Currents between tethered electrodes in a magnetized laboratory plasma

Laboratory experiments on important plasma physics issues of electrodynamic tethers were performed. These included current propagation, formation of wave wings, limits of current collection, nonlinear effects and instabilities, charging phenomena, and characteristics of transmission lines in plasmas. The experiments were conducted in a large afterglow plasma. The current system was established with a small electron-emitting hot cathode tethered to an electron-collecting anode, both movable across the magnetic field and energized by potential difference up to V approx.=100 T(sub e). The total current density in space and time was obtained from complete measurements of the perturbed magnetic field. The fast spacecraft motion was reproduced in the laboratory by moving the tethered electrodes in small increments, applying delayed current pulses, and reconstructing the net field by a linear superposition of locally emitted wavelets. With this technique, the small-amplitude dc current pattern is shown to form whistler wings at each electrode instead of the generally accepted Alfven wings. For the beam electrode, the whistler wing separates from the field-aligned beam which carries no net current. Large amplitude return currents to a stationary anode generate current-driven microinstabilities, parallel electric fields, ion depletions, current disruptions and time-varying electrode charging. At appropriately high potentials and neutral densities, excess neutrals are ionized near the anode. The anode sheath emits high-frequency electron transit-time oscillations at the sheath-plasma resonance. The beam generates Langmuir turbulence, ion sound turbulence, electron heating, space charge fields, and Hall currents. An insulated, perfectly conducting transmission line embedded in the plasma becomes lossy due to excitation of whistler waves and magnetic field diffusion effects. The implications of the laboratory observations on electrodynamic tethers in space are discussed

Laboratory experiments on current flow between stationary and moving electrodes in magnetoplasmas

Laboratory experiments were performed in order to investigate the basic physics of current flow between tethered electrodes in magnetoplasmas. The major findings are summarized. The experiments are performed in an effectively very large laboratory plasma in which not only the nonlinear current collection is addressed but also the propagation and spread of currents, the formation of current wings by moving electrodes, the current closure, and radiation from transmission lines. The laboratory plasma consists of a pulsed dc discharge whose Maxwellian afterglow provides a quiescent, current-free uniform background plasma. Electrodes consisting of collectors and electron emitters are inserted into the plasma and a pulsed voltage is applied between two floating electrodes via insulated transmission lines. Besides the applied current in the wire, the total current density in the plasma is obtained from space and time resolved magnetic probe measurements via Maxwell's law. Langmuir probes yield the plasma parameters

Road blocks on paleogenomes - polymerase extension profiling reveals the frequency of blocking lesions in ancient DNA

Although the last few years have seen great progress in DNA sequence retrieval from fossil specimens, some of the characteristics of ancient DNA remain poorly understood. This is particularly true for blocking lesions, i.e. chemical alterations that cannot be bypassed by DNA polymerases and thus prevent amplification and subsequent sequencing of affected molecules. Some studies have concluded that the vast majority of ancient DNA molecules carry blocking lesions, suggesting that the removal, repair or bypass of blocking lesions might dramatically increase both the time depth and geographical range of specimens available for ancient DNA analysis. However, previous studies used very indirect detection methods that did not provide conclusive estimates on the frequency of blocking lesions in endogenous ancient DNA. We developed a new method, polymerase extension profiling (PEP), that directly reveals occurrences of polymerase stalling on DNA templates. By sequencing thousands of single primer extension products using PEP methodology, we have for the first time directly identified blocking lesions in ancient DNA on a single molecule level. Although we found clear evidence for blocking lesions in three out of four ancient samples, no more than 40% of the molecules were affected in any of the samples, indicating that such modifications are far less frequent in ancient DNA than previously thought

Prospects for probing the gluon density in protons using heavy quarkonium hadroproduction

We examine carefully bottomonia hadroproduction in proton colliders, especially focusing on the LHC, as a way of probing the gluon density in protons. To this end we develop some previous work, getting quantitative predictions and concluding that our proposal can be useful to perform consistency checks of the parameterization sets of different parton distribution functions.Comment: LaTeX, 14 pages, 6 EPS figure

Post-functionalization of drug-loaded nanoparticles prepared by polymerization-induced self-assembly (PISA) with mitochondria targeting ligands

Herein, the postfunctionalization of different non-fouling PISA particles, prepared from either poly(oligo ethylene glycol methyl ether methacrylate) (pPEGMA) and the anticancer drug PENAO (4-(N-(S-penicillaminylacetyl)amino)phenylarsenonous acid) or zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and PENAO were reported. Both PISA particles were reacted with triphenylphosphonium (TPP) as mitochondria targeting units in order to evaluate the changes in cellular uptake or the toxicity of the conjugated arsenic drug. Attachment of TPP onto the PISA particles however was found not to enhance the mitochondrial accumulation, but it did influence overall the biological activity of pMPC-based particles in 2D and 3D cultured sarcoma SW982 cells. When TPP was conjugated to the pMPC PISA particles more cellular uptake as well as better spheroid penetration were observed, while TPP on PEG-based PISA had only little effect. It was hypothesized that TPP on the micelle surface may not be accessible enough to allow mitochondria targeting, but more structural investigations are required to elucidate this

freeIbis: An efficient basecaller with calibrated quality scores for Illumina sequencers

Motivation: The conversion of the raw intensities obtained from next-generation sequencing platforms into nucleotide sequences with well-calibrated quality scores is a critical step in the generation of good sequence data. While recent model-based approaches can yield highly accurate calls, they require a substantial amount of processing time and/or computational resources. We previously introduced Ibis, a fast and accurate basecaller for the Illumina platform. We have continued active development of Ibis to take into account developments in the Illumina technology, as well as to make Ibis fully open source. Results: We introduce here freeIbis, which offers significant improvements in sequence accuracy owing to the use of a novel multiclass support vector machine (SVM) algorithm. Sequence quality scores are now calibrated based on empirically observed scores, thus providing a high correlation to their respective error rates. These improvements result in downstream advantages including improved genotyping accuracy. Availability and implementation: FreeIbis is freely available for use under the GPL (http://bioinf.eva.mpg.de/freeibis/). It requires a Python interpreter and a C++ compiler. Tailored versions of LIBOCAS and LIBLINEAR are distributed along with the package. Contact: [email protected] Supplementary information: Supplementary data are available at Bioinformatics online

Leather properties as a function of cattle breed

Content: Since hundreds of years, tanners share the opinion that hides from different cattle breeds lead to varying leather qualities. Especially European hides from the alpine region (e. g. Simmentaler or brown origin) are preferred by tanners. These leathers feature a higher thickness, a maximum utilisation induced by a minor thickness difference over the whole area and a lower tensile strength in contrast to leathers from other breeds. However, are these alpine hides better because of their breed affiliation or because they are kept in special regional conditions? It is known that, besides the breed, also other factors can influence the rawhide and leather quality like age, gender, nutrition and climate conditions. In addition, present dairy and beef cattle are high-performance cattle by breeding, which leads to more crossbreeds than 100 years ago. Our intention was to find out, whether leather quality nowadays is still a function of breed or not. For that purpose, 40 rawhides from four different cattle breeds (Angus, Charolais, Simmentaler, Limousin) were collected from the Saxon region. From each breed, five male and five female rawhides were collected. The age of each individual was restricted to two years. All 40 rawhides were tanned with the same technology for furniture leather. Leather quality was characterized by determining chemical and physical parameters. Chemical parameters included collagen content, fat content and ignition lost (DIN 181218). The physical parameters were tensile strength (DIN EN ISO 3376), elongation at break (DIN EN ISO 3376) and stitch tear resistance (DIN EN ISO 23910). The analyses revealed that the chemical parameters were identical for all examined breeds. For this reason, the chemical composition of a cattle skin is irrespective of breed origin. The tensile strength of the leathers showed only a small significant difference between Angus and Limousin (p= 0.05). Leathers from Limousin hides showed significantly different elongations at break compared to Angus, Charolais and Simmentaler. The stitch tear resistance varied in nearly all breeds. Significant differences were detected between all breeds except between Angus and Charolais as well as Angus and Simmentaler. Plotting the measured physical values against gender or age of the individuals showed no correlation. In summary, only minor differences between the cattle breeds were found. But this tendency must be confirmed by a larger quantity of test individuals. For this purpose, an analysis is planed with 100 individuals from different breeds and crossbreeds. Take-Away: Many tanners share the opinion that hides from different attle breeds lead to varying leather qualities. We found only minor differences of the physical parameters between the cattle breeds with a random sample of 10 individuals per breed. To confirm this tendency, an analysis is planed with 100 individuals from different breeds and crossbreeds

Supersymmetric M3-branes and G_2 Manifolds

We obtain a generalisation of the original complete Ricci-flat metric of G_2 holonomy on R^4\times S^3 to a family with a non-trivial parameter \lambda. For generic \lambda the solution is singular, but it is regular when \lambda={-1,0,+1}. The case \lambda=0 corresponds to the original G_2 metric, and \lambda ={-1,1} are related to this by an S_3 automorphism of the SU(2)^3 isometry group that acts on the S^3\times S^3 principal orbits. We then construct explicit supersymmetric M3-brane solutions in D=11 supergravity, where the transverse space is a deformation of this class of G_2 metrics. These are solutions of a system of first-order differential equations coming from a superpotential. We also find M3-branes in the deformed backgrounds of new G_2-holonomy metrics that include one found by A. Brandhuber, J. Gomis, S. Gubser and S. Gukov, and show that they also are supersymmetric.Comment: Latex, 29 pages. This corrects a previous version in which it was claimed that the M3-brane solutions were pseudo-supersymmetric rather than supersymmetri

On The Inflaton Potential From Antibranes in Warped Throats

We compute the force between a stack of smeared antibranes at the bottom of a warped throat and a stack of smeared branes at some distance up the throat, both for anti-D3 branes and for anti-M2 branes. We perform this calculation in two ways: first, by treating the antibranes as probes in the background sourced by the branes and second, by treating the branes as probes in the candidate background sourced by the antibranes. These two very different calculations yield exactly the same expression for the force, for all values of the brane-antibrane separation. This indicates that the force between a brane and an antibrane is not screened in backgrounds where there is positive charge dissolved in flux, and gives a way to precisely compute the inflaton potential in certain string cosmology scenarios.Comment: 9 page