Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers

The X-ray crystal structure of d(TGCGCA)(2) has been determined at 120 K to a resolution of 1.3 Angstrom. Hexamer duplexes, in the Z-DNA conformation, pack in an arrangement similar to the 'pure spermine form' [Egli et al. (1991). Biochemistry, 30, 11388-11402] but with significantly different cell dimensions. The phosphate backbone exists in two equally populated discrete conformations at one nucleotide step, around phosphate 11. The structure contains two ordered cobalt hexammine molecules which have roles in stabilization of both the Z-DNA conformation of the duplex and in crystal packing. A comparison of d(TGCGCA)(2) with other Z-DNA hexamer structures available in the Nucleic Acid Database illustrates the elusive nature of crystal packing. A review of the interactions with the metal cations Na+, Mg2+ and Co3+ reveals a relatively small proportion of phosphate binding and that close contacts between metal ions are common. A prediction of the water structure is compared with the observed pattern in the reported structure

Parenting stress, maternal depression and child mental health in a Melbourne cohort before and during the COVID‐19 pandemic

Aim This paper aims to examine the maternal and child mental health and parenting outcomes in the context of COVID-19 pandemic conditions using a sample from Melbourne, Australia – a city exposed to one of the longest lockdowns world-wide in response to the pandemic. Methods This study utilises observational data from a prospective, pregnancy cohort, Mercy Pregnancy Emotional Wellbeing Study and includes 468 women and their children followed up in Melbourne to 3–4 years postpartum pre-COVID pandemic and compared to those followed up during the COVID-19 pandemic. Results When compared to mothers followed up at 3–4 years postpartum pre-pandemic, those followed up during the COVID-19 pandemic showed higher depressive symptoms with a steep incline in their symptom trajectory (EMMdifference = 1.72, Bonferroni-corrected P < 0.01, d = 0.35) and had a three times higher risk of scoring 13 or above on the EPDS (aRR = 3.22, Bonferroni-corrected P < 0.01). Although this increase was not associated with the variation in the duration of exposure to pandemic conditions, the steep increase in depressive symptoms was more pronounced in those with pre-existing depressive disorders. There was no difference in parenting stress or adjusted childhood mental health symptoms or disorder. Conclusions Our findings highlight the vulnerability of those with pre-existing clinical mental health disorders and the need for adequate clinical care for this vulnerable group. Equally, our study indicates the possibility that parenting and early childhood mental health outcomes, at least in the short term, may be resilient

Are Antiprotons Forever?

Up to one million antiprotons from a single LEAR spill have been captured in a large Penning trap. Surprisingly, when the antiprotons are cooled to energies significantly below 1 eV, the annihilation rate falls below background. Thus, very long storage times for antiprotons have been demonstrated in the trap, even at the compromised vacuum conditions imposed by the experimental set up. The significance for future ultra-low energy experiments, including portable antiproton traps, is discussed.Comment: 12 pages, latex; 4 figures, uufiled. Slightly expanded discussion of expected energy dependence of annihilation cross section and rate, and of estimates of trap pressure, plus minor text improvement

Wear and damage transitions of wheel and rail materials under various contact conditions

This study discusses a Tγ/A method of plotting wear data from a twin-disc machine for identifying the wear and damage transitions of wheel and rail materials. As found in previous work, three wear regimes (mild wear, severe wear and catastrophic wear) of U71Mn rail material were identified in dry rolling-sliding contact tests. It was determined that the damage mechanism transforms in the different wear regimes. Here earlier studies were extended to establish wear behavior for the presence of a number of third body materials (oil, water, friction enhancers) and a rail cladding process designed to make wheels and rails more durable. This has provided much needed data for Multi-Body Dynamics (MBD) simulations, and will allow better predictions of profile evolution of wheel and rail over a wider range of conditions

Constraining Warm Inflation with the Cosmic Microwave Background

We discuss the spectrum of scalar density perturbations from warm inflation when the friction coefficient $\Gamma$ in the inflaton equation is dependent on the inflaton field. The spectral index of scalar fluctuations depends on a new slow-roll parameter constructed from $\Gamma$. A numerical integration of the perturbation equations is performed for a model of warm inflation and gives a good fit to the WMAP data for reasonable values of the model's parameters.Comment: 6 pages, 2 figures, RevTeX

Face Detection on Embedded Systems

Over recent years automated face detection and recognition (FDR) have gained significant attention from the commercial and research sectors. This paper presents an embedded face detection solution aimed at addressing the real-time image processing requirements within a wide range of applications. As face detection is a computationally intensive task, an embedded solution would give rise to opportunities for discrete economical devices that could be applied and integrated into a vast majority of applications. This work focuses on the use of FPGAs as the embedded prototyping technology where the thread of execution is carried out on an embedded soft-core processor. Custom instructions have been utilized as a means of applying software/hardware partitioning through which the computational bottlenecks are moved to hardware. A speedup by a factor of 110 was achieved from employing custom instructions and software optimizations

Asymmetric Dark Matter and Dark Radiation

Asymmetric Dark Matter (ADM) models invoke a particle-antiparticle asymmetry, similar to the one observed in the Baryon sector, to account for the Dark Matter (DM) abundance. Both asymmetries are usually generated by the same mechanism and generally related, thus predicting DM masses around 5 GeV in order to obtain the correct density. The main challenge for successful models is to ensure efficient annihilation of the thermally produced symmetric component of such a light DM candidate without violating constraints from collider or direct searches. A common way to overcome this involves a light mediator, into which DM can efficiently annihilate and which subsequently decays into Standard Model particles. Here we explore the scenario where the light mediator decays instead into lighter degrees of freedom in the dark sector that act as radiation in the early Universe. While this assumption makes indirect DM searches challenging, it leads to signals of extra radiation at BBN and CMB. Under certain conditions, precise measurements of the number of relativistic species, such as those expected from the Planck satellite, can provide information on the structure of the dark sector. We also discuss the constraints of the interactions between DM and Dark Radiation from their imprint in the matter power spectrum.Comment: 22 pages, 5 figures, to be published in JCAP, minor changes to match version to be publishe

Isothermal and Cyclic Aging of 310S Austenitic Stainless Steel

Unusual damage and high creep strain rates have been observed on components made of 310S stainless steel subjected to thermal cycles between room temperature and 1143 K (870 °C). Microstructural characterization of such components after service evidenced high contents in sigma phase which formed first from δ-ferrite and then from γ-austenite. To get some insight into this microstructural evolution, isothermal and cyclic aging of 310S stainless steel has been studied experimentally and discussed on the basis of numerical simulations. The higher contents of sigma phase observed after cyclic agings than after isothermal treatments are clearly associated with nucleation triggered by thermal cycling