Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization

In the present study, we propose a green route to prepare poly(3-hydroxybutyrate) [(P(3HB)] grafted ethyl cellulose (EC) based green composites with novel characteristics through laccase-assisted grafting. P(3HB) was used as a side chain whereas, EC as a backbone material under an ambient processing conditions. A novel laccase obtained from Aspergillus niger through its heterologous expression in Saccharomyces cerevisiae was used as a green catalyst for grafting purposes without the use of additional initiator and/or cross-linking agents. Subsequently, the resulting P(3HB)-g-EC composites were characterized using a range of analytical and imagining techniques. Fourier transform infrared spectroscopy (FT-IR) spectra showed an increase in the hydrogen-bonding type interactions between the side chains of P(3HB) and backbone material of EC. Evidently, X-ray diffraction (XRD) analysis revealed a decrease in the crystallinity of the P(3HB)-g-EC composites as compared to the pristine individual polymers. A homogeneous P(3HB) distribution was also achieved in case of the graft composite prepared in the presence of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a mediator along with laccase as compared to the composite prepared using pure laccase alone. A substantial improvement in the thermal and mechanical characteristics was observed for grafted composites up to the different extent as compared to the pristine counterparts. The hydrophobic/hydrophilic properties of the grafted composites were better than those of the pristine counterparts

PLANT GROWTH POTENTIAL OF SALT TOLERANT ENDOPHYTE Pseudomonas Sihuiensis ISOLATED FROM CHICKPEA

Bacterial endosymbionts are well characterized for plant growth promotion. In this study, the root, nodules, and stem of the Cicer arietinum crop planted in a semi-arid zone were used as a source to isolate potential plant growth bacteria. The ability to grow under salt stress was determined, and the potential isolate was screened for plant growth promotion traits. The selected isolate was identified by the 16S rDNA method. Pot trials were conducted to know the ability of the isolate to promote plant growth in-vivo. Among various isolates obtained, a bacterial isolate obtained from root showed the ability to grow in the presence of 10 % Sodium fluoride (NaF). The isolate produced Indole Acetic acid in an amount of 72 mg per liter in production medium. The bacteria solubilized phosphate and produce exopolysaccharide (2.12 g per liter). The isolate was identified as Pseudomonas sihuiensis. The result of pot trials reveals that the endophyte promotes plant growth under stress conditions and may be used as a bio-fertilizer.   &nbsp

Impacts of different tillage practices on soil water infiltration for sustainable agriculture

Over the years, cultivation using sustainable tillage practices has gained significant importance, but the impact of tillage on soil water infiltration is still a concern for landowners due to the possible effects on crop yield. This study investigates the impact of different tillage managements on the infiltration rate of sandy clay loam soil under a semiarid environment. Field experiments were conducted in Chott Mariem Sousse, Tunisia. The tillage practices consisted of three treatments, including a tine cultivator (TC, 16 cm), moldboard plows (MP, 36 cm) and no-tillage (NT). Three infiltration models, Kostiakov, Philip and Horton, were applied to adjust the observed data and evaluate the infiltration characteristics of the studied soils. Comparison criteria, including the coefficient of determination (R2 ), along with the root mean square error (RMSE) and mean absolute error (MAE), were used to investigate the best-fit model. The results showed that moldboard plowing enhanced soil infiltration capacity relative to tine cultivation and no-tillage treatments. The mean saturated hydraulic conductivity was highest under MP, while it was lowest in NT, with 33.4% and 34.1% reduction compared to TC and MP, respectively. Based on the obtained results, Philip’s model showed better results with observed infiltration due to a higher R2 (0.981, 0.973 and 0.967), lower RMSE (3.36, 9.04 and 9.21) and lower MAE (1.46, 3.53 and 3.72) recorded, respectively, for NT, MP and TC. Horton’s model had a low regression coefficient between observed and predicted values. It was suggested that the Philip two-term model can adequately describe the infiltration process in the study area

Socio‐economic and environmental impacts of biomass valorisation:A strategic drive for sustainable bioeconomy

In the late twentieth century, the only cost-effective opportunity for waste removal cost at least several thousand dollars, but nowadays, a lot of improvement has occurred. The biomass and waste generation problems attracted concerned authorities to identify and provide environmentally friendly sustainable solutions that possess environmental and economic benefits. The present study emphasises the valorisation of biomass and waste produced by domestic and industrial sectors. Therefore, substantial research is ongoing to replace the traditional treatment methods that potentially acquire less detrimental effects. Synthetic biology can be a unique platform that invites all the relevant characters for designing and assembling an efficient program that could be useful to handle the increasing threat for human beings. In the future, these engineered methods will not only revolutionise our lives but practically lead us to get cheaper biofuels, producing bioenergy, pharmaceutics, and various biochemicals. The bioaugmentation approach concomitant with microbial fuel cells (MFC) is an example that is used to produce electricity from municipal waste, which is directly associated with the loading of waste. Beyond the traditional opportunities, herein, we have spotlighted the new advances in pertinent technology closely related to production and reduction approaches. Various integrated modern techniques and aspects related to the industrial sector are also discussed with suitable examples, including green energy and other industrially relevant products. However, many problems persist in present-day technology that requires essential efforts to handle thoroughly because significant valorisation of biomass and waste involves integrated methods for timely detection, classification, and separation. We reviewed and proposed the anticipated dispensation methods to overcome the growing stream of biomass and waste at a distinct and organisational scale

Higher meson resonances in ρ→π0π0γ\rho \to \pi^0 \pi^0 \gamma and ω→π0π0γ\omega \to \pi^0 \pi^0 \gamma

The role of higher meson resonances with spin 1 and 2 is investigated quantitatively in the decay processes of $\rho \to \pi^0\pi^0 \gamma$ and $\omega \to \pi^0 \pi^0 \gamma$. Among the higher resonances, we find that the $f_2(1270)$ tensor meson can give a nontrivial contribution especially to the $\omega \to \pi^0 \pi^0 \gamma$ decay process. When the $f_2$ contribution is combined with the processes involving the vector and scalar meson intermediate states, a good agreement with the recent measurements is achieved for both decays. The effect of the $f_2(1270)$ is found to be sizable at the intermediate photon energies and may be verified by precise measurements of the recoil photon spectrum of the $\omega \to \pi^0 \pi^0 \gamma$ decay. The dependence of the decay widths on various models for the $\rho$-$\omega$ mixing in the literature is also investigated.Comment: 16 pages, REVTeX, 6 figures, revised version, to appear in Phys. Rev.

Two-dimensional infrared correlation spectroscopy, conductor-like screening model for real solvents, and density functional theory study on the adsorption mechanism of polyvinylpolypyrrolidone for effective phenol removal in an aqueous medium

The discharge of industrial effluents, such as phenol, into aquatic and soil environments is a global problem due to its serious negative impacts on human health and aquatic ecosystems. In this study, the ability of polyvinylpolypyrrolidone (PVPP) to remove phenol from an aqueous medium was investigated. The results showed that a significant proportion of phenol (up to 74.91%) was removed using PVPP at pH 6.5. Isotherm adsorption experiments of phenol on PVPP indicated that the best-fit adsorption was obtained using Langmuir models. The response peaks of the hydroxyl groups of phenol (OH) and the carboxyl groups (i.e., C=O) of PVPP were altered, indicating the formation of a hydrogen bond between the PVPP and phenol during phenol removal, as characterized using 1D and 2D IR spectroscopy. The resulting complexes were successfully characterized based on their thermodynamic properties, Mulliken charge, and electronic transition using the DFT approach. To clarify the types of interactions taking place in the complex systems, quantum theory of atoms in molecules (QTAIM) analysis, reduced density gradient noncovalent interaction (RDG-NCI) approach, and conductor-like screening model for real solvents (COSMO-RS) approach were also successfully calculated. The results showed that the interactions that occurred in the process of removing phenol by PVPP were through hydrogen bonding (based on RDG-NCI and COSMO-RS), which was identified as an intermediate type (∇2ρ(r) > 0 and H < 0, QTAIM). To gain a deeper understanding of how these interactions occurred, further characterization was performed based on adsorption mechanisms using molecular electrostatic potential, global reactivity, and local reactivity descriptors. The results showed that during hydrogen bond formation, PVPP acts as a nucleophile, whereas phenol acts as an electrophile and the O9 atom (i.e., donor electron) reacts with the H22 atom (i.e., acceptor electron)

The Dipion Mass Spectrum In e+e- Annihilation and tau Decay: A Dynamical (rho0, omega, phi) Mixing Approach

We readdress the problem of finding a simultaneous description of the pion form factor data in e+e- annihilations and in tau decays. For this purpose, we work in the framework of the Hidden Local Symmetry (HLS) Lagrangian and modify the vector meson mass term by including the pion and kaon loop contributions. This leads us to define the physical rho, omega and phi fields as linear combinations of their ideal partners, with coefficients being meromorphic functions of s, the square of the 4--momentum flowing into the vector meson lines. This allows us to define a dynamical, i.e. s-dependent, vector meson mixing scheme. The model is overconstrained by extending the framework in order to include the description of all meson radiative (V P gamma and P gamma gamma couplings) and leptonic (Ve+e- couplings) decays and also the isospin breaking (omega/ phi --> pi+ pi-) decay modes. The model provides a simultaneous, consistent and good description of the e+e- and tau dipion spectra. The expression for pion form factor in the latter case is derived from those in the former case by switching off the isospin breaking effects specific to e+e- and switching on those for tau decays. Besides, the model also provides a good account of all decay modes of the form V P gamma, Pgamma gamma as well as the isospin breaking decay modes. It leads us to propose new reference values for the rho^0 --> e+ e- and omega --> pi+ pi- partial widths which are part of our description of the pion form factor. Other topics (phi --> K anti K, the rho meson mass and width parameters) are briefly discussed. Therefore, we confirm the 3.3 sigma discrepancy between the theoretical estimate of a_mu based on e+e- and its direct BNL measurement.Comment: 71 pages, 8 figures. Accepted by EPJ C. Version 3: correct minor typos, minor changes spread out into the text. Extension of Sections 12.2 and 12.3.5 and introduction of the new Appendix

Comparative study on the valorization of Sargassum from the Mexican Caribbean coast and Gulf of California as an ingredient on healthy diets for shrimp farming

Funding: This work received the financial support of the project GCRFNGR4/1388 "Algae bloom: waste resource for aquaculture and bioenergy industry in Mexico".Sargassum biomass is a potential ingredient for aquaculture formulations due to its high nutritional value and availability, as it contains a variety of essential nutrients including proteins, carbohydrates, lipids, vitamins, antioxidants, fiber, and minerals, which are essential for aquatic growing, development and health parameters including digestibility and immune response against diseases. Therefore, in this work, five experimental diets were formulated considering 2.5 % and 5 % levels of inclusion of Sargassum spp. from the Mexican Caribbean coasts (SC), and from the Gulf of California (SG), and one reference diet as a control. The characterization of the raw material and the proximal analysis of the five diets were evaluated according to standard reference methods. A 35-day feeding trial was conducted using juvenile Litopenaeus vannamei showed a shrimp survival rate of 97 ± 6 % (p > 0.05) on SC. Excellent weight gain (WG) performance of SC and SG diets at 2.5 % of inclusion resulted in >10 % WG compared to the control. The specific activity of amylase, lipase, trypsin, and chymotrypsin showed a significant increase by including Sargassum in diets, compared with the reference diet. Incorporating Sargassum at 2.5 % in feed formulations for shrimps showed a great opportunity to use it as an ingredient and improved production yields. The digestion and absorption parameters were represented by the enzymatic activity in which the diets based on Sargassum spp. from the Mexican Caribbean coast (SC) have resulted in the best performance.Publisher PDFPeer reviewe

Perceptions on the accessibility of Islamic banking in the UK—Challenges, opportunities and divergence in opinion

This study examines the views of UK-based Muslims, Islamic Scholars and Islamic banking employees on the current state of the latter industry, both in practical terms and as regards engagement with the nation’s large, but often marginalised Islamic community. The British Government has recently championed the Islamic banking sector and committed to supporting it as a means of addressing financial services needs and consolidating London’s position as the global centre for Islamic investment. The analysis adds to the substantive literature in two principal ways: (i) by contextualising the evidence via the notions of empowerment, engagement and social justice that underpin both the state’s attempts to foster growth and the central tenets of Islam; and (ii) by placing comparison of the opinions of key groups at the heart of the investigation. The findings reveal that while progress has been made, UK-based Muslims see several substantive impediments to access, including the complex terminology of Islamic banking products, the lack of internet banking facilities and branch networks as well as a generalised lack of interest in marketing on the part of the institutions. Whilst some coincidence of perception is evident, the views of bankers are shown to be out of line with those of the other parties in a number of key areas. For example, bankers appear to see less potential in the role of the internet as a medium for spreading awareness than do either potential customers or religious scholars. The paper therefore concludes with a call for multi-party Ijtihad and Qiyas (deductive analogy) that will encourage industrial outreach and, in so doing, support long-term growth

Environmental persistence, detection, and mitigation of endocrine disrupting contaminants in wastewater treatment plants – a review with a focus on tertiary treatment technologies

Endocrine disrupting chemicals are a group of contaminants that have severe effects on humans and animals when exposed, like cancer and alterations to the nervous and reproductive systems. The increasing concentrations of several endocrine disrupting chemicals in the environment are strongly related to anthropogenic activities, and as the population grows this problem becomes more relevant. Thus, wastewater is one of the main sources of endocrine disrupting chemicals, and the technologies employed during primary and secondary treatment in wastewater treatment plants cannot remove these contaminants. Due to this, researchers have tried to develop more efficient technologies for tertiary treatment of wastewater and reduce the concentration of endocrine disrupting chemicals discharged into the environment. Some of the most promising technologies include adsorption, ultrafiltration, advanced oxidation processes and biodegradation. The use of nanomaterials as adsorbents, catalysts, membranes and supports has played a key role in enhancing the efficiency of these technologies. The results showed that these technologies have great potential on the lab-scale, and even some of them have already been employed at some wastewater treatment plants. However, there are still some challenges to achieving a global implementation of these technologies, related to reducing the costs of materials and enhancing their current performance. The use of biomass/waste derived carbon materials and implementing hybrid technologies are accessible approaches for their implementation in tertiary treatment.This work is part of the project entitled “Contaminantes emergentes y prioritarios en las aguas reutilizadas en agricultura: riesgos y efectos en suelos, producción agrícola y entorno ambiental” funded by CSIC-Tecnológico de Monterrey under the i-Link + program (LINKB20030). The author “Jesús Alfredo Rodríguez-Hernández” acknowledges Consejo Nacional de Ciencia y Tecnología (CONACyT) for awarding a scholarship for a PhD in nanotechnology (CVU: 924193). CONACyT is thankfully acknowledged for partially supporting this work under the Sistema Nacional de Investigadores (SNI) program awarded to Rafael G. Araújo (CVU: 714118), Juan Eduardo Sosa-Hernández (CVU: 375202), Elda M. Melchor-Martínez (CVU: 230784), Manuel Martinez-Ruiz (CVU: 418151), Hafiz M. N. Iqbal (CVU: 735340) and Roberto Parra-Saldívar (CVU: 35753). The authors are also thankful to “Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo” in the Latin American development network “Lacasas Inmovilizadas para la Degradación de Compuestos Aromáticos en Aguas Residuales” (LIDA, project 318RT0552). All listed authors are also grateful to their representative universities/institutes for providing literature facilities and the Biorender online program for the elaboration of the graphical abstract and Fig. 1–5.Peer reviewe