3 research outputs found
Quantum-Chemical Studies on the Favored and Rare Tautomers of Neutral and Redox Adenine
All possible twenty-three prototropic tautomers of neutral
and
redox adenine (nine amine and fourteen imine forms, including geometric
isomerism of the exo î»NH group) were examined in vacuo {DFTÂ(B3LYP)/6-311+GÂ(d,p)}.
The NH â NH conversions as well as those usually omitted, NH
â CH and CH â CH, were considered. An interesting change
of the tautomeric preference occurs when proceeding from neutral to
reduced adenine. One-electron reduction favors the nonaromatic amine
C8HâN10H tautomer. This tautomeric preference is similar to
that (C2H) for reduced imidazole. Water molecules (PCM model) seem
to not change this trend. They influence solely the relative energies.
The DFT vertical detachment energy in the gas phase is positive for
each tautomer, e.g., 0.03 eV for N9HâN10H and 1.84 eV for C8HâN10H.
The DFT adiabatic electron affinity for the favored process, neutral
N9HâN10H â reduced C8HâN10H (ground states),
is equal to 0.18 eV at 0 K (ZPE included). One-electron oxidation
does not change the tautomeric preference in the gas phase. The aromatic
amine N9HâN10H tautomer is favored for the oxidized molecule
similarly as for the neutral one. The DFT adiabatic ionization potential
for the favored process, neutral N9HâN10H â oxidized
N9HâN10H (ground states), is equal to 8.12 eV at 0 K (ZPE included).
Water molecules (PCM model) seem to influence solely the composition
of the tautomeric mixture and the relative energies. They change the
energies of the oxidation and reduction processes by ca. 2 eV
Cryogenian evolution of stigmasteroid biosynthesis
International audienceSedimentary hydrocarbon remnants of eukaryotic C 26-C 30 sterols can be used to reconstruct early algal evolution. Enhanced C 29 sterol abundances provide algal cell membranes a density advantage in large temperature fluctuations. Here, we combined a literature review with new analyses to generate a comprehensive inventory of unambiguously syngenetic steranes in Neoproterozoic rocks. Our results show that the capacity for C 29 24ethyl-sterol biosynthesis emerged in the Cryogenian, that is, between 720 and 635 million years ago during the Neoproterozoic Snowball Earth glaciations, which were an evolutionary stimulant, not a bottleneck. This biochemical innovation heralded the rise of green algae to global dominance of marine ecosystems and highlights the environmental drivers for the evolution of sterol biosynthesis. The Cryogenian emergence of C 29 sterol biosynthesis places a benchmark for verifying older sterane signatures and sets a new framework for our understanding of early algal evolution