, common workshops for mentally disordered persons and health counsclling. Practice for understanding peoples'lives and health problems in the community were well achieved in practice at the common workshop, group guidance and home visit. They understood about health care and servicesin the community through home visits. Their comprehension of role of the community nursing was not so sufficient as expected for mark of curriculum

ESTIMATION OF SOIL CARBON STORAGE IN SHINJUKU GYOEN NATIONAL GARDEN BASED ON LAND USE HISTORY AND SOIL COMPACTNESS PROFILES

This study aims to estimate soil carbon storage in Shinjuku Gyoen National Garden,Tokyo Metropolis. Undisturbed soil samples were collected in the Gyoen from regions of differentland use using a core cylinder. A modified investigation was used by combining measurement ofvertical structure of soil compactness in order to estimate cumulative carbon content withoutinterfering with the park landscape. Softness obtained using a cone penetrometer was converted tosoil bulk density (g cm-3) and samples for measurement of total carbon content (g kg-1) werecollected using a boring stick. The carbon storage in the Gyoen (0 – 100 cm) was estimated as 316± 83.1 t C ha-1 in turf areas and 198 ± 61.3 t C ha-1 in forest areas, respectively, which areequivalent or slightly larger than the standard values obtained for Andosols and Brown Forest soils,respectively. The large difference in the cumulative carbon storage between turf area and forestarea for the entire profile (0 – 100 cm) can be explained by the difference in the pedogenesisprocess of humus accumulation together with the diagenesis process of humus decomposition,both of which depend on the humus quality of vegetation and land use history. The use of theSoftness obtained from measurement of vertical soil compactness was discussed as a proxy ofbulk density. It is shown that vertical soil compactness differs in same land use regions in theGyoen. Although the estimated values of cumulative carbon content had issues withoverestimation in some cases, we suggest that the use of the Softness as a substitute for bulkdensity is applicable for estimating soil carbon storage. Subsurface structure, regulated by land usehistory and land creating history should be taken into account for the reliable evaluation of soilcarbon storage in urban areas

Multi-modal acoustic-photo-acoustic imaging for small animal imaging

Peer reviewed: YesNRC publication: Ye

Flexible Ultrasonic Transducers and Their Performance

Peer reviewed: YesNRC publication: Ye

Lifestyle factors and visible skin aging in a population of Japanese elders.

BACKGROUND: The number of studies that use objective and quantitative methods to evaluate facial skin aging in elderly people is extremely limited, especially in Japan. Therefore, in this cross-sectional study we attempted to characterize the condition of facial skin (hyperpigmentation, pores, texture, and wrinkling) in Japanese adults aged 65 years or older by using objective and quantitative imaging methods. In addition, we aimed to identify lifestyle factors significantly associated with these visible signs of aging. METHODS: The study subjects were 802 community-dwelling Japanese men and women aged at least 65 years and living in the town of Kurabuchi (Takasaki City, Gunma Prefecture, Japan), a mountain community with a population of approximately 4800. The facial skin condition of subjects was assessed quantitatively using a standardized facial imaging system and subsequent computer image analysis. Lifestyle information was collected using a structured questionnaire. The association between skin condition and lifestyle factors was examined using multivariable regression analysis. RESULTS: Among women, the mean values for facial texture, hyperpigmentation, and pores were generally lower than those among age-matched men. There was no significant difference between sexes in the severity of facial wrinkling. Older age was associated with worse skin condition among women only. After adjusting for age, smoking status and topical sun protection were significantly associated with skin condition among both men and women. CONCLUSIONS: Our study revealed significant differences between sexes in the severity of hyperpigmentation, texture, and pores, but not wrinkling. Smoking status and topical sun protection were significantly associated with signs of visible skin aging in this study population

Statistics of 207 Lya Emitters at a Redshift Near 7: Constraints on Reionization and Galaxy Formation Models

We present Lya luminosity function (LF), clustering measurements, and Lya line profiles based on the largest sample, to date, of 207 Lya emitters (LAEs) at z=6.6 on the 1-deg^2 sky of Subaru/XMM-Newton Deep Survey (SXDS) field. Our z=6.6 Lya LF including cosmic variance estimates yields the best-fit Schechter parameters of phi*=8.5 +3.0/-2.2 x10^(-4) Mpc^(-3) and L*(Lya)=4.4 +/-0.6 x10^42 erg s^(-1) with a fixed alpha=-1.5, and indicates a decrease from z=5.7 at the >~90% confidence level. However, this decrease is not large, only =~30% in Lya luminosity, which is too small to be identified in the previous studies. A clustering signal of z=6.6 LAEs is detected for the first time. We obtain the correlation length of r_0=2-5 h^(-1) Mpc and bias of b=3-6, and find no significant boost of clustering amplitude by reionization at z=6.6. The average hosting dark halo mass inferred from clustering is 10^10-10^11 Mo, and duty cycle of LAE population is roughly ~1% albeit with large uncertainties. The average of our high-quality Keck/DEIMOS spectra shows an FWHM velocity width of 251 +/-16 km s^(-1). We find no large evolution of Lya line profile from z=5.7 to 6.6, and no anti-correlation between Lya luminosity and line width at z=6.6. The combination of various reionization models and our observational results about the LF, clustering, and line profile indicates that there would exist a small decrease of IGM's Lya transmission owing to reionization, but that the hydrogen IGM is not highly neutral at z=6.6. Our neutral-hydrogen fraction constraint implies that the major reionization process took place at z>~7.Comment: 28 pages, 23 figures. Accepted for publication in Ap