Impact of ripening stage and drying on selected quality attributes of apple mango cubes and leathers

Apple mango is an improved cultivar that has been widely adopted by farmers in Kenya for use in the fresh market and processing. However, its production and consumption are adversely affected by high postharvest losses, which result from the perishable nature of the fruit, especially during glut periods. This is partly due to limited availability of information on alternative product use. The aim of this study was to evaluate the impact of ripening stage and drying on the physico-chemical quality and sensory acceptability of Apple mango fruit grown in the upper Athi River region of Kenya. Moisture, crude ash, crude fibre, titratable acidity, total soluble solids, colour, total carotenoids and ascorbic acid of the unripe and ripe fresh mangoes were determined. Subsequent experiments involved drying of ripe and unripe mango cubes (8mm) in a windy oven (at 60°C) and, in parallel, drying of ripe mango puree (mixed with sucrose-glucose solution, citric acid and pectin) in a windy oven at three different temperatures (50, 60 and 70°C) which resulted in mango leathers. Analysis of total carotenoids, ascorbic acid content and colour was done for all dried samples, followed by sensory evaluation using the nine-point hedonic scale. The moisture content, crude ash and total soluble solids increased significantly (P<0.05) with ripening while titratable acidity decreased significantly (P<0.05). Ascorbic acid content decreased from 98.03 to 86.45 mg/100g with ripening while total carotenoids content approximately doubled from 768 to 1436 μg/100g. Drying resulted in high retention of total carotenoids, whereas, ascorbic acid content decreased. The mango samples became darker and redder in all cases. Dried mango cubes and leathers derived from ripe mangoes had higher scores in the sensory analysis compared to those obtained from unripe mangoes. In conclusion, the stage of ripening and drying technique employed are critical in determining the nutritional and sensory characteristics of dried Apple mango cubes and leathers. Drying Apple mango leathers at 60°C is the best method that can be adopted.Keywords: Apple Mango, Drying, Ripening, Fruit Leather, Fruit Cubes, Physico-chemical, Sensor

Effect of improved stoves on prevalence of acute respiration infection and conjuctivitis among children and women in a rral community in Kenya

Objectives: To estimate the effect of improved stoves on the prevalence of ARI and conjunctivitis among children aged below five years and women aged between 15 and 60 years.Method: A field trial or intervention study design, in which cluster and random sampling were used to recruit households with improved stoves. Nearest households with traditional three-stone stoves were recruited as controls. Prevalence of ARI and conjunctivitis among the study subjects was estimated by clinical evaluation and physicalobservation, and by history (including clinical and socioeconomic information), and compared between the two study groups.Results: The prevalence of ARI among children aged below five years and among women aged between 15 and 60 years were significantly higher in households with the traditional three-stone stoves than in those with the improved stove c2 =31.45 r = 0.00000, relativerisk = 2.6, C.l. 1.86,3.63, and c2 = 30. 13, r = 0.00000, Relative Risk = 2.8, C.l. 1.93,4.06, respectively). Similarly the prevalence of conjunctivitis among children aged below five years and among women aged between 15 and 60 years were significantly higher in households with traditional three-stone stoves than in those with the improved stoves (c2 = 24.18, p = 0.00000, Relative Risk = 3.3, C.l. 2.05,5.32, and c2 = 7.6, r = 0.0057, Relative Risk = 3, C.I. 1.38, 6.54. respectively).Conclusion: Prevalence of ARI and conjunctivitis among children aged below five years and also among women aged between 15 and 60 years in households with the traditional three-stone stoves was significantly higher than that in households with improved stoves

Why do women choose private over public facilities for family planning services? A qualitative study of post-partum women in an informal urban settlement in Kenya

BACKGROUND: Nearly 40 % of women in developing countries seek contraceptives services from the private sector. However, the reasons that contraceptive clients choose private or public providers are not well studied. METHODS: We conducted six focus groups discussions and 51 in-depth interviews with postpartum women (n = 61) to explore decision-making about contraceptive use after delivery, including facility choice. RESULTS: When seeking contraceptive services, women in this study preferred private over public facilities due to convenience and timeliness of services. Women avoided public facilities due to long waits and disrespectful providers. Study participants reported, however, that they felt more confident about the technical medical quality in public facilities than in private, and believed that private providers prioritized profit over safe medical practice. Women reported that public facilities offered comprehensive counseling and chose these facilities when they needed contraceptive decision-support. Provision of comprehensive counseling and screening, including side effects counseling and management, determined perception of quality. CONCLUSION: Women believed private providers offered the advantages of convenience, efficiency and privacy, though they did not consistently offer high-quality care. Quality-improvement of contraceptive care at private facilities could include technical standardization and accreditation. Development of support and training for side effect management may be an important intervention to improve perceived quality of care

Persistent anthrax as a major driver of wildlife mortality in a tropical rainforest

Anthrax is a globally important animal disease and zoonosis. Despite this, our current knowledge of anthrax ecology is largely limited to arid ecosystems, where outbreaks are most commonly reported. Here we show that the dynamics of an anthrax-causing agent, Bacillus cereus biovar anthracis, in a tropical rainforest have severe consequences for local wildlife communities. Using data and samples collected over three decades, we show that rainforest anthrax is a persistent and widespread cause of death for a broad range of mammalian hosts. We predict that this pathogen will accelerate the decline and possibly result in the extirpation of local chimpanzee (Pan troglodytes verus) populations. We present the epidemiology of a cryptic pathogen and show that its presence has important implications for conservation

Delivering medical abortion at scale: a study of the retail market for medical abortion in Madhya Pradesh, India.

BACKGROUND: Medical abortion (mifepristone and misoprostol) has the potential to contribute to reduced maternal mortality but little is known about the provision or quality of advice for medical abortion through the private retail sector. We examined the availability of medical abortion and the practices of pharmacists in India, where abortion has been legal since 1972. METHODS: We interviewed 591 pharmacists in 60 local markets in city, town and rural areas of Madhya Pradesh. One month later, we returned to 359 pharmacists with undercover patients who presented themselves unannounced as genuine customers seeking a medical abortion. RESULTS: Medical abortion was offered to undercover patients by 256 (71.3%) pharmacists and 24 different brands were identified. Two thirds (68.5%) of pharmacists stated that abortion was illegal in India. Only 106 (38.5%) pharmacists asked clients the timing of the last menstrual period and 38 (13.8%) requested to see a doctor's prescription - a legal requirement in India. Only 59 (21.5%) pharmacists correctly advised patients on the gestational limit for medical abortion, 97 (35.3%) provided correct information on how many and when to take the tablets in a combination pack, and 78 (28.4%) gave accurate advice on where to seek care in case of complications. Advice on post-abortion family planning was almost nonexistent. CONCLUSIONS: The retail market for medical abortion is extensive, but the quality of advice given to patients is poor. Although the contribution of medical abortion to women's health in India is poorly understood, there is an urgent need to improve the practices of pharmacists selling medical abortion

One thousand plant transcriptomes and the phylogenomics of green plants

Abstract: Green plants (Viridiplantae) include around 450,000–500,000 species1, 2 of great diversity and have important roles in terrestrial and aquatic ecosystems. Here, as part of the One Thousand Plant Transcriptomes Initiative, we sequenced the vegetative transcriptomes of 1,124 species that span the diversity of plants in a broad sense (Archaeplastida), including green plants (Viridiplantae), glaucophytes (Glaucophyta) and red algae (Rhodophyta). Our analysis provides a robust phylogenomic framework for examining the evolution of green plants. Most inferred species relationships are well supported across multiple species tree and supermatrix analyses, but discordance among plastid and nuclear gene trees at a few important nodes highlights the complexity of plant genome evolution, including polyploidy, periods of rapid speciation, and extinction. Incomplete sorting of ancestral variation, polyploidization and massive expansions of gene families punctuate the evolutionary history of green plants. Notably, we find that large expansions of gene families preceded the origins of green plants, land plants and vascular plants, whereas whole-genome duplications are inferred to have occurred repeatedly throughout the evolution of flowering plants and ferns. The increasing availability of high-quality plant genome sequences and advances in functional genomics are enabling research on genome evolution across the green tree of life

Examining characteristics, knowledge and regulatory practices of specialized drug shops in Sub-Saharan Africa: a systematic review of the literature.

BACKGROUND: Specialized drug shops such as pharmacies and drug shops are increasingly becoming important sources of treatment. However, knowledge on their regulatory performance is scarce. We set out to systematically review literature on the characteristics, knowledge and practices of specialized drug shops in Sub-Saharan Africa. METHODS: We searched PubMed, EMBASE, WEB of Science, CAB Abstracts, PsycINFO and websites for organizations that support medicine policies and usage. We also conducted open searches using Google Scholar, and searched manually through references of retrieved articles. Our search included studies of all designs that described characteristics, knowledge and practices of specialized drug shops. Information was abstracted on authors, publication year, country and location, study design, sample size, outcomes investigated, and primary findings using a uniform checklist. Finally, we conducted a structured narrative synthesis of the main findings. RESULTS: We obtained 61 studies, mostly from Eastern Africa, majority of which were conducted between 2006 and 2011. Outcome measures were heterogeneous and included knowledge, characteristics, and dispensing and regulatory practices. Shop location and client demand were found to strongly influence dispensing practices. Whereas shops located in urban and affluent areas were more likely to provide correct treatments, those in rural areas provided credit facilities more readily. However, the latter also charged higher prices for medicines. A vast majority of shops simply sold whatever medicines clients requested, with little history taking and counseling. Most shops also stocked popular medicines at the expense of policy recommended treatments. Treatment policies were poorly communicated overall, which partly explained why staff had poor knowledge on key aspects of treatment such as medicine dosage and side effects. Overall, very little is known on the link between regulatory enforcement and practices of specialized drug shops. CONCLUSIONS: Evidence suggests that characteristics and practices of specialized drug shops differ across rural and urban locations, and that these providers are highly responsive to client demand. However, there is a dearth in knowledge on how regulatory enforcement influences their characteristics and practices, and what strategies can be employed to strengthen the governance of the retail pharmaceutical sector