Course of infection with Lymphocystis disease virus in gilthead seabream (Sparus aurata)

Lymphocystis disease virus (LCDV) is the etiological agent of lymphocystis disease (LCD), a pathology that affects a wide variety of fish species. Data about LCDV pathogenesis are very short, and mainly limited to histopathological studies of skin lesions. Recent studies on viral genome detection (both by PCR or DNA-DNA in situ hybridization) suggest that LCDV establish a systemic and persistent infection in gilthead seabream (Sparus aurata), but further studies are necessary to prove if this infection is productive or not. In the present study viral quantification and viral mRNA detection (by qPCR and RT-qPCR) have been used to investigate LCDV multiplication in different organs of juvenile gilthead seabream. In addition, a histopathological study was carried out. Animals were collected from two commercial farms in Southwestern Spain. In one farm, where no LCD outbreaks have been recorded, apparently healthy fish were collected, whereas in the other farm, diseased and recovered (two months after LCD symptoms disappearance) fish were sampled. All the animals were LCDV-infected, and viral gene expression was detected in every organ analysed (caudal fin, intestine, liver, spleen, kidney and brain). In asymptomatic animals, both apparently healthy and recovered, a low-titre infection was observed, with the highest viral copy numbers detected in brain and kidney. In diseased fish, viral loads were significantly higher than in subclinical infected animals, being maximal in caudal fin, where lymphocysts were present in the dermis. Different histological alterations were observed in the internal organs from diseased fish analysed, although no hypertrophied cells were detected in any of them. In recovered fish, most of the organs examined presented similar histological features to those in healthy animals. Thus, pathological changes were only detected in the intestine and liver, although they were less severe than those observed in diseased fish. The results presented showed that LCDV establishes a systemic infection in juvenile gilthead seabream, which can be subclinical. In addition, although the disease is self-limiting, the virus is not removed after disease recovery, but produces a persistent infection.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Evidence of a massive planet candidate orbiting the young active K5V star BD+20 1790

Original article can be found at: http://www.aanda.org/ Copyright The European Southern Observatory (ESO). DOI: 10.1051/0004-6361/200811000Context. BD+20 1790 is a young active, metal-rich, late-type K5Ve star. We have undertaken a study of stellar activity and kinematics for this star over the past few years. Previous results show a high level of stellar activity, with the presence of prominence-like structures, spots on the surface, and strong flare events, despite the moderate rotational velocity of the star. In addition, radial velocity variations with a semi-amplitude of up to 1 km s-1 were detected. Aims. We investigate the nature of these radial velocity variations, in order to determine whether they are due to stellar activity or the reflex motion of the star induced by a companion. Methods. We have analysed high-resolution echelle spectra by measuring stellar activity indicators and computing radial velocity (RV) and bisector velocity spans. Two-band photometry was also obtained to produce the light curve and determine the photometric period. Results. Based upon the analysis of the bisector velocity span, as well as spectroscopic indices of chromospheric indicators, Ca ii H & K, Hα, and taking the photometric analysis into account, we report that the best explanation for the RV variation is the presence of a substellar companion. The Keplerian fit of the RV data yields a solution for a close-in massive planet with an orbital period of 7.78 days. The presence of the close-in massive planet could also be an interpretation for the high level of stellar activity detected. Since the RV data are not part of a planet search programme, we can consider our results as a serendipitous evidence of a planetary companion. To date, this is the youngest main sequence star for which a planetary candidate has been reported.Peer reviewe

Formal Study of a Novel Network Role-based Routing Intelligent Algorithm

AbstractNORIA (Network rOle-based Routing Intelligent Algorithm) is a novel routing algorithm for Wireless Sensor Networks (WSNs) which combines various effective techniques in order to reduce energy consumption and improve data routes. This paper presents a formal and rigorous study of NORIA. Prioritised-Timed Coloured Petri Nets (PTCPNs) have been used to describe complete and unambiguous specifications of system behaviour, whereas CPNTools is used to evaluate the correctness of the protocol using state space exploration

Los prebióticos tipo inulina en alimentación aviar. I: características y efectos a nivel intestinal.

Tras la prohibición por la Unión Europea en 2006 del uso de los antibióticos promotores del crecimiento (APC) en alimentación animal, los investigadores tuvieron que proponer alternativas a los productores de broilers para sustituir estos APC. Los prebióticos se definieron en 1995 como ingredientes alimentarios no digestibles que afectan de manera favorable al hospedador mediante la estimulación del crecimiento de bacterias intestinales beneficiosas. Dentro de los diferentes prebióticos estudiados, la inulina ha destacado debido a los resultados alentadores obtenidos sobre diferentes parámetros. En este trabajo se revisan, en primer lugar, los efectos de los prebióticos del tipo de la inulina sobre el intestino de los broilers, tanto a nivel macroscópico (longitud intestinal) como microscópico (tamaño y densidad de vellosidades y microvellosidades), así como sobre la microbiota intestinal. En segundo lugar, se hace una puesta al día de la información publicada relativa a la influencia de la inclusión de inulina en la ración sobre la digestibilidad de los diferentes nutrientes.Following the EU ban in 2006 on the use of antibiotic growth promoters (AGP) in animal feeding there was a need to find some alternatives to replace AGP in broiler production. Prebiotics were defined in 1995 as non-digestible food ingredients that favourably affect the host by stimulating the growth of beneficial intestinal bacteria. Among the different studied prebiotics, the use of inulin has been highlighted in several studies due to the encouraging results obtained on different parameters. This paper reviews, first, the effects of inulin-type prebiotics on intestinal morphology, both on macroscopic (intestinal length) and microscopic (size and density of villi and microvilli) structures and on the intestinal microbiota in the gut of broiler chickens. Finally, there is an update of the published information concerning the influence of the dietary inclusion of inulin on nutrient digestibility

Purple dwarfs : New L subdwarfs from UKIDSS and SDSS

This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.The first L subdwarf was a discovered only ten years ago. Less than ten L subdwarfs been published in the literature to date. Metal-poor ultracool atmospheres has not been well understood. Halo mass function cross substellar limit has not been measured. We used UKIDSS and SDSS to search for L subdwarfs. We have confirmed some new L subdwarfs and are following up more candidates with ground based large telescopes. We discussed spectral features of L subdwarfs and halo brown dwarfs

Benchmark low-mass objects in Moving Groups

This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.In order to compile a sample of ultracool dwarfs that will serve as benchmarks for testing theoretical formation and evolutionary models, we selected low-mass cool (>M7) objects that are potentially members of five known young Moving Groups in the solar neighbourhood. We have studied the kinematics of the sample, finding that 49 targets belong to the young disk area, from which 36 are kinematic member of one of the five moving groups under study. Some of the identified low-mass members have been spectroscopically characterised (T-eff, log g) and confirmed as young members through a detailed study of age indicators

Prediction of Zamorano cheese quality by near-infrared spectroscopy assessing false non-compliance and false compliance at minimum permitted limits stated by designation of origin regulations

Near-infrared transmittance (NIT) spectroscopy was used to predict the percentage in weight of the fat, dry matter, protein and fat/dry matter contents in Zamorano cheeses, protected with a Designation of Origin by the European Union. A total of 42 cheeses submitted to official control were analysed by reference methods. Samples were scanned (850–1050 nm) and predictive equations were developed using Partial Least Squares regression with a cross-validation step. Eight pretreatments independent from the remaining calibration samples were first considered. The most adequate one was that performing the second derivative (using a Savitzky–Golay method with a nine-point window and a second-order polynomial) followed by the standard normal variate transformation. Percentages of the root mean square error in cross-validation, the coefficient of determination and the mean of the absolute value of relative errors found were, respectively, for fat (0.62; 96.16; 1.05), dry matter (0.76; 96.03; 0.83), protein (0.41; 97.82; 0.81) and the fat/dry matter ratio (0.61; 92.51; 0.66). At a 99% confidence level, the trueness of the NIT+PLS methods for fat, dry matter and protein was verified. The official regulation for Zamorano cheese demands minimum permitted limits on the percentages in weight for protein (25%), dry matter (55%) and the ratio of fat to dry matter (45%). The adaptation of both the decision limit and the detection capability to the case of a minimum permitted limit (CDα and CDβ, respectively) when a Partial Least Squares calibration is used has been applied for the first time for a food product protected by a Designation of Origin. The values of CDα with a probability of false non-compliance equal to 0.05 and of CDβ when, in addition, the probability of false compliance was equal to or less than 0.05, both provided by the corresponding NIT+PLS-based method, were, respectively, for protein (24.78%; 24.57%), dry matter (54.14%; 53.28%) and the fat/dry matter ratio (44.39%; 43.78%).authorsthankthefinancialsupportprovidedbyMinisterio de CienciaeInnovacio´n (CTQ2011-26022)andJuntadeCastillay Leo´n (BU108A11-2

Prenatal experience with language shapes the brain

Human infants acquire language with notable ease compared to adults, but the neural basis of their remarkable brain plasticity for language remains little understood. Applying a scaling analysis of neural oscillations to address this question, we show that newborns' electrophysiological activity exhibits increased long-range temporal correlations after stimulation with speech, particularly in the prenatally heard language, indicating the early emergence of brain specialization for the native language.</p