Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples

Background: Escherichia coli O157:H7 infection causes hemorrhagic colitis and is diagnosed based on symptoms such as cramps, stomach pain, and watery diarrhea. Shiga-like toxins (Verotoxin) produced by Escherichia coli O157:H7 damages endothelial cells of both kidney and brain, causing renal dysfunction and neurological problems. Methods: The present study focuses on identifying the prevalence of Verotoxin- producing Escherichia coli O157:H7 among diarrheal inpatients at Erode Government Hospital, India, and its antibiogram. Further, the Verotoxins were characterized by using SDS-PAGE analysis. A total of 123 diarrheal stool samples were collected, and 37 of them (30.08% of the total samples) were found to have the presence of E. coli. The organisms were identified based on their colony morphology on various media, cell morphology, and biochemical tests. The Shiga-like toxin production was identified by non-fermentation of sorbitol on SMAC agar plates. Confirmation of Shiga-like toxin was performed using agglutination assay.Results: In total, 12 isolates showed agglutination and these isolates were confirmed to be E. coli O157:H7. The molecular weight of the Verotoxin was found to be between 20 and 29 kD. The antibiogram profile of the four isolated strains against 10 standard antibiotics was determined.Conclusion: The results of this study show the occurrence of drug resistance on hemorrhagic colitis causing E. coli O157:H7. Keywords: E. coli, Verotoxin, diarrhea, hemorrhagic coliti

Catalytic non-thermal plasma reactor for decomposition of dilute chlorobenzene

Oxidative decomposition of low concentrations of chlorobenzene (CB) in air was carried out in a NTP reactor. Typical results indicated the better performance on addition of metal oxide catalysts in plasma zone. It may be concluded that catalytic plasma approach has promise, especially for the removal of low concentraions of CB, where conventional techniques are not energetically feasible. Among the metal oxides studied, AgOx/MnOx showed the better performance than MnOx and CoOx. During the removal of 50 ppm of CB, AgOx/MnOx under humid conditions showed 100% selectivity to total oxidadtion at 260 J L-1, which may be assigned due to the formation of hydroxyl radical and/or due to in situ ozone decomposition on the catalyst surface that may lead to the formation of a powerful oxidant atomic oxygen. Oxidative decomposition of low concentrations of chlorobenzene in air was carried out in a NTP reactor. Results indicated the improved performance on addition of catalysts in plasma zone. It has been observed that the integration of metal oxides, promoted the total oxidation, whose activity was further enhanced on addition of water vapor. During the abatement of 50 ppm of CB, selectivity to CO2 was 100% at 260 J L-1 with AgOx/MnOx/SMF catalytic electrode

人の集団に関する新たな評価方法の考察

九州大学応用力学研究所研究集会報告 No.20ME-S7 「非線形波動の数理と物理」RIAM Symposium No.20ME-S7 Mathematics and Physics in Nonlinear waves本研究では、金融工学の手法を用いて人の混雑を評価する理論の構築を試みた。我々は、人の混雑を表すダイナミクスは確率項を含むLogistic 方程式に従うと仮定し、この動きを正味現在価値やリアルオプション法によって評価した。また、金融工学では、割引率を用いることによってタイミングによる価値の違いが表現されるが今回のモデルでは、割引率を人の効用を割り引くために導入し、実データに基づく推定・考察もあわせて行った

Catalytic non-thermal plasma reactor for the decomposition of a mixture of volatile organic compounds

The decomposition of mixture of selected volatile organic compounds (VOCs) has been studied in a catalytic non-thermal plasma dielectric barrier discharge reactor. The VOCs mixture consisting n-hexane, cyclo-hexane and p-xylene was chosen for the present study. The decomposition characteristics of mixture of VOCs by the DBD reactor with inner electrode modified with metal oxides of Mn and Co was studied. The results indicated that the order of the removal efficiency of VOCs followed as p-xylene > cyclo-hexane > n-hexane. Among the catalytic study, MnOx/SMF (manganese oxide on sintered metal fibres electrode) shows better performance, probably due to the formation of active oxygen species by in situ decomposition of ozone on the catalyst surface. Water vapour further enhanced the performance due to the in situ formation of OH radicals. [Figure not available: see fulltext.

Risk factors of musculoskeletal disorders (MSD) among palm oil mill workers

The palm oil industry is the fourth largest contributor to the Malaysian Gross National Income (GNI) but the importance of this industry to the nation’s economy does not preclude them from the risk of being affected by the occurrence of musculoskeletal disorders (MSD) among the workers in palm oil mills. Previous studies carried out have not properly explained the exact reasons for the occurrence of musculoskeletal disorders (MSD) among the palm oil mill workers. Thus, this study aim to determine the association between risk factors and MSD among palm oil mill workers. A total of 120 male workers were consecutively enrolled in this crosssectional study. Information on sociodemographic, working, lifestyle, health and injury factors were collected via questionnaire and face-to-face interviews. A high lifetime prevalence of MSD (71.7 percent) among palm oil mill workers was found. However, the one-year prevalence of MSD among the same population was only 50.8 percent. Logistic regression analysis adjusted for age, Body Mass Index (BMI) and smoking revealed that the risk factors: history of previous injury [2.35, 95 percent CI 1.12-4.93], Osteoarthritis [3.9, 95 percent CI 1.568-9.708] and perception of exertion [8.09, 95 percent CI 1.358-48.170] was found to be significantly associated with MSD symptoms reported in the past 12 months. As a conclusion, exposure to the combination of these risk factors may lead to an increased risk of developing MSD among palm oil mill workers

BOILED WATER TEMPERATURE MEASUREMENT SYSTEM USING PIC MICROCONTROLLER

ABSTRACT: The measurement system for temperature of boiled water is a critical task in industry. In this paper we designed and implemented a PIC micro controller based boiled water temperature measurement system using PIC 18F452 and national semiconductors LM35 temperature sensor. The designing system is used to measure the tank I boiled water temperature value. If the temperature value reaches the set value high temperature relay board becomes ON to control the solenoid valve. The high temperature of the boiled water which flows through the metal pipe I is lowered by spraying the cool water using control valves. The valves are controlled by manually depends upon the requirement of temperature. Thus the output is obtained by reducing high temperature. Low temperature water is collected in tank III. Here, after comparing the reduced temperature value water with programming of temperature set value, the collected cooling water is ready for supplying to various purposes in the industry plants

Computer Vision and Image Understanding xxx

Abstract 13 This paper presents a panoramic virtual stereo vision approach to the problem of detecting 14 and localizing multiple moving objects (e.g., humans) in an indoor scene. Two panoramic 15 cameras, residing on different mobile platforms, compose a virtual stereo sensor with a flexible 16 baseline. A novel ''mutual calibration'' algorithm is proposed, where panoramic cameras on 17 two cooperative moving platforms are dynamically calibrated by looking at each other. A de-18 tailed numerical analysis of the error characteristics of the panoramic virtual stereo vision 19 (mutual calibration error, stereo matching error, and triangulation error) is given to derive 20 rules for optimal view planning. Experimental results are discussed for detecting and localizing 21 multiple humans in motion using two cooperative robot platforms. 2

Chikungunya: A Potentially Emerging Epidemic?

Chikungunya virus is a mosquito-borne emerging pathogen that has a major health impact in humans and causes fever disease, headache, rash, nausea, vomiting, myalgia, and arthralgia. Indigenous to tropical Africa, recent large outbreaks have been reported in parts of South East Asia and several of its neighboring islands in 2005–07 and in Europe in 2007. Furthermore, positive cases have been confirmed in the United States in travelers returning from known outbreak areas. Currently, there is no vaccine or antiviral treatment. With the threat of an emerging global pandemic, the peculiar problems associated with the more immediate and seasonal epidemics warrant the development of an effective vaccine. In this review, we summarize the evidence supporting these concepts