1,208 research outputs found
The Jet and the Supernova in GRB990712
The optical light curve of the afterglow following the gamma-ray burst GRB990712 is re-examined. Recently published polarization measurements of that source require a collimated outflow geometry that in turn predicts a break in the light curve. We show that the V-band light curve is consistent with such a break and that the post-break light curve evolution is dominated by a supernova contribution
Polymer-coated bioactive glass S53P4 increases VEGF and TNF expression in an induced membrane model in vivo
The two-stage induced-membrane technique for treatment of large bone defects has become popular among orthopedic surgeons. In the first operation, the bone defect is filled with poly(methyl methacrylate) (PMMA), which is intended to produce a membrane around the implant. In the second operation, PMMA is replaced with autograft or allograft bone. Bioactive glasses (BAGs) are bone substitutes with bone-stimulating and angiogenetic properties. The aim of our study was to evaluate the inductive vascular capacity of BAG-S53P4 and poly(lactide-co-glycolide) (PLGA)-coated BAG-S53P4 for potential use as bone substitutes in a single-stage induced-membrane technique. Sintered porous rods of BAG-S53P4, PLGA-coated BAG-S53P4 and PMMA were implanted in the femur of 36 rabbits for 2, 4 and 8 weeks. The expression of vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF) in the induced membranes of implanted materials was analyzed with real-time quantitative polymerase chain reaction and compared with histology. Both uncoated BAG-S53P4 and PLGA-coated BAG-S53P4 increase expression of VEGF and TNF, resulting in higher amounts of capillary beds, compared with the lower expression of VEGF and less capillary beads observed for negative control and PMMA samples. A significantly higher expression of VEGF was observed for PLGA-coated BAG-S53P4 than for PMMA at 8 weeks (p <0.036). VEGF and TNF expression in the induced membrane of BAG-S53P4 and PLGA-coated BAG-S53P4 is equal or superior to PMMA, the "gold standard" material used in the induced-membrane technique. Furthermore, the VEGF and TNF expression for PLGA-coated BAG-S53P4 increased during follow-up.Peer reviewe
S53P4 bioactive glass scaffolds induce BMP expression and integrative bone formation in a critical-sized diaphysis defect treated with a single-staged induced membrane technique
Surgical management of critical-sized diaphyseal defects involves multiple challenges, and up to 10% result in delayed or non-union. The two-staged induced membrane technique is successfully used to treat these defects, but it is limited by the need of several procedures and bone graft. Repeated procedures increase costs and morbidity, while grafts are subject to donor-site complications and scarce availability. To transform this two-staged technique into one graft-independent procedure, we developed amorphous porous scaffolds sintered from the clinically used bioactive glass S53P4. This work constitutes the first evaluation of such scaffolds in vivo in a critical-sized diaphyseal defect in the weight-bearing rabbit femur. We provide important knowledge and prospects for future development of sintered S53P4 scaffolds as a bone substitute. Critical-sized diaphysis defects are complicated by inherent sub-optimal healing conditions. The two staged induced membrane technique has been used to treat these challenging defects since the 1980 & rsquo;s. It involves temporary implantation of a membrane-inducing spacer and subsequent bone graft defect filling. A single-staged, graft-independent technique would reduce both socio-economic costs and patient morbidity. Our aim was to enable such single-staged approach through development of a strong bioactive glass scaffold that could replace both the spacer and the graft filling. We constructed amorphous porous scaffolds of the clinically used bioactive glass S53P4 and evaluated them in vivo using a critical sized defect model in the weight-bearing femur diaphysis of New Zealand White rabbits. S53P4 scaffolds and standard polymethylmethacrylate spacers were implanted for 2, 4, and 8 weeks. Induced membranes were confirmed histologically, and their osteostimulative activity was evaluated through RT-qPCR of bone morphogenic protein 2, 4, and 7 (BMPs). Bone formation and osseointegration were examined using histology, scanning electron microscopy, energy-dispersive X-ray analysis, and micro-computed tomography imaging. Scaffold integration, defect union and osteosynthesis were assessed manually and with X-ray projections. We demonstrated that S53P4 scaffolds induce osteostimulative membranes and produce osseointegrative new bone formation throughout the scaffolds. We also demonstrated successful stable scaffold integration with early defect union at 8 weeks postoperative in critical-sized segmental diaphyseal defects with implanted sintered amorphous S53P4 scaffolds. This study presents important considerations for future research and the potential of the S53P4 bioactive glass as a bone substitute in large diaphyseal defects. Statement of significance Surgical management of critical-sized diaphyseal defects involves multiple challenges, and up to 10% result in delayed or non-union. The two-staged induced membrane technique is successfully used to treat these defects, but it is limited by the need of several procedures and bone graft. Repeated procedures increase costs and morbidity, while grafts are subject to donor-site complications and scarce availability. To transform this two-staged technique into one graft-independent procedure, we developed amorphous porous scaffolds sintered from the clinically used bioactive glass S53P4. This work constitutes the first evaluation of such scaffolds in vivo in a critical-sized diaphyseal defect in the weight-bearing rabbit femur. We provide important knowledge and prospects for future development of sintered S53P4 scaffolds as a bone substitute. (c) 2021 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )Peer reviewe
S53P4 bioactive glass scaffolds induce BMP expression and integrative bone formation in a critical-sized diaphysis defect treated with a single-stage d induce d membrane technique
Surgical management of critical-sized diaphyseal defects involves multiple challenges, and up to 10% result in delayed or non-union. The two-staged induced membrane technique is successfully used to treat these defects, but it is limited by the need of several procedures and bone graft. Repeated procedures increase costs and morbidity, while grafts are subject to donor-site complications and scarce availability. To transform this two-staged technique into one graft-independent procedure, we developed amorphous porous scaffolds sintered from the clinically used bioactive glass S53P4. This work constitutes the first evaluation of such scaffolds in vivo in a critical-sized diaphyseal defect in the weight-bearing rabbit femur. We provide important knowledge and prospects for future development of sintered S53P4 scaffolds as a bone substitute. Critical-sized diaphysis defects are complicated by inherent sub-optimal healing conditions. The two staged induced membrane technique has been used to treat these challenging defects since the 1980 & rsquo;s. It involves temporary implantation of a membrane-inducing spacer and subsequent bone graft defect filling. A single-staged, graft-independent technique would reduce both socio-economic costs and patient morbidity. Our aim was to enable such single-staged approach through development of a strong bioactive glass scaffold that could replace both the spacer and the graft filling. We constructed amorphous porous scaffolds of the clinically used bioactive glass S53P4 and evaluated them in vivo using a critical sized defect model in the weight-bearing femur diaphysis of New Zealand White rabbits. S53P4 scaffolds and standard polymethylmethacrylate spacers were implanted for 2, 4, and 8 weeks. Induced membranes were confirmed histologically, and their osteostimulative activity was evaluated through RT-qPCR of bone morphogenic protein 2, 4, and 7 (BMPs). Bone formation and osseointegration were examined using histology, scanning electron microscopy, energy-dispersive X-ray analysis, and micro-computed tomography imaging. Scaffold integration, defect union and osteosynthesis were assessed manually and with X-ray projections. We demonstrated that S53P4 scaffolds induce osteostimulative membranes and produce osseointegrative new bone formation throughout the scaffolds. We also demonstrated successful stable scaffold integration with early defect union at 8 weeks postoperative in critical-sized segmental diaphyseal defects with implanted sintered amorphous S53P4 scaffolds. This study presents important considerations for future research and the potential of the S53P4 bioactive glass as a bone substitute in large diaphyseal defects. Statement of significance Surgical management of critical-sized diaphyseal defects involves multiple challenges, and up to 10% result in delayed or non-union. The two-staged induced membrane technique is successfully used to treat these defects, but it is limited by the need of several procedures and bone graft. Repeated procedures increase costs and morbidity, while grafts are subject to donor-site complications and scarce availability. To transform this two-staged technique into one graft-independent procedure, we developed amorphous porous scaffolds sintered from the clinically used bioactive glass S53P4. This work constitutes the first evaluation of such scaffolds in vivo in a critical-sized diaphyseal defect in the weight-bearing rabbit femur. We provide important knowledge and prospects for future development of sintered S53P4 scaffolds as a bone substitute. (c) 2021 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )Peer reviewe
SAP30L interacts with members of the Sin3A corepressor complex and targets Sin3A to the nucleolus
Histone acetylation plays a key role in the regulation of gene expression. The chromatin structure and accessibility of genes to transcription factors is regulated by enzymes that acetylate and deacetylate histones. The Sin3A corepressor complex recruits histone deacetylases and in many cases represses transcription. Here, we report that SAP30L, a close homolog of Sin3-associated protein 30 (SAP30), interacts with several components of the Sin3A corepressor complex. We show that it binds to the PAH3/HID (Paired Amphipathic Helix 3/Histone deacetylase Interacting Domain) region of mouse Sin3A with residues 120–140 in the C-terminal part of the protein. We provide evidence that SAP30L induces transcriptional repression, possibly via recruitment of Sin3A and histone deacetylases. Finally, we characterize a functional nucleolar localization signal in SAP30L and show that SAP30L and SAP30 are able to target Sin3A to the nucleolus
Precursor flares in OJ 287
We have studied three most recent precursor flares in the light curve of the
blazar OJ 287 while invoking the presence of a precessing binary black hole in
the system to explain the nature of these flares. Precursor flare timings from
the historical light curves are compared with theoretical predictions from our
model that incorporate effects of an accretion disk and post-Newtonian
description for the binary black hole orbit. We find that the precursor flares
coincide with the secondary black hole descending towards the accretion disk of
the primary black hole from the observed side, with a mean z-component of
approximately z_c = 4000 AU. We use this model of precursor flares to predict
that precursor flare of similar nature should happen around 2020.96 before the
next major outburst in 2022.Comment: to appear in the Astrophysical Journa
AGILE observation of a gamma-ray flare from the blazar 3C 279
Context. We report the detection by the AGILE satellite of an intense
gamma-ray flare from the gamma-ray source 3EG J1255-0549, associated to the
Flat Spectrum Radio Quasar 3C 279, during the AGILE pointings towards the Virgo
Region on 2007 July 9-13.
Aims. The simultaneous optical, X-ray and gamma-ray covering allows us to
study the spectral energy distribution (SED) and the theoretical models
relative to the flaring episode of mid-July.
Methods. AGILE observed the source during its Science Performance
Verification Phase with its two co-aligned imagers: the Gamma- Ray Imaging
Detector (GRID) and the hard X-ray imager (Super-AGILE) sensitive in the 30 MeV
- 50 GeV and 18 - 60 keV respectively. During the AGILE observation the source
was monitored simultaneously in optical band by the REM telescope and in the
X-ray band by the Swift satellite through 4 ToO observations.
Results. During 2007 July 9-13 July 2007, AGILE-GRID detected gamma-ray
emission from 3C 279, with the source at ~2 deg from the center of the Field of
View, with an average flux of (210+-38) 10^-8 ph cm^-2 s^-1 for energy above
100 MeV. No emission was detected by Super-AGILE, with a 3-sigma upper limit of
10 mCrab. During the observation lasted about 4 days no significative gamma-ray
flux variation was observed.
Conclusions. The Spectral Energy Distribution is modelled with a homogeneous
one-zone Synchrotron Self Compton emission plus the contributions by external
Compton scattering of direct disk radiation and, to a lesser extent, by
external Compton scattering of photons from the Broad Line Region.Comment: Accepted for publication in Astronomy and Astrophysic
Another look at the BL Lacertae flux and spectral variability
The GLAST-AGILE Support Program (GASP) of the Whole Earth Blazar Telescope
(WEBT) monitored BL Lacertae in 2008-2009 at radio, near-IR, and optical
frequencies. During this period, high-energy observations were performed by
XMM-Newton, Swift, and Fermi. We analyse these data with particular attention
to the calibration of Swift UV data, and apply a helical jet model to interpret
the source broad-band variability. The GASP-WEBT observations show an optical
flare in 2008 February-March, and oscillations of several tenths of mag on a
few-day time scale afterwards. The radio flux is only mildly variable. The UV
data from both XMM-Newton and Swift seem to confirm a UV excess that is likely
caused by thermal emission from the accretion disc. The X-ray data from
XMM-Newton indicate a strongly concave spectrum, as well as moderate flux
variability on an hour time scale. The Swift X-ray data reveal fast (interday)
flux changes, not correlated with those observed at lower energies. We compare
the spectral energy distribution (SED) corresponding to the 2008 low-brightness
state, which was characterised by a synchrotron dominance, to the 1997 outburst
state, where the inverse-Compton emission was prevailing. A fit with an
inhomogeneous helical jet model suggests that two synchrotron components are at
work with their self inverse-Compton emission. Most likely, they represent the
radiation from two distinct emitting regions in the jet. We show that the
difference between the source SEDs in 2008 and 1997 can be explained in terms
of pure geometrical variations. The outburst state occurred when the
jet-emitting regions were better aligned with the line of sight, producing an
increase of the Doppler beaming factor. Our analysis demonstrates that the jet
geometry can play an extremely important role in the BL Lacertae flux and
spectral variability.Comment: 12 pages, 10 figures, accepted for publication in A&
INTEGRAL observations of the field of the BL Lacertae object S5~0716+714
We have performed observations of the blazar S5 0716+714 with INTEGRAL on 2-6
April 2004. In the first months of 2004, the source had increased steadily in
optical brightness and had undergone two outbursts. During the latter, occurred
in March, it reached the extreme level of R = 12.1 mag, which triggered our
INTEGRAL program. The target has been detected with IBIS/ISGRI up to 60 keV,
with a flux of ~3 x 10e-11 erg/s/cm2 in the 30-60 keV interval, a factor of ~2
higher than observed by the BeppoSAX PDS in October 2000. In the field of S5
0716+714 we have also detected the Flat Spectrum Radio Quasar S5 0836+710 and
the two Seyfert galaxies Mkn 3 and Mkn 6. Their IBIS/ISGRI spectra are rather
flat, albeit consistent with those measured by BeppoSAX. In the spectrum of Mkn
3 we find some evidence of a break between ~60 and ~100 keV, reminiscent of the
high energy cut-offs observed in other Seyfert galaxies. This is the first
report of INTEGRAL spectra of weak Active Galactic Nuclei.Comment: 5 pages, 5 figures, in press in A&
The 72-Hour WEBT Microvariability Observation of Blazar S5 0716+714 in 2009
Context. The international whole earth blazar telescope (WEBT) consortium
planned and carried out three days of intensive micro-variability observations
of S5 0716+714 from February 22, 2009 to February 25, 2009. This object was
chosen due to its bright apparent magnitude range, its high declination, and
its very large duty cycle for micro-variations. Aims. We report here on the
long continuous optical micro-variability light curve of 0716+714 obtained
during the multi-site observing campaign during which the Blazar showed almost
constant variability over a 0.5 magnitude range. The resulting light curve is
presented here for the first time. Observations from participating
observatories were corrected for instrumental differences and combined to
construct the overall smoothed light curve. Methods. Thirty-six observatories
in sixteen countries participated in this continuous monitoring program and
twenty of them submitted data for compilation into a continuous light curve.
The light curve was analyzed using several techniques including Fourier
transform, Wavelet and noise analysis techniques. Those results led us to model
the light curve by attributing the variations to a series of synchrotron
pulses. Results. We have interpreted the observed microvariations in this
extended light curve in terms of a new model consisting of individual
stochastic pulses due to cells in a turbulent jet which are energized by a
passing shock and cool by means of synchrotron emission. We obtained an
excellent fit to the 72-hour light curve with the synchrotron pulse model
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