Genetic factors regulating lung vasculature and immune cell functions associate with resistance to pneumococcal infection

Streptococcus pneumoniae is an important human pathogen responsible for high mortality and morbidity worldwide. The susceptibility to pneumococcal infections is controlled by as yet unknown genetic factors. To elucidate these factors could help to develop new medical treatments and tools to identify those most at risk. In recent years genome wide association studies (GWAS) in mice and humans have proved successful in identification of causal genes involved in many complex diseases for example diabetes, systemic lupus or cholesterol metabolism. In this study a GWAS approach was used to map genetic loci associated with susceptibility to pneumococcal infection in 26 inbred mouse strains. As a result four candidate QTLs were identified on chromosomes 7, 13, 18 and 19. Interestingly, the QTL on chromosome 7 was located within S. pneumoniae resistance QTL (Spir1) identified previously in a linkage study of BALB/cOlaHsd and CBA/CaOlaHsd F2 intercrosses. We showed that only a limited number of genes encoded within the QTLs carried phenotype-associated polymorphisms (22 genes out of several hundred located within the QTLs). These candidate genes are known to regulate TGFb signalling, smooth muscle and immune cells functions. Interestingly, our pulmonary histopathology and gene expression data demonstrated, lung vasculature plays an important role in resistance to pneumococcal infection. Therefore we concluded that the cumulative effect of these candidate genes on vasculature and immune cells functions as contributory factors in the observed differences in susceptibility to pneumococcal infection. We also propose that TGFbmediated regulation of fibroblast differentiation plays an important role in development of invasive pneumococcal disease.This work was supported by the European Union-funded Pneumopath Project HEALTH-F3-2009-222983. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer-reviewedPublisher Versio

Primary Care Antibiotic Prescribing and Infection-Related Hospitalisation

Inappropriate prescribing of antibiotics has been widely recognised as a leading cause of antimicrobial resistance, which in turn has become one of the most significant threats to global health. Given that most antibiotic prescriptions are issued in primary care settings, investigating the associations between primary care prescribing of antibiotics and subsequent infection-related hospitalisations affords a valuable opportunity to understand the long-term health implications of primary care antibiotic intervention. A narrative review of the scientific literature studying associations between primary care antibiotic prescribing and subsequent infection-related hospitalisation was conducted. The Web of Science database was used to retrieve 252 potentially relevant studies, with 23 of these studies included in this review (stratified by patient age and infection type). The majority of studies (n = 18) were published in the United Kingdom, while the remainder were conducted in Germany, Spain, Denmark, New Zealand, and the United States. While some of the reviewed studies demonstrated that appropriate and timely antibiotic prescribing in primary care could help reduce the need for hospitalisation, excessive antibiotic prescribing can lead to antimicrobial resistance, subsequently increasing the risk of infection-related hospitalisation. Few studies reported no association between primary care antibiotic prescriptions and subsequent infection-related hospitalisation. Overall, the disparate results in the extant literature attest to the conflicting factors influencing the decision-making regarding antibiotic prescribing and highlight the necessity of adopting a more patient-focussed perspective in stewardship programmes and the need for increased use of rapid diagnostic testing in primary care

Parenting and child mental health during the COVID-19 pandemic:An online study with Portuguese and British Families

COVID-19 and the subsequent public health response created many additional stresses for families. We examined parental behaviour during the COVID-19 pandemic in two European Countries and explored the association between parents’ behaviour and children’s anxiety and quality of life. Caregivers of children and adolescents (N = 442; 86.7% mothers) between 6 and 16 years old (M = 10, SD = 2.85) participated in an online cross-sectional survey in Portugal and the United Kingdom. Results show that higher children’s anxiety and lower quality of life were associated with higher levels of unrealistic parental demands, lower parental self-care, and higher parental emotional dysregulation. Encouragement of children’s emotion expression and management of exposure to COVID-19 information was negatively associated with children’s anxiety. Promotion of routines, support of children’s emotion modulation and promotion of children’s healthy lifestyles were positively associated with children’s quality of life. The predictors differed according to country and age group. These results highlight the importance of specific parenting behaviours on children’s mental health during COVID-19. The need to moderate unrealistic demands and attend to parental self-care to reduce parental emotional dysregulation is important.Parentalidad y salud mental infantil durante la pandemia de COVID-19: Un estudio online con familias Portuguesas y Británicas. COVID-19 y la subsiguiente respuesta de salud pública crearon muchas tensiones adicionales para las familias. Examinamos el comportamiento de los padres durante la pandemia de COVID-19 en dos países europeos y exploramos la asociación entre el comportamiento de los padres y la ansiedad y la calidad de vida del niño. Cuidadores de niños y adolescentes (N = 442; 86.7% madres) entre 6 y 16 años (M = 10, DT = 2,85) participaran en una online encuesta en Portugal y en el Reino Unido. En los resultados se observa que la mayor ansiedad y una menor calidad de vida de los niños se asociaron con niveles más altos de demandas parentales poco realistas, menor autocuidado y mayor desregulación emocional de los padres. El estímulo a la expresión de las emociones de los niños y el manejo de la exposición a la información de COVID-19 se asoció negativamente con la ansiedad de los niños. La promoción de rutinas, el apoyo a la modulación de las emociones de los niños y la promoción de estilos de vida saludables de los niños se asociaron positivamente con la calidad de vida de los niños. Los predictores difirieron según el país y el grupo de edad. Estos resultados resaltan la importancia de comportamientos parentales específicos en la salud mental de los niños durante el COVID-19. La necesidad de moderar las demandas poco realistas y prestar atención al autocuidado de los padres para reducir la desregulación emocional de los padres es important

Parenting and child mental health during the COVID-19 pandemic:An online study with Portuguese and British Families

COVID-19 and the subsequent public health response created many additional stresses for families. We examined parental behaviour during the COV-ID-19 pandemic in two European Countries and explored the association between parents’ behaviour and children’s anxiety and quality of life. Caregivers of children and adolescents (N = 442; 86.7% mothers) between 6 and 16 years old (M = 10, SD = 2.85) participated in an online cross-sectional survey in Portugal and the United Kingdom. Results show that higher children’s anxiety and lower quality of life were associated with higher levels of unrealistic parental demands, lower parental self-care, and higher parental emotional dysregulation. Encouragement of children’s emotion expression and management of exposure to COVID-19 information was negatively associated with children’s anxiety. Promotion of routines, support of children’s emotion modulation and promotion of children’s healthy lifestyles were positively associated with children’s quality of life. The predictors differed according to country and age group. These results highlight the importance of specific parenting behaviours on children’s mental health during COVID-19. The need to moderate unrealistic demands and attend to parental self-care to reduce parental emotional dysregulation is important.</p

Characterization and Quantification of Isoprene-Derived Epoxydiols in Ambient Aerosol in the Southeastern United States

Isoprene-derived epoxydiols (IEPOX) are identified in ambient aerosol samples for the first time, together with other previously identified isoprene tracers (i.e., 2-methyltetrols, 2-methylglyceric acid, C5-alkenetriols, and organosulfate derivatives of 2-methyltetrols). Fine ambient aerosol collected in downtown Atlanta, GA and rural Yorkville, GA during the 2008 August Mini-Intensive Gas and Aerosol Study (AMIGAS) was analyzed using both gas chromatography/quadrupole mass spectrometry (GC/MS) and gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) with prior trimethylsilylation. Mass concentrations of IEPOX ranged from ~1 to 24 ng m^(−3) in the aerosol collected from the two sites. Detection of particle-phase IEPOX in the AMIGAS samples supports recent laboratory results that gas-phase IEPOX produced from the photooxidation of isoprene under low-NO_x conditions is a key precursor of ambient isoprene secondary organic aerosol (SOA) formation. On average, the sum of the mass concentrations of IEPOX and the measured isoprene SOA tracers accounted for about 3% of the organic carbon, demonstrating the significance of isoprene oxidation to the formation of ambient aerosol in this region

From So Far to So Close. Addressing the Refugee Phenomenon: History, Sociology, Technology

European project Enhancing Social Cohesion through Sharing the Cultural Heritage of Forced Migrations - SO-CLOSELa Conferència internacional "From So Far to So Close. Addressing the Refugee Phenomenon: History, Sociology, Technology" es va celebrar en format virtual els dies 15 i 16 de juny de 2020. La primera taula rodona "History of Refugees in the 20th Century" va incloure les següents intervencions: 1. Violence, Civil War and Female Forced Displacement : the case of Trikeri (Greece) / Magda Fitili -- 2. After the war. Communist mass violence and forced displacements in Eastern Europe after 1945 / Virgiliu Ţârău -- 3. Mass Violence and Displacement during the 1990s from former Yugoslavia: On Overview with a focus onBosnia and Herzegovina / Dženeta Karabegović -

Intraparenchymal Striatal Transplants Required for Maintenance of Behavioral Recovery in an Animal Model of Huntington's Disease

Rats which receive injections of kainic acid (KA) into the striatum show many of the anatomical, biochemical and behavioral abnormalities seen in patients with Huntington's disease. Recently, it has been reported that fetal striatal transplants into the lesioned striatum could normalize the neurological and behavioral abnormalities produced by the KA lesion. The present study examined the issue of transplant integration in producing behavioral recovery. In one experiment, lesioned animals with transplants located within the lateral ventricle were compared against parenchymally transplanted rats. It was found that unless the ventricular transplant grew into the lesioned striatum there was no recovery. The second experiment demonstrated that electrolytic destruction of a successful fetal striatal transplant could reverse the transplant-induced behavioral recovery. These results suggest that the integrity of the transplant is important in maintaining behavioral recovery. A continuing functional interaction between the host brain and transplanted tissue may be a vital element in the success of the fetal striatal transplant

AgentSeal : agent-based model describing movement of marine central-place foragers

Acknowledgement This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 746602. GA and SB have been partly funded by Gemini Wind park and the NWO (project ALWPP.2017.003). We would like to thank J. Grecian, D. Thomson, M. Fedak, M. Carter, D. Russell, A. Hall, J. Ransijn, H. Vance and M. Civil for help in model design.Peer reviewedPublisher PD

Effect of pediatric physical therapy on deformational plagiocephaly in children with positional preference: a randomized controlled trial

Objective To study the effect of pediatric physical therapy on positional preference and deformational plagiocephaly.\ud \ud Design Randomized controlled trial.\ud \ud Setting Bernhoven Hospital, Veghel, the Netherlands.\ud \ud Participants Of 380 infants referred to the examiners at age 7 weeks, 68 (17.9%) met criteria for positional preference, and 65 (17.1%) were enrolled and followed up at ages 6 and 12 months.\ud \ud Intervention Infants with positional preference were randomly assigned to receive either physical therapy (n = 33) or usual care (n = 32).\ud \ud Main Outcome Measures The primary outcome was severe deformational plagiocephaly assessed by plagiocephalometry. The secondary outcomes were positional preference, motor development, and cervical passive range of motion.\ud \ud Results Both groups were comparable at baseline. In the intervention group, the risk for severe deformational plagiocephaly was reduced by 46% at age 6 months (relative risk, 0.54; 95% confidence interval, 0.30-0.98) and 57% at age 12 months (0.43; 0.22-0.85). The numbers of infants with positional preference needed to treat were 3.85 and 3.13 at ages 6 and 12 months, respectively. No infant demonstrated positional preference at follow-up. Motor development was not significantly different between the intervention and usual care groups. Cervical passive range of motion was within the normal range at baseline and at follow-up. When infants were aged 6 months, parents in the intervention group demonstrated significantly more symmetry and less left orientation in nursing, positioning, and handling.\ud \ud Conclusion A 4-month standardized pediatric physical therapy program to treat positional preference significantly reduced the prevalence of severe deformational plagiocephaly compared with usual care

Cumulative Risk Exposure and Social Isolation as Correlates of Carer and Child Mental Health During the COVID-19 Pandemic:An Online Study with Families from Various Europeans Countries

This study adopted a cumulative risk approach to examine the relations between various domains of risk factors (i.e., social isolation and home confinement, other pandemic-related risk factors, and pre-existing psychosocial risk factors) and carers’ and children’s mental health during the first wave of the COVID-19 pandemic in Europe. The sample consisted of 1475 carers of 6- to 16-year-old children and adolescents residing in five European countries (Portugal, United Kingdom, Romania, Spain, and The Netherlands) who completed an online survey. The results showed that each of the three domains of adversity accounted for unique variation in carers’ and children’s mental health outcomes. Also, the results indicated that pre-existing psychosocial risk factors moderated the relationship between pandemic-related risk factors and children and carers’ anxiety and between social isolation and confinement and carers’ well-being. Simple slopes analysis suggested a stronger relationship between these domains of adversities and mental health outcomes in already more vulnerable families. It is important to consider the implications of social isolation measures and confinement for families’ mental health, paying special attention to families with pre-existing psychosocial vulnerabilities. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10578-021-01233-3