298 research outputs found
Accreditation by Design: Construction of an Instrument to Measure Teacher Candidates’ Perceptions of Preparedness to Meet InTASC Standards
The InTASC Candidate Self-Perception Instrument (ICSPI) is an innovative, high-quality educational measurement tool designed to support the assessment and accreditation efforts of a wide variety of educator preparation programs (EPP). The procedures used for the creation and refining of items for the ICSPI are presented, including empirical estimates of reliability from a trial implementation across three semesters. Evidence of instrument validity is discussed, while observed reliability estimates were found to be above acceptable levels for all subgroups. The ICSPI can be used to evaluate EPPs’ course offerings and requirements, align course outcomes with educational standards, provide data to advise programs of relative strengths and weaknesses, and improve teacher preparation
Cascading effects of canopy mortality drive long-term changes in understorey diversity in temperate old-growth forests of Europe
Questions: We investigated the influence of protracted mortality of a dominant canopy tree (Abies alba) on long-term understorey dynamics. We ask (a) how tree regeneration and understorey species diversity and composition changed over 32 years; and (b) whether the observed changes were mainly driven by mortality of A. alba.
Location: Three old-growth forest reserves dominated by A. alba and Fagus sylvatica in the Dinaric Mountains of Slovenia.
Method: Tree layer and understorey regeneration and herbs were surveyed in 147 plots across the three forest reserves in 1983 and 2015. Soils were also sampled in 2015. The study period coincides with a protracted period of increased A. alba mortality in the canopy layer associated with anthropogenic emissions.
Results: Between 1983 and 2015, the decline in canopy layer A. alba caused a recruitment pulse of F. sylvatica regeneration to the subcanopy tree layer across the three reserves. These changes were accompanied by a significant decline in plot level herb species richness. A model-based analysis of beta-diversity revealed significant community convergence during the study period, mainly caused by the loss of rare species. Ellenberg values indicate that these changes were mainly driven by loss of understorey light, while an increase in soil pH may have played a role also.
Conclusions: This observational study suggests that the long-term decline of A. alba resulted in a cascade of processes - widespread F. sylvatica recruitment that impeded penetration of light to the forest floor, and possibly a change in soil conditions due to the decline of coniferous litter. These changes caused a significant loss of herb diversity and homogenization of the understorey community across the three sites. This study sheds light on the potential cascading consequences triggered by episodes of increased tree mortality resulting from global-change-type drivers
Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines
ABCC1 and ABCC4 utilize energy from ATP hydrolysis to transport many different molecules, including drugs, out of the cell and, as such, have been implicated in causing drug resistance. However recently, because of their ability to transport signaling molecules and inflammatory mediators, it has been proposed that ABCC1 and ABCC4 may play a role in the hallmarks of cancer development and progression, independent of their drug efflux capabilities. Breast cancer is the most common cancer affecting women. In this study, the aim was to investigate whether ABCC1 or ABCC4 play a role in the proliferation or migration of breast cancer cell lines MCF-7 (luminal-type, receptor-positive) and MDA-MB-231 (basal-type, triple-negative). The effects of small molecule inhibitors or siRNA-mediated knockdown of ABCC1 or ABCCC4 were measured. Colony formation assays were used to assess the clonogenic capacity, MTT assays to measure the proliferation, and scratch assays and Transwell assays to monitor the cellular migration. The results showed a role for ABCC1 in cellular proliferation, whilst ABCC4 appeared to be more important for cellular migration. ELISA studies implicated cAMP and/or sphingosine-1-phosphate efflux in the mechanism by which these transporters mediate their effects. However, this needs to be investigated further, as it is key to understand the mechanisms before they can be considered as targets for treatment
The synthesis of recombinant membrane proteins in yeast for structural studies
Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that are available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies
First detection of NH3 (1,0 - 0,0) from a low mass cloud core: On the low ammonia abundance of the rho Oph A core
Odin has successfully observed the molecular core rho Oph A in the 572.5 GHz
rotational ground state line of ammonia, NH3 (J,K = 1,0 - 0,0). The
interpretation of this result makes use of complementary molecular line data
obtained from the ground (C17O and CH3OH) as part of the Odin preparatory work.
Comparison of these observations with theoretical model calculations of line
excitation and transfer yields a quite ordinary abundance of methanol, X(CH3OH)
= 3e-9. Unless NH3 is not entirely segregated from C17O and CH3OH, ammonia is
found to be significantly underabundant with respect to typical dense core
values, viz. X(NH3) = 8e-10.Comment: 4 pages, 2 figures, 2 tables, to appear in Astron. Astrophys. Letter
Gastric cancer and Helicobacter pylori: a combined analysis of 12 case control studies nested within prospective cohorts
BACKGROUND: The magnitude of the association
between Helicobacter pylori and
incidence of gastric cancer is unclear. H
pylori infection and the circulating antibody
response can be lost with development
of cancer; thus retrospective studies
are subject to bias resulting from classifi-
cation of cases as H pylori negative when
they were infected in the past.
AIMS: To combine data from all case control
studies nested within prospective
cohorts to assess more reliably the relative
risk of gastric cancer associated with H
pylori infection.To investigate variation in
relative risk by age, sex, cancer type and
subsite, and interval between blood sampling
and cancer diagnosis.
METHODS: Studies were eligible if blood
samples for H pylori serology were collected
before diagnosis of gastric cancer in
cases. Identified published studies and two
unpublished studies were included. Individual
subject data were obtained for
each. Matched odds ratios (ORs) and 95%
confidence intervals (95% CI) were calculated
for the association between H pylori
and gastric cancer.
RESULTS: Twelve studies with 1228 gastric
cancer cases were considered. The association
with H pylori was restricted to noncardia
cancers (OR 3.0; 95% CI 2.3–3.8)
and was stronger when blood samples for
H pylori serology were collected 10+ years
before cancer diagnosis (5.9; 3.4–10.3). H
pylori infection was not associated with an
altered overall risk of cardia cancer (1.0;
0.7–1.4).
CONCLUSIONS: These results suggest that
5.9 is the best estimate of the relative risk
of non-cardia cancer associated with H
pylori infection and that H pylori does not
increase the risk of cardia cancer. They
also support the idea that when H pylori
status is assessed close to cancer diagnosis,
the magnitude of the non-cardia
association may be underestimated
Submillimeter Emission from Water in the W3 Region
We have mapped the submillimeter emission from the 1(10)-1(01) transition of
ortho-water in the W3 star-forming region. A 5'x5' map of the W3 IRS4 and W3
IRS5 region reveals strong water lines at half the positions in the map. The
relative strength of the Odin lines compared to previous observations by SWAS
suggests that we are seeing water emission from an extended region. Across much
of the map the lines are double-peaked, with an absorption feature at -39 km/s;
however, some positions in the map show a single strong line at -43 km/s. We
interpret the double-peaked lines as arising from optically thick,
self-absorbed water emission near the W3 IRS5, while the narrower blue-shifted
lines originate in emission near W3 IRS4. In this model, the unusual appearance
of the spectral lines across the map results from a coincidental agreement in
velocity between the emission near W3 IRS4 and the blue peak of the more
complex lines near W3 IRS5. The strength of the water lines near W3 IRS4
suggests we may be seeing water emission enhanced in a photon-dominated region.Comment: Accepted to A&A Letters as part of the special Odin issue; 4 page
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