Biochemical properties of poplar thioredoxin z

AbstractTrx-z is a chloroplastic thioredoxin, exhibiting a usual WCGPC active site, but whose biochemical properties are unknown. We demonstrate here that Trx-z supports the activity of several plastidial antioxidant enzymes, such as thiol-peroxidases and methionine sulfoxide reductases, using electrons provided by ferredoxin–thioredoxin reductase. Its disulfide reductase activity requires the presence of both active site cysteines forming a catalytic disulfide bridge with a midpoint redox potential of −251mV at pH7. These in vitro biochemical data suggest that, besides its decisive role in the regulation of plastidial transcription, Trx-z might also be involved in stress response

Genomic Diversity of the Ostreid Herpesvirus Type 1 Across Time and Location and Among Host Species

The mechanisms underlying virus emergence are rarely well understood, making the appearance of outbreaks largely unpredictable. This is particularly true for pathogens with low per-site mutation rates, such as DNA viruses, that do not exhibit a large amount of evolutionary change among genetic sequences sampled at different time points. However, whole-genome sequencing can reveal the accumulation of novel genetic variation between samples, promising to render most, if not all, microbial pathogens measurably evolving and suitable for analytical techniques derived from population genetic theory. Here, we aim to assess the measurability of evolution on epidemiological time scales of the Ostreid herpesvirus 1 (OsHV-1), a double stranded DNA virus of which a new variant, OsHV-1 μVar, emerged in France in 2008, spreading across Europe and causing dramatic economic and ecological damage. We performed phylogenetic analyses of heterochronous (n = 21) OsHV-1 genomes sampled worldwide. Results show sufficient temporal signal in the viral sequences to proceed with phylogenetic molecular clock analyses and they indicate that the genetic diversity seen in these OsHV-1 isolates has arisen within the past three decades. OsHV-1 samples from France and New Zealand did not cluster together suggesting a spatial structuration of the viral populations. The genome-wide study of simple and complex polymorphisms shows that specific genomic regions are deleted in several isolates or accumulate a high number of substitutions. These contrasting and non-random patterns of polymorphism suggest that some genomic regions are affected by strong selective pressures. Interestingly, we also found variant genotypes within all infected individuals. Altogether, these results provide baseline evidence that whole genome sequencing could be used to study population dynamic processes of OsHV-1, and more broadly herpesviruses

What Does Ecological Farming Mean for Farm Labour?

Summary: Ecological farming, such as organic and low‐input farming, is gaining popularity in the public discourse. One question is how this type of farming may impact farm labour from a socio‐economic point of view. The article first discusses how low‐input farming practices (i.e. with lower reliance on inputs derived from fossil fuels) may affect the economic returns to labour, measured as the farm’s revenue per hour of labour input, on data from the Farm Accountancy Data Network (FADN) in 2004‐‐2015 for four European countries. Returns to labour appear to be highest at the two extremes – very low‐input farms and highly intensive farms. Farms in the low‐input end of the spectrum are in the minority, while the overwhelming majority of farms are intensive and have internal economic incentives to intensify further. The article also analyses how working conditions differ between organic and conventional dairy farms in two European countries based on interviews with farmers in 2019. Results show that all dimensions of working conditions are affected by being an organic farm or not, but this is not the only factor. There are many influences on working conditions, such as the production context and workforce composition

What Does Ecological Farming Mean for Farm Labour?

Summary: Ecological farming, such as organic and low‐input farming, is gaining popularity in the public discourse. One question is how this type of farming may impact farm labour from a socio‐economic point of view. The article first discusses how low‐input farming practices (i.e. with lower reliance on inputs derived from fossil fuels) may affect the economic returns to labour, measured as the farm’s revenue per hour of labour input, on data from the Farm Accountancy Data Network (FADN) in 2004‐‐2015 for four European countries. Returns to labour appear to be highest at the two extremes – very low‐input farms and highly intensive farms. Farms in the low‐input end of the spectrum are in the minority, while the overwhelming majority of farms are intensive and have internal economic incentives to intensify further. The article also analyses how working conditions differ between organic and conventional dairy farms in two European countries based on interviews with farmers in 2019. Results show that all dimensions of working conditions are affected by being an organic farm or not, but this is not the only factor. There are many influences on working conditions, such as the production context and workforce composition

The SIB Swiss Institute of Bioinformatics' resources: focus on curated databases

The SIB Swiss Institute of Bioinformatics (www.isb-sib.ch) provides world-class bioinformatics databases, software tools, services and training to the international life science community in academia and industry. These solutions allow life scientists to turn the exponentially growing amount of data into knowledge. Here, we provide an overview of SIB's resources and competence areas, with a strong focus on curated databases and SIB's most popular and widely used resources. In particular, SIB's Bioinformatics resource portal ExPASy features over 150 resources, including UniProtKB/Swiss-Prot, ENZYME, PROSITE, neXtProt, STRING, UniCarbKB, SugarBindDB, SwissRegulon, EPD, arrayMap, Bgee, SWISS-MODEL Repository, OMA, OrthoDB and other databases, which are briefly described in this article

Extracorporeal Membrane Oxygenation for Severe Acute Respiratory Distress Syndrome associated with COVID-19: An Emulated Target Trial Analysis.

RATIONALE: Whether COVID patients may benefit from extracorporeal membrane oxygenation (ECMO) compared with conventional invasive mechanical ventilation (IMV) remains unknown. OBJECTIVES: To estimate the effect of ECMO on 90-Day mortality vs IMV only Methods: Among 4,244 critically ill adult patients with COVID-19 included in a multicenter cohort study, we emulated a target trial comparing the treatment strategies of initiating ECMO vs. no ECMO within 7 days of IMV in patients with severe acute respiratory distress syndrome (PaO2/FiO2 <80 or PaCO2 ≥60 mmHg). We controlled for confounding using a multivariable Cox model based on predefined variables. MAIN RESULTS: 1,235 patients met the full eligibility criteria for the emulated trial, among whom 164 patients initiated ECMO. The ECMO strategy had a higher survival probability at Day-7 from the onset of eligibility criteria (87% vs 83%, risk difference: 4%, 95% CI 0;9%) which decreased during follow-up (survival at Day-90: 63% vs 65%, risk difference: -2%, 95% CI -10;5%). However, ECMO was associated with higher survival when performed in high-volume ECMO centers or in regions where a specific ECMO network organization was set up to handle high demand, and when initiated within the first 4 days of MV and in profoundly hypoxemic patients. CONCLUSIONS: In an emulated trial based on a nationwide COVID-19 cohort, we found differential survival over time of an ECMO compared with a no-ECMO strategy. However, ECMO was consistently associated with better outcomes when performed in high-volume centers and in regions with ECMO capacities specifically organized to handle high demand. This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/)

David Harvey, Brève Histoire du néolibéralisme, Paris, Les Prairies ordinaires, coll. « Penser/Croiser », 2014, 320 p.

International audienc

Recension. Matthieu de Nanteuil, Rendre justice au travail, Paris, PUF

International audienc

Bénédicte Vidaillet, Évaluez-moi ! Évaluation au travail : les ressorts d’une fascination, Paris, Le Seuil, 2013, 224 p.

Comment ne pas s’intéresser à l’évaluation, qui est partout et qui a gagné toutes les fonctions et tous les domaines d’activités en trente ans, si l’on s’accorde sur le constat liminaire de l’ouvrage de Bénédicte Vidaillet, psychanalyste et maître de conférences à l’université de Lille I ? Qualifiée d’« imposture », de « folie », de « tyrannie »…, l’évaluation a donné lieu récemment à de nombreuses critiques dénonçant l’usage qui en est fait dans les organisations contemporaines. Elle n’en co..

Introduction. La santé comme enjeu des relations professionnelles

Contributions rassemblées par le GRIS, Groupe de recherche innovations et société