Hamster-to-rat heart and liver xenotransplantation with FK506 plus antiproliferative drugs

Heterotopic hamster hearts transplanted to unmodified LEW rats underwent humoral rejection in 3 days. Survival was prolonged to a median of 4 days with 2 mg/kg/day FK506. As monotherapy, 15 mg/kg/day cyclophosphamide greatly prolonged graft survival-far more than could be accomplished with RS-61443, brequinar (BQR), mizoribine, methotrexate, or deoxyspergualin. However, when FK506 treatment, which was ineffective alone, was combined with a short induction course (14 or 30 days) of subtherapeutic BQR, RS-61443, or cyclophosphamide, routine survival of heart xenografts was possible for as long as the daily FK506 was continued. In addition, a single large dose of 80 mg/kg cyclophosphamide 10 days preoperatively allowed routine cardiac xenograft survival under FK506. The ability of these antimetabolites to unmask the therapeutic potential of FK506 correlated, although imperfectly, with the prevention of rises of preformed heterospecific cytotoxic antibodies immediately postoperatively. As an adjunct to FK506, azathioprine was of marginal value, whereas mizoribine, methotrexate, and deoxyspergualin (DSPG) were of intermediate efficacy. After orthotopic hepatic xenotransplantation, the perioperative survival of the liver with its well-known resistance to antibodies was less dependent than the heart on the antimetabolite component of the combined drug therapy, but the unsatisfactory results with monotherapy of FK506, BQR, RS-61443, or cyclophosphamide were changed to routine success by combining continuous FK506 with a short course of any of the other drugs. Thus, by breaking down the antibody barrier to xenotransplantation with these so-called antiproliferative drugs, it has been possible with FK506 to transplant heart and liver xenografts with consistent long-term survival of healthy recipients

Curie Temperatures and Emplacement Conditions of Pyroclastic Deposits From Popocatépetl Volcano, Mexico

Most pyroclastic deposits of Popocatépetl volcano were emplaced at high temperatures and have similar mafic to more evolved compositions, suggesting a long-lived, interconnected magma environment. We performed a magnetic and microscopic study on different eruptive sequences <14 ky in age and found that temperature and field dependence of magnetic susceptibility are suited to separate eruption phases. We observed homogeneous titanomagnetite with Curie temperatures (T$_{C}$) of 50–200°C and 200–400°C, together with different amounts of oxy-exsolved titanomagnetite with T$_{C}$ ∼ 570°C. Some block-and-ash flow deposits show remarkably irreversible T$_{C}$ in heating and cooling branches with a positive ΔT$_{C}$ (T$_{C}$ $_{heating}$ –T$_{C}$ $_{cooling}$) of up to 130°C in the center. The central part of this sequence is characterized by decreasing magnetic susceptibility and low field dependence of magnetic susceptibility (<10%), which is atypical for ulvöspinel-rich titanomagnetite. The nonreversibility of heating and cooling runs measured with rates of around 10 K/min is probably related to vacancy-enhanced nanoscale chemical clustering, which seems to occur preferentially during rapid quenching, possibly combined with subtle maghemitization. In contrast, pumice layers have the highest field dependence (∼20%) and contain Ti-rich and intermediate titanomagnetite with T$_{C}$ < 100 and ∼300°C, which are in line with mafic and more evolved magma composition. In intermediate phases, irreversibility of T$_{C}$ is more common but with a relatively low ΔT$_{C}$ of ±20°C. We suggest that magneto-mineralogy in pyroclastic density currents is complex but offers a complementary tool to the paleomagnetic directional analysis for emplacement temperature and contribute information on the volcanic material history and their emplacement conditions

Stability of axially symmetric magnetic fields in stars

The magnetic fields observed in Ap-stars, white dwarfs, and neutron stars are known to be stable for long times. However, the physical conditions inside the stellar interiors that allow these states are still a matter of research. It has been formally demonstrated that both purely toroidal and purely poloidal magnetic fields develop instabilities at some point in the star. On the other hand, numerical simulations have proved the stability of roughly axisymmetric magnetic field configurations inside stably stratified stars. These configurations consist of mutually stabilizing toroidal and poloidal components in a twisted torus shape. Previous studies have proposed rough upper and lower bounds on the ratio of the magnetic energy in the toroidal and poloidal components of the magnetic field. With the purpose of mapping out the parameter space under which such configurations remain stable, we used the Pencil Code to perform 3D magnetohydrodynamic simulations of the evolution of the magnetic field in non-rotating, non-degenerate stars in which viscosity is the only dissipation mechanism, both for stars with a uniform (barotropic) and radially increasing (stably stratified) specific entropy. Furthermore, we considered different conditions regarding the degree of stable stratification and the magnetic energy in each component, roughly confirming the previously suggested stability boundaries for the magnetic field.Comment: 9 pages, 9 figure

Seed systems and crop genetic diversity in agroecosystems

Poster presented at the First Diversitas Open Science Conference. Oaxaca (Mexico), 9-12 Nov 200

STING-dependent recognition of cyclic di-AMP mediates type I interferon responses during Chlamydia trachomatis infection.

UnlabelledSTING (stimulator of interferon [IFN] genes) initiates type I IFN responses in mammalian cells through the detection of microbial nucleic acids. The membrane-bound obligate intracellular bacterium Chlamydia trachomatis induces a STING-dependent type I IFN response in infected cells, yet the IFN-inducing ligand remains unknown. In this report, we provide evidence that Chlamydia synthesizes cyclic di-AMP (c-di-AMP), a nucleic acid metabolite not previously identified in Gram-negative bacteria, and that this metabolite is a prominent ligand for STING-mediated activation of IFN responses during infection. We used primary mouse lung fibroblasts and HEK293T cells to compare IFN-β responses to Chlamydia infection, c-di-AMP, and other type I IFN-inducing stimuli. Chlamydia infection and c-di-AMP treatment induced type I IFN responses in cells expressing STING but not in cells expressing STING variants that cannot sense cyclic dinucleotides but still respond to cytoplasmic DNA. The failure to induce a type I IFN response to Chlamydia and c-di-AMP correlated with the inability of STING to relocalize from the endoplasmic reticulum to cytoplasmic punctate signaling complexes required for IFN activation. We conclude that Chlamydia induces STING-mediated IFN responses through the detection of c-di-AMP in the host cell cytosol and propose that c-di-AMP is the ligand predominantly responsible for inducing such a response in Chlamydia-infected cells.ImportanceThis study shows that the Gram-negative obligate pathogen Chlamydia trachomatis, a major cause of pelvic inflammatory disease and infertility, synthesizes cyclic di-AMP (c-di-AMP), a nucleic acid metabolite that thus far has been described only in Gram-positive bacteria. We further provide evidence that the host cell employs an endoplasmic reticulum (ER)-localized cytoplasmic sensor, STING (stimulator of interferon [IFN] genes), to detect c-di-AMP synthesized by Chlamydia and induce a protective IFN response. This detection occurs even though Chlamydia is confined to a membrane-bound vacuole. This raises the possibility that the ER, an organelle that innervates the entire cytoplasm, is equipped with pattern recognition receptors that can directly survey membrane-bound pathogen-containing vacuoles for leaking microbe-specific metabolites to mount type I IFN responses required to control microbial infections

Coeducational ideas In physical education teachers: Pshycometric properties of a scale

Ante la falta de cuestionarios o escalas que evaluaran los diferentes aspectos coeducativos que caracterizan al profesorado de Educación Física y la necesidad de conocer cuál es la concepción que tienen sobre este modelo, se desprende el objetivo de este trabajo, como el de evaluar las propiedades psicométricas mediante un instrumento que hemos denominado Escala sobre el pensamiento coeducativo del profesorado de EF para valorar las opiniones respecto a la coeducación y la metodología que se utiliza en sus clases. La muestra estuvo compuesta por 213 profesores, 133 hombres y 43 mujeres. La escala fue aplicada mediante papel, envío de correo electrónico y plataforma moddle. El análisis de los datos muestra unos resultados adecuados en cuanto a estructura factorial, consistencia interna y tipos de validez. Se concluye que esta Escala representa un instrumento válido y fiable para analizar las características coeducativas del profesoradoBecause of the absence of questionnaires or scales which will assess different coeducative aspects that are characteristics of PE teacher and need to know which is the conception that they have about this model, it is deduced the aim of this work as that which evaluate the psychometric properties through a tool called the Scale of coeducational ideas in physical education teachers to value the opinions about coeducation and the methodology used in their classes. The sample was composed by 213 teachers, 133 men and 43 women. The questionnaire was applied by means of paper, electronic mail shipment and platform moddle. The data analysis shows appropriate results in terms of factor structure, internal consistency and validity types. We conclude that the escale represents a valid and reliable instrument to analyze the coeducative characteristics of the teacher

What can metazoan parasites reveal about the taxonomy of Scomber japonicus Houttuyn in the coast of South America and Madeira Islands?

The metazoan parasites of four populations of the chub mackerel Scomber japonicus were analysed from two localities in the Atlantic Ocean (Madeira Islands, Portugal, and Rio de Janeiro, Brazil) and two localities in the Pacific Ocean (Callao, Peru, and Antofagasta, Chile), collected during 2002 and 2003. A total of 373 fish specimens were studied and 34 metazoan parasite species were obtained. Parasites identified from the populations of chub mackerel studied could be separated into three categories: parasites with a wide distribution, present in the Pacific and Atlantic, parasites proper of the Pacific Ocean and parasites proper of the Atlantic Ocean. The analyses of some highly specific parasites of the genus Scomber (i.e. monogeneans of the genus Kuhnia and didymozoid digeneans) strongly suggest the need for a revision of the taxonomic status of chub mackerels from the Atlantic and Pacific coast of America. The results demonstrated the usefulness of parasites as adequate tools to clarify the taxonomic status of their hosts.We thank R. Poulin (University of Otago, New Zealand) for reading and commenting on an early draft of the manuscript. J.L.L. was supported by a fellowship (N° 200906-2005.4) of the Conselho Nacional de Desenvolvimento Cientı´fico e Tecnolo´ gico (CNPq) of Brazil for postdoctoral research in the Department of Zoology at the University of Otago, New Zealand. G. Costa was supported by Portuguese Foundation of Science and Technology, programme POCTI.info:eu-repo/semantics/publishedVersio

TIA: A forward model and analyzer for Talbot interferometry experiments of dense plasmas

Producción CientíficaInterferometry is one of the most sensitive and successful diagnostic methods for plasmas. However, owing to the design of most common interferometric systems, the wavelengths of operation and, therefore, the range of densities and temperatures that can be probed are severely limited. Talbot–Lau interferometry offers the possibility of extending interferometry measurements to x-ray wavelengths by means of the Talbot effect. While there have been several proof-of-concept experiments showing the efficacy of this method, it is only recently that experiments to probe High Energy Density (HED) plasmas using Talbot–Lau interferometry are starting to take place. To improve these experimental designs, we present here the Talbot-Interferometry Analyzer (TIA) tool, a forward model for generating and postprocessing synthetic x-ray interferometry images from a Talbot–Lau interferometer. Although TIA can work with any two-dimensional hydrodynamic code to study plasma conditions as close to reality as possible, this software has been designed to work by default with output files from the hydrodynamic code FLASH, making the tool user-friendly and accessible to the general plasma physics community. The model has been built into a standalone app, which can be installed by anyone with access to the MATLAB runtime installer and is available upon request to the authors