Improving the accuracy of 1D SNMR surveys using the multi-central-loop configuration

Temeljna svrha i cilj ovoga rada bilo je ispitati koliko su potrošači skloni dijeljenju svojih turističkih iskustva s drugima te putem kojih medija. Osim navedenog, drugi cilj provedenog istraživanja bilo je utvrditi koliko su potrošačima važna iskustva i komentari drugih posjetitelja u procesu donošenja odluke o kupnji. Istraživanje je provedeno metodom ispitivanja, a kao instrument korišten je anketni upitnik sastavljen od 22 pitanja. Utvrđivanjem problema istraživanja, postavljene su tri hipoteze. Od tri hipoteze, u potpunosti je dokazana samo prva koja pretpostavlja da su potrošačima tuđa iskustva i komentari od velike važnosti kod planiranja i odabira putovanja. Druga hipoteza je djelomično potvrđena, tj. potvrđeno je da su potrošači skloni dijeliti svoja iskustva s drugima u situaciji kada su jako zadovoljni dok s druge strane nije potvrđeno kako su potrošači skloni dijeliti svoja iskustva u situaciji kada su nezadovoljni uslugom ili proizvodom. Na kraju, potvrđena je i treća hipoteza koja pretpostavlja kako su potrošači skloni dijeljenju vlastitog turističkog iskustva putem više društvenih medija, iako je utvrđeno kako najveći broj ispitanika ne dijeli svoja turistička iskustva. Istraživano je i mišljenje ispitanika o turističkoj destinaciji iz snova, a iznenađujuće, najveći broj ispitanika je navelo hrvatske destinacije kao svoje destinacije iz snova kao i one koje su im dosada pružile najnezaboravnije turističko iskustvo. Potrebno je provesti detaljnija istraživanja kako bi se detaljnije istražilo novije društvene medije koji su dostupni potrošačima za dijeljenje svog iskustva

Improving the accuracy of 1D SNMR surveys using the multi-central-loop configuration

A multi-central loop configuration has been studied through forward and inverse modelling of synthetics and real data. This set-up takes advantage of the multichannel features of the NMR device and consists of using several (2 to 3) additional receiver loops displayed concentrically with the main transmitter/receiver loop, which all record the NMR signal simultaneously within a single acquisition. If the loop diameters are chosen appropriately, the kernel sensitivity distributions for each receiver loop can show complementary features. Inverting simultaneously the data sets obtained through each different receiver loop can then enhance the accuracy of the final model. To do so, a 1D QT inversion scheme in the frequency domain dedicated to the inversion of multiple data sets is being used. One challenging feature is to adapt the regularization of the inverse process so as to handle correctly the noise originating from different data sets. The efficiency of this multi-central loop acquisition set-up and procedure is being assessed through the forward and inverse modelling of several scenarios implying varying aquifer characteristics. Finally a field case is being presented that was conducted on a low noise level site located in Germany, where conditions were favourable to the implementation and testing of circular multi-central loop configurations.We also introduce a new method for determining NMR parameters, named the prediction-focused-approach (PFA), that is based on statistical analysis of a large number of simple models. We observe, using synthetic examples, that the effciency of the method benefits from the use of the multi-central-loop configurations

Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31

A purification procedure for a new kind of extradiol dioxygenase, termed chlorocatechol 2,3-dioxygenase, that converts 3-chlorocatechol productively was developed. Structural and kinetic properties of the enzyme, which is part of the degradative pathway used for growth of Pseudomonas putida GJ31 with chlorobenzene, were investigated. The enzyme has a subunit molecular mass of 33.4 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Estimation of the native Mr value under nondenaturating conditions by gel filtration gave a molecular mass of 135 ± 10 kDa, indicating a homotetrameric enzyme structure (4 × 33.4 kDa). The pI of the enzyme was estimated to be 7.1 ± 0.1. The N-terminal amino acid sequence (43 residues) of the enzyme was determined and exhibits 70 to 42% identity with other extradiol dioxygenases. Fe(II) seems to be a cofactor of the enzyme, as it is for other catechol 2,3-dioxygenases. In contrast to other extradiol dioxygenases, the enzyme exhibited great sensitivity to temperatures above 40°C. The reactivity of this enzyme toward various substituted catechols, especially 3-chlorocatechol, was different from that observed for other catechol 2,3-dioxygenases. Stoichiometric displacement of chloride occurred from 3-chlorocatechol, leading to the production of 2-hydroxymuconate.

Sialic Acid Mutarotation Is Catalyzed by the Escherichia coli β-Propeller Protein YjhT

The acquisition of host-derived sialic acid is an important virulence factor for some bacterial pathogens, but in vivo this sugar acid is sequestered in sialoconjugates as the {alpha}-anomer. In solution, however, sialic acid is present mainly as the β-anomer, formed by a slow spontaneous mutarotation. We studied the Escherichia coli protein YjhT as a member of a family of uncharacterized proteins present in many sialic acid-utilizing pathogens. This protein is able to accelerate the equilibration of the {alpha}- and β-anomers of the sialic acid N-acetylneuraminic acid, thus describing a novel sialic acid mutarotase activity. The structure of this periplasmic protein, solved to 1.5Å resolution, reveals a dimeric 6-bladed unclosed β-propeller, the first of a bacterial Kelch domain protein. Mutagenesis of conserved residues in YjhT demonstrated an important role for Glu-209 and Arg-215 in mutarotase activity. We also present data suggesting that the ability to utilize {alpha}-N-acetylneuraminic acid released from complex sialoconjugates in vivo provides a physiological advantage to bacteria containing YjhT

Influence of external flows on crystal growth: numerical investigation

We use a combined phase-field/lattice-Boltzmann scheme [D. Medvedev, K. Kassner, Phys. Rev. E {\bf 72}, 056703 (2005)] to simulate non-facetted crystal growth from an undercooled melt in external flows. Selected growth parameters are determined numerically. For growth patterns at moderate to high undercooling and relatively large anisotropy, the values of the tip radius and selection parameter plotted as a function of the Peclet number fall approximately on single curves. Hence, it may be argued that a parallel flow changes the selected tip radius and growth velocity solely by modifying (increasing) the Peclet number. This has interesting implications for the availability of current selection theories as predictors of growth characteristics under flow. At smaller anisotropy, a modification of the morphology diagram in the plane undercooling versus anisotropy is observed. The transition line from dendrites to doublons is shifted in favour of dendritic patterns, which become faster than doublons as the flow speed is increased, thus rendering the basin of attraction of dendritic structures larger. For small anisotropy and Prandtl number, we find oscillations of the tip velocity in the presence of flow. On increasing the fluid viscosity or decreasing the flow velocity, we observe a reduction in the amplitude of these oscillations.Comment: 10 pages, 7 figures, accepted for Physical Review E; size of some images had to be substantially reduced in comparison to original, resulting in low qualit

Ledoux-Convection in Protoneutron Stars --- a Clue to Supernova Nucleosynthesis?

Two-dimensional hydrodynamical simulations of the deleptonization of a newly formed neutron star were performed. Driven by negative lepton fraction and entropy gradients, convection starts near the neutrinosphere about 20-30 ms after core bounce, but moves deeper into the protoneutron star, and after about one second the whole protoneutron star is convective. The deleptonization of the star proceeds much faster than in the corresponding spherically symmetrical model because the lepton flux and the neutrino luminosities increase by up to a factor of two. The convection below the neutrinosphere raises the neutrinospheric temperatures and mean energies of the emitted neutrinos by 10-20%. This can have important implications for the supernova explosion mechanism and changes the detectable neutrino signal from the Kelvin-Helmholtz cooling of the protoneutron star. In particular, the enhanced electron neutrino flux relative to the electron antineutrino flux during the early post-bounce evolution might solve the overproduction problem of certain elements in the neutrino-heated ejecta in models of type-II supernova explosions.Comment: 17 pages, LaTeX, 8 postscript figures, uses epsf.sty. To appear in ApJ 473 (Letters), 1996 December 1

Estimates of the mean circulation in the deep (>2,000m) layer of the Eastern North Atlantic

A total of 131 current meter records of between 6 and 24 month duration are analysed to describe the deep flow field of the eastern North Atlantic from 19° to 54°N and from the Continental Slope to the Mid Atlantic Ridge. Mean flows are weak and may be statistically indeterminate in some records and locations, but appear to indicate cyclonic circulations around the Iberia and Porcupine abyssal plains with a generally southward flow along the Mid Atlantic ridge and a deep northward slope current (where measurements exist) along the eastern boundary. The deepest inflow to the north-eastern basin that has been identified to date takes place through the Discovery Gap of >4,700 m sill-depth at 37° 25′N 15° 45′W in the Azores-Portugal ridge. South of that ridge, observations are sparse and no systematic circulation is yet evident. These observations are discussed in relation to recent geostrophic estimates of the deep circulation

Structure prediction based on ab initio simulated annealing for boron nitride

Possible crystalline modifications of chemical compounds at low temperatures correspond to local minima of the energy landscape. Determining these minima via simulated annealing is one method for the prediction of crystal structures, where the number of atoms per unit cell is the only information used. It is demonstrated that this method can be applied to covalent systems, at the example of boron nitride, using ab initio energies in all stages of the optimization, i.e. both during the global search and the subsequent local optimization. Ten low lying structure candidates are presented, including both layered structures and 3d-network structures such as the wurtzite and zinc blende types, as well as a structure corresponding to the beta-BeO type