22,034 research outputs found
The impact of columnar file formats on SQLâonâhadoop engine performance: A study on ORC and Parquet
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Peroxisomes in intestinal and gallbladder epithelial cells of the stickleback, Gasterosteus aculeatus L. (Teleostei)
The occurrence of microbodies in the epithelial cells of the intestine and gallbladder of the stickleback, Gasterosteus aculeatus L., is described. In the intestine the organelles are predominantly located in the apical and perinuclear zone of the cells and may contain small crystalline cores. In gallbladder epithelial cells the microbodies are distributed randomly. The latter organdies are characterized by the presence of large crystalloids. Cytochemical and biochemical experiments show that catalase and D-amino acid oxidase are main matrix components of the microbodies in both the intestinal and gallbladder epithelia. These organelles therefore are considered peroxisomes. In addition, in intestinal mucosa but not in gallbladder epithelium a low activity of palmitoyl CoA oxidase was detected biochemically. Urate oxidase and L-Îą hydroxy acid oxidase activities could not be demonstrated.
On the unification of dwarf and giant elliptical galaxies
The near orthogonal distributions of dwarf elliptical (dE) and giant
elliptical (E) galaxies in the mu_e-Mag and mu_e-log(R_e) diagrams have been
interpreted as evidence for two distinct galaxy formation processes. However,
continuous, linear relationships across the alleged dE/E boundary at M_B = -18
mag - such as those between central surface brightness (mu_0) and (i) galaxy
magnitude and (ii) light-profile shape (n) - suggest a similar, governing
formation mechanism. Here we explain how these latter two linear trends
necessitate a different behavior for dE and E galaxies, exactly as observed, in
diagrams involving mu_e (and also _e). A natural consequence is that the
distribution of dEs and Es in Fundamental Plane type analyses that use the
associated intensity I_e, or _e, are expected to appear different. Together
with other linear trends across the alleged dE/E boundary, such as those
between luminosity and color, metallicity, and velocity dispersion, it appears
that the dEs form a continuous extension to the E galaxies. The presence of
partially depleted cores in luminous (M_B < -20.5 mag) Es does however signify
the action of a different physical process at the centers (< ~300 pc) of these
galaxies.Comment: 5 pages from the proceedings of the 2004 conference "Penetrating bars
through masks of cosmic dust: the Hubble tuning fork strikes a new note".
Edited by D. L. Block, I. Puerari, K. C. Freeman, R. Groess, and E. K. Bloc
Heterologously-expressed and Liposome-reconstituted Human Transient Receptor Potential Melastatin 4 Channel (TRPM4) is a Functional Tetramer
Mutation, irregular expression and sustained activation of the Transient Receptor Potential Channel, type Melastatin 4 (TRPM4), have been linked to various cardiovascular diseases. However, much remains unknown about the structure of this important ion channel. Here, we have purified a heterologously expressed TRPM4-eGFP fusion protein and investigated the oligomeric state of TRPM4-eGFP in detergent micelles using crosslinking, native gel electrophoresis, multi-angle laser light scattering and electron microscopy. Our data indicate that TRPM4 is tetrameric, like other TRP channels studied to date. Furthermore, the functionality of liposome reconstituted TRPM4-eGFP was examined using electrophysiology. Single-channel recordings from TRPM4-eGFP proteoliposomes showed inhibition of the channel using Flufenamic acid, a well-established inhibitor of TRPM4, suggesting that the channels are functional upon reconstitution. Our characterisation of the oligomeric structure of TRPM4 and the ability to reconstitute functional channels in liposomes should facilitate future studies into the structure, function and pharmacology of this therapeutically relevant channel
Shifting Attention From Theory to Practice in Philosophy of Biology
Traditional approaches in philosophy of biology focus attention on biological concepts, explanations, and theories, on evidential support and inter-theoretical relations. Newer approaches shift attention from concepts to conceptual practices, from theories to practices of theorizing, and from theoretical reduction to reductive retooling. In this article, I describe the shift from theory-focused to practice-centered philosophy of science and explain how it is leading philosophers to abandon fundamentalist assumptions associated with traditional approaches in philosophy of science and to embrace scientific pluralism. This article comes in three parts, each illustrating the shift from theory-focused to practice-centered epistemology. The first illustration shows how shifting philosophical attention to conceptual practice reveals how molecular biologists succeed in identifying coherent causal strands within systems of bewildering complexity. The second illustration suggests that analyzing how a multiplicity of alternative models function in practice provides an illuminating approach for understanding the nature of theoretical knowledge in evolutionary biology. The third illustration demonstrates how framing reductionism in terms of the reductive retooling of practice offers an informative perspective for understanding why putting DNA at the center of biological research has been incredibly productive throughout much of biology. Each illustration begins by describing how traditional theory-focused philosophical approaches are laden with fundamentalist assumptions and then proceeds to show that shifting attention to practice undermines these assumptions and motivates a philosophy of scientific pluralism
Facile one-pot synthesis of amoxicillin-coated gold nanoparticles and their antimicrobial activity
Nanomaterials have been the object of intense study due to promising applications in a number of different disciplines. In particular, medicine and biology have seen the potential of these novel materials with their nanoscale properties for use in diverse areas such as imaging, sensing and drug vectorisation. Gold nanoparticles (GNPs) are considered a very useful platform to create a valid and efficient drug delivery/carrier system due to their facile and well-studied synthesis, easy surface functionalization and biocompatibility. In the present study, stable antibiotic conjugated GNPs were synthesised by a one-step reaction using a poorly water soluble antibiotic, amoxicillin. Amoxicillin, a member of the penicillin family, reduces the chloroauric acid to form nanoparticles and at the same time coats them to afford the functionalised nanomaterial. A range of techniques including UV-vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and thermogravimetric analysis (TGA) were used to ascertain the gold/drug molar ratio and the optimum temperature for synthesis of uniform monodisperse particles in the ca. 30-40 nm size range. Amoxicillin-conjugated gold showed an enhancement of antibacterial activity against Escherichia coli compared to the antibiotic alone
Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity
Signals recorded from neurons with extracellular planar sensors have a wide
range of waveforms and amplitudes. This variety is a result of different
physical conditions affecting the ion currents through a cellular membrane. The
transmembrane currents are often considered by macroscopic membrane models as
essentially a homogeneous process. However, this assumption is doubtful, since
ions move through ion channels, which are scattered within the membrane.
Accounting for this fact, the present work proposes a theoretical model of
heterogeneous membrane conductivity. The model is based on the hypothesis that
both potential and charge are distributed inhomogeneously on the membrane
surface, concentrated near channel pores, as the direct consequence of the
inhomogeneous transmembrane current. A system of continuity equations having
non-stationary and quasi-stationary forms expresses this fact mathematically.
The present work performs mathematical analysis of the proposed equations,
following by the synthesis of the equivalent electric element of a
heterogeneous membrane current. This element is further used to construct a
model of the cell-surface electric junction in a form of the equivalent
electrical circuit. After that a study of how the heterogeneous membrane
conductivity affects parameters of the extracellular electrical signal is
performed. As the result it was found that variation of the passive
characteristics of the cell-surface junction, conductivity of the cleft and the
cleft height, could lead to different shapes of the extracellular signals
Risk of prostate cancer after isolated high-grade prostatic intraepithelial neoplasia (HGPIN) detected on extended core needle biopsy : a UK hospital experience.
Background High-grade prostatic intraepithelial neoplasia (HGPIN) is a precursor lesion to prostate cancer (CaP). UK-based studies examining the occurrence of isolated HGPIN and subsequent risk of CaP are lacking. Our aim was to assess the occurrence of HGPIN in a regional UK population and to determine whether in a retrievable cohort of such patients that had repeat extended core biopsies, there was an elevated risk of CaP. Methods A retrospective analysis of the pathology database was conducted at our institution (Lancashire Teaching Hospitals NHS Foundation Trust) for prostate biopsies recorded between January 2001 and December 2005 (all extended core biopsies). Those patients with isolated HGPIN on 1st set of biopsies were identified and, their clinical characteristics and pathological findings from subsequent biopsies (if any) were determined. The risk of CaP on subsequent biopsies based on presenting baseline PSA was stratified. Results Of 2,192 biopsied patients, there were 88 cases of isolated HGPIN of which 67 patients underwent one or more repeat biopsies. In this repeat-biopsy group, 28 CaP diagnoses were made. Age at first biopsy (P 20 ng/ml â 87.5%. Conclusion Based on our results, we recommend delaying the 1st repeat biopsy at low PSA range but to have a shorter interval to repeat biopsies at intermediate and higher PSA ranges
Organization of Multinational Activities and Ownership Structure
We develop a model in which multinational investors decide about the modes of organization, the locations of production, and the markets to be served. Foreign investments are driven by market-seeking and cost-reducing motives. We further assume that investors face costs of control that vary among sectors and increase in distance. The results show that (i) production intensive sectors are more likely to operate a foreign business independent of the investment motive, (ii) that distance may have a non-monotonous effect on the likelihood of horizontal investments, and (iii) that globalization, if understood as reducing distance, leads to more integration
Shaping the Phase of a Single Photon
While the phase of a coherent light field can be precisely known, the phase
of the individual photons that create this field, considered individually,
cannot. Phase changes within single-photon wave packets, however, have
observable effects. In fact, actively controlling the phase of individual
photons has been identified as a powerful resource for quantum communication
protocols. Here we demonstrate the arbitrary phase control of a single photon.
The phase modulation is applied without affecting the photon's amplitude
profile and is verified via a two-photon quantum interference measurement,
which can result in the fermionic spatial behaviour of photon pairs. Combined
with previously demonstrated control of a single photon's amplitude, frequency,
and polarisation, the fully deterministic phase shaping presented here allows
for the complete control of single-photon wave packets.Comment: 4 pages, 4 figure
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