In situ enrichment of ocean crust microbes on igneous minerals and glasses using an osmotic flow-through device

Author Posting. © American Geophysical Union, 2011. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry Geophysics Geosystems 12 (2011): Q06007, doi:10.1029/2010GC003424.The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.The subseafloor flow cell enrichment chambers were funded by a small grant from the Ocean Drilling Program. This work was also funded by NASA grant NNX08AO22G, NSF OCE 0727119 to C.G.W., NSF OCE 0452333 to S.M.S., and OCE‐0550713 and OCE‐0727952 to A.T.F., PSU, and OSU

Seismogenic zone structure beneath the Nicoya Peninsula, Costa Rica, from three-dimensional local earthquake P- and S-wave tomography

The subduction plate interface along the Nicoya Peninsula, Costa Rica, generates damaging large (Mw > 7.5) earthquakes. We present hypocenters and 3-D seismic velocity models (VP and VP/VS) calculated using simultaneous inversion of P- and S-wave arrival time data recorded from small magnitude, local earthquakes to elucidate seismogenic zone structure. In this region, interseismic cycle microseismicity does not uniquely define the potential rupture extent of large earthquakes. Plate interface microseismicity extends from 12 to 26 and from 17 to 28 km below sea level beneath the southern and northern Nicoya Peninsula, respectively. Microseismicity offset across the plate suture of East Pacific Rise-derived and Cocos-Nazca Spreading Center-derived oceanic lithosphere is ∼5 km, revising earlier estimates suggesting ∼10 km of offset. Interplate seismicity begins downdip of increased locking along the plate interface imaged using GPS and a region of low VP along the plate interface. The downdip edge of plate interface microseismicity occurs updip of the oceanic slab and continental Moho intersection, possibly due to the onset of ductile behaviour. Slow forearc mantle wedge P-wave velocities suggest 20–30 per cent serpentinization across the Nicoya Peninsula region while calculated VP/VS values suggest 0–10 per cent serpentinization. Interpretation of VP/VS resolution at depth is complicated however due to ray path distribution. We posit that the forearc mantle wedge is regionally serpentinized but may still be able to sustain rupture during the largest seismogenic zone earthquakes

Sustainability and dynamics of outcrop-to-outcrop hydrothermal circulation

Most seafloor hydrothermal circulation occurs far from the magmatic influence of mid-ocean ridges, driving large flows of water, heat and solutes through volcanic rock outcrops on ridge flanks. Here we create three-dimensional simulations of ridge–flank hydrothermal circulation, flowing between and through seamounts, to determine what controls hydrogeological sustainability, flow rate and preferred flow direction in these systems. We find that sustaining flow between outcrops that penetrate less-permeable sediment depends on a contrast in transmittance (the product of outcrop permeability and the area of outcrop exposure) between recharging and discharging sites, with discharge favoured through less-transmissive outcrops. Many simulations include local discharge through outcrops at the recharge end of an outcrop-to-outcrop system. Both of these characteristics are observed in the field. In addition, smaller discharging outcrops sustain higher flow rates than larger outcrops, which may help to explain how so much lithospheric heat is extracted globally by this process