42 research outputs found
Hexavalent Chromium Reduction under Fermentative Conditions with Lactate Stimulated Native Microbial Communities
- Author
- Adam P. Arkin
- AH Alvarez
- Anil C. Somenahally
- Anthony V. Palumbo
- AS Madden
- B Faybishenko
- B Faybishenko
- BH Gu
- C Cervantes
- C Quince
- CB Walker
- CL Zhang
- CS Sheik
- DA Elias
- DA Elias
- DR Lovley
- Dwayne A. Elias
- E Burkhardt
- E Lira-Silva
- EL Brodie
- H Abken
- HR Beller
- Jennifer J. Mosher
- JG Caporaso
- Jizhong Zhou
- JJ Mosher
- JJ Mosher
- JM Senko
- Joy D. Van Nostrand
- KH Nealson
- M Shakya
- MI Ramírez-Díaz
- Mircea Podar
- NM Dogan
- PD Schloss
- PJ Weimer
- PP Chaudhary
- R Bencheikh-Latmani
- R Jasso-Chavez
- Rafael Moreno-Sanchez
- SS Hubbard
- Steven D. Brown
- TA Vishnivetskaya
- Terry C. Hazen
- TM Gihring
- Tommy J. Phelps
- Tong Yuan
- W Kühn
- Z Lu
- Zamin K. Yang
- Ø Hammer
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/01/2013
- Field of study
This work conducted by ENIGMA- Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory. The submitted manuscript has been authored by a contractor of the U.S. Government under contract DE-AC05-00OR22725. Accordingly, the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.Conceived and designed the experiments: ACS JJM MP TJP SDB AVP DAE. Performed the experiments: ACS JJM ZKY. Analyzed the data: ACS JJM TY JDVN JZ DAE. Contributed reagents/materials/analysis tools: DAE TCH APA. Wrote the paper: ACS JJM DAE.Microbial reduction of toxic hexavalent chromium (Cr(VI)) in-situ is a plausible bioremediation strategy in electron-acceptor limited environments. However, higher Cr(VI) may impose stress on syntrophic communities and impact community structure and function. The study objectives were to understand the impacts of Cr(VI) concentrations on community structure and on the Cr(VI)-reduction potential of groundwater communities at Hanford, WA. Steady state continuous flow bioreactors were used to grow native communities enriched with lactate (30 mM) and continuously amended with Cr(VI) at 0.0 (No-Cr), 0.1 (Low-Cr) and 3.0 (High-Cr) mg/L. Microbial growth, metabolites, Cr(VI), 16S rRNA gene sequences and GeoChip based functional gene composition were monitored for 15 weeks. Temporal trends and differences in growth, metabolite profiles, and community composition were observed, largely between Low-Cr and High-Cr bioreactors. In both High-Cr and Low-Cr bioreactors, Cr(VI) levels were below detection from week 1 until week 15. With lactate enrichment, native bacterial diversity substantially decreased as Pelosinus spp., and Sporotalea spp., became the dominant groups, but did not significantly differ between Cr concentrations. The Archaea diversity also substantially decreased after lactate enrichment from Methanosaeta (35%), Methanosarcina (17%) and others, to mostly Methanosarcina spp. (95%). Methane production was lower in High-Cr reactors suggesting some inhibition of methanogens. Several key functional genes were distinct in Low-Cr bioreactors compared to High-Cr. Among the Cr resistant microbes, Burkholderia vietnamiensis, Comamonas testosterone and Ralstonia pickettii proliferated in Cr amended bioreactors. In-situ fermentative conditions facilitated Cr(VI) reduction, and as a result 3.0 mg/L Cr(VI) did not impact the overall bacterial community structure.Yeshttp://www.plosone.org/static/editorial#pee
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- Publication venue
- 'American Institute of Mathematical Sciences (AIMS)'
- Publication date
- 01/01/2016
- Field of study
Natural polymers, such as gums and mucilage, are biocompatible, cheap, easily available and non-toxic materials of native origin. These polymers are increasingly preferred over synthetic materials for industrial applications due to their intrinsic properties, as well as they are considered alternative sources of raw materials since they present characteristics of sustainability, biodegradability and biosafety. As definition, gums and mucilages are polysaccharides or complex carbohydrates consisting of one or more monosaccharides or their derivatives linked in bewildering variety of linkages and structures. Natural gums are considered polysaccharides naturally occurring in varieties of plant seeds and exudates, tree or shrub exudates, seaweed extracts, fungi, bacteria, and animal sources. Water-soluble gums, also known as hydrocolloids, are considered exudates and are pathological products; therefore, they do not form a part of cell wall. On the other hand, mucilages are part of cell and physiological products. It is important to highlight that gums represent the largest amounts of polymer materials derived from plants. Gums have enormously large and broad applications in both food and non-food industries, being commonly used as thickening, binding, emulsifying, suspending, stabilizing agents and matrices for drug release in pharmaceutical and cosmetic industries. In the food industry, their gelling properties and the ability to mold edible films and coatings are extensively studied. The use of gums depends on the intrinsic properties that they provide, often at costs below those of synthetic polymers. For upgrading the value of gums, they are being processed into various forms, including the most recent nanomaterials, for various biotechnological applications. Thus, the main natural polymers including galactomannans, cellulose, chitin, agar, carrageenan, alginate, cashew gum, pectin and starch, in addition to the current researches about them are reviewed in this article.. }To the Conselho Nacional de Desenvolvimento Cientfíico e Tecnológico (CNPq) for fellowships (LCBBC and MGCC) and the Coordenação de Aperfeiçoamento de Pessoal de Nvíel Superior (CAPES) (PBSA). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and COMPETE 2020 (POCI-01-0145-FEDER-006684) (JAT)
A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)
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- AKI Research Group
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- Champigneulle B
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- Chan KC
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- Charron C
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- Cheng CC
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- Chernyshuk S
- Chevret S
- Chhor V
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- Chiappa C
- Chiou KR
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- Chiu LC
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- Chiurazzi C
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- Cho Y
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- Chok L
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- Cholley B
- Chondropoulos S
- Chou W
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- Clavel M
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- Clermont G
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- Colaprico A
- Colino L
- Collange O
- Colombo A
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- Colson P
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- Constan A
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- Cordeiro CP
- Cordero M
- Cordolcini V
- Cordovilla-Guardia S
- Cordón J
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- de Hoyos EA
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- de la Fuente MV
- De La Fuente-Martos C
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- De Pascale G
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- de Wilde W
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- Deblier I
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- Di Gravio V
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- Elbers PW
- Elgendy M
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- Engelberts D
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- Esteban A
- Estella A
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- Fernández-Ortega JF
- Ferraz AB
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- Ferri F
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- Fiks T
- Fiks T
- Fileković S
- Filho CA
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- FINNRESUSCI Study Group
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- Fleischmann E
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- Frithiof R
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- Georgopoulos D
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- GETGAG Working Group
- GETGAG/SEMICYUC
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- Hernández A
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- Hernández AA
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- Hernández-Tejedor A
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- Hutchings S
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- Ibsen M
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- Ignacio ML
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- Ilyina V
- Imanaka H
- Inaloo S
- Ince C
- Iotti GA
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- IPREA Study Group
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- Japan Septic Disseminated Intravascular Coagulation (JSEPTIC DIC) study group
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- Jayasinghe S
- Jean-Baptiste S
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- Jensen AE
- Jensen JU
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- JSEPTIC (Japanese Society of Education for Physicians and Trainees in Intensive Care) Clinical Trial Group
- Juan WC
- Juanas CA
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- Kulkarni AP
- Kumari BK
- Kumbamu A
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- Lin KC
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- Lischinsky A
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- Lobo-Civico A
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- Lombardo MD
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- Maistry N
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- Martínez-Carmona JF
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- Merino-Vega D
- Messenger D
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- Midwinter MJ
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- Miguelena PR
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- Montenegro AP
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- Plug Working Group
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- PS-ICU Group
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- Rapid Diagnosis of Infections in the Critically Ill Team
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- Rosario LE
- Rosario LE
- Rosario LE
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- Rosario LE
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- Ruiz FC
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- Ruiz-Rodriguez JC
- Ruiz-Rodríguez JC
- Ruiz-Ruano RDEL
- Runge I
- Runge I
- Runge I
- Rutherford WB
- Rutten AM
- Rydingsward JE
- Saadat F
- Sabetian G
- Sabeļņikovs O
- Sabirov D
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- Santana MC
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- Santana PS
- Santana PS
- Santana SR
- Santana SR
- Santiago AI
- Santiago AI
- Santos F
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- Sanz ER
- Sanz ER
- Sanz ER
- Sanz ER
- Sanz ER
- Sanz ER
- Sanz NM
- Sanz NM
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- Savin I
- Savvidou S
- Sawamura A
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- Scales DC
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- Schneider AG
- Schoenenberger JA
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- Spatenkova V
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- Spronk PE
- Spronk PE
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- St George’s University of London
- Stamm J
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- Stubbs C
- Student Research Committee - Shiraz University of Medical Sciences
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- Su L
- Su PL
- Su PL
- Suchomel P
- Suh GJ
- Suh GJ
- Suh GJ
- Suh GY
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- Sun XM
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- Sánchez B
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- Sánchez MJ
- Sánchez MJ
- Sánchez MJ
- Sánchez MJ
- Sánchez MJ
- Sánchez MJ
- Sánchez PC
- Sánchez PC
- Sánchez-Elvira LA
- Tabatabaei HR
- Tabei SH
- Taccone F
- Taccone FS
- Taccone FS
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- Torrance HD
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- Villarreal E
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- Welters I
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- Wijayatilake DS
- Wildenberg L
- Wilks M
- Wilson ME
- WIND study group
- WIND study group
- WIND study group
- Winder-Rhodes S
- Witter T
- Wolf M
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- Wong Y
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- Yamaguchi Y
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- Yamazaki A
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- Yılmaz G
- Yilmazer F
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- Yonis H
- Yonis H
- Yonis H
- Yoon SZ
- Yoruk F
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- Yoshida T
- Yoshida T
- Yoshihiro S
- Yoshitake E
- You KM
- Youn AM
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- Zýková I
- Álvarez JT
- Álvarez-Lerma F
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2016
- Field of study
Meeting abstrac
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
- Abdoli A
- Abel S
- Abeliovich H
- Abildgaard MH
- Abudu YP
- Acevedo-Arozena A
- Adamopoulos IE
- Adeli K
- Adolph TE
- Adornetto A
- Aflaki E
- Agam G
- Agarwal A
- Aggarwal BB
- Agnello M
- Agostinis P
- Agrewala JN
- Agrotis A
- Aguilar PV
- Ahmad ST
- Ahmed ZM
- Ahumada-Castro U
- Aits S
- Aizawa S
- Akkoc Y
- Akoumianaki T
- Akpinar HA
- Al-Abd AM
- Al-Akra L
- Al-Gharaibeh A
- Alaoui-Jamali MA
- Alberti S
- Alcocer-Gómez E
- Alessandri C
- Ali M
- Alim Al-Bari MA
- Aliwaini S
- Alizadeh J
- Almacellas E
- Almasan A
- Alonso A
- Alonso GD
- Altan-Bonnet N
- Altieri DC
- Alves da Costa C
- Alves S
- Alzaharna MM
- Amadio M
- Amantini C
- Amaral C
- Ambrosio S
- Amer AO
- Ammanathan V
- An Z
- Andersen SU
- Andrabi SA
- Andrade-Silva M
- Andres AM
- Angelini S
- Ann D
- Anozie UC
- Ansari MY
- Antas P
- Antebi A
- Antón Z
- Anwar T
- Apetoh L
- Apostolova N
- Araki T
- Araki Y
- Arasaki K
- Araya J
- Araújo WL
- Arden C
- Arguelles S
- Arias E
- Arikkath J
- Arimoto H
- Ariosa AR
- Armstrong-James D
- Arnauné-Pelloquin L
- Aroca A
- Arroyo DS
- Arsov I
- Artero R
- Arévalo M-A
- Asaro DML
- Aschner M
- Ashrafizadeh M
- Ashur-Fabian O
- Atanasov AG
- Au AK
- Auberger P
- Auner HW
- Aurelian L
- Autelli R
- Avagliano L
- Aveic S
- Aveleira CA
- Avin-Wittenberg T
- Aydin Y
- Ayton S
- Ayyadevara S
- Azzopardi M
- Baba M
- Backer JM
- Backues SK
- Bae D-H
- Bae O-N
- Bae SH
- Baehrecke EH
- Baek A
- Baek S-H
- Baek SH
- Bagetta G
- Bagniewska-Zadworna A
- Bai H
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- Ávalos Y
- Önal G
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- Čolić M
- Đokić J
- Žerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Evaluation of appendicitis risk prediction models in adults with suspected appendicitis
- Author
- Abbas Sh
- Abbas Sh
- Abbassi Oa
- Abbott T
- Abdelgadir Am
- Abdelrahman A
- Abdelrahman M
- Abdelrahman M
- Abdelwahed A
- Abellan M
- Abellán Am
- Abellán Am
- Abulafi M
- Acharya A
- Acosta A
- Adam Me
- Adams Re
- Adegbola So
- Adegbola So
- Adimonye A
- Adnan M
- Afshar S
- Agresta F
- Agua Ia
- Aguayo Jl
- Agudo Ar
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- Ahel J
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- Ahmad A
- Ahmed B
- Ahmed G
- Ahmed Os
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- Ainsworth P
- Ais G
- Ais G
- Aisoni F
- Akbari K
- Akhtar K
- Akinsola O
- Akram F
- Al-Faham Z
- Al-Khafaji N
- Al-Khyatt W
- Al-Musawi S
- Al-Sarireh B
- Al-Sheikh M
- Alagna V
- Alani M
- Alberca-Paramo A
- Aldrey I
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- Alhammali T
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- Allington J
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- Amayo A
- Ambrona-Zafra D
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- Watson N
- Wauchope J
- Weatherburn Lw
- Weaver J
- Weegenaar Cr
- Weegenaar Cr
- Welsh S
- Wensley F
- West H
- West H
- Wheatstone S
- Wheeler C
- Whewell He
- White F
- Whitehorn Se
- Whitehorn Se
- Whitehouse P
- Whiteman E
- Whittaker L
- Wiggill S
- Wijesundera K
- Wilcox G
- Wilkin R
- Wilkin R
- Wilkin R
- Wilkin R
- Wilkin R. J. W.
- Williams Gl
- Williams M
- Williams R
- Williams S
- Wilson Ej
- Wilson M
- Winter Dc
- Winter Dc
- Wolff J
- Wong A
- Wong C
- Wong J
- Wong J
- Wong Ml
- Wong Sy
- Woo R
- Wood Cs
- Woodrow C
- Woodward A
- Woodward B
- Woodward B
- Worku D
- Worku D
- Wright E
- Wright Hl
- Wu F
- Xidas A
- Yalamarthi S
- Yang P
- Yanni F
- Yardimci E
- Yasin T
- Yen Sk
- Yeung K
- Yeung K
- Yoganathan S
- Yoong S
- Youssef H
- Yow L
- Yow L
- Zaborowski A
- Zadi Az
- Zalla T
- Zarka Za
- Zarka Za
- Zarog Ma
- Zelazek M
- Zerpa C
- Zhang Ay
- Zhou S
- Zorrilla L
- Zuin M
- Zurleni Tz
- Publication venue
- 'Wiley'
- Publication date
- 01/01/2019
- Field of study
Background
Appendicitis is the most common general surgical emergency worldwide, but its diagnosis remains challenging. The aim of this study was to determine whether existing risk prediction models can reliably identify patients presenting to hospital in the UK with acute right iliac fossa (RIF) pain who are at low risk of appendicitis.
Methods
A systematic search was completed to identify all existing appendicitis risk prediction models. Models were validated using UK data from an international prospective cohort study that captured consecutive patients aged 16–45 years presenting to hospital with acute RIF in March to June 2017. The main outcome was best achievable model specificity (proportion of patients who did not have appendicitis correctly classified as low risk) whilst maintaining a failure rate below 5 per cent (proportion of patients identified as low risk who actually had appendicitis).
Results
Some 5345 patients across 154 UK hospitals were identified, of which two‐thirds (3613 of 5345, 67·6 per cent) were women. Women were more than twice as likely to undergo surgery with removal of a histologically normal appendix (272 of 964, 28·2 per cent) than men (120 of 993, 12·1 per cent) (relative risk 2·33, 95 per cent c.i. 1·92 to 2·84; P < 0·001). Of 15 validated risk prediction models, the Adult Appendicitis Score performed best (cut‐off score 8 or less, specificity 63·1 per cent, failure rate 3·7 per cent). The Appendicitis Inflammatory Response Score performed best for men (cut‐off score 2 or less, specificity 24·7 per cent, failure rate 2·4 per cent).
Conclusion
Women in the UK had a disproportionate risk of admission without surgical intervention and had high rates of normal appendicectomy. Risk prediction models to support shared decision‐making by identifying adults in the UK at low risk of appendicitis were identified
chr genes from adaptive replicons are responsible for chromate resistance by Burkholderia xenovorans LB400
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Effectiveness of natural protected areas for preventing land use and land cover changes of the Transmexican Volcanic Belt, Mexico
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Computation of influence functions for automatic mining subsidence prediction
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Isolation and Characterization of Chromium(VI)-Reducing Bacteria from Tannery Effluents
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Virulence Conferred by PumA Toxin from the Plasmid-Encoded PumAB Toxin-Antitoxin System is Regulated by Quorum System
- Author
- B Kedzierska
- BA Lazazzera
- CS Pereira
- D Erdönmez
- E Rodríguez-Andrade
- G Boşgelmez-Tinaz
- J Lee
- J Quick
- JN Pendleton
- JP Pearson
- KC Hernández-Ramírez
- L Fernández-García
- LCM Antunes
- LG Rahme
- M Schuster
- M Schuster
- MA De la Cruz
- MI Ramírez-Díaz
- MR Green
- MW Pfaffl
- NA Whitehead
- R Leplae
- R Page
- S Brenner
- S Hemati
- SE Battle
- SJ Unterholzner
- WL Ng
- X Dong
- X Wang
- Y Deng
- Y Otsuka
- Y Wen
- Y Yamaguchi
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study