Item-by-item sampling for promotional purposes

This is an accepted manuscript of an article accepted for publication by Taylor & Francis in Quality Technology & Quantitative Management on 7 June 2017, available online at doi: http://www.tandfonline.com/doi/abs/10.1080/16843703.2017.1335494In this paper we present a method for sampling items that are checked on a pass/fail basis, with a view to a statement being made about the success/failure rate for the purposes of promoting an organisation’s product/service to potential clients/customers. Attention is paid to the appropriate use of statistical phrases for the statements and this leads to the use of Bayesian credible intervals, thus it exceeds what can achieved with standard acceptance sampling techniques. The hypergeometric distribution is used to calculate successive stopping rules so that the resources used for sampling can be minimised. Extensions to the sampling procedure are considered to allow the potential for stronger and weaker statements to be made as sampling progresses. The relationship between the true error rate and the probabilities of making correct statements is discussed.Peer reviewedFinal Accepted Versio

Normal Enough? Tools to Aid Decision Making

This document is the Accepted Manuscript version of the following article: Neil H. Spencer, Margaret Lay and Lindsey Kevan de Lopez, ‘Normal enough? Tools to aid decision making’, International Journal of Social Research Methodology, Vol. 20(2): 167-179, 2017, DOI: https://doi.org/10.1080/13645579.2016.1155379. Published by Taylor & Francis.When undertaking quantitative hypothesis testing, social researchers need to decide whether the data with which they are working is suitable for parametric analyses to be used. When considering the relevant assumptions they can examine graphs and summary statistics but the decision making process is subjective and must also take into account the robustness of the proposed tests to deviations from the assumptions. We review the contemporary advice on this issue available to researchers and look back to the roots of hypothesis testing and associated work undertaken by eminent statisticians since the 1930s. From this we create a set of flow charts to give researchers tools they can use to make decisions in a more objective manner.Peer reviewedFinal Accepted Versio

High-Energy Astrophysics in the 2020s and Beyond

With each passing decade, we gain new appreciation for the dynamic, connected, and often violent nature of the Universe. This reality necessarily places the study of high-energy processes at the very heart of modern astrophysics. This White Paper illustrates the central role of high-energy astrophysics to some of the most pressing astrophysical problems of our time, the formation/evolution of galaxies, the origin of the heavy elements, star and planet formation, the emergence of life on exoplanets, and the search for new physics. We also highlight the new connections that are growing between astrophysicists and plasma physicists. We end with a discussion of the challenges that must be addressed to realize the potential of these connections, including the need for integrated planning across physics and astronomy programs in multiple agencies, and the need to foster the creativity and career aspirations of individual scientists in this era of large projects.Comment: Astro2020 White Paper submissio

Is it possible to store spotted wolffish (Anarhichas minor) sperm by refrigeration?

Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish sperm has the particularity that is already motile on stripping, and currently it is not possible to immobilize and reactivate. Thus, sperm refrigeration protocols should focus in extending this motility period that usually lasts up to 2 days. In a first experiment, we evaluated the possibility that the motility period of the sperm was limited by contamination with urine. The urea concentration in the sperm obtained both by stripping (17.10 ± 1.98 mg/dL) and directly from the testis (12.59 ± 2.37 mg/dL) was similar (p > 0.05), which indicate that the sperm collection method used avoid contamination with urine. Afterwards, we tested the possibility that the sperm motility period was limited by energy stores. The ATP concentration (initial value 5.65 ± 0.86 nmol/109 cells) remained stable (p = 0.099) during 30 h after sperm collection, and similar values (p = 0.329) were recorded at end of sperm storage in both diluted (3.88 ± 1.35 nmol/109 cells) and undiluted samples (4.76 ± 1.08 nmol/109). This indicates that the low intracellular ATP consumption, derived from the slow sperm motility, can probably be compensated rapidly enough by mitochondrial synthesis of ATP in the spotted wolffish sperm. In both experiments, diluted sperm kept higher percentage of motile cells during the storage time.publishedVersionUnit License Agreemen

A transgenic Camelina sativa seed oil effectively replaces fish oil as a dietary source of eicosapentaenoic acid in mice

Background: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed. Objective: The main objective was to investigate the ability of genetically engineered Camelina sativa (20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals. Methods: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil–containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined. Results: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P < 0.001). Significantly lower (36–38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P < 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P < 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P < 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P < 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator–activated receptor α (Ppara), and peroxisome proliferator–activated receptor γ (Pparg) (P < 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident. Conclusions: Oil from transgenic Camelina is a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial

Overcoming myelosuppression due to synthetic lethal toxicity for FLT3-targeted acute myeloid leukemia therapy.

Activating mutations in FLT3 confer poor prognosis for individuals with acute myeloid leukemia (AML). Clinically active investigational FLT3 inhibitors can achieve complete remissions but their utility has been hampered by acquired resistance and myelosuppression attributed to a 'synthetic lethal toxicity' arising from simultaneous inhibition of FLT3 and KIT. We report a novel chemical strategy for selective FLT3 inhibition while avoiding KIT inhibition with the staurosporine analog, Star 27. Star 27 maintains potency against FLT3 in proliferation assays of FLT3-transformed cells compared with KIT-transformed cells, shows no toxicity towards normal human hematopoiesis at concentrations that inhibit primary FLT3-mutant AML blast growth, and is active against mutations that confer resistance to clinical inhibitors. As a more complete understanding of kinase networks emerges, it may be possible to define anti-targets such as KIT in the case of AML to allow improved kinase inhibitor design of clinical agents with enhanced efficacy and reduced toxicity

Treating Hematologic Malignancies During a Pandemic: Utilizing Telehealth and Digital Technology to Optimize Care.

In late January 2020, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2) was reported as an outbreak in Wuhan, China. Within 2 months it became a global pandemic. Patients with cancer are at highest risk for both contracting and suffering complications of its resultant disease, Coronavirus 19 (COVID-19). Healthcare systems across the world had to adapt quickly to mitigate this risk, while continuing to provide potentially lifesaving treatment to patients. Bringing care to the home through the use of telehealth, home based chemotherapy, and remote patient monitoring technologies can help minimize risk to the patient and healthcare workers without sacrificing quality of care delivered. These care models provide the right treatment, to the right patient, at the right time

Variational and Diffusion Quantum Monte Carlo Calculations with the CASINO Code

We present an overview of the variational and diffusion quantum Monte Carlo methods as implemented in the CASINO program. We particularly focus on developments made in the last decade, describing state-of-the-art quantum Monte Carlo algorithms and software and discussing their strengths and their weaknesses. We review a range of recent applications of CASINO