Groundwater animals

Groundwater animals are adapted to live in environments with no light and limited nutrients, They can provide insights into fundamental questions of evolution, ecology and biodiversity. They also have an important role to play in informing the reconstruction of past changes in geomorphology and climate, and can be used for characterising aquifers. The BGS is undertaking a systematic survey of selected areas and lithologies in the UK where groundwater animals have not been investigated. This is important because little is known about groundwater ecosystems in the UK despite the unique contribution to biodiversity made by these animals. Groundwater organisms are also thought to provide ecosystem services by means of their role in nutrient cycling and natural remediation of pollutants in the subsurface. They may also be useful indicators of human impacts on groundwaters

Silicate determination in sea water: toward a reagentless electrochemical method

ilicate has been determined in sea water by four different electrochemical methods based on the detection of the silicomolybdic complex formed in acidic media by the reaction between silicate and molybdenum salts. The first two methods are based on the addition of molybdate and protons in a seawater sample in an electrochemical cell. Cyclic voltammetry presents two reduction and two oxidation peaks giving four values of the concentration and therefore increasing the precision. Then chronoamperometry is performed on an electrode held at a constant potential. A semi-autonomous method has been developed based on the electrochemical anodic oxidation of molybdenum, the complexation of the oxidation product with silicate and the detection of the complex by cyclic voltammetry. This method is tested and compared with the classical colorimetric one during ANT XXIII/3 cruise across Drake Passage (January–February 2006). The detection limit is 1 μM and the deviation between both methods is less than 3% for concentrations higher than 10 μM. Finally a complete reagentless method with a precision of 2.6% is described based on the simultaneous formation of the molybdenum salt and protons in a divided electrochemical cell. This latter method should be very useful for developing a reagentless sensor suitable for long term in situ deployments on oceanic biogeochemical observatories

Obligate groundwater crustaceans mediate biofilm interactions in a subsurface food web

Food webs in groundwater ecosystems are dominated by only a few top-level consumers, mainly crustaceans. These obligate groundwater dwellers—or stygobites—clearly interact with groundwater biofilm, but it is uncertain whether they affect the abundance and structure of biofilm assemblages. We hypothesized that crustacean stygobites would reduce bacteria and protozoan abundance and alter biofilm assemblage structure. We also hypothesized that high densities of stygobites would remove more bacteria and protozoa than would low densities, and that this difference would become more pronounced over time. First, we established that the amphipods Niphargus fontanus and Niphargus kochianus both ingest biofilm by examining their gut contents. We then conducted 2 microcosm experiments. The 1st experiment showed that both N. fontanus and the isopod Proasellus cavaticus increased protozoan abundance but that bacterial abundance was only slightly reduced in the presence of P. cavaticus. In the 2nd experiment, we determined how zero, low, and high densities of N. kochianus affected the biofilm. The high-density treatment of N. kochianus had significantly higher protozoan abundance than the control and the low-density treatment, and high densities of N. kochianus significantly increased the relative proportions of small- and medium-sized bacteria over time compared with controls. Our controlled microcosm experiments demonstrate that macroinvertebrate stygobites can influence groundwater biofilm assemblages, although the exact mechanisms are not clear. These results support the hypothesis that stygobites influence essential ecosystem services supplied by groundwater ecosystems

Karst hydrogeology of the Chalk and implications for groundwater protection

The Chalk is an unusual karst aquifer with limited cave development, but extensive networks of smaller solutional conduits and fissures enabling rapid groundwater flow. Small-scale karst features (stream sinks, dolines, dissolution pipes, and springs) are common, with hundreds of stream sinks recorded. Tracer velocities from 27 connections between stream sinks and springs have median and mean velocities of 4700 and 4600 m d−1. Tests to abstraction boreholes also demonstrate very rapid velocities of thousands of metres per day. Natural gradient tests from observation boreholes have rapid velocities of hundreds of metres per day. There is strong geological control on karst with dissolution focused on stratigraphical inception horizons. Surface karst features are concentrated near the Paleogene boundary, or where thin superficial cover occurs, but rapid groundwater flow is also common in other areas. The Chalk has higher storage and contaminant attenuation than classical karst, but recharge, storage and flow are influenced by karst. Point recharge through stream sinks, dolines, losing rivers, vertical solutional fissures, and soakaways enables rapid unsaturated zone flow. Saturated zone networks of solutional fissures and conduits create vulnerability to subsurface activities, and enable long distance transport of point source and diffuse pollutants, which may be derived from outside modelled catchment areas and source protection zones

Meltwater flow through a rapidly deglaciating glacier and foreland catchment system: Virkisjökull, SE Iceland

Virkisjökull is a rapidly retreating glacier in south-east Iceland. A proglacial lake has formed in the last ten years underlain by buried ice. In this study we estimate water velocities through the glacier, proglacial foreland and proglacial river using tracer tests and continuous meltwater flow measurements. Tracer testing from a glacial moulin to the glacier outlet in September 2013 demonstrated a rapid velocity of 0.58 m s�1. This was comparable to the velocity within the proglacial river, also estimated from tracer testing. A subsequent tracer test from the same glacial moulin under low flow conditions in May 2014 demonstrated a slower velocity of 0.07 m s�1. The glacier outlet river sinks back into the buried ice, and a tracer test from this sink point through the proglacial foreland to the meltwater river beyond the lake indicated a velocity of 0.03 m s�1,suggesting that an ice conduit system within the buried ice is transferring water rapidly beneath the lake. Ground penetrating radar profiles confirm the presence of this buried conduit system. This study provides an example of rapid deglaciation being associated with extensive conduit systems that enable rapid meltwater transfer from glaciers through the proglacial area to meltwater rivers

Comparing the fate and transport of MS2 bacteriophage and sodium fluorescein in a karstic chalk aquifer

Groundwater flow and contaminant migration tracing is a vital method of identifying and characterising pollutant source-pathway-receptor linkages in karst aquifers. Bacteriophages are an attractive alternative tracer to non-reactive fluorescent dye tracers, as high titres (>10¹² pfu mL−¹) can be safely released into the aquifer, offering improved tracer detectability. However, the interpretation of bacteriophage tracer breakthrough curves is complicated as their fate and transport are impacted by aquifer physicochemical conditions. A comparative tracer migration experiment was conducted in a peri-urban catchment in southeast England to characterise the behaviour of MS2 bacteriophage relative to sodium fluorescein dye in a karstic chalk aquifer. Tracers were released into a stream sink and detected at two abstraction boreholes located 3 km and 10 km away. At both sites, the loss of MS2 phage greatly exceeded that of the solute tracer. In contrast, the qualitative shape of the dye and phage breakthrough curves were visually very similar, suggesting that the bacteriophage arriving at each site was governed by comparable transport parameters to the non-reactive dye tracer. The colloid filtration theory was applied to explain the apparent contradiction of comparable tracer breakthrough patterns despite massive phage losses in the subsurface. One-dimensional transport models were also fitted to each breakthrough curve to facilitate a quantitative comparison of the transport parameter values. The model results suggest that the bacteriophage migrates through the conduit system slightly faster than the fluorescent dye, but that the former is significantly less dispersed. These results suggest that whilst the bacteriophage tracer cannot be used to predict receptor concentrations from transport via karstic flow paths, it can provide estimates for groundwater flow and solute contaminant transit times. This study also provides insight into the attenuation and transport of pathogenic viruses in karstic chalk aquifers

Defining geo-habitats for groundwater ecosystem assessments: an example from England and Wales (UK)

Groundwater ecosystems comprising micro-organisms and metazoans provide an important contribution to global biodiversity. Their complexity depends on geology, which determines the physical habitat available, and the chemical conditions within it. Despite this, methods of classifying groundwater habitats using geological data are not well established, and researchers have called for higher resolution habitat frameworks. A novel habitat typology for England and Wales (UK) is proposed, which distinguishes 11 geological habitats (geo-habitats) on hydrogeological principles and maps their distribution. Hydrogeological and hydrochemical data are used to determine the characteristics of each geo-habitat, and demonstrate their differences. Using these abiotic parameters, a new method to determine abiotic habitat quality is then developed. The geo-habitats had significantly different characteristics validating the classification system. Karstic and porous habitats were generally of higher quality than fractured habitats. All geo-habitats were highly heterogeneous, containing both high quality habitat patches that are likely to be suitable for fauna, and areas of low quality that may limit faunal distributions. Overall, 70 % of England and Wales are covered by lower quality fractured habitats, with only 13 % covered by higher quality habitats. The main areas of high quality habitats occur in central England as north-south trending belts, possibly facilitating dispersal along this axis. They are separated by low quality geo-habitats that may prevent east-west dispersal of fauna. In south-west England and in Wales suitable geo-habitats occur as small isolated patches. Overall, this paper provides a new national-scale typology that is adaptable for studies in other geographic areas

Contextualizing Violence Prevention – How Contextual Aspects Influence the Implementation of a Violence Prevention Initiative in Prisons and Psychiatry

The aim of this study was to investigate which contextual aspects and mechanisms are decisive in the implementation of an integrated, tailored intervention to prevent violence and threats of violence from patients or inmates towards employees in psychiatric units and in prisons and detention centers. Based on a standardized implementation degree assessment of fidelity, reach and dose delivered, we selected two workplace cases from each sector, one with a high and one with a low implementation degree. Using a realist evaluation framework, we conducted a thematic analysis of the four selected workplace cases to identify prevalent contextual aspects and mechanisms underlying the implementation degree. We found that prioritization, synergy with parallel change processes, and intervention fit, were decisive mechanisms for the successful implementation of the intervention. We also found that lack of resources (staff instability, insufficient time, poor mental resources) and resource-demanding parallel change processes, were contextual aspects that “blocked” all mechanisms. That is, when resources were not available, none of the mechanisms for implementing the intervention was activated. Our findings point to the importance of investigating the role of contextual aspects when assessing the effectiveness of organizational interventions

Determinants of participation in a web-based health risk assessment and consequences for health promotion programs

Background: The health risk assessment (HRA) is a type of health promotion program frequently offered at the workplace. Insight into the underlying determinants of participation is needed to evaluate and implement these interventions. Objective: To analyze whether individual characteristics including demographics, health behavior, self-rated health, and work-related factors are associated with participation and nonparticipation in a Web-based HRA. Methods: Determinants of participation and nonparticipation were investigated in a cross-sectional study among individuals employed at five Dutch organizations. Multivariate logistic regression was performed to identify determinants of participation and nonparticipation in the HRA after controlling for organization and all other variables. Results: Of the 8431 employees who were invited, 31.9% (2686/8431) enrolled in the HRA. The online questionnaire was completed by 27.2% (1564/5745) of the nonparticipants. Determinants of participation were some periods of stress at home or work in the preceding year (OR 1.62, 95% CI 1.08-2.42), a decreasing number of weekdays on which at least 30 minutes were spent on moderate to vigorous physical activity (ORdayPA0.84, 95% CI 0.79-0.90), and increasing alcohol consumption. Determinants of nonparticipation were less-than-positive self-rated health (poor/very poor vs very good, OR 0.25, 95% CI 0.08-0.81) and tobacco use (at least weekly vs none, OR 0.65, 95% CI 0.46-0.90). Conclusions: This study showed that with regard to isolated health behaviors (insufficient physical activity, excess alcohol consumption, and stress), those who could benefit most from the HRA were more likely to participate. However, tobacco users and those who rate

Patterns of at-sea behaviour at a hybrid zone between two threatened seabirds

Patterns of behavioural variation and migratory connectivity are important characteristics of populations, particularly at the edges of species distributions, where processes involved in influencing evolutionary trajectories, such as divergence, mutual persistence, and natural hybridization, can occur. Here, we focused on two closely related seabird species that breed in the Mediterranean: Balearic shearwaters (Puffinus mauretanicus) and Yelkouan shearwaters (Puffinus yelkouan). Genetic and phenotypic evidence of hybridization between the two species on Menorca (the eastern and westernmost island in the breeding ranges of the two shearwaters, respectively) has provided important insights into relationships between these recently diverged species. Nevertheless, levels of behavioural and ecological differentiation amongst these populations remain largely unknown. Using geolocation and stable isotopes, we compared the at-sea movement behaviour of birds from the Menorcan ‘hybrid’ population with the nearest neighbouring populations of Balearic and Yelkouan shearwaters. The Menorcan population displayed a suite of behavioural features intermediate to those seen in the two species (including migration strategies, breeding season movements and limited data on phenology). Our findings provide new evidence to support suggestions that the Menorcan population is admixed, and indicate a role of non-breeding behaviours in the evolutionary trajectories of Puffinus shearwaters in the Mediterranean