19 research outputs found
Tyrosine-protein kinase Yes controls endothelial junctional plasticity and barrier integrity by regulating VE-cadherin phosphorylation and endocytosis
Vascular endothelial (VE)-cadherin in endothelial adherens junctions is an essential component of the vascular barrier, critical for tissue homeostasis and implicated in diseases such as cancer and retinopathies. Inhibitors of Src cytoplasmic tyrosine kinase have been applied to suppress VE-cadherin tyrosine phosphorylation and prevent excessive leakage, edema and high interstitial pressure. Here we show that the Src-related Yes tyrosine kinase, rather than Src, is localized at endothelial cell (EC) junctions where it becomes activated in a flow-dependent manner. EC-specific Yes1 deletion suppresses VE-cadherin phosphorylation and arrests VE-cadherin at EC junctions. This is accompanied by loss of EC collective migration and exaggerated agonist-induced macromolecular leakage. Overexpression of Yes1 causes ectopic VE-cadherin phosphorylation, while vascular leakage is unaffected. In contrast, in EC-specific Src deficiency, VE-cadherin internalization is maintained and leakage is suppressed. In conclusion, Yes-mediated phosphorylation regulates constitutive VE-cadherin turnover, thereby maintaining endothelial junction plasticity and vascular integrity
Physics with the KLOE-2 experiment at the upgraded DANE
Investigation at a --factory can shed light on several debated issues
in particle physics. We discuss: i) recent theoretical development and
experimental progress in kaon physics relevant for the Standard Model tests in
the flavor sector, ii) the sensitivity we can reach in probing CPT and Quantum
Mechanics from time evolution of entangled kaon states, iii) the interest for
improving on the present measurements of non-leptonic and radiative decays of
kaons and eta/eta mesons, iv) the contribution to understand the
nature of light scalar mesons, and v) the opportunity to search for narrow
di-lepton resonances suggested by recent models proposing a hidden dark-matter
sector. We also report on the physics in the continuum with the
measurements of (multi)hadronic cross sections and the study of gamma gamma
processes.Comment: 60 pages, 41 figures; added affiliation for one of the authors; added
reference to section
Scalable parallel preconditioners for an open source cardiac electrophysiology simulation package
Shock−induced arrhythmogenesis in the human heart: a computational modelling study
Electrical defibrillation by application of a strong shock to the heart is the only effective treatment against lethal cardiac arrhythmias such as ventricular fibrillation. A large body of experimental and computational research has been devoted to understanding shock-induced effects on the heart in an attempt to improve defibrillation efficacy. However, most of the research has been performed in small animal hearts, and in particular rabbits. The difference in size between rabbits and humans might limit the extrapolation of the results to the clinical setting. In this paper, we present, for the first time, computer simulations of shock-induced effects on a human ventricular model with realistic ion channel dynamics and fibre architecture. Bidomain simulations using the human ventricular model were performed using the Chaste open source simulation package. The parallel performance of the software package was highly improved in order to meet the computational requirements of these kind of studies
Sähkötilojen kuntokartoitus
Opinnäytetyö käsittelee Tampereen Energiantuotanto Oy:n Pirkanmaan alueella sijaitsevien kiinteiden lämpökeskusten sähkötilojen ja muun valitun sähkölaitteiston kuntokartoitusta ja parannusehdotusten kustannusarviota. Työn tavoitteena oli kerätä tiivis tietopaketti lämpökeskusten sähkölaitteiston kunnosta ja tehdä parannusehdotuksista kustannusarviot päätöksenteon tueksi.
Tavoitteisiin pyrittiin tutustumalla voimassa oleviin standardeihin, turvallisuusmääräyksiin ja tarkastuskäytäntöihin sekä laatimalla tarkastuspöytäkirja. Tarkastuspöytäkirjojen pohjalta tehtiin yhteenvedot ja sähkölaitteiston parannusehdotukset. Parannusehdotusten kustannusarviot koottiin käyttämällä tuotteiden listahintoja ja tilaajan varastohenkilökunnalta ja asiantuntijoilta saatuja tietoja.
Tarkastettu sähkölaitteisto oli laaja ja asennettu viidellä eri vuosikymmenellä. Laitteistossa oli hyvä-, tyydyttävä- ja välttäväkuntoisia laitteita. Yhtään huonokuntoista laitteistoa, jolla olisi välitön uusimis- tai korjaustarve, ei löytynyt. Kustannusarviot olivat n. 100–50 000 €. Suurin osa kustannusarvioiden summista oli minimaalisia, muutamia satoja euroa. Kustannusarvioiden suurimmat summat olivat Julkujärven ja Hervannan lämpökeskuksien parannusehdotuksilla.
Johtopäätöksenä todettiin, että sähkölaitteisto oli yleisesti hyväkuntoinen. Julkujärven ja Hervannan lämpökeskusten parannusehdotusten pohjalta olisi hyvä tehdä lisätutkimuksia, joiden avulla selvitettäisiin uudistus- tai korjaustarve yksityiskohtaisemmin. Lisätutkimukset auttaisivat laitteiston lopullisen kunnostus- ja parannusohjelman laatimisessa.This bachelor’s thesis is about condition audit, proposals for improvements and estimates of their prices in Tampereen Energiantuotanto Ltd.’s fixed heating plants in Pirkanmaa area. The goals were to gather a compact information package about the condition of electrical installations and to estimate the costs of proposals for improvements to support decision making.
In order to meet these goals valid standards, safety regulations and running practice were studied and audit minutes were compiled. Based on the minutes summaries and proposals for improvements were made. When estimating the costs of the improvement proposals, retail prices and the warehouse staff’s as well as expert’s knowledge were taken into account.
The number of inspected electrical installations was high. The installations had been made in five decades. Electrical installations in good, satisfactory and tolerable conditions were found. There were no installations which would have been in bad condition and would have needed immediate repair. The cost estimates were between 100–50 000 €. The most of the cost estimates were small sums, a few hundred euros. In Julkujärvi and Hervanta heating plants cost estimates were the largest ones.
As a conclusion the condition of electrical installations was good in general. In Julkujärvi and Hervanta heating plants further detections should be made in more detailed way in order to make renovation and improvement plans for the installation