O pragmatismo como novo espaço de escrita do pós-moderno

O pragmatismo como novo espaço de escrita do pós-modern

Calibration of the dietary data obtained from the Brazilian center of the Natural History of HPV Infection in Men study: the HIM Study

O objetivo foi estimar as regressões de calibração dos dados dietéticos mensurados pelo questionário quantitativo de freqüência alimentar (QQFA) utilizado no Natural History of HPV Infection in Men: o Estudo HIM. Uma amostra de 98 indivíduos do estudo HIM respondeu, por meio de entrevista, a um QQFA e três recordatórios de 24 horas (R24h). A calibração foi feita por meio de análise de regressão linear, tendo os R24h como variável dependente e o QQFA como variável independente. Idade, índice de massa corporal, atividade física, renda e escolaridade foram utilizadas como variáveis de ajuste nos modelos. As médias geométricas dos R24h e do QQFA corrigido pela calibração são estatisticamente iguais. Os gráficos de dispersão entre os instrumentos demonstraram aumento da correlação após a correção dos dados, porém observa-se maior dispersão dos pontos de acordo com a piora do poder explicativo dos modelos. A identificação das regressões de calibração dos dados dietéticos do estudo HIM permitirá a estimativa do efeito da dieta sobre a infecção por HPV, corrigida pelo erro de medida do QQFAThe objective of this study was to estimate the regressions calibration for the dietary data that were measured using the quantitative food frequency questionnaire (QFFQ) in the Natural History of HPV Infection in Men: the HIM Study in Brazil. A sample of 98 individuals from the HIM study answered one QFFQ and three 24-hour recalls (24HR) at interviews. The calibration was performed using linear regression analysis in which the 24HR was the dependent variable and the QFFQ was the independent variable. Age, body mass index, physical activity, income and schooling were used as adjustment variables in the models. The geometric means between the 24HR and the calibration-corrected QFFQ were statistically equal. The dispersion graphs between the instruments demonstrate increased correlation after making the correction, although there is greater dispersion of the points with worse explanatory power of the models. Identification of the regressions calibration for the dietary data of the HIM study will make it possible to estimate the effect of the diet on HPV infection, corrected for the measurement error of the QFF

Development of a platform to align education and practice: bridging academia and the profession in Portugal

Limited fitness for practice may result from a mismatch between education and practice. Aiming to meet the common interests of academics and practitioners, the Portuguese Pharmaceutical Society (PPS) developed the Education and Practice Platform (EPP). The EPP includes one representative from each pharmacy faculty, and all Councils of Speciality Boards of Practice. Brainstorming with involved parties enabled sharing of interests, concerns and identifying a common path. Aims, mission, vision and values were set. The EPP's mission is to: act as an enabler to foster the quality and adequacy of education through sharing best practices, ultimately leading to facilitate professional integration, and to foster quality development in teaching practices with recognition for autonomy in freedom to teach and to learn. Its vision is an alignment of education and practice with the PPS' statutes to ensure validation of the competences defined for each practice area, and compliance with international guidance. Key performance indicators (KPIs) were set. Activities developed include the creation of a national forum to discuss education and practice, development of workshops on teaching methods and pharmacy internships, enhanced representation in international events and response to global and national requests. Ongoing work focuses on the creation of a common training framework in hospital and community pharmacy practice adapted to Portugal. The EPP is a worldwide case study, encouraging the development of discussion contributing to an open climate of sharing best practices, indirectly leading to foster a better alignment between education and practice. Many of these results are so far intangible in scientific terms but worth describing.info:eu-repo/semantics/publishedVersio

Crystal Structures of a Plant Trypsin Inhibitor from Enterolobium contortisiliquum (EcTI) and of Its Complex with Bovine Trypsin

A serine protease inhibitor from Enterolobium contortisiliquum (EcTI) belongs to the Kunitz family of plant inhibitors, common in plant seeds. It was shown that EcTI inhibits the invasion of gastric cancer cells through alterations in integrin-dependent cell signaling pathway. We determined high-resolution crystal structures of free EcTI (at 1.75 angstrom) and complexed with bovine trypsin (at 2 angstrom). High quality of the resulting electron density maps and the redundancy of structural information indicated that the sequence of the crystallized isoform contained 176 residues and differed from the one published previously. the structure of the complex confirmed the standard inhibitory mechanism in which the reactive loop of the inhibitor is docked into trypsin active site with the side chains of Arg64 and Ile65 occupying the S1 and S1' pockets, respectively. the overall conformation of the reactive loop undergoes only minor adjustments upon binding to trypsin. Larger deviations are seen in the vicinity of Arg64, driven by the needs to satisfy specificity requirements. A comparison of the EcTI-trypsin complex with the complexes of related Kunitz inhibitors has shown that rigid body rotation of the inhibitors by as much as 15 degrees is required for accurate juxtaposition of the reactive loop with the active site while preserving its conformation. Modeling of the putative complexes of EcTI with several serine proteases and a comparison with equivalent models for other Kunitz inhibitors elucidated the structural basis for the fine differences in their specificity, providing tools that might allow modification of their potency towards the individual enzymes.United States Department of Energy, Office of Science, Office of Basic Energy SciencesCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Institutes of Health, National Cancer Institute, Center for Cancer ResearchNCI, Ctr Canc Res, Macromol Crystallog Lab, Frederick, MD 21701 USAUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilInst Butantan, Lab Bioquim & Biofis, Unidade Sequenciamento Prot & Peptideos, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilUnited States Department of Energy, Office of Science, Office of Basic Energy Sciences: W-31-109-Eng-38FAPESP: 09/53766-5Web of Scienc

IKKβ kinase promotes stemness, migration, and invasion in KRAS-driven lung adenocarcinoma cells

KRAS oncogenic mutations are widespread in lung cancer and, because direct targeting of KRAS has proven to be challenging, KRAS-driven cancers lack effective therapies. One alternative strategy for developing KRAS targeted therapies is to identify downstream targets involved in promoting important malignant features, such as the acquisition of a cancer stem-like and metastatic phenotype. Based on previous studies showing that KRAS activates nuclear factor kappa-B (NF-κB) through inhibitor of nuclear factor kappa-B kinase β (IKKβ) to promote lung tumourigenesis, we hypothesized that inhibition of IKKβ would reduce stemness, migration and invasion of KRAS-mutant human lung cancer cells. We show that KRAS-driven lung tumoursphere-derived cells exhibit stemness features and increased IKKβ kinase activity. IKKβ targeting by different approaches reduces the expression of stemness-associated genes, tumoursphere formation, and self-renewal, and preferentially impairs the proliferation of KRAS-driven lung tumoursphere-derived cells. Moreover, we show that IKKβ targeting reduces tumour cell migration and invasion, potentially by regulating both expression and activity of matrix metalloproteinase 2 (MMP2). In conclusion, our results indicate that IKKβ is an important mediator of KRAS-induced stemness and invasive features in lung cancer, and, therefore, might constitute a promising strategy to lower recurrence rates, reduce metastatic dissemination, and improve survival of lung cancer patients with KRAS-driven disease

Resultados maternos y neonatales en un Centro de Parto Normal perihospitalario en la ciudad de São Paulo, Brasil

O objetivo foi descrever os resultados maternos e perinatais da assistência no Centro de Parto Normal Casa de Maria (CPN-CM), na cidade de São Paulo. A amostra probabilística foi de 991 parturientes e seus recém-nascidos, assistidos entre 2003 e 2006. Os resultados mostraram que 92,2% das parturientes tiveram um acompanhante de sua escolha e as práticas mais utilizadas no parto foram banho de aspersão ou imersão (92,9%), amniotomia (62,6%), deambulação (47,6%), massagem de conforto (29,8%) e episiotomia (25,7%). Com relação aos recém-nascidos, 99,9% apresentaram índice de Apgar = 7 no quinto minuto; 9,3% receberam aspiração das vias aéreas superiores; nenhum necessitou ser entubado; e 1,4% foram removidos para o hospital. O modelo de assistência praticado no CPN-CM apresenta resultados maternos e perinatais esperados para mulheres com baixo risco obstétrico, sendo alternativa segura e menos intervencionista no parto normal.The aim of this study was to describe the maternal and perinatal results of care in the alongside hospital birth center Casa de Maria (CPN-CM), located in the city of São Paulo. The random sample included 991 women and their newborns, attended between 2003 and 2006. The results showed that 92.2% of women had a companion of her choice during childbirth and the practices commonly used were shower or immersion bath (92.9%), amniotomy (62.6%), walking (47.6%), massage comfort (29.8%) and episiotomy (25.7%). Regarding newborns, 99.9% of them had Apgar scores =7 in the fifth minute, 9.3% received aspiration of the upper airway, no one needed to be intubated and 1.4% were removed to the hospital. The model of care in the CPN-CM provides maternal and perinatal outcomes expected for low obstetric risk women, and means a safe option and less interventionist model in normal childbirth.El objetivo fue describir los resultados de la atención materna y perinatal en el Centro de Parto Normal Casa de María (CPN-CM), en la ciudad de São Paulo, Brasil. La muestra probabilística se constituyó de 991 madres y sus recién nacidos, atendidos entre 2003 y 2006. Los resultados mostraron que 92,2% de las madres tenía un acompañante de su elección y las prácticas más utilizadas en el parto fueron el baño de aspersión o inmersión (92,9%), la amniotomía (62,6%), ambulación (47,6%), masaje de confortación (29,8%) y episiotomía (25,7%). Con respecto a los recién nacidos, el 99,9% presentaba índice de Apgar = 7 en el minuto cinco, el 9,3% recibió aspiración de las vías aéreas superiores, ninguno necesitó ser entubado y el 1,4% fue trasladado a un hospital. El modelo de atención practicado en el CPN-CN presenta resultados maternos y perinatales esperados para mujeres con bajo riesgo obstétrico, demostrando ser una alternativa segura y menos invasiva en el parto normal

Centros de Nacimiento en Brasil: revisión de la producción científica

Este artigo consiste em uma revisão narrativa com o objetivo de identificar a produção científica brasileira relacionada ao processo assistencial e aos resultados maternos e perinatais em centro de parto normal (CPN). As publicações foram recuperadas nas bases de dados e portais de periódicos PubMed/MEDLINE, CINAHAL, SciELO e REVENF. Incluíram-se, também, publicação em livro e produção não publicada de grupo de pesquisa. Foram selecionados oito estudos do tipo descritivo, dois transversais e dois casos-controle, realizados com 5.407 mulheres e 5.395 recém-nascidos, divulgados entre 2005 e 2009. Os estudos analisaram variáveis sócio-demográficas e obstétricas, práticas na assistência ao parto e nascimento e remoções maternas e neonatais para o hospital. A produção científica sobre CPN apresenta dados da última década, relativos a sete serviços. São, principalmente, estudos descritivos, com foco nas práticas obstétricas e nos resultados maternos, com ênfase menor na assistência neonatal.This article is a narrative review with the aim of identifying the Brazilian scientific production related to the care process and maternal and perinatal outcomes in birth centres (BC). The papers were recovered in the databases and portals PubMed/MEDLINE, CINAHAL, SciELO and REVENF. There were also included one book and the non published studies from a researcher group. There were selected eight descriptive, two cross-sectional and two case-control studies conducted with 5,407 women and 5,395 newborns, which were reported in the period from 2005 to 2009. These studies analyzed socio-demographic and obstetric outcomes in childbirth and maternal and neonatal transfers to the hospital setting. BC scientific production presents data about seven services, from the last decade. There are mainly descriptive studies, with focus on obstetric practices and maternal outcomes, with less emphasis on neonatal care.Este artículo es una revisión narrativa, con el objetivo de identificar la producción científica brasileña relacionada con la atención y los resultados maternos y perinatales en centros de nacimiento (CN). Las publicaciones fueron recuperadas en las bases de datos y portales PubMed/MEDLINE, CINAHAL, SciELO y REVENF. Fueron incluidos también libro publicado y los trabajos no publicados de un grupo de investigadores. Fueron seleccionados ocho estudios de tipo descriptivo, dos transversales y dos casos-control, realizados con 5.407 mujeres y 5.395 recién-nacidos, divulgados en el período de 2005 a 2009. Los estudios analizaron variables socio-demográficas y obstétricas, prácticas en la atención al parto y nacimiento y el traslado materno y neonatal hacia al hospital. La producción científica sobre CN presenta datos de la última década, relativos a siete servicios. Son estudios principalmente descriptivos, con enfoque en las prácticas obstétricas y en los resultados maternos, con menos énfasis en la atención neonatal

Análise físico-química de diferentes resíduos agroindustriais para possível utilização na indústria / Physico-chemical analysis of different agro-industrial residues for its use in the industry

A geração de resíduos na indústria alimentícia a partir do processamento de grãos, frutas e vegetais é muito comum e, em muitos casos, esses resíduos não possuem uma destinação apropriada. Com isso, este trabalho objetivou a avaliação da composição físico-química de diferentes resíduos da agroindústria, a fim de encontrar potencial para a reutilização dos mesmos. Assim, os materiais para este estudo foram: casca do maracujá, caroço de açaí, palha de arroz, casca do fruto do cacau e casca da semente do cacau, os quais foram submetidos às análises de umidade por infravermelho; teor de resíduo mineral fixo total (cinzas), lipídios totais, proteínas totais e pH, mensurados de acordo com o descrito pela AOAC. Com isso, pôde-se observar potenciais distintos em cada matéria-prima para o reaproveitamento dos mesmos, onde a casca do maracujá pode ser utilizada como suplemento alimentar, devido ao alto teor de fibras, descrito na literatura. O caroço do açaí pode ser utilizado na geração de energia, devido ao baixo teor de cinzas, bem como na suplementação alimentar de aves. A palha de arroz pode tanto auxiliar na produção de materiais como cimento e sílica, como na suplementação na ração animal.  A casca do fruto do cacau pode auxiliar na nutrição de solos e na dieta animal e a casca da semente do cacau pode ser utilizada na formulação de meios de cultivo para microrganismos e/ou tecidos vegetais