The domestic and gendered context for retirement

Against a global backdrop of population and workforce ageing, successive UK governments have encouraged people to work longer and delay retirement. Debates focus mainly on factors affecting individuals’ decisions on when and how to retire. We argue that a fuller understanding of retirement can be achieved by recognizing the ways in which individuals’ expectations and behaviours reflect a complicated, dynamic set of interactions between domestic environments and gender roles, often established over a long time period, and more temporally proximate factors. Using a qualitative data set, we explore how the timing, nature and meaning of retirement and retirement planning are played out in specific domestic contexts. We conclude that future research and policies surrounding retirement need to: focus on the household, not the individual; consider retirement as an often messy and disrupted process and not a discrete event; and understand that retirement may mean very different things for women and for men

miR-132/212 knockout mice reveal roles for these miRNAs in regulating cortical synaptic transmission and plasticity

miR-132 and miR-212 are two closely related miRNAs encoded in the same intron of a small non-coding gene, which have been suggested to play roles in both immune and neuronal function. We describe here the generation and initial characterisation of a miR-132/212 double knockout mouse. These mice were viable and fertile with no overt adverse phenotype. Analysis of innate immune responses, including TLR-induced cytokine production and IFNβ induction in response to viral infection of primary fibroblasts did not reveal any phenotype in the knockouts. In contrast, the loss of miR-132 and miR-212, while not overtly affecting neuronal morphology, did affect synaptic function. In both hippocampal and neocortical slices miR-132/212 knockout reduced basal synaptic transmission, without affecting paired-pulse facilitation. Hippocampal long-term potentiation (LTP) induced by tetanic stimulation was not affected by miR-132/212 deletion, whilst theta burst LTP was enhanced. In contrast, neocortical theta burst-induced LTP was inhibited by loss of miR-132/212. Together these results indicate that miR-132 and/or miR-212 play a significant role in synaptic function, possibly by regulating the number of postsynaptic AMPA receptors under basal conditions and during activity-dependent synaptic plasticity

HIV Prevention in Care and Treatment Settings: Baseline Risk Behaviors among HIV Patients in Kenya, Namibia, and Tanzania.

HIV care and treatment settings provide an opportunity to reach people living with HIV/AIDS (PLHIV) with prevention messages and services. Population-based surveys in sub-Saharan Africa have identified HIV risk behaviors among PLHIV, yet data are limited regarding HIV risk behaviors of PLHIV in clinical care. This paper describes the baseline sociodemographic, HIV transmission risk behaviors, and clinical data of a study evaluating an HIV prevention intervention package for HIV care and treatment clinics in Africa. The study was a longitudinal group-randomized trial in 9 intervention clinics and 9 comparison clinics in Kenya, Namibia, and Tanzania (N = 3538). Baseline participants were mostly female, married, had less than a primary education, and were relatively recently diagnosed with HIV. Fifty-two percent of participants had a partner of negative or unknown status, 24% were not using condoms consistently, and 11% reported STI symptoms in the last 6 months. There were differences in demographic and HIV transmission risk variables by country, indicating the need to consider local context in designing studies and using caution when generalizing findings across African countries. Baseline data from this study indicate that participants were often engaging in HIV transmission risk behaviors, which supports the need for prevention with PLHIV (PwP). TRIAL REGISTRATION: ClinicalTrials.gov NCT01256463

nIFTy galaxy cluster simulations – V. Investigation of the cluster infall region

We examine the properties of the galaxies and dark matter haloes residing in the cluster infall region surrounding the simulated $\Lambda$ cold dark matter galaxy cluster studied by Elahi et al. at $z$ = 0. The 1.1 × 10$^{15}$ $h^{−1}$ M$_\odot$ galaxy cluster has been simulated with eight different hydrodynamical codes containing a variety of hydrodynamic solvers and sub-grid schemes. All models completed a dark-matter-only, non-radiative and full-physics run from the same initial conditions. The simulations contain dark matter and gas with mass resolution $m_\text{DM}$ = 9.01 × 10$^8$ $h^{−1}$ M$_\odot$ and $m_\text{gas}$ = 1.9 × 10$^8$ $h^{−1}$ M$_\odot$, respectively. We find that the synthetic cluster is surrounded by clear filamentary structures that contain ~60 per cent of haloes in the infall region with mass ~10$^{12.5}$–10$^{14}$ $h^{−1}$ M$_\odot$, including 2–3 group-sized haloes (>10$^{13}$ $h^{−1}$ M$_\odot$). However, we find that only ~10 per cent of objects in the infall region are sub-haloes residing in haloes, which may suggest that there is not much ongoing pre-processing occurring in the infall region at $z$ = 0. By examining the baryonic content contained within the haloes, we also show that the code-to-code scatter in stellar fraction across all halo masses is typically ~2 orders of magnitude between the two most extreme cases, and this is predominantly due to the differences in sub-grid schemes and calibration procedures that each model uses. Models that do not include active galactic nucleus feedback typically produce too high stellar fractions compared to observations by at least ~1 order of magnitude.The authors would like the acknowledge the Centre for High Performance Computing in Rosebank, Cape Town, for financial support and for hosting the ‘Comparison Cape Town’ workshop in 2016, July. The authors would further like to acknowledge the support of the International Centre for Radio Astronomy Research (ICRAR) node at the University of Western Australia (UWA) in hosting the precursor workshop ‘Perth Simulated Cluster Comparison’ in 2015, March; the financial support of the UWA Research Collaboration Award 2014 and 2015 schemes; the financial support of the ARC Centre of Excellence for All Sky Astrophysics (CAASTRO) CE110001020 and ARC Discovery Projects DP130100117 and DP140100198. We would also like to thank the Instituto de Fisica Teorica (IFT-UAM/CSIC in Madrid) for its support, via the Centro de Excelencia Severo Ochoa Program under Grant No. SEV- 2012-0249, during the three-week workshop ‘nIFTy Cosmology’ in 2014, where the foundation for the whole comparison project was established. JA acknowledges support from a post-graduate award from STFC. PJE is supported by the SSimPL programme and the Sydney Institute for Astronomy (SIfA) and Australian Research Council (ARC) grants DP130100117 and DP140100198. AK is supported by the Ministerio de Econom´ıa y Competitividad (MINECO) in Spain through grant AYA2012-31101 as well as the ConsoliderIngenio 2010 Programme of the Spanish Ministerio de Ciencia e Innovacion (MICINN) under grant MultiDark CSD2009-00064. ´ He also acknowledges support from the ARC grant DP140100198. He further thanks Noonday Underground for surface noise. STK acknowledges support from STFC through grant ST/L000768/1. CP acknowledges the support of the ARC through Future Fellowship FT130100041 and Discovery Project DP140100198. WC and CP acknowledge the support of ARC DP130100117. GY and FS acknowledge support from MINECO (Spain) through the grant AYA 2012-31101. GY thanks also the Red Espanola de Supercomputa- ˜ cion for granting the computing time in the Marenostrum Supercomputer at BSC, where all the MUSIC simulations have been performed. AMB is supported by the DFG Research Unit 1254 ‘Magnetisation of interstellar and intergalactic media’ and by the DFG Cluster of Excellence ‘Universe’. GM acknowledge support from the PRIN-MIUR 2012 Grant ‘The Evolution of Cosmic Baryons’ funded by the Italian Minister of University and Research, by the PRIN-INAF 2012 Grant ‘Multi-scale Simulations of Cosmic Structures’, by the INFN INDARK Grant and by the ‘Consorzio per la Fisica di Trieste’. IGM acknowledges support from an STFC Advanced Fellowship. EP acknowledges support by the ERC grant ‘The Emergence of Structure During the Epoch of Reionization’

The conserved C-terminus of the PcrA/UvrD helicase interacts directly with RNA polymerase

Copyright: © 2013 Gwynn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by a Wellcome Trust project grant to MD (Reference: 077368), an ERC starting grant to MD (Acronym: SM-DNA-REPAIR) and a BBSRC project grant to PM, NS and MD (Reference: BB/I003142/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Static and Moving Frontiers: The Genetic Landscape of Southern African Bantu-Speaking Populations

A consensus on Bantu-speaking populations being genetically similar has emerged in the last few years, but the demographic scenarios associated with their dispersal are still amatter of debate. The frontier model proposed by archeologists postulates different degrees of interaction among incoming agropastoralist and resident foraging groups in the presence of "static" and "moving" frontiers. By combining mitochondrial DNA and Y chromosome data collected from several southern African populations, we show that Bantu-speaking populations from regions characterized by amoving frontier developing after a long-term static frontier have larger hunter-gatherer contributions than groups from areas where a static frontier was not followed by further spatial expansion. Differences in the female and male components suggest that the process of assimilation of the long-term resident groups into agropastoralist societies was gender biased. Our results show that the diffusion of Bantu languages and culture in Southern Africa was a process more complex than previously described and suggest that the admixture dynamics between farmers and foragers played an important role in shaping the current patterns of genetic diversity

Simulating complex social behaviour with the genetic action tree kernel

The concept of genetic action trees combines action trees with genetic algorithms. In this paper, we create a multi-agent simulation on the base of this concept and provide the interested reader with a software package to apply genetic action trees in a multi-agent simulation to simulate complex social behaviour. An example model is introduced to conduct a feasibility study with the described method. We find that our library can be used to simulate the behaviour of agents in a complex setting and observe a convergence to a global optimum in spite of the absence of stable states

Waist Circumference as Compared with Body-Mass Index in Predicting Mortality from Specific Causes

Background Whether waist circumference provides clinically meaningful information not delivered by body-mass index regarding prediction of cause-specific death is uncertain. Methods We prospectively examined waist circumference (WC) and body-mass index (BMI) in relation to cause-specific death in 225,712 U.S. women and men. Cox regression was used to estimate relative risks and 95% confidence intervals (CI). Statistical analyses were conducted using SAS version 9.1. Results During follow-up from 1996 through 2005, we documented 20,977 deaths. Increased WC consistently predicted risk of death due to any cause as well as major causes of death, including deaths from cancer, cardiovascular disease, and non-cancer/non-cardiovascular diseases, independent of BMI, age, sex, race/ethnicity, smoking status, and alcohol intake. When WC and BMI were mutually adjusted in a model, WC was related to 1.37 fold increased risk of death from any cancer and 1.82 fold increase risk of death from cardiovascular disease, comparing the highest versus lowest WC categories. Importantly, WC, but not BMI showed statistically significant positive associations with deaths from lung cancer and chronic respiratory disease. Participants in the highest versus lowest WC category had a relative risk of death from lung cancer of 1.77 (95% CI, 1.41 to 2.23) and of death from chronic respiratory disease of 2.77 (95% CI, 1.95 to 3.95). In contrast, subjects in the highest versus lowest BMI category had a relative risk of death from lung cancer of 0.94 (95% CI, 0.75 to 1.17) and of death from chronic respiratory disease of 1.18 (95% CI, 0.89 to 1.56). Conclusions Increased abdominal fat measured by WC was related to a higher risk of deaths from major specific causes, including deaths from lung cancer and chronic respiratory disease, independent of BMI

Characterizing genomic alterations in cancer by complementary functional associations.

Systematic efforts to sequence the cancer genome have identified large numbers of mutations and copy number alterations in human cancers. However, elucidating the functional consequences of these variants, and their interactions to drive or maintain oncogenic states, remains a challenge in cancer research. We developed REVEALER, a computational method that identifies combinations of mutually exclusive genomic alterations correlated with functional phenotypes, such as the activation or gene dependency of oncogenic pathways or sensitivity to a drug treatment. We used REVEALER to uncover complementary genomic alterations associated with the transcriptional activation of β-catenin and NRF2, MEK-inhibitor sensitivity, and KRAS dependency. REVEALER successfully identified both known and new associations, demonstrating the power of combining functional profiles with extensive characterization of genomic alterations in cancer genomes

Experiencing neutropenia: Quality of life interviews with adult cancer patients

BACKGROUND: Neutropenia is a common toxicity in chemotherapy but detailed information about how neutropenia is associated with changes in patients' quality of life is not readily available. This prospective study interviewed patients with grade 4 neutropenia to provide qualitative information on patients' experience of developing and coping with grade 4 neutropenia during a cycle of chemotherapy. METHODS: A sample of 34 patients who developed grade 4 neutropenia during the first cycle of chemotherapy completed a total of 100 structured clinical interviews. Interviews were transcribed, and 2 raters inductively developed 5 broad categories comprising 80 specific complaint domains nominated by patients. Thirty-five patient-nominated problems were mentioned in 5% or more of the interviews. RESULTS: Fatigue was the most common physical symptom. Interference in daily routine, negative self-evaluation, negative emotion, and social isolation were other common complaints associated with neutropenia. CONCLUSION: Neutropenia is associated with a number of negative experiences among cancer patients undergoing chemotherapy, and these negative experiences have an adverse effect on the patient's quality of life. Oncology nurses can play a key role in helping patients manage adverse effects to maintain their quality of life