Relato de Atividade: “Faço Parte Dessa História”

The present work consists in the report of an activity carried out with students in their first semester of the Nutrition course of the Universidade de São Paulo in 2014. This activity had as the proposal the construction of a timeline by students in the classroom about the history of the profession of nutritionist in Brazil. The aim of this report is to critically analyze the use of active teaching methodologies in higher education in order to transform the student from passive to active member in the classroom dynamics. The activity obtained good results by the view of the students who reported better use and dedication to the content with the dynamics used.O presente trabalho consiste no relato de uma atividade realizada com alunos do primeiro semestre do curso de Nutrição da Universidade de São Paulo em 2014. Tal atividade teve como proposta a construção pelos alunos, em sala de aula, de uma linha do tempo sobre a história da profissão do nutricionista no Brasil. O objetivo do relato é analisar criticamente o uso de metodologias ativas no ensino superior visando à transformação do aluno de membro passivo em membro ativo na dinâmica da sala de aula. A atividade realizada obteve bons resultados, segundo a visão dos alunos, que relataram ter havido melhor aproveitamento e dedicação ao conteúdo com a dinâmica utilizada

A retrospective study of canine cutaneous mast cell tumor: correlation between clinical, histological and molecular characteristics/ Um estudo retrospectivo de mastócitos cutâneos caninos: correlação entre características clínicas, histológicas e moleculares

Mast cell tumor (MCT), or mastocytoma, the second most common skin tumor in dogs, is characterized by neoplastic transformations and abnormal proliferation of mast cells from cutaneous or visceral origins. Mutations in the proto-oncogene c-kit are present in 15% of dogs with MCT and up to 35% in high-grade tumors. In the clinical management of the disease, some characteristics, such as anatomical occurrence, histological grading, clinical staging, age and breed, are important parameters to better drive the treatment and predict the prognosis of each patient. However, it is not clear the correlations between c-kit mutation and clinical/epidemiological data, demonstrating the necessity of new studies. In this study, 78 clinical cases of canine patients with MCT were analyzed in Campinas - SP, Brazil, at Oncovida - Veterinary Oncology Center, in the period of 2017 to 2019, to determine the frequency, prevalence and correlations between molecular, clinical and epidemiological aspects. Results presented here shows that mixed breed dogs (51%) were the most affected, followed by Labrador (17%) and Boxer (16%). Most dogs were female (67%), aged 6 to 10 years (62%); 42% of the animals had tumors located in the pelvic limb and 17% had nodules in the chest. The most used method for diagnosis was histopathology, totalizing 60% of cases, followed by aspirative cytology (26%) and immunohistochemistry (8%). Regarding histological variables, 3 patients had grade I (11.8%), 47 had grade II (61%) and 15 had grade III (19%) and, according to KIUPEL et al. (2011) classification, 77 % of the cases presented low grade and 23% were high grade. The survival of these patients was also analyzed, demonstrating that animals presenting grade I and grade II (low grade) had longer survival (68%) in relation to grade III (high grade) (16%) The variables showed a significant correlation between histological grade and c-kit mutation with breed, age, localization of the tumor, diagnostic, chosen treatment and survival, indicating that they are both decisive to the outcome of the disease. Only sex was not correlated, suggesting that both the grade of the tumor and the c-kit mutation were not found to contribute to a different development of the tumor in male or female. These results demonstrate important data on the clinical behavior of MCT in the region of Campinas-SP, presenting relevant knowledge that can be used in the future in the clinical manage of the patients with this disease and demonstrating the necessity of further studies on the epidemiology of MCT in each region of Brazil

Tradition, Acculturation and Authenticity in tourist reports about indigenous peoples in a virtual environment

Atraves da analise de todas as terras indigenas brasileiras cadastradas no referido aplicativo, se busca investigar quais conceitos e categorias sao colocados em jogo pelos atores sociais para se referir e analisar as Terras Indigenas brasileiras como atrativos turisticos. Tambem e objetivo importante analisar o modo como turistas se referem aos povos indigenas em suas publicacoes, bem como a construcao de estereótipos e modos de pensar a respeito da indianidade. Acredita‑se que tal analise aporta importantes contribuicoes para o contexto cientifico de pesquisa em turismo. Tanto por utilizar meios relativamente novos para a realização de pesquisa, quanto por abordar tema tambem de literatura relativamente pouco desenvolvida na area, isto e, turismo em Terras Indigenas. Outro dado relevante se refere ao fato de que os comentarios refletem, de maneira geral, posicoes de extremidades, isto e, ou inteiramente positivas ou inteiramente negativas. Credita‑se tal situação a um carater polarizador atribuido de modo muito amplo pela sociedade brasileira ao ambiente virutal.Through the analysis of all Brazilian indigenous lands registered in said application, it is sought to investigate which concepts and categories are put into play by the social actors to refer to and analyze the Brazilian Indigenous Lands as tourist attractions. It is also important to analyze the way tourists refer to indigenous peoples in their publications, as well as the construction of stereotypes and ways of thinking about indianity. It is believed that this analysis contributes important contributions to the scientific context of tourism research. Both for using relatively new means for conducting research, and for addressing the topic of relatively undeveloped literature in the area, that is, tourism in Indigenous Lands

a produção gráfica da Impressão Régia (1768-1800)

UID/HIS/04666/2019A fundação da Régia Oficina Tipográfica, nos últimos dias de 1768, integra-se, como se sabe, numa estratégia mais ampla que configura o programa josefino das Luzes, seguindo, aliás, cronologicamente a criação da Real Mesa Censória com a qual manteve estreita ligação. O decreto fundacional de 24 de dezembro enfatiza, de forma clara a dependência direta do rei, ainda que, no articulado seja óbvio que a nova oficina tipográfica tinha liberdade para decidir sobre o seu plano editorial, numa política explícita de preços justos sem atenção a “grandes interesses de lucro” antes buscando nos seus produtos uma elevada qualidade gráfica. Os critérios editoriais de uma oficina tipográfica que era “régia” mas concorria com outras revelam-se nos 1554 livros e folhetos que, entre 1768 e 1800, deu à estampa em primeiras edições ou reimpressões. Desde a obra de grande aparato, com profusão de gravuras, para a qual a Impressão Régia estava particularmente habilitada, passando por “obras de regime” e de grande divulgação religiosa, cultural e científica até aos libretos, poesias, catálogos e gazetas, a atividade tipográfica mostra-se variada e testemunhal quanto aos gostos e necessidades dos prospetivos leitores. Nesta comunicação procuraremos apresentar 1) os dados bibliométricos que resultam de uma análise circunstanciada dos Livros de Registo existentes no Arquivo da Imprensa Nacional; 2) as opções editoriais que resultaram de encomendas privadas, coletivas e régias 3) as diferentes tipologias temáticas e, no limite, a perceção dos poderes e saberes que se entrecruzam nos reinados de D. José I e de D. Maria I.publishersversionpublishe

Variable positive end-expiratory pressure can maintain oxygenation in experimental acute respiratory distress syndrome induced by oleic acid in dogs

The use of positive end-expiratory pressure (PEEP) or lung recruitment maneuvers (RM) to improve oxygenation in acute respiratory distress syndrome (ARDS) is used but it may reduce cardiac output (CO). Intermittent PEEP may avoid these complications. Our objective was to determine if variable PEEP compared with constant PEEP is capable of maintaining arterial oxygenation and minimizing hemodynamic alterations with or without RM. Eighteen dogs with ARDS induced by oleic acid were randomized into three equal groups: group 1, low variable PEEP; group 2, high variable PEEP, and group 3, RM + high variable PEEP. All groups were submitted to constant PEEP, followed by variable PEEP (PEEP was increased from 5 to 10 cmH2O in group 1, and from 5 to 18 cmH2O in the other two groups). PaO2 was higher in group 3 (356.2 ± 65.4 mmHg) than in group 1 (92.7 ± 29.7 mmHg) and group 2 (228.5 ± 72.4 mmHg), P 0.05. Variable PEEP is able to maintain PaO2 when performed in combination with RM in dogs with ARDS. After RM, CO was reduced and there was no relevant difference between the variable and constant PEEP periods.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MedicinaCentro Universitário São CamiloUniversidade de São Paulo Faculdade de Medicina Laboratório de Investigação Médica (LIM 12)UNIFESP, EPM, Depto. de MedicinaSciEL

Criação do Centro Brasileiro de Mindful Eating: resgate da consciência e do amor ao comer

Objetivo: descrever a trajetória da criação do Centro Brasileiro de Mindful Eating e buscar compreender as motivações, os objetivos, os resultados já alcançados e as perspectivas e desafios para o futuro dessa organização. Método: estudo descritivo, exploratório com delineamento qualitativo. A coleta de dados foi realizada por meio de consultas aos documentos disponíveis no site do Centro e relatos de profissionais envolvidos em sua idealização e criação. As informações reunidas foram organizadas em categorias temáticas para oferecer descrição consistente do referido Centro enquanto marco referencial para o movimento científico e profissional do comer consciente. Resultados e Discussão: a criação do Centro compreendeu: Organização e estrutura: independência, descentralização e horizontalidade; Objetivos e motivações: amor, cuidado e ética; Desafios e perspectivas futuras: plantar, cuidar e colher. Tais categorias, exploradas em conjunto, revelaram a importância da criação do Centro ao colaborar, entre outras questões, para o estabelecimento do tema no Brasil. Conclusão: o Centro tem colaborado para o desenvolvimento profissional ético dessa área de conhecimento no Brasil, estabelecendo-se no sentido de organizar e oferecer referência para disseminação das referidas práticas. Estudos baseados nessa temática poderão contribuir para que o Mindful Eating se fortaleça enquanto importante recurso para a saúde e qualidade de vida.Objective: to describe the trajectory of the creation of the Brazilian Center of Mindful Eating and to attempt to understand the motivations, objectives, the already obtained results, as well as the future perspectives and challenges of this organization. Method: exploratory descriptive study with a qualitative design. Data collection was carried out by consulting the documents available on the Center’s website and compiling the accounts given by the professionals involved in conceiving and creating the Center. The obtained information was sorted into categories so as to provide a consistent description of said Center as a milestone for the mindful eating scientific and professional movement. Results and Discussion: creating the Center required: Organization and structure: independence, decentralization and horizontality; Objectives and motivations: love, care and ethics; Challenges and future perspectives: cultivating, taking care of and reaping the benefits of it. Those categories, explored together, have shown how important it was to create the Center to cooperate, among other things, to inaugurate the topic in Brazil. Conclusion: the Center has been contributing to the ethical professional development of this field of knowledge in Brazil and has been establishing itself so as to organize and be the reference to disseminate such practices...Objetivo: describir la trayectoria de la creación del Centro Brasileño de Mindful Eating y buscar comprender los motivos, los objetivos, los resultados ya alcanzados y las perspectivas y desafíos para el futuro de esta organización. Metodología: estudio descriptivo, exploratorio con enfoque cualitativo. La recolección de datos se realizó a través de consultas a los documentos disponibles en la página web del Centro y relatos de profesionales involucrados en su creación. La información recabada fue organizada en categorías temáticas para ofrecer una descripción consistente de dicho Centro como marco referencial para el movimiento científico y profesional del comer consciente. Resultados y Discusión: la creación del Centro involucró: Organización y estructura: independencia, descentralización y horizontalidad; Objetivos y motivos: amor, cuidado y ética; Desafíos y perspectivas futuras: plantar, cuidar y cosechar. Tales categorías, exploradas en conjunto, han revelado la importancia de la creación del Centro al colaborar, entre otros aspectos, al desarrollo de este tema en Brasil. Conclusión: el Centro ha contribuido al desarrollo profesional y ético de esta área de conocimiento en Brasil, estableciéndose a modo de organizar y servir de referencia para la difusión de tales prácticas..

In vitro study of the effect of Er: YAG laser irradiation on the apical sealing of different root canal sealers

OBJECTIVES: The aim of this study was to determine the effect of Er:YAG laser irradiation used to clean dentinal walls on the apical sealing of root canals filled with different types of sealers. Background Data: Laser application to the dentinal walls removed debris, rendering the root canals free of smear layers and leaving the dentinal canaliculi open. METHODS: Sixty-four maxillary canines obtained from laboratory files were instrumented with K-files (Dentsply, Maillefer, Ballaigues, Switzerland) using the crown-down technique, and irrigated with a 0.5% sodium hypochlorite solution. The specimens were divided into two groups of 32 teeth each. In group I, the teeth were instrumented and irrigated with sodium hypochlorite solution, and divided into four subgroups to be sealed with the different materials (Endofill, N-Rickert, Sealapex and Sealer 26). In group II, the root canals were subjected to Er:YAG laser irradiation (200 mJ, 7 Hz and 60 J total energy), followed by root canal sealing as in group I. RESULTS: The data showed lower levels of apical microleakage in the teeth filled with N-Rickert, Sealapex and Sealer 26 cements than in those sealed with Endofill (p ; 0.05). CONCLUSIONS: The Er:YAG laser irradiation applied to the root canal walls was not able to prevent apical microleakage.OBJETIVOS: Avaliou-se a infiltração marginal apical em caninos superiores, obturados com quatro tipos diferentes de cimento, os quais foram imersos em tinta nanquim e mantidos a uma temperatura de 37º C por 96 horas, descalcificados em solução de ácido clorídrico a 5%, desidratados em série crescente de álcoois e diafanizados em salicilato de metila. MÉTODOS: Para isto, 64 dentes de estoque foram instrumentados pela técnica "crown-down", irrigados com solução de hipoclorito de sódio a 0,5% e divididos em dois grupos experimentais. Os dentes do Grupo I foram subdivididos em quatro sub-grupos de oito elementos e obturados cada um deles com os cimentos Endofill®, N-Rickert®, Sealapex® e Sealer 26®. Os dentes do Grupo II receberam aplicação adicional de laser Er:YAG, tendo seus canais radiculares obturados como aqueles do grupo I. RESULTADOS: A infiltração marginal apical apresentou valores estatisticamente maiores e significantes (p ; 0,05), quanto à infiltração marginal apical, nos dentes do Grupo I, preparados somente com a solução de hipoclorito de sódio a 0,5%, e naqueles do Grupo II, que foram irradiados com laser Er: YAG. CONCLUSÕES: A irradiação de laser Er:YAG aplicada nas paredes do canal radicular não foi capaz de prevenir a infiltração marginal apical

Venous thromboembolism risk score during hospitalization in pregnancy: results of 10694 prospective evaluations in a clinical trial

Objectives: Hospitalization during pregnancy and childbirth increases the risk of Venous Thromboembolism Risk (VTE). This study applied a VTE risk score to all hospitalized pregnant women to ascertain its effectiveness in preventing maternal death from VTE until 3 months after discharge. Methods: In this interventional study, patients were classified as low- or high-risk according to the VTE risk score (Clinics Hospital risk score). High-risk patients (score ≥ 3) were scheduled for pharmacological Thromboprophylaxis (TPX). Interaction analysis of the main risk factors was performed using Odds Ratio (OR) and Poisson regression with robust variance. Results: The data of 10694 cases (7212 patients) were analyzed; 1626 (15.2%, 1000 patients) and 9068 (84.8%, 6212 patients) cases were classified as high-risk (score ≥ 3) and low-risk (score < 3), respectively. The main risk factors (Odds Ratio, 95% Confidence Interval) for VTE were age ≥ 35 and < 40 years (1.6, 1.4–1.8), parity ≥ 3 (3.5, 3.0–4.0), age ≥ 40 years (4.8, 4.1–5.6), multiple pregnancies (2.1, 1.7–2.5), BMI ≥ 40 kg/m2 (5.1, 4.3–6.0), severe infection (4.1, 3.3–5.1), and cancer (12.3, 8.8–17.2). There were 10 cases of VTE: 7/1636 (0.4%) and 3/9068 (0.03%) in the high- and low-risk groups, respectively. No patient died of VTE. The intervention reduced the VTE risk by 87%; the number needed to treat was 3. Conclusions: This VTE risk score was effective in preventing maternal deaths from VTE, with a low indication for TPX. Maternal age, multiparity, obesity, severe infections, multiple pregnancies, and cancer were the main risk factors for VTE

The novel p.Cys65Tyr mutation in NR5A1gene in three 46,XY siblings with normal testosterone levels and their mother with primary ovarian insufficiency

Disorders of sex development (DSD) is the term used for congenital conditions in which development of chromosomal, gonadal, or phenotypic sex is atypical. Nuclear receptor subfamily 5, group A, member 1 gene (NR5A1) encodes steroidogenic factor 1 (SF1), a transcription factor that is involved in gonadal development and regulates adrenal steroidogenesis. Mutations in the NR5A1 gene may lead to different 46,XX or 46,XY DSD phenotypes with or without adrenal failure. We report a Brazilian family with a novel NR5A1 mutation causing ambiguous genitalia in 46,XY affected individuals without Müllerian derivatives and apparently normal Leydig function after birth and at puberty, respectively. Their mother, who is also heterozygous for the mutation, presents evidence of primary ovarian insufficiency. Three siblings with 46,XY DSD, ambiguous genitalia and normal testosterone production were included in the study. Molecular analyses for AR, SRD5A2 genes did not reveal any mutation. However, NR5A2 sequence analysis indicated that all three siblings were heterozygous for the p.Cys65Tyr mutation which was inherited from their mother. In silico analysis was carried out to elucidate the role of the amino acid change on the protein function. After the mutation was identified, all sibs and the mother had been reevaluated. Basal hormone concentrations were normal except that ACTH levels were slightly elevated. After 1 mcg ACTH stimulation test, only the older sib showed subnormal cortisol response. The p.Cys65Tyr mutation located within the second zinc finger of DNA binding domain was considered deleterious upon analysis with predictive algorithms. The identification of heterozygous individuals with this novel mutation may bring additional knowledge on structural modifications that may influence NR5A1 DNA-binding ability, and may also contribute to genotype-phenotype correlations in DSD. The slightly elevated ACTH basal levels in all three patients with 46,XY DSD and the subnormal cortisol response after 1 mcg ACTH stimulation in the older sib indicate that a long-term follow-up for adrenal function is important for these patients. Our data reinforce that NR5A1 analysis must also be performed in 46,XY DSD patients with normal testosterone levels without AR mutations.15