A multidimensional platform for the purification of non-coding RNA species

A renewed interest in non-coding RNA (ncRNA) has led to the discovery of novel RNA species and post-transcriptional ribonucleoside modifications, and an emerging appreciation for the role of ncRNA in RNA epigenetics. Although much can be learned by amplification-based analysis of ncRNA sequence and quantity, there is a significant need for direct analysis of RNA, which has led to numerous methods for purification of specific ncRNA molecules. However, no single method allows purification of the full range of cellular ncRNA species. To this end, we developed a multidimensional chromatographic platform to resolve, isolate and quantify all canonical ncRNAs in a single sample of cells or tissue, as well as novel ncRNA species. The applicability of the platform is demonstrated in analyses of ncRNA from bacteria, human cells and plasmodium-infected reticulocytes, as well as a viral RNA genome. Among the many potential applications of this platform are a system-level analysis of the dozens of modified ribonucleosides in ncRNA, characterization of novel long ncRNA species, enhanced detection of rare transcript variants and analysis of viral genomes.Singapore-MIT Alliance for Research and TechnologyNational Institute of Environmental Health Sciences (ES017010)National Institute of Environmental Health Sciences (ES002109

Mesa Directiva y Comisión de Gobierno en la Cámara de Diputados

A comparative study between Congress in Mexico and other Latin American countries is offered in this article. Starting with the manner in which the Government’s Commission functions within Congress, the writer tries to explain the way in which different countries conceive their parliamentary governability. To that effect, the author illustrates his analysis with examples of some Latin American congresses which lead him to propose three main types: (L) congresses where the Board of Chairmen takes over government tasks; ¿1 congresses where the Board forms part of the government Commission; and c) congresses where the Board and the Government Commission are entities totally independent from each other.En este artículo se realiza un estudio comparativo entre la Cámara de Diputados de México y las de diferentes países latinoamericanos. Iniciando con el propio funcionamiento de la Comisión de Gobierno de la Cámara, el autor quiere explicar la manera como diferentes países conciben su gobernabilidad parlamentaria. Para ello, el articulista ilustra su análisis con ejemplos de algunos congresos latinoamericanos de los cuales concluye proponiendo tres tipos fundamentales: a) congresos en los que la Mesa Directiva asume tareas de gobierno; b) congresos en los que la Mesa Directiva forma parte de la Comisión de Gobierno; y c) congresos en los que la Mesa Directiva y la Comisión de Gobierno son órganos independientes entre sí

Las elecciones mexicanas de 1995

RESUMEN: El artículo analiza las elecciones estatales y locales celebradas en México en 1995. El autor destaca cuatro factores presentes en estos comiciones. En primer lugar, se celebraron en un contexto de crisis económica. En segundo lugar, el PRI cosechó los peores resultados electorales de su historia. En tercer lugar, los procesos electorales (salvo quizá el de Yucatán) fueron limpios y transparentes. Por último, la competitividad del mercado electoral aumentó al concentrarse el voto opositor en una de las opciones, de la centro-derecha representada por el Partido Acción Nacional (PAN), y al perder presencia el partido opositor de centro-iexquierda Partido de la Revolución Democrática (PRD). Así, pues, tendió a fortalecerse el esquema bipartito PRI-PAN y a debilitarse el tripartidismo PRD-PRI-PAN. El autor concluye que 1995 fue un año de aceleración para el proceso de transición a la democracia en México.ABSTRACT: This article analyzes the local and federal state elections held in México in 1995. The author mark four principal factors in this elections. First, this elections took place in a context of economical crisis. Second, the PRI had got the worst results in his history. Third, the elections (xcepts maybe in Yucatán) were clean. At last, the election competitiveness increased because of the concentration of the opposing vote in the Partido de Acción Nacional (PAN, centre-right). The centre-leftist Partido de la Revolución Democrática (PRD) losed influence. The author conclusion is that 1995 was a year in wich the transition to democracy go faster.</p