VIAJANTES DO IMAGINÁRIO: A AMÉRICA VISTA DA EUROPA, SÉC. XV-XVII

VIAJANTES DO IMAGINÁRIO: A AMÉRICA VISTA DA EUROPA, SÉC. XV-XVI

Microlearning para la formación y la docencia en el Grado de Farmacia

Memoria ID-022 Ayudas de la Universidad de Salamanca para la innovación docente, curso 2020-2021

O impacto do tratamento da dor em pacientes com fibromialgia / The impact of pain management in patients with fibromyalgia

A Fibromialgia é uma doença reumatológica caracterizada por um intenso estado inflamatório que acomete o sistema muscular e neurológico cuja principal manifestação é uma dor com padrão inespecífico, contribuindo para o desenvolvimento de várias comorbidades que geram incapacidade ao indivíduo. Logo, a temática foi escolhida devido à importância de se elucidar os principais benefícios e riscos ao se submeter o paciente ao tratamento de dor com o intuito de mostrar a eficácia desse método visando estabelecer a qualidade de vida.  O presente artigo, trata-se de uma revisão bibliográfica. Foram utilizadas as bibliotecas virtuais Scielo, Google Acadêmico, Pubmed e UpToDate. Dentre os artigos pesquisados durante 3 meses, foram selecionados 20, datados entre 2017 e 2022. Os critérios de inclusão na amostra de análise foram: 1) artigos com data de publicação a partir de 2017; 2) artigos reconhecidos por especialistas na área de reumatologia. Os critérios de exclusão foram: artigos duplicados, monografias, trabalhos de conclusão de curso e capítulos de livro. Assim, grande parte das pesquisas sugerem que os sinais de dor se expandem para outros segmentos corporais sem nenhum padrão neural ou vascular, muitas vezes distal para proximal ou de um hemicorpo para o outro que por muitas vezes dificulta uma análise clínica mais precisa e assim impossibilita a adoção de medidas intervencionistas eficazes. Desse modo, a síndrome álgica pode conter vários significados a depender do indivíduo portador da doença, visto que isso implica no aumento da sensibilidade à resposta álgica tanto por distúrbios psicológicos quanto por alterações neuroendócrinas. Portanto, a fibromialgia é uma patologia que envolve uma atenção diferenciada ao paciente tendo como preceito o atendimento humanizado através de uma terapia multiprofissional tratando a dor em todos os seus aspectos e assegurando a qualidade de vida e o bem estar individual

Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi

© 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2 ) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalization (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-031158 – PTDC/BII-BIO/31158/2017. The authors would like to thank the participation and scientific support of the Unit projects UIDB/50006/2020 | UIDP/50006/2020, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universal Faixa ‘B’ (grant number 32103/2018-0). A.P. is a recipient of a post-doctoral grant from the project PTDC/BII-BIO/31158/2017. The authors would like to thank the researcher Roberto Resendes (CiBio, University of the Azores, Ponta Delgada, São Miguel, Azores, Portugal) for the logistical support in the collection of samples. C.P.A acknowledges FCT-MCTES fellowship PD/BD/136860/2018. A.B.-N. and F.C.D.A.L. acknowledge CNPq (grants 420449/2018-3 and 428211/2018-6) for financial support.info:eu-repo/semantics/publishedVersio

Inicio de la gestión de la Farmacia de AUSAF (Aula de Atención Farmacéutica de la Universidad de Salamanca)

Memoria ID-204. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019

Enhanced immunogenicity and protective efficacy in mice following a Zika DNA vaccine designed by modulation of membrane-anchoring regions and its association to adjuvants

Zika virus (ZIKV) is a re-emerging pathogen with high morbidity associated to congenital infection. Despite the scientific advances since the last outbreak in the Americas, there are no approved specific treatment or vaccines. As the development of an effective prophylactic approach remains unaddressed, DNA vaccines surge as a powerful and attractive candidate due to the efficacy of sequence optimization in achieving strong immune response. In this study, we developed four DNA vaccine constructs encoding the ZIKV prM/M (pre-membrane/membrane) and E (envelope) proteins in conjunction with molecular adjuvants. The DNA vaccine candidate (called ZK_ΔSTP), where the entire membrane-anchoring regions were completely removed, was far more immunogenic compared to their counterparts. Furthermore, inclusion of the tPA-SP leader sequence led to high expression and secretion of the target vaccine antigens, therefore contributing to adequate B cell stimulation. The ZK_ΔSTP vaccine induced high cellular and humoral response in C57BL/6 adult mice, which included high neutralizing antibody titers and the generation of germinal center B cells. Administration of ZK-ΔSTP incorporating aluminum hydroxide (Alum) adjuvant led to sustained neutralizing response. In consistency with the high and long-term protective response, ZK_ΔSTP+Alum protected adult mice upon viral challenge. Collectively, the ZK_ΔSTP+Alum vaccine formulation advances the understanding of the requirements for a successful and protective vaccine against flaviviruses and is worthy of further translational studies

EducaFarma 7.0

Memoria ID-013. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019