Uma proposta educativa a partir de práticas na construção de uma horta ecológica.

O trabalho compreende a descrição de uma proposta educativa a partir da construção de uma horta ecológica, sendo essa, uma ação ligada ao projeto de extensão “A Prática na Construção do Conhecimento Científico”. O desenvolvimento dessa proposta objetiva a construção do conhecimento cientifico e da cidadania por meio de atividades praticas cooperativas desenvolvidas durante a construção da horta. As atividades vêm sendo desenvolvidas semanalmente e conta com a participação de estudantes do ensino fundamental e médio do colégio estadual Monsenhor Guilherme, e vem apresentando resultados satisfatórios e motivadores ao longo dos encontros, pois os estudantes têm participado com assiduidade e engajamento, dando indicativos de que a proposta parece efetiva

Vivências em Laboratório: inserção de atividades experimentais em sala de aula

VII Seminário de Extensão Universitária da UNILA (SEUNI); VIII Encontro de Iniciação Científica e IV Encontro de Iniciação em Desenvolvimento Tecnológico e Inovação (EICTI 2019) e Seminário de Atividades Formativas da UNILA (SAFOR)O presente trabalho apresenta o papel da experimentação no ensino de Química na educação básica. O emprego de aulas experimentais é uma estratégia didática importante para o processo de construção do conhecimento científico, estimulando o caráter investigativo, a tomada de decisão e a aprendizagem colaborativa. A contextualização das atividades práticas propicia a correlação entre os conteúdos da Química e o cotidiano dos estudantes, contribuindo para a formação da cidadania. Além disso, tais atividades constituem fator para motivação dos estudantes para o ensino de QuímicaÁ PROEX/UNILA pela bolsa de extensão concedida e a direção e equipe pedagógica da escol

Stoichiometric genome-scale models for the chondroitin production in Escherichia coli

Chondroitin is a natural-occurring glycosaminoglycan with applications as a nutraceutical and pharmaceutical ingredient. It can be extracted from animal tissues, though chondroitin-like polysaccharides using microorganisms emerged as a safer and more sustainable alternative source. However, chondroitin yields using either natural or recombinant microorganisms are still far from meeting the increasing demand. In this work, stoichiometric models containing the heterologous pathway necessary for producing chondroitin in E. coli were constructed and investigated for mutant predictions that would potentially improve chondroitin yields. Four models of E. coli BL21 (BIGG ID: iECBD_1354, iECD_1391, iEC1356_Bl21DE3, iB21_1397) and one of E. coli K12 (BIGG ID: iJO1366), from which the other models were derived, were used to insert the heterologous pathway composed by two enzymatic steps catalyzed by UDP-Nacetylglucosamine 4-epimerase (UAE) and chondroitin synthase/polymerase (CHSY). The models were imported in Optflux, and the evolutionary optimization was then performed for gene deletion predictions using Strength Pareto Evolutionary Algorithm 2 (SPEA2) and the parsimonious Flux Balance Analysis (pFBA) as the simulation method. Chondroitin production was not predicted to improve by combining gene deletions, probably because the competing pathways that use the intermediates are critical for cell growth. However, gene over and underexpression search allowed to identify several targets. Most of the resulting solutions were composed by the overexpression of one of the genes responsible for the production of the heterologous pathway precursor (either glmU or glmM encoding glucosamine-1-phosphate Nacetyltransferase/UDP-N-acetylglucosamine diphosphorylase and phosphoglucosamine mutase, respectively) combined with the underexpression of one of the genes associated with cell wall recycling pathways (such as membrane-bound lytic transglycosylases mltA, mltB and mltC, or the anhydromuropeptide permease ampG), which contain reactions known to consume such precursors. The solutions herein obtained will be further validated in vivo by constructing the E. coli mutants predicted to improve chondroitin production.info:eu-repo/semantics/publishedVersio

O passado como imagem dialética: reflexões sobre as narrativas da italianidade em Santa Teresa/ES

Este artigo analisa construções simbólicas em torno da italianidade, expressas tanto nas práticas culturais de Santa Teresa/ES quanto na Lei nº 13.617/2018, que a reconhece como pioneira da imigração italiana no Brasil. Aproximando-se do conceito benjaminiano de imagem dialética, cuja formulação aborda relações do presente com o passado e aponta a linguagem como lugar de sua expressão, problematizamos a constituição de uma narrativa histórica que destaca apenas integrantes e valores da comunidade desejada. Compreendendo a cultura como um campo plural e dinâmico, tomamos a lei na acepção de mônada. O percurso metodológico escolhido nos instiga a refletir sobre a italianidade como uma construção social produtora de visões de mundo, sociabilidades e sensibilidades. Outrossim, fornece subsídios para realizarmos uma análise da cultura como espaço constituído por tensões, conflitos e negociações, ora ocorrendo de forma sutil, alegórica, consentida, ora de maneira impositiva e excludente

Neurocriptococose: diagnóstico por PCR

A detecção de Cryptococcus neoformans em líquor foi otimizada pela técnica de PCR. A amplificação foi realizada nas áreas ITS e 5,6S do RNA ribossomal (rRNA). Foram estudados 72 líquors obtidos de casos de pacientes com e sem AIDS. Os pacientes eram portadores de meningite criptocócica (n = 56) e meningite ocasionada por outros agentes (n = 16). Os resultados demonstraram que a técnica tem alta sensibilidade, superior a cultura (85,7%) e ao teste da tinta da china (76,8%). A técnica de PCR pode detectar 1 célula/mL de líquor e é altamente específica. A análise comparativa dos três métodos, tinta da china, cultura e PCR, demonstrou que o último é muito mais sensível e específico, podendo ser aplicável como importante recurso laboratorial no diagnóstico da neurocriptococose.Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by other agents (n = 16). The results demonstrated that PCR test had the highest sensitivity rates, superior to culture (85.7%) and to India ink test (76.8%). PCR was found to be sensitive in detecting 1 cell/mL and highly specific since it did not amplify other fungal DNA. The comparative analysis of the methods showed that PCR is more sensitive and specific and is applicable as an important laboratorial resource for neurocryptococcosis diagnosis

Alpha Cluster Structure in16O

The main purpose of the present work is the investigation of the α-cluster phenomenon in 16 O. The 12 C( 6 Li,d) 16 O reaction was measured at a bombarding energy of 25.5 MeV employing the Sao Paulo Pelletron-Enge-Spectrograph facility and the nuclear emulsion detection technique. Resonant states around 4α threshold were measured and an energy resolution of 15 keV allows to define states previously unresolved. The angular distributions of the absolute cross sections were determined in a range of 4-40 degree in the center of mass system. The upper limit for the resonance widths was obtained, indicating that the α cluster structure information in this region should be revised

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio