A Negative Feedback Loop Regulates Integrin Inactivation and Promotes Neutrophil Recruitment to Inflammatory Sites

International audienc

INFLUÊNCIA DA OBESIDADE SARCOPÊNICA NO RISCO DE QUEDAS EM IDOSOS: UMA REVISÃO SISTEMÁTICA

Aim. To assess the influence of Sarcopenic Obesity (SO) on the risk of falls among the elderly, showing the impact of this association on the quality of life, health and autonomy of this population. Methods. This is a systematic review in which ​​studies indexed in the Pubmed, Scopus and BVS databases were searched using the descriptors (Sarcopenia AND Obesity OR Sarcopenic Obesity) AND (Elderly OR Elderly OR Elderly) AND (Accidental falls).Were read from the articles: title and abstract. Duplicates, articles that do not fit the proposed objective, literary reviews, books, conference abstracts and editorials, as well as publications prior to 2014 were eliminated. Two independent reviewers assessed the studies eligibility. The full text version was read if at least one of the reviewers considered the study eligible. Data was independently extracted from each study by the authors. Results. A total of ten studies met the criteria to be included in this review, comprehending a total of 22,418 participants and their respective data. The prevalence of falls in patients with SO varied between 29.47% and 60.53%, the Odds Rattio between the risk of falls and the defining components of SO varied between 1.05 and 2.64. Conclusions. A relationship between SO cases and the increase in the number of falls was noted from the selected studies, showing a change in the quality of life of the elderly and a possible increase in morbidity and mortality.Objetivo: Avaliar a influência da obesidade sarcopênica (OS) no risco de quedas em pessoas idosas, evidenciando o impacto dessa associação na qualidade de vida, saúde e autonomia dessa população. Métodos: Trata-se de uma revisão sistemática, na qual foram pesquisados estudos indexados nas bases PubMed, Scopus e BVS a partir dos descritores (Sarcopenia AND Obesity OR Sarcopenic obesity) AND (Aged OR Elderly OR Older people) AND (Accidental falls). Foram lidos o título e o resumo dos artigos. Duplicidades, artigos que não se adequaram ao objetivo proposto, revisões literárias, livros, resumos de conferências e editoriais, bem como publicações anteriores a 2014, foram eliminados. Dois revisores avaliaram independentemente a elegibilidade dos estudos. A versão completa do texto foi lida se pelo menos um dos revisores considerasse o estudo elegível. Os dados foram extraídos de cada estudo de forma independente pelos autores. Resultados: Dez estudos contemplaram os critérios para serem incluídos nesta revisão, compreendendo um total de 22.418 participantes e seus respectivos dados. A prevalência de quedas em pacientes com OS variou entre 29,47% e 60,53%, e o Odds Ratio entre o risco de quedas e os componentes definidores de OS variou entre 1,05 e 2,64. Conclusão: Notou-se uma relação significativa entre os casos de OS e o aumento do número de quedas a partir dos estudos selecionados, evidenciando uma alteração na qualidade de vida dos idosos e possível aumento de morbimortalidade

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker initiation on organ support-free days in patients hospitalized with COVID-19

IMPORTANCE Overactivation of the renin-angiotensin system (RAS) may contribute to poor clinical outcomes in patients with COVID-19. Objective To determine whether angiotensin-converting enzyme (ACE) inhibitor or angiotensin receptor blocker (ARB) initiation improves outcomes in patients hospitalized for COVID-19. DESIGN, SETTING, AND PARTICIPANTS In an ongoing, adaptive platform randomized clinical trial, 721 critically ill and 58 non–critically ill hospitalized adults were randomized to receive an RAS inhibitor or control between March 16, 2021, and February 25, 2022, at 69 sites in 7 countries (final follow-up on June 1, 2022). INTERVENTIONS Patients were randomized to receive open-label initiation of an ACE inhibitor (n = 257), ARB (n = 248), ARB in combination with DMX-200 (a chemokine receptor-2 inhibitor; n = 10), or no RAS inhibitor (control; n = 264) for up to 10 days. MAIN OUTCOMES AND MEASURES The primary outcome was organ support–free days, a composite of hospital survival and days alive without cardiovascular or respiratory organ support through 21 days. The primary analysis was a bayesian cumulative logistic model. Odds ratios (ORs) greater than 1 represent improved outcomes. RESULTS On February 25, 2022, enrollment was discontinued due to safety concerns. Among 679 critically ill patients with available primary outcome data, the median age was 56 years and 239 participants (35.2%) were women. Median (IQR) organ support–free days among critically ill patients was 10 (–1 to 16) in the ACE inhibitor group (n = 231), 8 (–1 to 17) in the ARB group (n = 217), and 12 (0 to 17) in the control group (n = 231) (median adjusted odds ratios of 0.77 [95% bayesian credible interval, 0.58-1.06] for improvement for ACE inhibitor and 0.76 [95% credible interval, 0.56-1.05] for ARB compared with control). The posterior probabilities that ACE inhibitors and ARBs worsened organ support–free days compared with control were 94.9% and 95.4%, respectively. Hospital survival occurred in 166 of 231 critically ill participants (71.9%) in the ACE inhibitor group, 152 of 217 (70.0%) in the ARB group, and 182 of 231 (78.8%) in the control group (posterior probabilities that ACE inhibitor and ARB worsened hospital survival compared with control were 95.3% and 98.1%, respectively). CONCLUSIONS AND RELEVANCE In this trial, among critically ill adults with COVID-19, initiation of an ACE inhibitor or ARB did not improve, and likely worsened, clinical outcomes. TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT0273570

Baa! A procedural game based on real-time flocking behaviour

Simulating the natural movement of large groups of flocking animals has been a growing field of research within computer science since the 1980’s, largely because of its potential in performance optimisation. The purpose of this bachelor’s thesis is to combine this existing research in flocking behaviour with gameplay design and optimisation to create a procedural game with flocking as its main feature. To accomplish this, Unreal Engine 4 and C++ has been used as a working environment. The result is an optimised game where roughly 700 sheep in a flock can be present without a significant performance loss. The actual number of sheep used in the game, however, is significantly lower to make the game more playable

Elektrisk effektanalys av vårdcentraler

Med rådande kapacitetsbrist i Uppsala finns det incitament att se över det elektriska effektuttaget. Region Uppsala äger fastigheter såsom vårdlokaler med varierande elektriskt effektbehov. Utöver variationen mellan fastigheter så varierar det elektriska effektuttaget för en och samma byggnad kraftigt under dygnet. Där finns det ett behov för större förståelse för förbrukningen. Detta projektarbete går ut på att göra en elektrisk effektanalys på vårdcentralerna i Heby och Årsta för att utreda tillgängliga alternativ för att minska det elektriska effektuttaget av vårdcentraler i Region Uppsala. Projektarbetet utförs endast utifrån ett tekniskt perspektiv. Resultaten visar att de största lasterna på vardera vårdcentral är undercentral, belysning och ventilation. Det är möjligt att sänka den elektriska effektkonsumtionen med ungefär 30% i både Heby och Årsta. Det finns många laster som går att ändra drifttider till lägre belastningstid på dygnet.With the current power transmission shortage in Uppsala, there are incentives to look over the electrical power usage. Region Uppsala owns properties such as health care centers with varying electrical power demand. In addition to variations in demand between different properties, one single building can have widely varying electrical power demands over 24 hours. There is a need to better understand the electrical power usage. The purpose of this project is to conduct an electrical power analysis for the health care centers in Heby and Årsta, and to investigate available alternatives to reduce the electrical power usage in health care centers in Region Uppsala. The project is solely conducted from a technical perspective. The results show that the largest loads at each health center are the mechanical room, lighting and ventilation. It is possible to reduce the electrical power consumption by about 30 % in both Heby and Årsta. There are several loads that can be moved to lower load times during the day

INFLUÊNCIA DA OBESIDADE SARCOPÊNICA NO RISCO DE QUEDAS EM IDOSOS: UMA REVISÃO SISTEMÁTICA

Aim. To assess the influence of Sarcopenic Obesity (SO) on the risk of falls among the elderly, showing the impact of this association on the quality of life, health and autonomy of this population. Methods. This is a systematic review in which ​​studies indexed in the Pubmed, Scopus and BVS databases were searched using the descriptors (Sarcopenia AND Obesity OR Sarcopenic Obesity) AND (Elderly OR Elderly OR Elderly) AND (Accidental falls).Were read from the articles: title and abstract. Duplicates, articles that do not fit the proposed objective, literary reviews, books, conference abstracts and editorials, as well as publications prior to 2014 were eliminated. Two independent reviewers assessed the studies eligibility. The full text version was read if at least one of the reviewers considered the study eligible. Data was independently extracted from each study by the authors. Results. A total of ten studies met the criteria to be included in this review, comprehending a total of 22,418 participants and their respective data. The prevalence of falls in patients with SO varied between 29.47% and 60.53%, the Odds Rattio between the risk of falls and the defining components of SO varied between 1.05 and 2.64. Conclusions. A relationship between SO cases and the increase in the number of falls was noted from the selected studies, showing a change in the quality of life of the elderly and a possible increase in morbidity and mortality.Objetivo: Avaliar a influência da obesidade sarcopênica (OS) no risco de quedas em pessoas idosas, evidenciando o impacto dessa associação na qualidade de vida, saúde e autonomia dessa população. Métodos: Trata-se de uma revisão sistemática, na qual foram pesquisados estudos indexados nas bases PubMed, Scopus e BVS a partir dos descritores (Sarcopenia AND Obesity OR Sarcopenic obesity) AND (Aged OR Elderly OR Older people) AND (Accidental falls). Foram lidos o título e o resumo dos artigos. Duplicidades, artigos que não se adequaram ao objetivo proposto, revisões literárias, livros, resumos de conferências e editoriais, bem como publicações anteriores a 2014, foram eliminados. Dois revisores avaliaram independentemente a elegibilidade dos estudos. A versão completa do texto foi lida se pelo menos um dos revisores considerasse o estudo elegível. Os dados foram extraídos de cada estudo de forma independente pelos autores. Resultados: Dez estudos contemplaram os critérios para serem incluídos nesta revisão, compreendendo um total de 22.418 participantes e seus respectivos dados. A prevalência de quedas em pacientes com OS variou entre 29,47% e 60,53%, e o Odds Ratio entre o risco de quedas e os componentes definidores de OS variou entre 1,05 e 2,64. Conclusão: Notou-se uma relação significativa entre os casos de OS e o aumento do número de quedas a partir dos estudos selecionados, evidenciando uma alteração na qualidade de vida dos idosos e possível aumento de morbimortalidade