Induction of Apoptosis and Cell Cycle Arrest in Human Colon Carcinoma Cells by Corema album Leaves

The leaves of Corema album (Ericaceae), an endemic shrub which grows in Atlantic coastal areas of the Iberian Peninsula, are rich in flavonoids and other secondary metabolites. Silica gel column chromatography of the ethyl acetate extract from dried leaves was performed and a flavonic active fraction was obtained. The cytotoxic activity of this fraction was assessed using the colon cancer cell lines HCT116 and HT29. After 48 hours of treatment, cell viability was determined with luminescence-based ATPLite assay, showing IC50 values of 7.2 ± 0.7 and 6.8 ± 1.2 µg/mL, respectively. The study by flow cytometry revealed that the cytotoxicity of this fraction was mediated, at least in part, by induction of apoptosis and G2/M cell cycle arrest. The active fraction was then subjected to Sephadex LH-20 chromatography and two flavonoids were separated and identified as the flavanone pinocembrin and 2’,4’-dihydroxychalcone after UV, MS and NMR analysis

Agree or disagree: Does it matter which comes first? An examination of scale direction effects in a multi-device online survey

Previous research shows that the direction of rating scales can influence participants’ response behavior. Studies also suggest that the device used to complete online surveys might affect the susceptibility to these effects due to the different question layouts (e.g., horizontal grids vs. vertical individual questions). This article contributes to previous research by examining scale direction effects in an online multi-device survey conducted with panelists in Spain. In this experiment, respondents were randomly assigned to two groups where the scale direction was manipulated (incremental vs. decre mental). Respondents completed the questionnaire using the device of their choosing (57.8% used PCs; 36.5% used smartphones; and 5.7% used tablets). The results show that scale direction influenced response distributions but did not significantly affect data quality. In addition, our findings indicate that scale direction effects were comparable across devices. Findings are dis cussed and implications are highlighted. Survey research has proved that many design features of scales affect how respondents process and use them to construct their responses (DeCastellarnau 2018; Menold and Bogner 2016; Yan and Keusch 2015). For instance, it is well established that the presentation order of categorical response options influences survey responses. This type of response bias, known as response-order effects, distinguishes between primacy and recency effects (Chang and Krosnick 2009; Galesic et al.2008; Stern et al. 2007). Primacy effects refer to higher endorsements of response categories presented early in the list, while recency effects refer to higher endorsements of response categories presented later in the list (Schwarz and Hippler 2004). Although previous research has widely documented the impact on sur vey responses produced by varying the order of categorical response options, fewer studies have examined response-order effects with ordinal scales, despite their extensive use in survey research. The first studies that analyzed these effects date back to the 1960s, when Belson (1966) found that survey responses tended to shift toward the starting point of the rating scales, regardless of scale length and respondent characteristics. More recent studies, however, have turned up mixed evidence. Some have reported a similar tendency for responses to be biased toward the starting point of scales (Garbarski et al. 2018; Ho¨hne and Krebs 2017; Israel 2006; Toepoel et al. 2009; Yan and Keusch 2018), while others have found no effect of the direction of rating scales on survey responses (Krebs and Hoffmeyer-Zlotnik 2010; Rammstedt and Krebs 2007; Weng and Cheng 2000). In addition, some studies have reported scale direction effects on a limited number of questions and no effect on other questions within the same experiment (Christian et al. 2009; Elway 2013; Ho¨hne and Krebs 2017

The impact of confidentiality assurances on participants’ responses to sensitive questions

Privacy, anonymity, and confidentiality are issues often addressed when collecting sensitive information (Tourangeau, 2017). Despite the importance of the concept, there is no consensus on what constitutes a sensitive question (Tourangeau, Rips, & Rasinski, 2000). According to Tourangeau and Yan (2007), a question is considered sensitive when it asks for a socially undesirable answer, requesting that the respondent admits that he or she has violated a social or legal norm. Due to the potential consequences of admitting some socially undesirable beliefs, behaviors, or activities (e.g.,social or legal sanctioning), sensitive questions are often associated with systematic measurement error (Groves et al., 2004). For instance, research has shown that sensitive questions have comparatively higher item nonresponse rates than nonsensitive questions, and they are regarded as one of the major causes for socially desirable answers in surveys(Diekmann, 2003; Krumpal, 2013; Na¨her & Krumpal, 2012; Tourangeau et al., 2000). Over the years, numerous methodological experiments have been conducted to analyze the impact of several factors on reducing measurement error in sensitive questions. Survey mode has been found to be one of the most important factors in explaining social desirability bias in sensitive questions (Holbrook, Green, & Krosnick, 2003; Tourangeau & Yan, 2007; Turner et al., 2005). It has been found that, in general, when interviewers collect the data or when they are present during data collection, responses are more socially desirable than in self-administered surveys (Kreuter, Presser, & Tourangeau, 2008; Tourangeau & Yan, 2007)

Climate change, coastal tourism, and impact chains – a literature review

Climate change impacts tourism, since both supply and demand of tourism services depend on the quality and the management of a set of environmental attributes. This paper critically reviews the empirical evidence in the literature of the last twenty years (2000–2019), by identifying the potential impacts of climate change in coastal and maritime destinations. The concept of Impact Chains is the methodological framework through which the literature is systematically selected, classified and assessed. A great heterogeneity of results is found, with estimates of physical and socio-economic impacts of climate change differing across destinations and methodologies. Moreover, the majority of recent studies mainly deals with only a few of the most important impacts, hence future research should be re-directed to overlooked indicators and relationships, which are key for designing effective climate policies at tourism destinations

Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats

Long-termchronic intermittent exposure to altitude hypoxia is a labor phenomenon requiring further research. Using a rat model, we examined whether this type of exposure differed from chronic exposure in terms of pulmonary artery remodeling and other features. Rats were subjected to chronic hypoxia (CH, = 9) and long-term intermittent hypoxia (CIH2x2; 2 days of hypoxia/2 days of normoxia, = 10) in a chamber (428 Torr, 4,600m of altitude) for 46 days and compared to rats under normoxia (NX, = 10). Body weight, hematocrit, and right ventricle ratio were measured. Pulmonary artery remodeling was assessed using confocal microscopy of tissues stained with a nuclear dye (DAPI) and CD11b antibody. Both hypoxic conditions exhibited increased hematocrit and hypertrophy of the right ventricle, tunica adventitia, and tunica media, with no changes in lumen size. The medial hypertrophy area (larger in CH) depicted a significant increase in smooth muscle cell number. Additionally, CIH2x2 increased the adventitial hypertrophy area, with an increased cellularity and a larger prevalence of clustered inflammatory cells. In conclusion, CIH2x2 elicitsmilder effects on pulmonary artery medial layermuscularization and subsequent right ventricular hypertrophy than CH. However, CIH2x2 induces greater and characteristic alterations of the adventitial layerThis work was funded by GORE-FNDR-BIP: 30125349-0, AECID Nº A/030023/10, and CYTED-ALTMEDFIS grant

Chondroid Tumors: Review of Salient Imaging Features and Update on the WHO Classification

Chondrogenic tumors are typically well recognized on radiographs, but differentiation between benign and malignant cartilaginous lesions can be difficult both for the radiologist and for the pathologist. Diagnosis is based on a combination of clinical, radiological and histological findings. While treatment of benign lesions does not require surgery, the only curative treatment for chondrosarcoma is resection. This article (1) emphasizes the update of the WHO classification and its diagnostic and clinical effects; (2) describes the imaging features of the various types of cartilaginous tumors, highlighting findings that can help differentiate benign from malignant lesions; (3) presents differential diagnoses; and (4) provides pathologic correlation. We attempt to offer valuable clues in the approach to this vast entit

A new pharmacogenetic algorithm to predict the most appropriate dosage of acenocoumarol for stable anticoagulation in a mixed Spanish population

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.There is a strong association between genetic polymorphisms and the acenocoumarol dosage requirements. Genotyping the polymorphisms involved in the pharmacokinetics and pharmacodynamics of acenocoumarol before starting anticoagulant therapy would result in a better quality of life and a more efficient use of healthcare resources. The objective of this study is to develop a new algorithm that includes clinical and genetic variables to predict the most appropriate acenocoumarol dosage for stable anticoagulation in a wide range of patients. We recruited 685 patients from 2 Spanish hospitals and 1 primary healthcare center. We randomly chose 80% of the patients (n = 556), considering an equitable distribution of genotypes to form the generation cohort. The remaining 20% (n = 129) formed the validation cohort. Multiple linear regression was used to generate the algorithm using the acenocoumarol stable dosage as the dependent variable and the clinical and genotypic variables as the independent variables. The variables included in the algorithm were age, weight, amiodarone use, enzyme inducer status, international normalized ratio target range and the presence of CYP2C9∗2 (rs1799853), CYP2C9∗3 (rs1057910), VKORC1 (rs9923231) and CYP4F2 (rs2108622). The coefficient of determination (R2) explained by the algorithm was 52.8% in the generation cohort and 64% in the validation cohort. The following R2 values were evaluated by pathology: atrial fibrillation, 57.4%; valve replacement, 56.3%; and venous thromboembolic disease, 51.5%. When the patients were classified into 3 dosage groups according to the stable dosage (<11 mg/week, 11-21 mg/week, >21 mg/week), the percentage of correctly classified patients was higher in the intermediate group, whereas differences between pharmacogenetic and clinical algorithms increased in the extreme dosage groups. Our algorithm could improve acenocoumarol dosage selection for patients who will begin treatment with this drug, especially in extreme-dosage patients. The predictability of the pharmacogenetic algorithm did not vary significantly between diseases.This study was funded by a grant from the Spanish Ministry of Health and Social Policy (Instituto de Salud Carlos III, PI07/0710) and the Andalusian Regional Ministry of Health (Progress and Health Foundation, PI-0717-2013

Consumption of fried foods and risk of coronary heart disease: Spanish cohort of the European Prospective Investigation into Cancer and Nutrition study

Objective To assess the association between consumption of fried foods and risk of coronary heart disease

Estudio para la mejora de la calidad del vino albariño

Premio de Investigación, Real Academia Galega de Ciencias, convocatoria 2009.[EN]Twenty-two clones from Albariño variety (Vitis vinifera L.), from an initial collection of 115 clones, were selected on the basis of their ampelographic, molecular and sanitary characteristics. These selected clones were studied from the agronomic and oenological point of view, and were also quantified for their levels of susceptibility to Powdery Mildew, Oidium and Botrytis. An ecotypic yeast was selected, its use has been patented and it is being exploited. Musts obtained from the previously selected Albariño clones were fermented with this yeast, essentially by increasing the content in volatile substances of interest (terpens: linalool and geraniol; norisoprenoids: α-ionone and β- damascenone), leading to wines with improved fermentative dynamic and sensorial attributes.[ES]En base a características ampelográficas, moleculares y sanitarias, se seleccionaron 22 clones de la variedad Albariño (Vitis vinifera L.), partiendo de 115 iniciales. Sobre los clones seleccionados se ha llevado a cabo un estudio agronómico y enológico, así como la cuantificación de los niveles de susceptibilidad a Mildiu, Oídio y Botrytis. Se ha seleccionado una levadura ecotípica, cuyo uso ha sido patentado y se encuentra en explotación. Con ella se fermentaron los mostos obtenidos a partir de los clones de Albariño previamente seleccionados, dando lugar a vinos con una dinámica fermentativa xPremio de Investigación, Real Academia Galega de Ciencias, convocatoria 2009 y unos atributos sensoriales mejorados, fundamentalmente en base al aumento del contenido en sustancias volátiles de interés (terpenos: linalool y geraniol; norisoprenoides: α-ionona y β- damascenona).La actividad realizada ha sido financiada, además de por la Bodega Terras Gauda S.A., por la Xunta de Galicia (PGIDIT04TAL035E), y por el propio CSIC (PIE 2004 7 0E 214).Peer reviewe