222 research outputs found

    Quantitative trait loci for head-bug resistance in sorghum

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    QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14 microsatellite markers gave usable amplification products. A genetic map including 92 loci distributed over 13 linkage groups, and covering a total distance of 1160 cM was built. Three significant and seven putative QTLs were detected and placed on the ma

    Quantitative trait loci for head-bug resistance in sorghum

    Get PDF
    QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14 microsatellite markers gave usable amplification products. A genetic map including 92 loci distributed over 13 linkage groups, and covering a total distance of 1160 cM was built. Three significant and seven putative QTLs were detected and placed on the ma

    Quantitative trait loci for head-bug resistance in sorghum

    Get PDF
    QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14 microsatellite markers gave usable amplification products. A genetic map including 92 loci distributed over 13 linkage groups, and covering a total distance of 1160 cM was built. Three significant and seven putative QTLs were detected and placed on the map

    Quantitative trait loci for head-bug resistance in sorghum

    Get PDF
    QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14 microsatellite markers gave usable amplification products. A genetic map including 92 loci distributed over 13 linkage groups, and covering a total distance of 1160 cM was built. Three significant and seven putative QTLs were detected and placed on the map

    Quantitative trait loci for head bug resistance in sorghum

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    An experiment was conducted during the 1997/99 cropping seasons, in Mali, France, to study the inheritance for resistance to head bug (Eurystylus oldi) of sorghum progenies using quantitative trait loci (QTL) maps. Cultivars Malisor 84-7 (resistant) and S 34 (susceptible) were used as parents. To build the sorghum genetic map, 345 restriction fragment length polymorphism (RFLP) probes in combination with 6 restriction enzymes (BamHI, DraI, EcoRI, EcoRV, HindIII and SstI) were screened for their ability to reveal polymorphism. Thousand kernel weight (TKW) and germination rate at grain maturity were measured. Head bug damage was assessed visually on a 1 to 9 scale on the infested part of the panicle (NOTF2). Among the 345 RFLP probes, 81 revealed polymorphism between the parents. Additionally, 14 microsatellite markers gave amplification products. The genetic map based on Malisor 84-7 × S 34 includes 13 linkage groups (LGs) covering a total distance of 1160 cM. Three significant and 7 putative QTLs were detected. One QTL for TKW reduction that accounted for 13% of the phenotypic variation was detected on LG C2. For this QTL, resistance is conditioned by the Malisor 84-7 allele and is dominant. Two QTLs were detected on LG D and LG E for visual damage score under natural head bug infestation, explaining 16 and 26% of the phenotypic variation for this trait, respectively. Resistance from the QTL on LG D is conditioned by the S 34 allele, whereas resistance from the QTL on LG E is provided by the Malisor 84-7 allele. In both cases, resistance is recessive. No significant QTLs were detected for NOTF2 and germination rate, but co-localization of 2 putative QTLs for these traits was observed on LG G2, and in both cases, resistance is conditioned by the S 34 allele

    Patterns of Chemical Diversity in the Mediterranean Sponge Spongia lamella

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    The intra-specific diversity in secondary metabolites can provide crucial information for understanding species ecology and evolution but has received limited attention in marine chemical ecology. The complex nature of diversity is partially responsible for the lack of studies, which often target a narrow number of major compounds. Here, we investigated the intra-specific chemical diversity of the Mediterranean sponge Spongia lamella. The chemical profiles of seven populations spreading over 1200 km in the Western Mediterranean were obtained by a straightforward SPE-HPLC-DAD-ELSD process whereas the identity of compounds was assessed by comparison between HPLC-MS spectra and literature data. Chemical diversity calculated by richness and Shannon indexes differed significantly between sponge populations but not at a larger regional scale. We used factor analysis, analysis of variance, and regression analysis to examine the chemical variability of this sponge at local and regional scales, to establish general patterns of variation in chemical diversity. The abundance of some metabolites varied significantly between sponge populations. Despite these significant differences between populations, we found a clear pattern of increasing chemical dissimilarity with increasing geographic distance. Additional large spatial scale studies on the chemical diversity of marine organisms will validate the universality or exclusivity of this pattern

    The Northern Route for Human dispersal in Central and Northeast Asia: New evidence from the site of Tolbor-16, Mongolia.

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    The fossil record suggests that at least two major human dispersals occurred across the Eurasian steppe during the Late Pleistocene. Neanderthals and Modern Humans moved eastward into Central Asia, a region intermittently occupied by the enigmatic Denisovans. Genetic data indicates that the Denisovans interbred with Neanderthals near the Altai Mountains (South Siberia) but where and when they met H. sapiens is yet to be determined. Here we present archaeological evidence that document the timing and environmental context of a third long-distance population movement in Central Asia, during a temperate climatic event around 45,000 years ago. The early occurrence of the Initial Upper Palaeolithic, a techno-complex whose sudden appearance coincides with the first occurrence of H. sapiens in the Eurasian steppes, establishes an essential archaeological link between the Siberian Altai and Northwestern China . Such connection between regions provides empirical ground to discuss contacts between local and exogenous populations in Central and Northeast Asia during the Late Pleistocene
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