Transcription of ribosomal genes can cause nondisjunction

Mitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These findings demonstrate that highly transcribed loci can cause chromosome nondisjunction

Interaction of Mediterranean water eddies with Sedlo and Seine Seamounts, Subtropical Northeast Atlantic

13 pages, 10 figures, 2 tablesDuring years 2003 and 2004 both Sedlo and Seine Seamounts, lying in the area of influence of the Mediterranean water outflow in the Northeast Atlantic, were investigated in the framework of the EU-funded project OASIS. Analysis of CTD data shows that the levels of major variability in temperature and salinity were confined to the upper ocean layer, which is subject to seasonal air–sea heat exchange, and to the Mediterranean Water (MW) level. In this paper we concentrate on the latter case and show that the variability observed resulted from Mediterranean water eddies (Meddies) colliding with the seamounts. Combined data from CTD surveys, current meter moorings, ship-mounted ADCP and satellite altimetry suggest that during the period of observations there was only one Meddy interacting with each of the seamounts. On Sedlo Seamount intensive interaction resulted in the loss by the Meddy of about 25% of the salt around the seamount, whereas on Seine the Meddy (or portion of it) escaped the seamount after performing several rotations around the summit without any significant interactionThis work was accomplished with funding from the European Union under project OASIS (Ref. EVK3-2001-00152-OASIS)Peer reviewe

The role of Ynt1 in nitrate and nitrite transport in the yeast Hansenula polymorpha

Ynt1 is the only high-affinity nitrate uptake system in Hansenula polymorpha. Nitrate uptake was directly correlated with the Ynt1 levels and shown to be independent of nitrate reductase (NR) activity levels. Ynt1 failed to transport chlorate and, as a result, strains lacking YNT1 were sensitive to chlorate, as is the wild-type. Nitrite uptake in a wild-type strain was partially inhibited by nitrate to levels shown by a YNT1-disrupted strain in which, in turn, nitrite transport was not inhibited by nitrate. It is concluded that nitrite uptake takes place by two different transport systems: Ynt1 and a nitrite-specific transporter(s). The nitrite-specific transport system was induced by nitrate; consistently, no induction was observed in strains lacking the transcription factor YNA1, which is involved in nitrate and nitrite induction of the nitrate assimilatory structural genes. Ynt1 presents its optimal rate for nitrite uptake at pH 6, while pH 4 was optimal for the specific nitrite uptake system(s). At pH 5.5, the contribution of Ynt1 to high-affinity nitrate and nitrite uptake was around 95% and 60%, respectively. The apparent Km of Ynt1 for nitrate and nitrite is in the µM range, as is the specific nitrite uptake system for nitrite. The analysis of the effect of the reduced nitrogen sources on nitrate assimilation revealed that glutamine inactivates nitrate and nitrite transport, dependent on Ynt1, but not the nitrite-specific system.