Projeto desenvolvido numa escola de um Território Educativo de Intervenção Prioritária

O presente relatório é o resultado do desenvolvimento de um projeto de Educação e Intervenção Social, com a metodologia de investigação-ação participativa e que teve como objetivo melhorar o relacionamento e as competências sociais dos alunos de uma turma com Percurso Curricular Alternativo. Este projeto em educação e intervenção social foi intitulado DesEtedietos do 9.ºE. Tratando-se de um projeto que tem por base a metodologia de investigação acima referida, o investigador e os participantes criaram uma relação necessária, para que mediante as necessidades encontradas, se tentasse proporcionar aos participantes desta investigação ferramentas que permitissem promover a melhoria dos relacionamentos interpessoais, bem como adquirir formas para ultrapassarem os seus problemas. Após uma cuidada análise do contexto, foram priorizados os problemas e necessidades e desenhadas três ações, duas com os alunos e uma com os pais. Este projeto teve como finalidade Desenvolver competências pessoais e sociais nos alunos da turma com Percurso Curricular Alternativo, com vista a uma integração positiva no seu trajeto académico e na sociedade. O projeto (DesEtedietos do 9.ºE revelou-se como positivo e significativo para os alunos e para os pais que nele participaram, permitindo uma constante reflexão e causando uma mudança nas suas atitudes e comportamentosThis report is the result of the development of a project of Education and Social Intervention with the participatory action research, methodology which aimed to improve relationships and social skills of students in a class with an Alternative Curriculum. This project in education and social intervention was en titled "(Un) understandings of 9.ºE". Since this is a project based on the abovementioned methodology, the researcher and the participants created a relationship in which, considering the needs that were found, tried to give participants tools that allowed to promote the improvement of interpersonal relationships, as well as acquire ways to overcome their problems. After careful analysis of the context, problems and needs were prioritized and were three actions, designed two with students and one with parents. This project aimed to "develop students personal and social skills, in the class with an Alternative Curriculum, to promote a positive integration in their academic path and in the society." The project "(Mis) Understandings of 9.ºE" turned out to be positive and significant for students and parents who participated in it, since it allowed a constant reflection, and a change in their attitudes and behavior

AS DIFICULDADES DA TRIAGEM DO ESPECTRO AUTISTA EM CRIANÇAS NA ATENÇÃO BÁSICA

The integrative review examines the role of family physicians and other primary care professionals in the identification and care of Autism Spectrum Disorder (ASD), emphasizing the importance of continuous education and interdisciplinary collaboration.To analyze the main challenges faced by healthcare professionals in conducting ASD screening in primary care, from the team's perspective. An integrative literature review was conducted, covering studies published from 2013 to 2023. The research was built based on the PICO (Patient, Intervention, Comparison, and Outcomes) strategy and used health descriptors related to ASD. 427 articles were identified, which underwent eligibility criteria. The selected articles were read in full, summarized, and the results were organized in a table.Ten studies were selected that addressed various aspects of ASD care and diagnosis. The results found pointed out the difficulties to be overcome by healthcare professionals so that they feel confident and capable of conducting early screening and diagnosis: the complexities of medical consultations and issues related to healthcare professionals' remuneration and the need for interdisciplinary collaboration.This integrative review identifies the complexity of ASD diagnosis and care and the need for an interdisciplinary and collaborative approach to improve the care of children on the spectrum, emphasizing the need to promote adequate training and support for primary care healthcare professionals to enhance the quality of life for children with ASD and their families.A revisão integrativa analisa o papel do médico de família e demais profissionais da atenção básica na identificação e cuidado do Transtorno do Espectro Autista (TEA), destacando a importância da formação contínua e colaboração interdisciplinar. Analisar as principais dificuldades enfrentadas pelos profissionais de saúde na condução da triagem do TEA na atenção básica, sob a perspectiva da equipe. Foi realizada uma revisão integrativa da literatura, abrangendo estudos publicados de 2013 a 2023. A pesquisa foi construída com base na estratégia PICO (Paciente, Intervenção, Comparação e Outcomes) e utilizou descritores em saúde relacionados ao TEA. Foram identificados 427 artigos, que passaram por critérios de elegibilidade. Os artigos selecionados foram lidos na íntegra, resumidos e os resultados organizados em um quadro. Foram selecionados dez estudos que abordaram facetas do cuidado e diagnóstico do TEA. Os resultados encontrados apontaram as dificuldades a serem superadas pelos profissionais da saúde, para que estes se sintam confiantes e capacitados a realizar a triagem e o diagnóstico de forma precoce: as complexidades das consultas médicas e questões relacionadas à remuneração dos profissionais de saúde e a necessidade de colaboração interdisciplinar. Esta revisão integrativa identificou a complexidade do diagnóstico e cuidado do TEA e a necessidade de uma abordagem interdisciplinar e colaborativa para melhorar o atendimento de crianças no espectro, enfatizando a necessidade de promover a capacitação e o suporte adequados aos profissionais de saúde na atenção primária para melhorar a qualidade de vida das crianças com TEA e suas famílias

LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3′-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions

LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.This work was supported by grants from the Spanish Ministerio de Educación y Ciencia (BFU2007-66933/BMC) and the German Research Foundation (DFG-FOR855). I.A.-R. and D.L. were supported by Fundaçao para a Ciência e Tecnología (SARH/BD/9630/2002; SFRH/BD/37047/2007

Soil organic matter and CO₂ fluxes in small tropical watersheds under forest and cacao agroforestry

<div><p>Annual estimates of CO₂ and dissolved carbon concentrations in the soil profile provide valuable insight into the dynamics of organic matter in soil and the effect of changes to vegetation cover. The aim of this study was to observe the spatial influence of litter decomposition in the first few centimeters of the soil for CO₂ fluxes and to describe the processing of soil organic matter throughout the soil profile by comparing three small tropical watersheds. Data were collected biweekly for six months, from December 2015 to May 2016. CO₂ was measured using an infrared gas analyzer in fixed chambers and the dissolved carbon of soil solution was analyzed in a TOC analyzer. No differences were found in the total soil CO₂ fluxes (control flux treatments) between the three study areas. In both cacao agroforestry systems (managed and unmanaged), total CO₂ fluxes were influenced by the decomposition of litter. CO₂ emissions in the soil profile of the cacao agroforestry systems were highly variable, compared to the preserved forest, and highly dependent on the soil characteristics attributed to the type of vegetation cover. Although a definite pattern between the temperature and soil moisture was not identified, these parameters showed a strong relationship in controlling the release of CO₂ between treatments. The organic and inorganic dissolved carbon patterns in the soil solution of the three areas revealed different responses of soil organic matter processing related to soil characteristics and vegetation. The results confirm the hypothesis that the top of soils (total CO₂ fluxes) of both cacao agroforestry systems (managed and unmanaged) emits fluxes of CO_2, which do not differ statistically from the preserved forest. However, depending on the soil characteristics, the cacao agroforestry system can result in an accumulation of CO₂ and dissolved inorganic carbon in the soil profile that is prone to being transported by hydrological routes to groundwater and stream water.</p></div

Principal component analysis (PCA) in monthly timescale determined by Axis 1 and Axis 2.

<p>(CO₂) CO₂ fluxes from the 40 cm treatment in mg CO2—C m² h^-1, (PREC) Precipitation in mm, (TEMP) temperature in °C, (MOISTURE) Soil moisture in %, (PF) Preserved forest, (MC) Managed cacao agroforestry system and (UC) Unmanaged cacao agroforestry system, (1, 2, 3, 4, 5 and 6) December, January, February, March, April and May, respectively.</p

Principal component analysis (PCA) in monthly timescale determined by Axis 1 and Axis 2.

<p>(CO₂) CO₂ fluxes from the 20 cm treatment in mg CO2—C m² h^-1, (PREC) Precipitation in mm, (TEMP) temperature in °C, (MOISTURE) Soil moisture in %, (PF) Preserved forest, (MC) Managed cacao agroforestry system and (UC) Unmanaged cacao agroforestry system, (1, 2, 3, 4, 5 and 6) December, January, February, March, April and May, respectively.</p

Location map of the small watersheds.

<p>(PF) Preserved forest, (MC) Managed cacao agroforestry system and (UC) Unmanaged cacao agroforestry system.</p