Assembly and dynamics of the bacteriophage T4 homologous recombination machinery

Homologous recombination (HR), a process involving the physical exchange of strands between homologous or nearly homologous DNA molecules, is critical for maintaining the genetic diversity and genome stability of species. Bacteriophage T4 is one of the classic systems for studies of homologous recombination. T4 uses HR for high-frequency genetic exchanges, for homology-directed DNA repair (HDR) processes including DNA double-strand break repair, and for the initiation of DNA replication (RDR). T4 recombination proteins are expressed at high levels during T4 infection in E. coli, and share strong sequence, structural, and/or functional conservation with their counterparts in cellular organisms. Biochemical studies of T4 recombination have provided key insights on DNA strand exchange mechanisms, on the structure and function of recombination proteins, and on the coordination of recombination and DNA synthesis activities during RDR and HDR. Recent years have seen the development of detailed biochemical models for the assembly and dynamics of presynaptic filaments in the T4 recombination system, for the atomic structure of T4 UvsX recombinase, and for the roles of DNA helicases in T4 recombination. The goal of this chapter is to review these recent advances and their implications for HR and HDR mechanisms in all organisms

British immigration policy, depoliticisation and Brexit

This paper seeks to problematise the historical significance of the EU for British governing strategy with reference to immigration policy and the concept of depoliticisation. Situating British governing strategy in terms of the crisis-prone nature of capitalist society, this paper argues that British immigration policy has been depoliticised through, initially, the invocation of globalisation and, more recently, the EU. Through this strategy, the British state has been able to repeatedly claim that immigration policy is largely out of its hands, as they have no control over workers wishing to enter Britain looking for work. This paper makes three claims: firstly, immigration policy has been used as a means by both Conservative and Labour governments to manage inflation and labour; secondly, successive governments have sought to depoliticise immigration policy through reference to external forces; thirdly, this strategy of depoliticisation ultimately failed, politicising Britain’s relationship with the EU and creating conditions for Britain’s exit from the EU