Observing mesoscale eddy effects on mode-water subduction and transport in the North Pacific.

While modelling studies suggest that mesoscale eddies strengthen the subduction of mode waters, this eddy effect has never been observed in the field. Here we report results from a field campaign from March 2014 that captured the eddy effects on mode-water subduction south of the Kuroshio Extension east of Japan. The experiment deployed 17 Argo floats in an anticyclonic eddy (AC) with enhanced daily sampling. Analysis of over 3,000 hydrographic profiles following the AC reveals that potential vorticity and apparent oxygen utilization distributions are asymmetric outside the AC core, with enhanced subduction near the southeastern rim of the AC. There, the southward eddy flow advects newly ventilated mode water from the north into the main thermocline. Our results show that subduction by eddy lateral advection is comparable in magnitude to that by the mean flow--an effect that needs to be better represented in climate models

Prediction of Dynamic Mechanical Behaviors of Coral Concrete under Different Corrosive Environments and its Enhancement Mechanism

The marine structures are usually exposed to dynamic loadings within short periods and chemical attacks, resulting in severe dynamical damage or failure to concrete structures. The study of the time-dependent changes in the dynamic mechanical behavior of coral concrete under different corrosive environments and low-frequency waves is limited. For the safety and reliable use of coral concrete in the marine environment, different corrosion environments (chloride ions, sulfate ions, and mixed chloride-sulfate ions) and low-frequency waves (0.5–2.0 Hz) were designed to evaluate the dynamic properties of concrete. A model considering the effect of corroded age is formulated to predict the damping capacity of coral concrete. Testing results indicate that sulfate attack shows the most significant effect to influence the dynamic behaviors of coral concrete, while the effect of chloride ion penetration is negligible. The loss factor of coral concrete under corroded environments increases by 59.5% compared with ordinary concrete, even though the loss modulus and storage modulus of coral concrete reduce by 38.5% and 51.8%. It was attributed to coral concrete showing a low ability to resist sulfate attack, resulting in more cracks and pores in the matrix. Coral aggregate with high porosity and interconnected pores in coral concrete works like a cushion to dissipate more external energy. The proposed prediction model (R2 = 0.89) can accurately describe the relationship between erosion age and damping capacity in different corrosion environments, which can guide the application of coral concrete in marine and vibration environments

Effect of different rice planting methods on the water, energy and carbon footprints of subsequent wheat

The rice-wheat rotation system is an important planting system in the middle and lower reaches of the Yangtze River. Studies on the effects of different rice planting methods on the water, energy, and carbon footprints of subsequent wheat have rarely been reported. In this study, the effects of different rice cultivation practices on the water, energy, and carbon footprints of subsequent wheat were investigated among different rice-wheat rotation systems including dry direct-seeded rice (DSR)-wheat rotation, wet direct-seeded rice (WSR)-wheat rotation and transplanted rice(TPR)-wheat rotation. Results showed that the yield of wheat after DSR was 8,552 kg ha−1, which was 14.61 and 4.72% higher than the yields after WSR and TPR, respectively. In addition, the water and carbon footprints of wheat after DSR were lower than those after WSR and TPR, while its energy and carbon production efficiencies and net ecosystem economic benefits were higher than those after WSR and TPR. Notably, the use of fertilizers and fuel are the two major contributors to the high energy inputs and greenhouse gas emissions in wheat production. In summary, wheat after DSR has higher ecological and economic benefits, and we recommend that it be promoted as the preferred wheat planting model in rice-wheat rotation areas

Rice yield penalty and quality deterioration is associated with failure of nitrogen uptake from regreening to panicle initiation stage under salinity

In recent years, the development and utilization of saline land for rice cultivation have effectively expanded grain productivity. Rice is a salt-sensitive crop, and the increasing salinity problem threatens rice yield and quality. Therefore, we conducted open field experiments to study the effect of salinity on different growth stages of rice. Irrigating saline treatment was conducted at three different growth stages: irrigating saline from the regreening stage to the panicle initiation stage (S1), irrigating saline from the panicle initiation stage to the flowering stage (S2), and irrigating saline from the flowering stage to the maturity stage (S3). Each treatment period lasted for about 30 days. At the same time, irrigating saline water from the regreening stage to the maturity stage (S4) treatment was added in 2022 to explore the performance of salt stress during the whole growth period of rice. Based on the treatment of these different saline irrigation growth periods, three saline concentrations were incorporated, including salinity 0‰ (T1), 3‰ (T2), and 6‰ (T3) concentrations. No irrigating saline during the whole growth period was also used as a control (CK). The results indicated that rice grain yield and quality were most sensitive to saline treatment during S1 among the three stress periods. At the S1 stage, salinity mainly reduced the nitrogen uptake, resulting in stunted plant growth, reducing tillering, yield, and yield components, and deteriorating the rice quality. Compared to the control, IEN (grain yield over the total amount of N uptake in plants at maturity) was more sensitive at the S1 stage than S2 and S3 stages under salinity. Furthermore, the findings of our study suggest that under salinity, rice growth is not only directly affected by the higher sodium (Na+) content in plants, but the higher concentration of Na+ reduced the ability of plants to uptake nitrogen. Thus, more attention should be paid to the field management of the S1 stage, the most sensitive stage during rice cultivation in salinized areas. It is necessary to avoid salt damage to rice during this period and ensure irrigation with precious freshwater resources

Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells.

MicroRNAs (miRNAs) loss-of-function phenotypes are mainly induced by chemically modified antisense oligonucleotides. Here we develop an alternative inhibitor for miRNAs, termed \u27small RNA zipper\u27. It is designed to connect miRNA molecules end to end, forming a DNA-RNA duplex through a complementary interaction with high affinity, high specificity and high stability. Two miRNAs, miR-221 and miR-17, are tested in human breast cancer cell lines, demonstrating the 70∼90% knockdown of miRNA levels by 30-50 nM small RNA zippers. The miR-221 zipper shows capability in rescuing the expression of target genes of miR-221 and reversing the oncogenic function of miR-221 in breast cancer cells. In addition, we demonstrate that the miR-221 zipper attenuates doxorubicin resistance with higher efficiency than anti-miR-221 in human breast cancer cells. Taken together, small RNA zippers are a miRNA inhibitor, which can be used to induce miRNA loss-of-function phenotypes and validate miRNA target genes

Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker)

Olfaction in insects has a critical role in recognizing the host, finding food, and choosing mating partners, as well as avoiding predators. Odorant receptors (ORs), which are housed in the dendritic membrane of sensory neurons and extended into the lymph of sensilla on insect antennae, are participating in the detection of volatile compounds in insects. In the present study, we identified an OR gene, named MsepOR13, in the oriental armyworm Mythimna separata (Walker). Quantitative real-time polymerase chain reaction revealed that MsepOR13 was expressed mainly in the antennae of male and female moths. In in vitro heterologous expression experiments, MsepOR13 was widely tuned to 32 of the 67 different compounds tested. Furthermore, MsepOR13 responded to eugenol at a low concentration of 10-9 M, with an EC50 value of 3.91 × 10-6 M. The high sensitivity suggests an important role for the OR13 gene in the moth olfactory system

Solutions for recycling emerging wind turbine blade waste in China are not yet effective

Wind power supply chains are evolving as markets expand to reach climate goals. With the largest installed wind power capacity globally, China must deal with increasing composite turbine waste and anticipate its associated costs. Here we predict the quantity and composition of wind turbine blade waste based on historic deployment. A high-resolution database containing 14 turbine capacities (150–5500 kilowatts) was compiled based on 104 turbine models. The environmental and financial costs of waste treatment options were evaluated using a bottom-up approach. Based on current installations and future projections, 7.7 to 23.1 million tonnes of blade waste will be generated in China by 2050. Technologies exist to recycle glass fibre from blade waste, but these solutions vary in level of maturity and are not always commercially available, cost-competitive, or environmentally sustainable. Our findings can inform decision-makers in governments and industry on the pathways to carbon neutrality

Genetic Diagnostic Evaluation of Trio-Based Whole Exome Sequencing Among Children With Diagnosed or Suspected Autism Spectrum Disorder

Autism spectrum disorder (ASD) is a group of clinically and genetically heterogeneous neurodevelopmental disorders. Recent tremendous advances in the whole exome sequencing (WES) enable rapid identification of variants associated with ASD including single nucleotide variations (SNVs) and indels. To further explore genetic etiology of ASD in Chinese children with negative findings of copy number variants (CNVs), we applied WES in 80 simplex families with a single affected offspring with ASD or suspected ASD, and validated variations predicted to be damaging by Sanger sequencing. The results showed that an overall diagnostic yield of 8.8% (9.2% in the group of ASD and 6.7% in the group of suspected ASD) was observed in our cohort. Among patients with diagnosed ASD, developmental delay or intellectual disability (DD/ID) was the most common comorbidity with a diagnostic yield of 13.3%, followed by seizures (50.0%) and craniofacial anomalies (40.0%). All of identified de novo SNVs and indels among patients with ASD were loss of function (LOF) variations and were slightly more frequent among female (male vs. female: 7.3% vs. 8.5%). A total of seven presumed causative genes (CHD8, AFF2, ADNP, POGZ, SHANK3, IL1RAPL1, and PTEN) were identified in this study. In conclusion, WES is an efficient diagnostic tool for diagnosed ASD especially those with negative findings of CNVs and other neurological disorders in clinical practice, enabling early identification of disease related genes and contributing to precision and personalized medicine

Evaluation of nitrogen balance in a direct-seeded-rice field experiment using Hydrus-1D

Nitrogen (N) pollution is a global environmental problem that has greatly increased the risks of both the eutrophication of surface waters and contamination of ground waters. The majority of N pollution mainly comes from agricultural fields, in particular during rice growing seasons. In recent years, a gradual shift from the transplanting rice cultivation method to the direct seeding method has occurred, which results in different water and N losses from paddy fields and leads to distinct impacts on water environments. The N transport and transformations in an experimental direct-seeded-rice (DSR) field in the Taihu Lake Basin of east China were observed during two consecutive seasons, and simulated using Hydrus-1D model. The observed crop N uptake, ammonia volatilization (AV), N concentrations in soil, and N leaching were used to calibrate and validate the model parameters. The two most important inputs of N, i.e., fertilization and mineralization, were considered in the simulations with 220 and 145.5kgha-1 in 2008 and 220 and 147.8kgha-1 in 2009, respectively. Ammonia volatilization and nitrate denitrification were the two dominant pathways of N loss, accounting for about 16.0% and 38.8% of the total N input (TNI), respectively. Both nitrification and denitrification processes mainly occurred in the root zone. N leaching at 60 and 120cm depths accounted for about 6.8% and 2.7% of TNI, respectively. The crop N uptake was 32.1% and 30.8% of TNI during the 2008 and 2009 seasons, respectively, and ammonium was the predominant form (74% of the total N uptake on average). Simulated N concentrations and fluxes in soil matched well with the corresponding observed data. Hydrus-1D could simulate the N transport and transformations in the DSR field, and could thus be a good tool for designing optimal fertilizer management practices in the future