Molecular characterization and expression analysis of a hepcidin gene from rice field eel (Monopterus albus)

Hepcidin is a cysteine-rich, dual-function peptide with antimicrobial activity that plays crucial roles in iron homeostasis. A few hepcidin-like genes have been isolated from teleost. Here, we have identified a hepcidin-like gene from rice field eel (RFE), Monopterus albus. Nucleotide sequences including cDNA and genomic DNA (GenBank accession numbers: FJ436808 and FJ594996, respectively) and deduced amino acid sequences were presented. In the 949 bp-long genomic sequence, two introns and three exons were identified. The full-length cDNA encodes a prepropeptide of 90 amino acid residues. RTPCR analysis suggested that hepcidin transcripts are highly abundant in the liver and kidney, less abundant in the heart, skin, brain, blood cells, intestine, spleen and stomach and undetectable in muscle. After challenged with Aeromonas hydrophila infection or iron-dextran stimulation, the hepcidin transcript levels were analyzed by RT-PCR. The results revealed that the expression of hepcidin dramatically increased at 24 h post-infection of the pathogen injection. Moreover, hepcidin mRNAs in the liver, intestine and brain were 2.4, 1.5 and 2-fold increase, respectively, compared with the control animals after 5 days in iron-dextran injected RFEs.Key words: Rice field eel, Monopterus albus, hepcidin, gene expressio

Effects of sputtering and annealing temperatures on MOS capacitor with HfTiON gate dielectric

In this work, Al/HfTiON/n-Si capacitors with different sputtering and annealing temperatures are studied. Larger accumulation capacitance and flat-band voltage are observed for samples with higher sputtering or post-deposition annealing temperature. Gate conduction mechanisms are only affected by sputtering temperature slightly. The flat-band voltage shift and interface-state density at midgap under high-field gate injection and substrate injection are investigated, and the results imply electron detrapping in the gate dielectric. ©2009 IEEE.published_or_final_versionThe IEEE International Conference of Electron Devices and Solid-State Circuits (EDSSC 2009), Xi'an, China, 25-27 December 2009. In Proceedings of EDSSC, 2009, p. 209-21

Ultrasonic extraction of flavonoids and phenolics from loquat (Eriobotrya japonica Lindl.) flowers

Ethanol was used to extract flavonoids and phenolics from loquat (Eriobotrya japonica Lindl. cv. Ruantiaobaisha) flowers with ultrasonic pharmaceutical managing machine. Single-factor and orthogonal experiment were used to investigate the optimum extraction condition. The results showed that, the combination of 30°C, 80 min, 60% ethanol and 1:40 material ratio was optimum extraction condition with the highest yields of flavonoids and phenolics at 47 kHz/500 W. Under the optimum extraction condition, two consecutive extractions was enough, the extraction rates of flavonoids and phenolics were all more than 90%, with the contents of 10.59 and 102.02 mg/g dry weight, respectively.Key words: Eriobotrya japonica, flower, flavonoids, phenolics, ultrasonic extraction

Post-stress interface trap generation induced by oxide-field stress with FN injection

Interface trap generation in nMOS transistors during both stressing and post-stress periods under the conditions of oxide field (dynamic and dc) stress with FN injection is investigated with charge pumping technique. In contrast to the post-stress interface trap generation induced by hot carrier stress which is a logarithmical function of post-stress time, the poststress interface trap generation induced by oxide-field stress with FN injection first increases with post-stress time but then becomes saturated. The mechanisms for the interface trap generation in both stressing and post-stress periods are described. © 1998 IEEE.published_or_final_versio

Accelerating exhaustive pairwise metagenomic comparisons

In this manuscript, we present an optimized and parallel version of our previous work IMSAME, an exhaustive gapped aligner for the pairwise and accurate comparison of metagenomes. Parallelization strategies are applied to take advantage of modern multiprocessor architectures. In addition, sequential optimizations in CPU time and memory consumption are provided. These algorithmic and computational enhancements enable IMSAME to calculate near optimal alignments which are used to directly assess similarity between metagenomes without requiring reference databases. We show that the overall efficiency of the parallel implementation is superior to 80% while retaining scalability as the number of parallel cores used increases. Moreover, we also show thats equential optimizations yield up to 8x speedup for scenarios with larger data.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Computable randomness is about more than probabilities

We introduce a notion of computable randomness for infinite sequences that generalises the classical version in two important ways. First, our definition of computable randomness is associated with imprecise probability models, in the sense that we consider lower expectations (or sets of probabilities) instead of classical 'precise' probabilities. Secondly, instead of binary sequences, we consider sequences whose elements take values in some finite sample space. Interestingly, we find that every sequence is computably random with respect to at least one lower expectation, and that lower expectations that are more informative have fewer computably random sequences. This leads to the intriguing question whether every sequence is computably random with respect to a unique most informative lower expectation. We study this question in some detail and provide a partial answer

The effect of thermal annealing on the properties of indium tin oxide thin films

ITO thin films were deposited on glass substrates using e-beam evaporation. The influence of post-deposition annealing on the optical properties of the films was investigated in detail. It was found that the annealing conditions strongly affect the optical properties of the films. The transmittance of films annealed in forming gas (mixed 80% N 2 and H 2) at first increases dramatically with increasing annealing temperatures up to 300°C but then drops for higher temperature anneals around 400°C. An interesting phenomenon is that the transmittance of the darkened film can recover under further 400°C annealing in air. Atomic force microscopy, X-ray diffraction and X-ray photoemission spectroscopy have been employed to obtain information on the chemical state and crystallization of the films. Analysis of this data suggests that incorporation and decomposition reactions of oxygen can be controlled to reversibly change the optical properties of the ITO thin film. © 2005 IEEE.published_or_final_versio

Influence of gaseous annealing environment on the properties of indium-tin-oxide thin films

The influence of postannealing in different gaseous environments on the optical properties of indiu-tin-oxide (ITO) thin films deposited on glass substrates using e-beam evaporation has been systematically investigated. It is found that the annealing conditions affect the optical and electrical properties of the films. Atomic force microscopy, x-ray diffraction, and x-ray photoemission spectroscopy (XPS) were employed to obtain information on the chemical state and crystallization of the films. These data suggest that the chemical states and surface morphology of the ITO film are strongly influenced by the gaseous environment during the annealing process. The XPS data indicate that the observed variations in the optical transmittance can be explained by oxygen incorporation into the film, decomposition of the indium oxide phases, as well as the removal of metallic In. © 2005 American Institute of Physics.published_or_final_versio

Seeing two faces together: preference formation in humans and rhesus macaques

Humans, great apes and old world monkeys show selective attention to faces depending on conspecificity, familiarity, and social status supporting the view that primates share similar face processing mechanisms. Although many studies have been done on face scanning strategy in monkeys and humans, the mechanisms influencing viewing preference have received little attention. To determine how face categories influence viewing preference in humans and rhesus macaques (Macaca mulatta), we performed two eye-tracking experiments using a visual preference task whereby pairs of faces from different species were presented simultaneously. The results indicated that viewing time was significantly influenced by the pairing of the face categories. Humans showed a strong bias towards an own-race face in an Asian–Caucasian condition. Rhesus macaques directed more attention towards non-human primate faces when they were paired with human faces, regardless of the species. When rhesus faces were paired with faces from Barbary macaques (Macaca sylvanus) or chimpanzees (Pan troglodytes), the novel species’ faces attracted more attention. These results indicate that monkeys’ viewing preferences, as assessed by a visual preference task, are modulated by several factors, species and dominance being the most influential