Semences et séquences génétiques en open source ? Expériences récentes et stratégies futures

Le concept d'open source appliqué aux semences végétales a un avenir prometteur. Ce concept inverse la logique du système de propriété intellectuelle pour une ressource renouvelable que l'on rend disponible en la sortant du domaine exclusif de la propriété intellectuelle. Aujourd'hui, les instruments juridiques font encore défaut pour établir un cadre légal complet. Toutefois, le concept d'open source, que l'on pourrait traduire ici par licence libre et ouverte, engendre au fil du temps une masse critique d'utilisateurs et de soutiens qui entraîne une légitimité sociale grandissante. À terme, cette légitimité pourrait renforcer le pouvoir juridique. L'extension future à d'autres matériels dans différents contextes doit être réfléchie en s'appuyant sur les expériences actuelles de semences sous licences libres et ouvertes conduites aux États-Unis, en Europe et en Afrique

The 2019 review of IPBES and future priorities: Reaching beyond assessment to enhance policy impact

The Intergovernmental Science–Policy Platform on Biodiversity and Ecosystem Services (IPBES) is an independent scientific body focused on assessing the state of the world's ecosystem services and biodiversity. IPBES members agreed in 2017 that a review of the Platform's first work programme should be undertaken by an independent panel examining all aspects of IPBES' work – including implementation of the four functions of IPBES; policies, operating principles and procedures; governance structure and arrangements; communication, stakeholder engagement and partnerships; and funding mechanisms. The review found that for IPBES to have its anticipated transformative impact: All four functions of IPBES (i.e. assessment, knowledge generation, policy support, capacity building), with better communication, must be significantly strengthened, integrated and delivered together; The policy aspects of IPBES work need to be strengthened and greater emphasis needs to be placed on the co-design and co-production of assessments; A more strategic and collaborative approach to stakeholders is needed; and IPBES must develop a more sustainable financial base. Given those changes, IPBES, as an embryonic boundary organization, can become the key influencing organization in the global landscape of biodiversity and ecosystem services organizations, helping thus to catalyze transformative change in the relationship between people and the rest of nature

Post COVID-19 implications on genetic diversity and genomics research & innovation: A call for governance and research capacity. White paper.

This is the final version. Available from the Food and Agriculture Organisation via the DOI in this record. At a time of significant technological change and digitization in the biological sciences, the COVID19 pandemic has highlighted again the inequities in the research and innovation ecosystem. Based on a consultation with an internationally diverse group of stakeholders from multiple fields and professions, and on a broadly representative set of case studies, this report offers a new approach to the global governance of genetic diversity and genomic research and innovation. We recommend that in addition to the many valuable efforts at the macro-policy level and at the micro-level of projects, teams and organizations, the global community concerned with genetic diversity and genomic research and innovation should devise and implement a meso-level initiative that includes three main components: 1. First, it should establish a new professional capacity to govern research and innovation at the meso-level. Governance capacity, built through a networked community of practice, has the benefit of connecting and integrating macrolevel policy intentions with micro-level actions. It facilitates a consistent professional basis from which local and regional level flexibilities can generate new norms of reflection that better integrate multiple synergies, reconcile tensions, recognize inequities, and redress persistent inequalities. 2. Second, the global community should redouble efforts to build research capacity in genomic research and innovation in the Global South and for Indigenous Peoples. Such an effort should be focused on broader programmatic objectives that facilitate cross-national and cross-regional collaboration, as well as enhancing research communities in the Global South and in Indigenous communities. Together, the twin capacities of governance and research can reduce power differentials among diverse actors and support crisisbased imperatives for data openness. 3. Third, we recommend that existing global policy frameworks interface with research governance and capacity investment. This meso-level approach should gain the commitment and support from national and international policy bodies, embedded within existing specific issue-areas (health, agriculture, environment). A new approach, one that can better respond to global crises though more open, inclusive and equitable participation in research and innovation, is necessary to resolve the tensions among openness, innovation and equity that the current discourse on genetic diversity reiterates. Failure to systematically address the social and technical governance challenges will result in further fragmentation, inequity and vulnerability for decades to come. Conversely, investing in the current historical moment of the pandemic to build twin capacities for meso-level governance and research is poised to prevent and/or reduce the impact of future ecological crises, while contributing to planetary sustainability and prosperity in the 21st century for current and future generations.European CommissionAlan Turing Institut

A systematic quantitative literature review of aquaculture genetic resource access and benefit sharing

The Convention on Biological Diversity provides a framework for countries to implement laws regulating the access, use and exchange of genetic resources, including how users and providers share the benefits from their use. While the international community has been preoccupied with resolving the unintended effects of access and benefit sharing (ABS) on domestication in agriculture for the past 25 years, its far-reaching consequences for global aquaculture has only recently dawned on policymakers, aquaculture producers and researchers. Using a systematic quantitative literature review methodology, we analysed the trends, biases and gaps in the ABS literature. Only 5% of the ABS literature related to the use and exchange of aquaculture genetic resources. Most of this literature related to use in developing countries or global use, but its authors were predominantly from developed countries. The literature covered a narrow range of countries (7) and regions (3), a narrow range of taxonomic groups (9) and a narrow range of uses. Given that aquaculture is the fastest growing global food production sector with products primarily from developing countries using over 580 species, there are significant gaps in aquaculture-related ABS literature. We conclude that the sector needs urgent analyses on the consequences of ABS restrictions, obligations and opportunities for its early stages of domestication and product development. We recommend priority areas for attention to ensure that rapidly evolving national ABS laws take into account the special characteristics and needs of the aquaculture sector

MIR-99a and MIR-99b Modulate TGF-β Induced Epithelial to Mesenchymal Plasticity in Normal Murine Mammary Gland Cells

Epithelial to mesenchymal transition (EMT) is a key process during embryonic development and disease development and progression. During EMT, epithelial cells lose epithelial features and express mesenchymal cell markers, which correlate with increased cell migration and invasion. Transforming growth factor-β (TGF-β) is a multifunctional cytokine that induces EMT in multiple cell types. The TGF-β pathway is regulated by microRNAs (miRNAs), which are small non-coding RNAs regulating the translation of specific messenger RNAs

Residual hepatocellular carcinoma after oxaliplatin treatment has increased metastatic potential in a nude mouse model and is attenuated by Songyou Yin

<p>Abstract</p> <p>Background</p> <p>The opposite effects of chemotherapy, which enhance the malignancy of treated cancers such as hepatocellular carcinoma (HCC), are not well understood. We investigated this phenomenon and corresponding mechanisms to develop a novel approach for improving chemotherapy efficacy in HCC.</p> <p>Methods</p> <p>Human hepatocellular carcinoma cell lines HepG2 (with low metastatic potential) and MHCC97L (with moderate metastatic potential) were used for the in vitro study. An orthotopic nude mouse model of human HCC was developed using MHCC97L cells. We then assessed the metastatic potential of surviving tumor cells after in vitro and in vivo oxaliplatin treatment. The molecular changes in surviving tumor cells were evaluated by western blot, immunofluorescence, and immunohistochemistry. The Chinese herbal extract Songyou Yin (composed of five herbs) was investigated in vivo to explore its effect on the metastatic potential of oxaliplatin-treated cancer cells.</p> <p>Results</p> <p>MHCC97L and HepG2 cells surviving oxaliplatin treatment showed enhanced migration and invasion in vitro. Residual HCC after in vivo oxaliplatin treatment demonstrated significantly increased metastasis to the lung (10/12 vs. 3/12) when re-inoculated into the livers of new recipient nude mice. Molecular changes consistent with epithelial-mesenchymal transition (EMT) were observed in oxaliplatin-treated tumor tissues and verified by in vitro experiments. The Chinese herbal extract Songyou Yin (4.2 and 8.4 g/kg) attenuated EMT and inhibited the enhanced metastatic potential of residual HCC in nude mice (6/15 vs. 13/15 and 3/15 vs. 13/15, respectively).</p> <p>Conclusions</p> <p>The surviving HCC after oxaliplatin treatment underwent EMT and demonstrated increased metastatic potential. Attenuation of EMT by Songyou Yin may improve the efficacy of chemotherapy in HCC.</p

MicroRNA-155 Deficiency Attenuates Liver Steatosis and Fibrosis without Reducing Inflammation in a Mouse Model of Steatohepatitis

BACKGROUND & AIM: MicroRNAs (miRs) regulate hepatic steatosis, inflammation and fibrosis. Fibrosis is the consequence of chronic tissue damage and inflammation. We hypothesized that deficiency of miR-155, a master regulator of inflammation, attenuates steatohepatitis and fibrosis. METHODS: Wild type (WT) and miR-155-deficient (KO) mice were fed methionine-choline-deficient (MCD) or -supplemented (MCS) control diet for 5 weeks. Liver injury, inflammation, steatosis and fibrosis were assessed. RESULTS: MCD diet resulted in steatohepatitis and increased miR-155 expression in total liver, hepatocytes and Kupffer cells. Steatosis and expression of genes involved in fatty acid metabolism were attenuated in miR-155 KO mice after MCD feeding. In contrast, miR-155 deficiency failed to attenuate inflammatory cell infiltration, nuclear factor kappa beta (NF-kappaB) activation and enhanced the expression of the pro-inflammatory cytokines tumor necrosis factor alpha (TNFalpha) and monocyte chemoattractant protein-1 (MCP1) in MCD diet-fed mice. We found a significant attenuation of apoptosis (cleaved caspase-3) and reduction in collagen and alpha smooth muscle actin (alphaSMA) levels in miR-155 KO mice compared to WTs on MCD diet. In addition, we found attenuation of platelet derived growth factor (PDGF), a pro-fibrotic cytokine; SMAD family member 3 (Smad3), a protein involved in transforming growth factor-beta (TGFbeta) signal transduction and vimentin, a mesenchymal marker and indirect indicator of epithelial-to-mesenchymal transition (EMT) in miR-155 KO mice. Nuclear binding of CCAAT enhancer binding protein beta (C/EBPbeta) a miR-155 target involved in EMT was significantly increased in miR-155 KO compared to WT mice. CONCLUSIONS: Our novel data demonstrate that miR-155 deficiency can reduce steatosis and fibrosis without decreasing inflammation in steatohepatitis

Evidence for Positive Selection on a Number of MicroRNA Regulatory Interactions during Recent Human Evolution

MicroRNA (miRNA)–mediated gene regulation is of critical functional importance in animals and is thought to be largely constrained during evolution. However, little is known regarding evolutionary changes of the miRNA network and their role in human evolution. Here we show that a number of miRNA binding sites display high levels of population differentiation in humans and thus are likely targets of local adaptation. In a subset we demonstrate that allelic differences modulate miRNA regulation in mammalian cells, including an interaction between miR-155 and TYRP1, an important melanosomal enzyme associated with human pigmentary differences. We identify alternate alleles of TYRP1 that induce or disrupt miR-155 regulation and demonstrate that these alleles are selected with different modes among human populations, causing a strong negative correlation between the frequency of miR-155 regulation of TYRP1 in human populations and their latitude of residence. We propose that local adaptation of microRNA regulation acts as a rheostat to optimize TYRP1 expression in response to differential UV radiation. Our findings illustrate the evolutionary plasticity of the microRNA regulatory network in recent human evolution