Shift of microbial communities and reduced enzymatic activity in soil under plastic mulching system in strawberry cultivation

The use of plastic mulching (PM) in agriculture has strongly increased in the last years. Improved water saving and higher soil temperature are some advantages of this management. Yet, an intensive use of PM has been recently linked to negative effects on soil quality. The aim of this study is thus to assess the effects of long-term plastic mulching (PM) on soil microbial indicators. PM was compared with the use of wheat straw mulching (SM), an also widely used mulch material. Samples were collected at two depths (0-5 and 5-10 cm) from strawberry fields, after 4-year management. Cultivation in PM and SM was done in a ridge-furrow system with subsurface irrigation. Soil characterization comprised soil texture and aggregate stability, soil organic carbon, pH and water content. Soil microbial analysis included: Soil microbial biomass (Cmic), a fraction of soil cultivable fungi (CFU values), soil bacteria (16S rRNA), denitrifying community (nirK, nirS, narG, napA genes), soil enzyme activity (C-Chitinase, P-Phosphatase and N Leucine-aminopeptidase), deoxynivalenol (DON) content and Cmic:Corg ratio. Positive effects on soil physicochemical properties were observed under PM as compared to SM, reflected by a higher soil carbon content and better aggregate stability (p>0.05). Yet, soil microbial analysis revealed some differences between managements. Cmic values were comparable in both systems, showing no differences in soil microbial biomass. In the same way, the analysis of functional genes of the N cycle and the activity of the enzymes P-Phosphatase and N Leucine-aminopeptidase was not affected by the mulching treatment. But, the abundance of bacteria (18%) and a fraction of soil cultivable fungi were reduced by respectively 18 and 62% under PM. Since the Cmic values remained similar between treatments, this accounts for a shift of microbial communities under PM. Additionally, C-Chitinase activity declined under PM. Interestingly, this enzyme correlated positively with CFU values (r=0.781, p=0.001), suggesting that a reduction of the activity is a consequence of the reduction of the fungal biomass. Additionally, a higher deoxynivalenol concentration (2.2 ± 2.4 µg kg-1) and a reduced Cmic:Corg ratio (1.3±0.3%) were observed under PM, indicative of less appropriate soil conditions after long-term PM management

Ecophysiology of Aspergillus Section Nigri Species Potential Ochratoxin A Producers

After aflatoxins, ochratoxin A (OTA) is the most studied mycotoxin due to the toxicological significance in human and animal diets. OTA presence has been extensively reported worldwide in the last decade in several agricultural products. The main OTA producer in tropical and temperate climates is Aspergillus carbonarius followed by species belonging to A. niger aggregate. Currently, many scientists worldwide have studied the influence of water activity and temperature for growth and biosynthesis of OTA by these species on synthetic media. This article reviews ecophysiological studies of Aspergillus section Nigri strains on synthetic media and natural substrates. The results of these investigations suggest that significant amounts of OTA can be produced in only five days and that the use of different storage practices, such as aW and temperature levels below 0.930 and 15 °C, respectively, allow controlling fungal contamination and minimizing the OTA production in several products as peanuts, corn, dried grapes and derived products for human consumption

Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins

Species in the genus Aspergillus have been classified primarily based on morphological features. Sequencing of house-hold genes has also been used in Aspergillus taxonomy and phylogeny, while extrolites and physiological features have been used less frequently. Three independent ways of classifying and identifying aspergilli appear to be applicable: Morphology combined with physiology and nutritional features, secondary metabolite profiling and DNA sequencing. These three ways of identifying Aspergillus species often point to the same species. This consensus approach can be used initially, but if consensus is achieved it is recommended to combine at least two of these independent ways of characterising aspergilli in a polyphasic taxonomy. The chemical combination of secondary metabolites and DNA sequence features has not been explored in taxonomy yet, however. Examples of these different taxonomic approaches will be given for Aspergillus section Nigri

Beyond equilibrium climate sensitivity

ISSN:1752-0908ISSN:1752-089

Modelling the relationship between environmental factors, transcriptional genes and deoxynivalenol mycotoxin production by strains of two Fusarium species

The effect of changes in temperature/water activity (a(w)) on growth, deoxynivalenol (DON) production and trichothecene gene cluster expression (18 genes) for strains of Fusarium culmorum and Fusarium graminearum was studied. The expression data for six key transcription genes (TRI4, TRI5, TRI6, TRI10, TRI12 and TRI13) were analysed using multiple regression analyses to model the relationship between these various factors for the first time. Changes in a(w) and temperature significantly (p=0.05) affected growth and DON. Microarray data on expression of these genes were significantly related to DON production for both strains. Multi-regression analysis was done and polynomial models found to best fit the relationship between actual/predicted DON production relative to the expression of these TRI genes and environmental factors. This allowed prediction of the amounts of DON produced in two-dimensional contour maps to relate expression of these genes to either a(w) or temperature. These results suggest complex interactions between gene expression (TRI genes), environmental factors and mycotoxin production. This is a powerful tool for understanding the role of these genes in relation to environmental factors and enables more effective targeted control strategies to be developed

A systems approach to model the relationship between aflatoxin gene cluster expression, environmental factors, growth and toxin production by Aspergillus flavus.

A microarray analysis was used to examine the effect of combinations of water activity (a(w), 0.995-0.90) and temperature (20-42°C) on the activation of aflatoxin biosynthetic genes (30 genes) in Aspergillus flavus grown on a conducive YES (20 g yeast extract, 150 g sucrose, 1 g MgSO(4)·7H(2)O) medium. The relative expression of 10 key genes (aflF, aflD, aflE, aflM, aflO, aflP, aflQ, aflX, aflR and aflS) in the biosynthetic pathway was examined in relation to different environmental factors and phenotypic aflatoxin B(1) (AFB(1)) production. These data, plus data on relative growth rates and AFB(1) production under different a(w) × temperature conditions were used to develop a mixed-growth-associated product formation model. The gene expression data were normalized and then used as a linear combination of the data for all 10 genes and combined with the physical model. This was used to relate gene expression to a(w) and temperature conditions to predict AFB(1) production. The relationship between the observed AFB(1) production provided a good linear regression fit to the predicted production based in the model. The model was then validated by examining datasets outside the model fitting conditions used (37°C, 40°C and different a(w) levels). The relationship between structural genes (aflD, aflM) in the biosynthetic pathway and the regulatory genes (aflS, aflJ) was examined in relation to a(w) and temperature by developing ternary diagrams of relative expression. These findings are important in developing a more integrated systems approach by combining gene expression, ecophysiological influences and growth data to predict mycotoxin production. This could help in developing a more targeted approach to develop prevention strategies to control such carcinogenic natural metabolites that are prevalent in many staple food products. The model could also be used to predict the impact of climate change on toxin production

Effect of Kluyveromyces thermotolerans on polyketide synthase gene expression and ochratoxin accumulation by Penicillium and Aspergillus

In a previous study, it was demonstrated that Kluyveromyces thermotolerans strains can reduce both growth and ochratoxin A (OTA) accumulation by Aspergillus section Nigri strains. There is no information about the mechanisms related to this reduction. A viable hypothesis can be that the presence of biocontrol agents can affect OTA biosynthesis by influencing the transcriptional activity of the polyketide synthase (pks) gene, one of the key enzymes in the OTA biosynthetic pathway. The aims of this work were to determine the effect of two selected strains of K. thermotolerans as potential biocontrol agents and to evaluate if their presence can affect the otapks gene expression of ochratoxigenic Aspergillus and Penicillium species. Growth, OTA and ochratoxin B (OTB) biosynthesis by the fungal strains at the phenotypic and molecular levels were monitored. The results obtained showed that both K. thermotolerans strains evaluated had a strong influence on growth, OTA and OTB biosynthesis, and expression of the mycotoxin biosynthesis genes. However, no direct correlation between the influence of the biocontrol yeasts on pks gene expression, OTA and OTB production could be found. These results could indicate an inhibitory mechanism by the yeasts, which apparently involve a post-transcriptional mechanism. The data obtained could imply that the production of mycotoxins can be regarded as a kind of adaptation mechanism to environmental stress conditions by these mycotoxigenic species.Fil: Ponsone, Maria Lorena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kuhn, Yamila Gisele. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schmidt Heydt, M.. Max Rubner Institut; AlemaniaFil: Geisen, R.. Max Rubner Institut; AlemaniaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin