9 research outputs found
Modulation of macrophages differentiation by nanoscale-engineered geometric and chemical features
Macrophage differentiation into M1 (inflammatory) and M2 (healing) phenotypes plays a vital role in determining the fate of biomaterials. The biophysical properties of the extracellular matrix are known to affect macrophage behavior. Mimicking these special biophysical properties of the extracellular matrix has led to increasing interest in biomaterial constructs with tailor-engineered surface nanotopographical and chemical properties. However, a significant gap of knowledge exists in the role played by the combinational effect of surface nanotopography and chemistry. To address this gap, we have fabricated nanoporous surfaces of controlled pore size (30, 65, and 200 nm) and lateral spacing with uniform outermost surface chemistry tailored with amines (NH2), carboxyl (COOHâ) and hydrocarbon (CH3â) functionalities. We show that the combinatory effects of surface properties can direct the differentiation of macrophages to the pro-healing M2 phenotype. This is most evident on the surface featuring nanopores of 200 nm and âCOOH functionality. Overall, the concentration of pro-inflammatory cytokines significantly decreases, while the concentration of anti-inflammatory cytokines increases many folds on nanotopographically, and chemically, modified surfaces compared to their planar counterparts. Our data provide pioneering knowledge that could provide pathways to tuning inflammatory and foreign body responses and instruct the design of tailor-engineered biomaterial implants to enable better clinical outcomes.A. Bachhuka, R. Madathiparambil Visalakshan, C. S. Law, A. Santos, H. Ebendorff-Heidepriem, S. Karnati, and K. Vasile
Correction to "Tuning chemistry and topography of nanoengineered surfaces to manipulate immune response for bone regeneration applications"
After online publication of this Article, the authors noticed an error in the Supplementary Figure 3 lower magnification of 38AApp SEM image. The correct Supplementary Figure 3 of this Article should have appeared as below. The authors apologize for any inconvenience caused. (Figure Presented).</p
Protein Interactions with Nanoengineered Polyoxazoline Surfaces Generated via Plasma Deposition
Protein adsorption
to biomaterials is critical in determining their
suitability for specific applications, such as implants or biosensors.
Here, we show that surface nanoroughness can be tailored to control
the covalent binding of proteins to plasma-deposited polyoxazoline
(PPOx). Nanoengineered surfaces were created by immobilizing gold
nanoparticles varying in size and surface density on PPOx films. To
keep the surface chemistry consistent while preserving the nanotopography,
all substrates were overcoated with a nanothin PPOx film. Bovine serum
albumin was chosen to study protein interactions with the nanoengineered
surfaces. The results demonstrate that the amount of protein bound
to the surface is not directly correlated with the increase in surface
area. Instead, it is determined by nanotopography-induced geometric
effects and surface wettability. A densely packed array of 16 and
38 nm nanoparticles hinders protein adsorption compared to smooth
PPOx substrates, while it increases for 68 nm nanoparticles. These
adaptable surfaces could be used for designing biomaterials where
proteins adsorption is or is not desirable
The introduction of nanotopography suppresses bacterial adhesion and enhances osteoinductive capacity of plasma deposited polyoxazoline surface
The plasma deposited polyoxazoline (PPOx) has been emerging in biomedical applications, especially for the surface modification of bone tissue engineering scaffold and/or bone implants. Herein, PPOx surfaces were generated by plasma polymerization with the introduction of surface nanotopography gradient, achieved by immobilization of different density of 16 nm gold nanoparticles. The introduction of surface nanotopography suppressed the adhesion of S. aureus on PPOx surface. Moreover, the introduction of surface nanotopography enhanced the initial attachment and spreading of hMSCs, as well as promoted the osteogenic differentiation of hMSCs. RhoA/ROCK signaling pathway may be involved in the enhancement of osteoinductive capacity of PPOx surface by nanotopography
Synergistic Effect of Surface Chemistry and Surface Topography Gradient on Osteogenic/Adipogenic Differentiation of hMSCs
Much attention has been paid to understanding the individual effects of surface chemistry or topography on cell behavior. However, the synergistic influence of both surface chemistry and surface topography on differentiation of human mesenchymal stem cells (hMSCs) should also be addressed. Here, gold nanoparticles were immobilized in an increasing number density manner to achieve a surface topography gradient; a thin film rich in amine (-NH2) or methyl (-CH3) chemical groups was plasma-polymerized to adjust the surface chemistry of the outermost layer (ppAA and ppOD, respectively). hMSCs were cultured on these model substrates with defined surface chemistry and surface topography gradient. The morphology and focal adhesion (FA) formation of hMSCs were first examined. hMSC differentiation was then co-induced in osteogenic and adipogenic medium, as well as in the presence of extracellular-signal-regulated kinase1/2 (ERK1/2) and RhoA/Rho-associated protein kinase (ROCK) inhibitors. The results show that the introduction of nanotopography could enhance FA formation and osteogenesis but inhibited adipogenesis on both ppAA and ppOD surfaces, indicating that the surface chemistry could regulate hMSC differentiation, in a surface topography-dependent manner. RhoA/ROCK and ERK1/2 signaling pathways may participate in this process. This study demonstrated that surface chemistry and surface topography can jointly affect cell morphology, FA formation, and thus osteogenic/adipogenic differentiation of hMSCs. These findings highlight the importance of the synergistic effect of different material properties on regulation of cell response, which has important implications in designing functional biomaterials.Xujie Liu, Yakun Wang, Yan He, Xiaofeng Wang, Ranran Zhang, Akash Bachhuka, Rahul Madathiparambil Visalakshan, Qingling Feng, and Krasimir Vasile
Ultra-small gold nanoclusters assembled on plasma polymer-modified zeolites : A multifunctional nanohybrid with anti-haemorrhagic and anti-inflammatory properties
Hemostatic agents are pivotal for managing clinical and traumatic bleeding during emergency and domestic circumstances. Herein, a novel functional hybrid nanocomposite material consisting of plasma polymer-modified zeolite 13X and ultra-small gold nanoclusters (AuNCs) was fabricated as an efficient hemostatic agent. The surface of zeolite 13X was functionalised with amine groups which served as binding sites for carboxylate terminated AuNCs. Protein corona studies revealed the enhanced adsorption of two proteins, namely, coagulation factors and plasminogen as a result of AuNCs immobilization on the zeolite surface. The immune response studies showed that the hybrid nanocomposites are effective in reducing inflammation, which combined with a greater attachment of vitronectin, may promote wound healing. The hemostatic potential of the nanocomposite could be directly correlated with their immunomodulatory and anti-haemorrhagic properties. Together, the hybrid nanoengineered material developed in this work could provide a new avenue to tackle life-threatening injuries in civilian and other emergencies.</p
Plasma polymerized bio-interface directs fibronectin adsorption and functionalization to enhance âepithelial barrier structureâ formation via FN-ITG ÎČ1-FAK-mTOR signaling cascade
Background: Transepithelial medical devices are increasing utilized in clinical practices. However, the damage of continuous natural epithelial barrier has become a major risk factor for the failure of epithelium-penetrating implants. How to increase the âepithelial barrier structuresâ (focal adhesions, hemidesmosomes, etc.) becomes one key research aim in overcoming this difficulty. Directly targeting the in situ âepithelial barrier structuresâ related proteins (such as fibronectin) absorption and functionalization can be a promising way to enhance interface-epithelial integration. Methods: Herein, we fabricated three plasma polymerized bio-interfaces possessing controllable surface chemistry. Their capacity to adsorb and functionalize fibronectin (FN) from serum protein was compared by Liquid Chromatography-Tandem Mass Spectrometry. The underlying mechanisms were revealed by molecular dynamics simulation. The response of gingival epithelial cells regarding the formation of epithelial barrier structures was tested. Results: Plasma polymerized surfaces successfully directed distinguished protein adsorption profiles from serum protein pool, in which plasma polymerized allylamine (ppAA) surface favored adsorbing adhesion related proteins and could promote FN absorption and functionalization via electrostatic interactions and hydrogen bonds, thus subsequently activating the ITG ÎČ1-FAK-mTOR signaling and promoting gingival epithelial cells adhesion. Conclusion: This study offers an effective perspective to overcome the current dilemma of the inferior interface-epithelial integration by in situ protein absorption and functionalization, which may advance the development of functional transepithelial biointerfaces. Graphical Abstract: Tuning the surface chemistry by plasma polymerization can control the adsorption of fibronectin and functionalize it by exposing functional protein domains. The functionalized fibronectin can bind to human gingival epithelial cell membrane integrins to activate epithelial barrier structure related signaling pathway, which eventually enhances the formation of epithelial barrier structure.</p