Localizationof the 5S and 45S rDNA sites and CPDNA sequence analysis in species of the quadrifaria group of Paspalum (Poaceae, Panicae).

The Quadrifaria group of Paspalum (Poaceae, Paniceae) comprises species native to the subtropical and temperate regions of South America. The purpose of this research was to characterize the I genomes in five species of this group and to establish phylogenetic relationships among them. Prometaphase chromatin condensation patterns, the physical location of 5S and 45S rDNA sites by fluorescence in situ hybridization (FISH), and sequences of five chloroplast non-coding regions were analysed. The condensation patterns observed were highly conserved among diploid and tetraploid accessions studied and not influenced by the dyes used or by the FISH procedure, allowing the identification of almost all the chromosome pairs that carried the rDNA signals. The FISH analysis of 5S rDNA sites showed the same localization and a correspondence between the number of sites and ploidy level. In contrast, the distribution of 45S rDNA sites was variable. Two general patterns were observed with respect to the location of the 45S rDNA. The species and cytotypes Paspalum haumanii 2x, P. intermedium 2x, P. quadrifarium 4x and P. exaltatum 4x showed proximal sites on chromosome 8 and two to four distal sites in other chromosomes, while P. quarinii 4x and P. quadrifarium 2x showed only distal sites located on a variable number of small chromosomes and on the long arm of chromosome 1. The single most-parsimonious tree found from the phylogenetic analysis showed the Quadrifaria species partitioned in two clades, one of them includes P. haumanii 2x and P. intermedium 2x together with P. quadrifarium 4x and P. exaltatum 4x, while the other contains P. quadrifarium 2x and P. quarinii 4x. The subdivision found with FISH is consistent with the clades recovered with cpDNA data and both analyses suggest that the Quadrifaria group, as presently defined, is not monophyletic and its species belong in at least two clades

Digitalization and artificial knowledge for accountability in SCM: a systematic literature review

Purpose: In this study, the authors examine artificial knowledge as a fundamental stream of knowledge management for sustainable and resilient business models in supply chain management (SCM). The study aims to provide a comprehensive overview of artificial knowledge and digitalization as key enablers of the improvement of SCM accountability and sustainable performance towards the UN 2030 Agenda. Design/methodology/approach: Using the SCOPUS database and Google Scholar, the authors analyzed 135 English-language publications from 1990 to 2022 to chart the pattern of knowledge production and dissemination in the literature. The data were collected, reviewed and peer-reviewed before conducting bibliometric analysis and a systematic literature review to support future research agenda. Findings: The results highlight that artificial knowledge and digitalization are linked to the UN 2030 Agenda. The analysis further identifies the main issues in achieving sustainable and resilient SCM business models. Based on the results, the authors develop a conceptual framework for artificial knowledge and digitalization in SCM to increase accountability and sustainable performance, especially in times of sudden crises when business resilience is imperative. Research limitations/implications: The study results add to the extant literature by examining artificial knowledge and digitalization from the resilience theory perspective. The authors suggest that different strategic perspectives significantly promote resilience for SCM digitization and sustainable development. Notably, fostering diverse peer exchange relationships can help stimulate peer knowledge and act as a palliative mechanism that builds digital knowledge to strengthen and drive future possibilities. Practical implications: This research offers valuable guidance to supply chain practitioners, managers and policymakers in re-thinking, re-formulating and re-shaping organizational processes to meet the UN 2030 Agenda, mainly by introducing artificial knowledge in digital transformation training and education programs. In doing so, firms should focus not simply on digital transformation but also on cultural transformation to enhance SCM accountability and sustainable performance in resilient business models. Originality/value: This study is, to the authors' best knowledge, among the first to conceptualize artificial knowledge and digitalization issues in SCM. It further integrates resilience theory with institutional theory, legitimacy theory and stakeholder theory as the theoretical foundations of artificial knowledge in SCM, based on firms' responsibility to fulfill the sustainable development goals under the UN's 2030 Agenda

High temperature and humidity affect pollen viability and longevity in Olea europaea L.

Olea europaea L. is a crop typical of the Mediterranean area that has an important role in economy, society, and culture of this region. Climate change is expected to have significant impact on this crop, which is typically adapted to certain pedo-climatic characteristics of restricted geographic areas. In this scenario, the aim of this study was to evaluate the time-course response of pollen viability to different combinations of temperature and humidity. The study was performed comparing flowering time and pollen functionality of O. europaea from twelve cultivars growing at the same site belonging to the Campania olive collection in Italy. Pollen was incubated at 12◦C, 22◦C, and 36◦C in combination with 50% RH or 100% RH treatments for 5 days. The results highlighted that a drastic loss of pollen viability occurs when pollen is subjected to a combination of high humidity and high temperature, whereas 50% RH had less impact on pollen thermotolerance, because most cultivars preserved a high pollen viability over time. In the ongoing climate change scenario, it is critical to assess the effect of increasing temperatures on sensitive reproductive traits such as pollen viability to predict possible reduction in crop yield. Moreover, the results highlighted that the effect of temperature increase on pollen thermotolerance should be evaluated in combination with other environmental factors such as humidity conditions. The screening of olive cultivars based on pollen thermotolerance is critical in the ongoing climate change scenario, especially considering that the economic value of this species relies on successful fertilization and embryo development, and also that production cycle of Olea europaea can be longer than a hundred years

Variations of peel essential oils during fruit ripening in four lemon (Citrus limon (L.) Burm. F.) cultivars

Lemon processing procedures yield a significant amount of waste as peels, which are 57% of processed lemons and represent a possible source of bioactive compounds (essential oils, EOs). EOs were extracted from lemon fruits belonging to four cultivars harvested at four different sampling times (25 October, 23 November, 20 December, 1 February), characterized, and quantified through gas chromatography-mass spectrometry

Análises citogenéticas em Paspalum L. revelam novas espécies e acessos diplóides

Se contaron los números de cromosomas en 126 nuevas accesiones de 50 especies de Paspalum de Brasil, Argentina, Paraguay y Bolivia. Se confirmaron los números de cromosomas 2n = 12, 20, 24, 30, 40, 50, 60, 80. Números de cromosomas para P. arenarium (2n = 20), P. barretoi (2n = 20), P. aff. ceresia (2n = 40), P. corcovadense (2n = 20), P. crispulum (2n = 20), P. flaccidum (2n = 40), P. nummularium (2n = 20), P. scalare (2n = 20) ), P. vescum (2n = 20) y P. rectum (2n = 20) y un citotipo diploide de P. malacophyllumse informan por primera vez. Se confirmó el predominio de accesiones tetraploides (43,6%), pero se encontró un número inusualmente alto de especies diploides (44%) y accesiones (35,7%). Estos resultados abren nuevas perspectivas para programas de mejoramiento, estudios filogenéticos y para la investigación sobre el control de la apomixis, ya que los diploides de Paspalum son típicamente sexuales.O número cromossômico foi determinado para 126 novos acessos de 50 espécies de Paspalum do Brasil, Argentina, Paraguai e Bolívia. Foram verificados os números somáticos 2n = 12, 20, 24, 30, 40, 50, 60 e 80. Estas são as primeiras contagens para P. arenarium (2n = 20), P. barretoi (2n = 20), P. aff . ceresia (2n = 40), P. corcovadense (2n = 20), P. crispulum (2n = 20), P. flaccidum (2n = 40), P. nummularium (2n = 20), P. scalare (2n = 20) ), P. vescum (2n = 20) y P. recto (2n = 20). O nível diplóide (2n = 20) é reportado pela primeira vez para P. malacophyllum. Os dados confirmam a predominância de acessos tetraplóides (43,6%) no gênero e mostram um número incomumente elevado de espécies (44%) e acessos diplóides (35,7%). Estes resultados trazem novas perspectivas para programas de melhoramento, para estudos filogenéticos e para pesquisa orientada ao controle da apomixia, já que em Paspalum as plantas diplóides são tipicamente sexuais.Fil: Pozzobon, Marisa Toniolo. Parque Estação Biológica; BrasilFil: Carvalho, Ana Caroline Machado De. Universidade do Brasília; BrasilFil: Vaio, Magdalena. Universidad de la República; UruguayFil: Valls, José Francisco M.. Parque Estação Biológica; BrasilFil: Peñaloza, Andréa Del Pilar De Souza. Parque Estação Biológica; BrasilFil: Santos, Sileuza Dos. Parque Estação Biológica; BrasilFil: Côrtes, Ana Luiza. Centro Universitário de Brasília; BrasilFil: Rua, Gabriel Hugo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Botánica Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Chemical Composition of PM10 at Urban Sites in Naples (Italy)

Here, we report the chemical characterization and identification of the possible sources of particulate matter (fraction PM10) at two different sites in Naples. PM10 concentration and its chemicalcompositionwerestudiedusingthecrustalenrichmentfactor(EF)andprincipalcomponent analysis (PCA). In all of the seasons, the PM10 levels, were significantly higher (p 0.8) was obtained between reconstructed mass and gravimetric mass. PCA analysis explained 76% and 79% of the variance in NA01 and NA02, respectively. The emission sources were the same for both sites; but, the location of the site, the different distances from the sources and the presence and absence of vegetation proved the different concentrations and compositions of PM10

Interactions between rootstock, inter-stem and scion xylem vessel characteristics of peach trees growing on rootstocks with contrasting size-controlling characteristics

This paper documents that while characteristics of the xylem anatomy and calculated hydraulic conductance of peach rootstock genotypes differ according to their effects on vigour of the scion they do not strongly influence the xylem characteristics of the scion. Furthermore xylem characteristics of a dwarfing rootstock genotype used as an inter-stem do not substantially influence anatomical characteristics of a vigorous rootstock below the inter-stem or the scion above it