Total hair mercury in children from a coastal population in Cananéia, São Paulo State, Brazil

Os níveis de mercúrio (Hg) total em cabelos estão diretamente relacionados à alimentação, particularmente ao consumo de peixes por populações costeiras com grande representação caiçara. No presente estudo foram avaliados os níveis de mercúrio total em cabelos de crianças com idade entre 4 e 12 anos, pertencente a três escolas públicas da cidade de Cananéia, São Paulo, Brasil. Os resultados obtidos (mediana e intervalo) para mercúrio total foram de: 0,04mg.kg-1 (0,01-0,77mg.kg-1), 0,39mg.kg-1 (< 0,01-3,33mg.kg-1) e 0,39mg.kg-1 (< 0,01-2,81mg.kg-1) considerando as escolas ES1, ES2 e ES3, respectivamente. Em geral, os valores encontrados estiveram bem abaixo do valor preconizado pela Organização Mundial da Saúde para uma população adulta não exposta ao mercúrio (2,0mg.kg-1). Os baixos valores observados e a inexistência de valores de referência para mercúrio total em cabelos de crianças brasileiras possibilitam a consideração desses valores como possível referência nacional em cabelos de populações costeiras, uma vez que foram obtidos em região de baixo impacto ambiental.Mercury (Hg) levels in hair are directly related to eating habits, especially fish consumption by coastal populations with a large contingent of traditional fishing families. This study assessed total Hg levels in children's hair. The study group was selected from three public elementary schools in Cananéia, São Paulo State, Brazil (ages 4 to 12 years). The results (median and range) for total Hg levels in children's hair were: 0.04mg.kg-1 (0.01-0.77mg.kg-1), 0.39mg.kg-1 (< 0.01-3.33mg.kg-1), and 0.39mg.kg-1 (< 0.01-2.81mg.kg-1) for schools ES1, ES2, and ES3, respectively. The values were well below the level set by World Health Organization for an adult population unexposed to Hg (2.0mg.kg-1). However, since there are no existing reference values for total Hg in children's hair, these results can be used as a contribution to establishing reference values for total hair Hg in Brazilian children living in coastal areas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Biometrics, physiology, production, and quality of Cantaloupe melons grown with saline waters under semi-arid conditions

Brazilian Northeast region emerges as both producer and exporter relevant of cantaloupe melon to several countries; however, this region is vulnerable to salinization problems due to it is inserted in a semi-arid area. This study aimed to evaluate the biometrics, physiological, production and quality of new cantaloupe melon hybrids cultivated with different saline waters under semi-arid conditions. The experiment was conducted using a drip irrigation system in a split-plot randomized design. The plot was different irrigation water electrical conductivity (ECw) levels: 0.5, 2.0, 3.5, and 5.0 dS m-1, while the subplot was two new cantaloupe melon hybrids: Zielo and SV1044. Plant biometrics (stems and leaves analysis), plant physiology (solutes accumulation and gas exchange in leaves), fruit production (number, weight, and yield), and fruit quality (physical and chemical analysis) were investigated. Stem length, leaf numbers, leaf area, and shoot dry mass decreased when ECw increased from 0.5 to 5.0 dS m-1. Also, there was an increase of Na and Cl concentration and reduction of K concentration in leaves due to ECw increase. Gas exchange reduction, losses in fruit numbers, weight, yield, and in quality were observed with the ECw increase. It is possible to conclude that the ECw increase salinizes the soil and increases Na and Cl concentration in plants causing reduction in gas exchange, in growth, and in fruit production and quality. However, the response of cultivated hybrids regarding salinity may be different, as in our study that the Zielo hybrid was less affected by the increase in ECw.Brazilian Northeast region emerges as both producer and exporter relevant of cantaloupe melon to several countries; however, this region is vulnerable to salinization problems due to it is inserted in a semi-arid area. This study aimed to evaluate the biometrics, physiological, production and quality of new cantaloupe melon hybrids cultivated with different saline waters under semi-arid conditions. The experiment was conducted using a drip irrigation system in a split-plot randomized design. The plot was different irrigation water electrical conductivity (ECw) levels: 0.5, 2.0, 3.5, and 5.0 dS m-1, while the subplot was two new cantaloupe melon hybrids: Zielo and SV1044. Plant biometrics (stems and leaves analysis), plant physiology (solutes accumulation and gas exchange in leaves), fruit production (number, weight, and yield), and fruit quality (physical and chemical analysis) were investigated. Stem length, leaf numbers, leaf area, and shoot dry mass decreased when ECw increased from 0.5 to 5.0 dS m-1. Also, there was an increase of Na and Cl concentration and reduction of K concentration in leaves due to ECw increase. Gas exchange reduction, losses in fruit numbers, weight, yield, and in quality were observed with the ECw increase. It is possible to conclude that the ECw increase salinizes the soil and increases Na and Cl concentration in plants causing reduction in gas exchange, in growth, and in fruit production and quality. However, the response of cultivated hybrids regarding salinity may be different, as in our study that the Zielo hybrid was less affected by the increase in ECw

Schistosoma mansoni venom allergen-like protein 4 (SmVAL4) is a novel lipid-binding SCP/TAPS protein that lacks the prototypical CAP motifs

Schistosomiasis is a parasitic disease that affects over 200 million people. Vaccine candidates have been identified, including Schistosoma mansoni venom allergen- like proteins (SmVALs) from the SCP/TAPS (sperm-coating protein/Tpx/antigen 5/pathogenesis related-1/Sc7) superfamily. The first SmVAL structure, SmVAL4, was refined to a resolution limit of 2.16 Å. SmVAL4 has a unique structure that could not be predicted from homologous structures, with longer loops and an unusual C-terminal extension. SmVAL4 has the characteristic ***Missing image substitution***/***Missing image substitution***-sandwich and central SCP/TAPS cavity. Furthermore, SmVAL4 has only one of the signature CAP cavity tetrad amino-acid residues and is missing the histidines that coordinate divalent cations such as Zn²⁺ in other SCP/TAPS proteins. SmVAL4 has a cavity between ***Missing image substitution***-helices 1 and 4 that was observed to bind lipids in tablysin-15, suggesting the ability to bind lipids. Subsequently, SmVAL4 was shown to bind cholesterol in vitro. Additionally, SmVAL4 was shown to complement the in vivo sterol-export phenotype of yeast mutants lacking their endogenous CAP proteins. Expression of SmVAL4 in yeast cells lacking endogenous CAP function restores the block in sterol export. These studies suggest an evolutionarily conserved lipid-binding function shared by CAP proteins such as SmVAL4 and yeast CAP proteins such as Pry1

Cluster headache and intracranial aneurysm

In the present study we describe the cases of two patients with cluster-like headache related to intracranial carotid artery aneurysm. One of these patients responded to verapamil prescription with headache resolution. In both cases the surgical clipping of the aneurysm resolved the cluster pain. These findings strongly suggest a pathophysiological link between the two conditions. The authors discuss the potential pathophysiological mechanisms underlying cluster-like headache due to intracranial carotid artery aneurysm

Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection

Schistosomiasis is a parasitic disease causing serious chronic morbidity in tropical countries. Together with the publication of the transcriptome database, a series of new vaccine candidates were proposed based on their functional classification. However, the prediction of vaccine candidates from sequence information or even by proteomics or microarrays data is somewhat speculative and there remains the considerable task of functional analysis of each new gene/protein. In this study, we present the characterization of one of these molecules, a stomatin like protein 2 (SmStoLP-2). Sequence analysis predicts signals that could contribute to protein membrane association and mitochondrial targeting, which was confirmed by differential extractions of schistosome tegument membranes and mitochondria. Additionally, confocal microscope analysis showed SmStoLP-2 present in the tegument of 7-day-old schistosomula and adult worms. Studies in patients living in endemic areas for schistosomiasis revealed high levels of IgG1, IgG2, IgG3 and IgA anti-SmStoLP-2 antibodies in individuals resistant to reinfection. Recombinant SmStoLP-2 protein, when used as vaccine, induced significant levels of protection in mice. This reduction in worm burden was associated with a typical Th1-type immune response. These results indicate that SmStoLP-2 could be useful in association with other antigens for the composition of a vaccine against schistosomiasis

A Rationale for Schistosomiasis Control in Elementary Schools of the Rainforest Zone of Pernambuco, Brazil

In 2001, a World Health Assembly resolution urged member states to ensure treatment against schistosomiasis and soil-transmitted helminthiasis in endemic areas with the goal of attaining a minimum target of at least 75% of all school-aged children by 2010. In the highly endemic Rainforest Zone of Pernambuco (ZMP), northeast Brazil, the Schistosomiasis Control Program has registered a cumulative coverage of only 20% of the population at risk, which jeopardizes the accomplishment of the minimum target for that area. Demographic and parasitological data from a representative municipality of the ZMP provide evidence that the current, community-based approach to control can be complemented with school-based actions. In the most troubled municipalities, individual diagnosis and treatment could be focused on school-aged children rather than whole populations without compromising the principles of the primary health care system. Local health and education teams should be encouraged to include school-based interventions to scale up coverage and achieve a rapid impact on infection

On the three-finger protein domain fold and CD59-like proteins in Schistosoma mansoni

Background: It is believed that schistosomes evade complement-mediated killing by expressing regulatory proteins on their surface. Recently, six homologues of human CD59, an important inhibitor of the complement system membrane attack complex, were identified in the schistosome genome. Therefore, it is important to investigate whether these molecules could act as CD59-like complement inhibitors in schistosomes as part of an immune evasion strategy. Methodology/Principal Findings: Herein, we describe the molecular characterization of seven putative SmCD59-like genes and attempt to address the putative biological function of two isoforms. Superimposition analysis of the 3D structure of hCD59 and schistosome sequences revealed that they contain the three-fingered protein domain (TFPD). However, the conserved amino acid residues involved in complement recognition in mammals could not be identified. Real-time RT-PCR and Western blot analysis determined that most of these genes are up-regulated in the transition from free-living cercaria to adult worm stage. Immunolocalization experiments and tegument preparations confirm that at least some of the SmCD59-like proteins are surface-localized; however, significant expression was also detected in internal tissues of adult worms. Finally, the involvement of two SmCD59 proteins in complement inhibition was evaluated by three different approaches: (i) a hemolytic assay using recombinant soluble forms expressed in Pichia pastoris and E. coli; (ii) complement-resistance of CHO cells expressing the respective membrane-anchored proteins; and (iii) the complement killing of schistosomula after gene suppression by RNAi. Our data indicated that these proteins are not involved in the regulation of complement activation. Conclusions: Our results suggest that this group of proteins belongs to the TFPD superfamily. Their expression is associated to intra-host stages, present in the tegument surface, and also in intra-parasite tissues. Three distinct approaches using SmCD59 proteins to inhibit complement strongly suggested that these proteins are not complement inhibitors and their function in schistosomes remains to be determined.Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP, Grant Number:04/12872-3)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Institute of Health, National Institute of Allergy and Infectious Diseases (NIH-NIAID), Grant AI-095893NIH-NIAID Grant AI-056273FAPESP 00/11624-