CA 125 is a better marker to differentiate endometrial cancer and abnormal uterine bleeding

Background: Incidence of endometrial cancer in India is increasing due to lifestyle changes and obesity. As 5 year survival rate of cancer confined to uterus is good, there is need for serum tumor marker for early diagnosis. This study was designed to identify a tumor marker which differentiate endometrial carcinoma and abnormal uterine bleeding (AUB) because common presentation of endometrial carcinoma is AUB. Objectives: To estimate and compare serum prolactin, Cancer Antigen 125 (CA-125), Cancer Antigen 15-3 (CA15-3), and Carcino embryonic antigen (CEA) levels in patients with endometrial cancer and abnormal uterine bleeding; To evaluate the role of these markers in diagnosing endometrial cancer. Methodology: Thirty eight patients with endometrial cancer and 40 patients with AUB were recruited in this study. Serum prolactin, CA 125, CEA, and CA 15-3 levels were estimated in both groups. Results: The levels of CA 15-3, CA 125, CEA, and prolactin were increased in endometrial carcinoma patients, on comparison with AUB patients. CA 125 alone was found to be a better marker to detect endometrial cancer with 52.63% sensitivity, 80.00% specificity. Conclusion: As individual tumor marker, serum CA 125 has the ability to detect endometrial cancer in patients with abnormal uterine bleeding

An RF Approach to Modelling Gallium Nitride Power Devices Using Parasitic Extraction

This paper begins with a comprehensive review into the existing GaN device models. Secondly, it identifies the need for a more accurate GaN switching model. A simple practical process based on radio frequency techniques using Vector Network Analyser is introduced in this paper as an original contribution. It was applied to extract the impedances of the GaN device to develop an efficient behavioural model. The switching behaviour of the model was validated using both simulation and real time double pulse test experiments at 500 V, 15 A conditions. The proposed model is much easier for power designers to handle, without the need for knowledge about the physics or geometry of the device. The proposed model for Transphorm GaN HEMT was found to be 95.2% more accurate when compared to the existing LT-Spice manufacturer model. This work additionally highlights the need to adopt established RF techniques into power electronics to reduce the learning curve while dealing with these novel high-speed switching devices.</jats:p

Implementation of Non-Isolated Zeta-KY Triple Port Converter for Renewable Energy Applications

This paper proposes a three-port Zeta-KY dc-dc converter which is fed with hybrid sources like photovoltaic (PV) cells and batteries. The converter proposed here is a multi-input single-output (MISO) structure which harnesses the benefits of Zeta and KY converters. The combination of these converters is highly advantageous since the Zeta converter provides lesser output voltage ripples with high gain and the KY converter topology suits well for withstanding load transients. The KY converter used in this research work is subjected to a topological change to facilitate bidirectional power flow. The bidirectional flow is essential to save the excess power in PV source in batteries during low load conditions. This novel multiport topology with bidirectional facility is first of its kind and has not been discussed earlier in the research arena. In the proposed work, two control algorithms are developed and deployed: the first one ensures the maximum power extraction from the PV and the second one maintains constant dc bus voltage and manages bidirectional power flow. MATLAB Simulink and hardware prototype of the proposed system has been realized for a 72 V dc bus and a 500 W electric vehicular drive. The simulation and experimental results reveal that the proposed system is viable for medium power electric shuttle applications. The proposed system is subjected to various test cases and it is observed that the source and load intermittencies are catered very well by the proposed three port Zeta-KY converter. The developed multiport converter is feasible for renewable energy applications

Indirect Effective Controlled Split Source Inverter-Based Parallel Active Power Filter for Enhancing Power Quality

The existing solutions for reducing total harmonic distortion (THD) using different control algorithms in shunt active power filters (SAPFs) are complex. This work proposes a split source inverter (SSI)-based SAPF for improving the power quality in a nonlinear load system. The advantage of the SSI topology is that it is of a single stage boost inverter with an inductor and capacitor where the conventional two stages with an intermediate DC-DC conversion stage is discarded. This research proposes inventive control schemes for SAPF having two control loops; the outer control loop regulates the DC link voltage whereas the inner current loop shapes the source current profile. The control mechanism implemented here is an effective, less complex, indirect scheme compared to the existing time domain control algorithms. Here, an intelligent fuzzy logic control regulates the DC link voltage which facilitates reference current generation for the current control scheme. The simulation of the said system was carried out in a MATLAB/Simulink environment. The simulations were carried out for different load conditions (RL and RC) using a fuzzy logic controller (FLC) and PI controllers in the outer loop (voltage control) and hysteresis current controller (HCC) and sinusoidal pulse width modulation (SPWM) in the inner loop (current control). The simulation results were extracted for dynamic load conditions and the results demonstrated that the THD can be reduced to 0.76% using a combination of SPWM and FLC. Therefore, the proposed system proved to be effective and viable for reducing THD. This system would be highly applicable for renewable energy power generation such as Photovoltaic (PV) and Fuel cell (FC)

Performance Enhancement of a Partially Shaded Photovoltaic Array by Optimal Reconfiguration and Current Injection Schemes

The output of a photovoltaic array is reduced considerably when PV panels are shaded even partially. The impact of shading causes an appreciable loss in power delivery, since the PV panels are connected in series and parallel to contribute to the required voltage and power for the load. The prevailing research on mitigating the shading impact is mostly based on complex reconfiguration strategies where the PV panels are subjected to complex rewiring schemes. On the other hand, to disperse the shading many studies in the literature defend the physical rearrangement of the panels. The available intensive reconfiguration schemes, such as the series parallel (SP), bridge link (BL), honeycomb (HC), and total cross tied (TCT) schemes, try only to mitigate the shading impact and there is no scope for compensation; as a result, a loss of output power is inevitable. In the proposed research work, both the mitigation of and the compensation for the losses incurred due to shading are studied. In this work, an optimal reconfiguration scheme is adopted to reduce the shading impact and a power electronic circuit with a battery source is designed to compensate for the shading losses in all aspects. In the optimal reconfiguration scheme, a bifurcation strategy is adopted in each column and the electrical connections of the PV panels are interchanged such that the shading impact is dispersed. The power electronic circuit consists of a half-bridge buck converter with a battery source that injects the current required by a shaded column. This setup compensates for the shaded PV array’s power and improves the efficiency of the total system. The proposed scheme was implemented in a 3200 W system and subjected to various shading patterns, including single panel shading, corner shading, long and wide shading, and random shading. The proposed scheme was simulated in the MATLAB Simulink environment and compared with static 4 × 4 PV array configurations, including the series parallel (SP), bridge link (BL), honeycomb (HC), and total cross tied (TCT) configurations. The comparative performance was assessed in terms of mismatch power loss, fill factor, and efficiency. The proposed system is suitable for all shading patterns and was proved to be very efficient even in the worst shading, where 1353 W was saved

Social Grouping Algorithm Aided Maximum Power Point Tracking Scheme for Partial Shaded Photovoltaic Array

Photovoltaic (PV) systems-based energy generation is relatively easy to install, even at a large scale, because it is scalable in size and is thus easy to transport. Harnessing maximum power is only possible if maximum power tracking (MPPT) functionality is available as part of the power converter control that interfaces the PV panels to the grid. Solar exposure covering all PV panels is unlikely to happen all the time, which is known as a partial shading (PS) phenomenon. As a result, depending on the MPPT algorithm adopted, it may fail to find a maximum global power peak, being locked into a local power peak. This research work discusses an alternative MPPT control technique inspired in the social group optimization (SGO) algorithm. SGO belongs to the meta-heuristic optimization techniques family. In this sense, the SGO method ability for solving global optimization problems is explored to find the global maximum power point (GMPP) under the presence of local MPPs. The introduced SGO–MPPT was subjected to different PS conditions and complex shading patterns. Then, its performance was compared to other global search MPPT techniques, which include particle swarm optimization (PSO), the dragon fly algorithm (DFO) and the artificial bee colony algorithm (ABC). The simulation outcomes for the SGO–MPPT characterization showed good results, namely rapid global power tracking in less than 0.2 s with reduced oscillation; the efficiency of solar energy harness was slightly above 99%

Enhanced AGAMOUS expression in the centre of the Arabidopsis flower causes ectopic expression over its outer expression boundaries

Spatial regulation of C-function genes controlling reproductive organ identity in the centre of the flower can be achieved by adjusting the level of their expression within the genuine central expression domain in Antirrhinum and Petunia. Loss of this control in mutants is revealed by enhanced C-gene expression in the centre and by lateral expansion of the C-domain. In order to test whether the level of central C-gene expression and hence the principle of ‘regulation by tuning’ also applies to spatial regulation of the C-function gene AGAMOUS (AG) in Arabidopsis, we generated transgenic plants with enhanced central AG expression by using stem cell-specific CLAVATA3 (CLV3) regulatory sequences to drive transcription of the AG cDNA. The youngest terminal flowers on inflorescences of CLV3::AG plants displayed homeotic features in their outer whorls indicating ectopic AG expression. Dependence of the homeotic feature on the age of the plant is attributed to the known overall weakening of repressive mechanisms controlling AG. Monitoring AG with an AG-I::GUS reporter construct suggests ectopic AG expression in CLV3::AG flowers when AG in the inflorescence is still repressed, although in terminating inflorescence meristems, AG expression expands to all tissues. Supported by genetic tests, we conclude that upon enhanced central AG expression, the C-domain laterally expands necessitating tuning of the expression level of C-function genes in the wild type. The tuning mechanism in C-gene regulation in Arabidopsis is discussed as a late security switch that ensures wild-type C-domain control when other repressive mechanism starts to fade and fail

Microdevices for extensional rheometry of low viscosity elastic liquids : a review

Extensional flows and the underlying stability/instability mechanisms are of extreme relevance to the efficient operation of inkjet printing, coating processes and drug delivery systems, as well as for the generation of micro droplets. The development of an extensional rheometer to characterize the extensional properties of low viscosity fluids has therefore stimulated great interest of researchers, particularly in the last decade. Microfluidics has proven to be an extraordinary working platform and different configurations of potential extensional microrheometers have been proposed. In this review, we present an overview of several successful designs, together with a critical assessment of their capabilities and limitations

Chromosomal-level assembly of the Asian Seabass genome using long sequence reads and multi-layered scaffolding

We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. SNP analyses identified high levels of genetic diversity and confirmed earlier indications of a population stratification comprising three clades with signs of admixture apparent in the South-East Asian population. The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics

Expression and Cellular Immunogenicity of a Transgenic Antigen Driven by Endogenous Poxviral Early Promoters at Their Authentic Loci in MVA

CD8+ T cell responses to vaccinia virus are directed almost exclusively against early gene products. The attenuated strain modified vaccinia virus Ankara (MVA) is under evaluation in clinical trials of new vaccines designed to elicit cellular immune responses against pathogens including Plasmodium spp., M. tuberculosis and HIV-1. All of these recombinant MVAs (rMVA) utilize the well-established method of linking the gene of interest to a cloned poxviral promoter prior to insertion into the viral genome at a suitable locus by homologous recombination in infected cells. Using BAC recombineering, we show that potent early promoters that drive expression of non-functional or non-essential MVA open reading frames (ORFs) can be harnessed for immunogenic expression of recombinant antigen. Precise replacement of the MVA orthologs of C11R, F11L, A44L and B8R with a model antigen positioned to use the same translation initiation codon allowed early transgene expression similar to or slightly greater than that achieved by the commonly-used p7.5 or short synthetic promoters. The frequency of antigen-specific CD8+ T cells induced in mice by single shot or adenovirus-prime, rMVA-boost vaccination were similarly equal or marginally enhanced using endogenous promoters at their authentic genomic loci compared to the traditional constructs. The enhancement in immunogenicity observed using the C11R or F11L promoters compared with p7.5 was similar to that obtained with the mH5 promoter compared with p7.5. Furthermore, the growth rates of the viruses were unimpaired and the insertions were genetically stable. Insertion of a transgenic ORF in place of a viral ORF by BAC recombineering can thus provide not only a potent promoter, but also, concomitantly, a suitable insertion site, potentially facilitating development of MVA vaccines expressing multiple recombinant antigens